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Protein

Colicin-B

Gene

cba

Organism
Escherichia coli
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

This colicin is a channel-forming colicin. This class of transmembrane toxins depolarize the cytoplasmic membrane, leading to dissipation of cellular energy.
Colicins are polypeptide toxins produced by and active against E.coli and closely related bacteria.

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Antibiotic, Antimicrobial, Bacteriocin

Protein family/group databases

TCDBi1.C.1.3.2. the channel-forming colicin (colicin) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Colicin-B
Gene namesi
Name:cba
Encoded oniPlasmid ColBM-pF1660 Publication
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei455 – 475HelicalSequence analysisAdd BLAST21
Transmembranei477 – 497HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved
ChainiPRO_00002186872 – 511Colicin-BAdd BLAST510

Structurei

Secondary structure

1511
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi12 – 17Combined sources6
Beta strandi20 – 22Combined sources3
Beta strandi50 – 52Combined sources3
Beta strandi62 – 64Combined sources3
Beta strandi70 – 73Combined sources4
Turni74 – 81Combined sources8
Beta strandi87 – 90Combined sources4
Helixi99 – 110Combined sources12
Beta strandi121 – 128Combined sources8
Beta strandi139 – 146Combined sources8
Helixi147 – 150Combined sources4
Helixi155 – 157Combined sources3
Beta strandi161 – 176Combined sources16
Beta strandi179 – 196Combined sources18
Beta strandi198 – 201Combined sources4
Beta strandi208 – 216Combined sources9
Beta strandi220 – 222Combined sources3
Beta strandi225 – 228Combined sources4
Turni230 – 233Combined sources4
Beta strandi252 – 256Combined sources5
Beta strandi259 – 262Combined sources4
Beta strandi266 – 271Combined sources6
Beta strandi281 – 286Combined sources6
Helixi291 – 340Combined sources50
Helixi342 – 357Combined sources16
Helixi366 – 376Combined sources11
Helixi386 – 398Combined sources13
Helixi401 – 406Combined sources6
Beta strandi409 – 413Combined sources5
Beta strandi415 – 417Combined sources3
Helixi420 – 435Combined sources16
Beta strandi436 – 438Combined sources3
Helixi441 – 452Combined sources12
Helixi457 – 472Combined sources16
Helixi479 – 495Combined sources17
Helixi500 – 508Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1RH1X-ray2.50A2-511[»]
ProteinModelPortaliP05819.
SMRiP05819.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP05819.

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi17 – 24TonB box8

Sequence similaritiesi

Belongs to the channel forming colicin family.Curated

Keywords - Domaini

TonB box, Transmembrane, Transmembrane helix

Family and domain databases

Gene3Di1.10.490.30. 1 hit.
InterProiIPR000293. Channel_colicin_C.
IPR016128. Pyosin/cloacin_T_dom.
[Graphical view]
PfamiPF03515. Cloacin. 1 hit.
PF01024. Colicin. 1 hit.
[Graphical view]
PRINTSiPR00280. CHANLCOLICIN.
ProDomiPD002657. Channel_colicin_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF69369. SSF69369. 1 hit.
PROSITEiPS00276. CHANNEL_COLICIN. 1 hit.
PS00430. TONB_DEPENDENT_REC_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P05819-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDNEGSVPT EGIDYGDTMV VWPSTGRIPG GDVKPGGSSG LAPSMPPGWG
60 70 80 90 100
DYSPQGIALV QSVLFPGIIR RIILDKELEE GDWSGWSVSV HSPWGNEKVS
110 120 130 140 150
AARTVLENGL RGGLPEPSRP AAVSFARLEP ASGNEQKIIR LMVTQQLEQV
160 170 180 190 200
TDIPASQLPA AGNNVPVKYR LTDLMQNGTQ YMAIIGGIPM TVPVVDAVPV
210 220 230 240 250
PDRSRPGTNI KDVYSAPVSP NLPDLVLSVG QMNTPVRSNP EIQEDGVISE
260 270 280 290 300
TGNYVEAGYT MSSNNHDVIV RFPEGSGVSP LYISAVEILD SNSLSQRQEA
310 320 330 340 350
ENNAKDDFRV KKEQENDEKT VLTKTSEVII SVGDKVGEYL GDKYKALSRE
360 370 380 390 400
IAENINNFQG KTIRSYDDAM SSINKLMANP SLKINATDKE AIVNAWKAFN
410 420 430 440 450
AEDMGNKFAA LGKTFKAADY AIKANNIREK SIEGYQTGNW GPLMLEVESW
460 470 480 490 500
VISGMASAVA LSLFSLTLGS ALIAFGLSAT VVGFVGVVIA GAIGAFIDDK
510
FVDELNHKII K
Length:511
Mass (Da):54,863
Last modified:January 23, 2007 - v3
Checksum:i8ABB972CF1925964
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M16816 Genomic DNA. Translation: AAA98063.1.
PIRiA27089. IKECBB.
RefSeqiWP_001282376.1. NZ_LXYC01000059.1.
YP_008998007.1. NC_023315.1.

Genome annotation databases

GeneIDi18195105.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M16816 Genomic DNA. Translation: AAA98063.1.
PIRiA27089. IKECBB.
RefSeqiWP_001282376.1. NZ_LXYC01000059.1.
YP_008998007.1. NC_023315.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1RH1X-ray2.50A2-511[»]
ProteinModelPortaliP05819.
SMRiP05819.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

TCDBi1.C.1.3.2. the channel-forming colicin (colicin) family.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi18195105.

Miscellaneous databases

EvolutionaryTraceiP05819.

Family and domain databases

Gene3Di1.10.490.30. 1 hit.
InterProiIPR000293. Channel_colicin_C.
IPR016128. Pyosin/cloacin_T_dom.
[Graphical view]
PfamiPF03515. Cloacin. 1 hit.
PF01024. Colicin. 1 hit.
[Graphical view]
PRINTSiPR00280. CHANLCOLICIN.
ProDomiPD002657. Channel_colicin_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF69369. SSF69369. 1 hit.
PROSITEiPS00276. CHANNEL_COLICIN. 1 hit.
PS00430. TONB_DEPENDENT_REC_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCEAB_ECOLX
AccessioniPrimary (citable) accession number: P05819
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 108 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

This colicin requires TonB for its uptake.

Keywords - Technical termi

3D-structure, Direct protein sequencing, Plasmid

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.