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Protein

Colicin-M

Gene

cma

Organism
Escherichia coli
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Colicins are polypeptide toxins produced by and active against E.coli and closely related bacteria.
This is a calcium-requiring inhibitor for murein biosynthesis; it causes lysis of sensitive cells accompanied by murein degradation. The target site is possibly the cytoplasmic membrane.

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Antibiotic, Antimicrobial, Bacteriocin

Names & Taxonomyi

Protein namesi
Recommended name:
Colicin-M
Gene namesi
Name:cma
Encoded oniPlasmid ColBM-Cl1390 Publication
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 271271Colicin-MPRO_0000218689Add
BLAST

Interactioni

Protein-protein interaction databases

IntActiP05820. 1 interaction.

Structurei

Secondary structure

1
271
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni11 – 133Combined sources
Helixi18 – 214Combined sources
Helixi38 – 458Combined sources
Helixi49 – 6517Combined sources
Beta strandi67 – 693Combined sources
Helixi70 – 9223Combined sources
Helixi98 – 1047Combined sources
Helixi109 – 1113Combined sources
Helixi114 – 1174Combined sources
Beta strandi120 – 1223Combined sources
Helixi128 – 13811Combined sources
Beta strandi144 – 1463Combined sources
Helixi148 – 1503Combined sources
Helixi157 – 1593Combined sources
Helixi161 – 1688Combined sources
Beta strandi173 – 18412Combined sources
Helixi185 – 1873Combined sources
Helixi190 – 1956Combined sources
Beta strandi198 – 20912Combined sources
Beta strandi213 – 23119Combined sources
Turni232 – 2343Combined sources
Helixi237 – 24913Combined sources
Beta strandi255 – 2584Combined sources
Beta strandi263 – 2708Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2XMXX-ray1.67A/B1-271[»]
2XTQX-ray2.31A/B/C/D/E/F/G/H1-271[»]
2XTRX-ray2.14A/B1-271[»]
3DA3X-ray2.50A/B1-271[»]
3DA4X-ray1.70A/B1-271[»]
ProteinModelPortaliP05820.
SMRiP05820. Positions 2-271.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP05820.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi2 – 98TonB box

Keywords - Domaini

TonB box

Family and domain databases

InterProiIPR028056. Colicin_M.
[Graphical view]
PfamiPF14859. Colicin_M. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P05820-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
METLTVHAPS PSTNLPSYGN GAFSLSAPHV PGAGPLLVQV VYSFFQSPNM
60 70 80 90 100
CLQALTQLED YIKKHGASNP LTLQIISTNI GYFCNADRNL VLHPGISVYD
110 120 130 140 150
AYHFAKPAPS QYDYRSMNMK QMSGNVTTPI VALAHYLWGN GAERSVNIAN
160 170 180 190 200
IGLKISPMKI NQIKDIIKSG VVGTFPVSTK FTHATGDYNV ITGAYLGNIT
210 220 230 240 250
LKTEGTLTIS ANGSWTYNGV VRSYDDKYDF NASTHRGIIG ESLTRLGAMF
260 270
SGKEYQILLP GEIHIKESGK R
Length:271
Mass (Da):29,483
Last modified:November 1, 1988 - v1
Checksum:iB41B7BE107EC1DBA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M16754 Genomic DNA. Translation: AAA23589.1.
PIRiA27090. IKECM.
RefSeqiWP_000449473.1. NZ_LQWB01000076.1.
YP_008998009.1. NC_023315.1.

Genome annotation databases

GeneIDi18194796.
KEGGipg:18194796.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M16754 Genomic DNA. Translation: AAA23589.1.
PIRiA27090. IKECM.
RefSeqiWP_000449473.1. NZ_LQWB01000076.1.
YP_008998009.1. NC_023315.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2XMXX-ray1.67A/B1-271[»]
2XTQX-ray2.31A/B/C/D/E/F/G/H1-271[»]
2XTRX-ray2.14A/B1-271[»]
3DA3X-ray2.50A/B1-271[»]
3DA4X-ray1.70A/B1-271[»]
ProteinModelPortaliP05820.
SMRiP05820. Positions 2-271.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP05820. 1 interaction.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi18194796.
KEGGipg:18194796.

Miscellaneous databases

EvolutionaryTraceiP05820.

Family and domain databases

InterProiIPR028056. Colicin_M.
[Graphical view]
PfamiPF14859. Colicin_M. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Primary structure of colicin M, an inhibitor of murein biosynthesis."
    Koeck J., Oelschlaeger T., Kamp R.M., Braun V.
    J. Bacteriol. 169:3358-3361(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiCEAM_ECOLX
AccessioniPrimary (citable) accession number: P05820
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: November 1, 1988
Last modified: May 11, 2016
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

This colicin requires TonB for its uptake.

Keywords - Technical termi

3D-structure, Plasmid

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.