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Protein

Colicin-B

Gene

cba

Organism
Escherichia coli
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

This colicin is a channel-forming colicin. This class of transmembrane toxins depolarize the cytoplasmic membrane, leading to dissipation of cellular energy.
Colicins are polypeptide toxins produced by and active against E.coli and closely related bacteria.

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Antibiotic, Antimicrobial, Bacteriocin

Protein family/group databases

TCDBi1.C.1.3.2. the channel-forming colicin (colicin) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Colicin-B
Gene namesi
Name:cba
Encoded oniPlasmid ColBM-pF1660 Publication
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei455 – 47521HelicalSequence analysisAdd
BLAST
Transmembranei477 – 49721HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved
Chaini2 – 511510Colicin-BPRO_0000218687Add
BLAST

Structurei

Secondary structure

1
511
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi12 – 176Combined sources
Beta strandi20 – 223Combined sources
Beta strandi50 – 523Combined sources
Beta strandi62 – 643Combined sources
Beta strandi70 – 734Combined sources
Turni74 – 818Combined sources
Beta strandi87 – 904Combined sources
Helixi99 – 11012Combined sources
Beta strandi121 – 1288Combined sources
Beta strandi139 – 1468Combined sources
Helixi147 – 1504Combined sources
Helixi155 – 1573Combined sources
Beta strandi161 – 17616Combined sources
Beta strandi179 – 19618Combined sources
Beta strandi198 – 2014Combined sources
Beta strandi208 – 2169Combined sources
Beta strandi220 – 2223Combined sources
Beta strandi225 – 2284Combined sources
Turni230 – 2334Combined sources
Beta strandi252 – 2565Combined sources
Beta strandi259 – 2624Combined sources
Beta strandi266 – 2716Combined sources
Beta strandi281 – 2866Combined sources
Helixi291 – 34050Combined sources
Helixi342 – 35716Combined sources
Helixi366 – 37611Combined sources
Helixi386 – 39813Combined sources
Helixi401 – 4066Combined sources
Beta strandi409 – 4135Combined sources
Beta strandi415 – 4173Combined sources
Helixi420 – 43516Combined sources
Beta strandi436 – 4383Combined sources
Helixi441 – 45212Combined sources
Helixi457 – 47216Combined sources
Helixi479 – 49517Combined sources
Helixi500 – 5089Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1RH1X-ray2.50A2-511[»]
ProteinModelPortaliP05819.
SMRiP05819. Positions 10-510.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP05819.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi17 – 248TonB box

Sequence similaritiesi

Belongs to the channel forming colicin family.Curated

Keywords - Domaini

TonB box, Transmembrane, Transmembrane helix

Family and domain databases

Gene3Di1.10.490.30. 1 hit.
InterProiIPR000293. Channel_colicin_C.
IPR016128. Pyosin/cloacin_T_dom.
[Graphical view]
PfamiPF03515. Cloacin. 1 hit.
PF01024. Colicin. 1 hit.
[Graphical view]
PRINTSiPR00280. CHANLCOLICIN.
ProDomiPD002657. Channel_colicin_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF69369. SSF69369. 1 hit.
PROSITEiPS00276. CHANNEL_COLICIN. 1 hit.
PS00430. TONB_DEPENDENT_REC_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P05819-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDNEGSVPT EGIDYGDTMV VWPSTGRIPG GDVKPGGSSG LAPSMPPGWG
60 70 80 90 100
DYSPQGIALV QSVLFPGIIR RIILDKELEE GDWSGWSVSV HSPWGNEKVS
110 120 130 140 150
AARTVLENGL RGGLPEPSRP AAVSFARLEP ASGNEQKIIR LMVTQQLEQV
160 170 180 190 200
TDIPASQLPA AGNNVPVKYR LTDLMQNGTQ YMAIIGGIPM TVPVVDAVPV
210 220 230 240 250
PDRSRPGTNI KDVYSAPVSP NLPDLVLSVG QMNTPVRSNP EIQEDGVISE
260 270 280 290 300
TGNYVEAGYT MSSNNHDVIV RFPEGSGVSP LYISAVEILD SNSLSQRQEA
310 320 330 340 350
ENNAKDDFRV KKEQENDEKT VLTKTSEVII SVGDKVGEYL GDKYKALSRE
360 370 380 390 400
IAENINNFQG KTIRSYDDAM SSINKLMANP SLKINATDKE AIVNAWKAFN
410 420 430 440 450
AEDMGNKFAA LGKTFKAADY AIKANNIREK SIEGYQTGNW GPLMLEVESW
460 470 480 490 500
VISGMASAVA LSLFSLTLGS ALIAFGLSAT VVGFVGVVIA GAIGAFIDDK
510
FVDELNHKII K
Length:511
Mass (Da):54,863
Last modified:January 23, 2007 - v3
Checksum:i8ABB972CF1925964
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M16816 Genomic DNA. Translation: AAA98063.1.
PIRiA27089. IKECBB.
RefSeqiWP_001282376.1. NZ_LVNH01000124.1.
YP_008998007.1. NC_023315.1.

Genome annotation databases

GeneIDi18195105.
KEGGipg:18195105.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M16816 Genomic DNA. Translation: AAA98063.1.
PIRiA27089. IKECBB.
RefSeqiWP_001282376.1. NZ_LVNH01000124.1.
YP_008998007.1. NC_023315.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1RH1X-ray2.50A2-511[»]
ProteinModelPortaliP05819.
SMRiP05819. Positions 10-510.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

TCDBi1.C.1.3.2. the channel-forming colicin (colicin) family.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi18195105.
KEGGipg:18195105.

Miscellaneous databases

EvolutionaryTraceiP05819.

Family and domain databases

Gene3Di1.10.490.30. 1 hit.
InterProiIPR000293. Channel_colicin_C.
IPR016128. Pyosin/cloacin_T_dom.
[Graphical view]
PfamiPF03515. Cloacin. 1 hit.
PF01024. Colicin. 1 hit.
[Graphical view]
PRINTSiPR00280. CHANLCOLICIN.
ProDomiPD002657. Channel_colicin_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF69369. SSF69369. 1 hit.
PROSITEiPS00276. CHANNEL_COLICIN. 1 hit.
PS00430. TONB_DEPENDENT_REC_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Nucleotide sequence of the colicin B activity gene cba: consensus pentapeptide among TonB-dependent colicins and receptors."
    Schramm E., Mende J., Braun V., Kamp R.M.
    J. Bacteriol. 169:3350-3357(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.

Entry informationi

Entry nameiCEAB_ECOLX
AccessioniPrimary (citable) accession number: P05819
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: January 23, 2007
Last modified: July 6, 2016
This is version 105 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

This colicin requires TonB for its uptake.

Keywords - Technical termi

3D-structure, Direct protein sequencing, Plasmid

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.