P05814 (CASB_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-casein

Gene names

Name:	CSN2
Synonyms:	CASB

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	226 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Important role in determination of the surface properties of the casein micelles.
Subcellular location	Secreted.
Tissue specificity	Mammary gland specific. Secreted in milk.
Post-translational modification	Form 1-P is phosphorylated once; half of the molecules are phosphorylated on Ser-24, half on Ser-25. Ref.6 Ref.7
Sequence similarities	Belongs to the beta-casein family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Milk protein
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	calcium ion transport Traceable author statement Ref.1. Source: ProtInc
Cellular_component	extracellular region Traceable author statement. Source: Reactome
Molecular_function	calcium ion binding Traceable author statement Ref.1. Source: ProtInc enzyme inhibitor activity Traceable author statement Ref.1. Source: ProtInc transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 15	15	Ref.6
	Chain	16 – 226	211	Beta-casein Ref.6		PRO_0000004475
Amino acid modifications
	Modified residue	18	1	Phosphothreonine; in form 5-P Ref.6 Ref.7
	Modified residue	21	1	Phosphoserine; in form 4-P and form 5-P Ref.6 Ref.7
	Modified residue	23	1	Phosphoserine; in form 3-P, form 4-P and form 5-P Ref.6 Ref.7
	Modified residue	24	1	Phosphoserine; in form 1-P, form 2-P, form 3-P, form 4-P and form 5-P Ref.6 Ref.7
	Modified residue	25	1	Phosphoserine; in form 1-P, form 2-P, form 3-P, form 4-P and form 5-P Ref.6 Ref.7
Experimental info
	Sequence conflict	30	1	T → P AA sequence Ref.6
	Sequence conflict	34	1	Missing in CAA39270. Ref.1
	Sequence conflict	48 – 50	3	EDE → TDQ AA sequence Ref.6
	Sequence conflict	120	1	S → Q AA sequence Ref.6
	Sequence conflict	133	1	L → V in CAA34916. Ref.3
	Sequence conflict	140	1	H → Q in CAA34916. Ref.3
	Sequence conflict	149	1	L → S AA sequence Ref.6
	Sequence conflict	173	1	Q → E AA sequence Ref.6
	Sequence conflict	182 – 184	3	QVV → EVL AA sequence Ref.6
	Sequence conflict	188	1	Q → V AA sequence Ref.6
	Sequence conflict	207	1	T → P AA sequence Ref.6
	Sequence conflict	214 – 222	9	TQPLAPVHN → PEPSTTZABH AA sequence Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P05814 [UniParc]. Last modified August 1, 1992. Version 4. Checksum: 2619C524EA1358E8	FASTA	226	25,382
	10 20 30 40 50 60 MKVLILACLV ALALARETIE SLSSSEESIT EYKQKVEKVK HEDQQQGEDE HQDKIYPSFQ 70 80 90 100 110 120 PQPLIYPFVE PIPYGFLPQN ILPLAQPAVV LPVPQPEIME VPKAKDTVYT KGRVMPVLKS 130 140 150 160 170 180 PTIPFFDPQI PKLTDLENLH LPLPLLQPLM QQVPQPIPQT LALPPQPLWS VPQPKVLPIP 190 200 210 220 QQVVPYPQRA VPVQALLLNQ ELLLNPTHQI YPVTQPLAPV HNPISV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and sequencing of a cDNA encoding human milk beta-casein." Loennerdal B., Bergstroem S., Andersson Y., Hjalmarsson K., Sundqvist A.K., Hernell O. FEBS Lett. 269:153-156(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Structure of the human beta-casein encoding gene." Hansson L., Edlund A., Johansson T., Hernell O., Stroemqvist M., Lindquist S., Loennerdal B., Bergstroem S. Gene 139:193-199(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Placenta.
[3]	Menon R.S. Submitted (OCT-1989) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Mammary gland.
[4]	"A high resolution linkage map of human 9q34.1." Kwiatkowski D.J. Submitted (DEC-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Placenta.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	"Human beta-casein. Amino acid sequence and identification of phosphorylation sites." Greenberg R., Groves M.L., Dower H.J. J. Biol. Chem. 259:5132-5138(1984) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 16-226, PHOSPHORYLATION AT THR-18; SER-21; SER-23; SER-24 AND SER-25.
[7]	"Analysis of the human casein phosphoproteome by 2-D electrophoresis and MALDI-TOF/TOF MS reveals new phosphoforms." Poth A.G., Deeth H.C., Alewood P.F., Holland J.W. J. Proteome Res. 7:5017-5027(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 18-33, PHOSPHORYLATION AT THR-18; SER-21; SER-23; SER-24 AND SER-25, MASS SPECTROMETRY.
[8]	"Human beta-casein: partial cDNA sequence and apparent polymorphism." Menon R.S., Ham R.G. Nucleic Acids Res. 17:2869-2869(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 161-226. Tissue: Mammary gland.
[9]	"Plasmin cleavage of human beta-casein." Azuma N., Yamauchi K. Biosci. Biotechnol. Biochem. 56:1140-1141(1992) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 176-215.
+	Additional computationally mapped references.

Web resources

Protein Spotlight

Of buttons, digestion and glue - Issue 16 of November 2001

Cross-references

Sequence databases
EMBL GenBank DDBJ	X55739 mRNA. Translation: CAA39270.1. AF027807 Genomic DNA. Translation: AAC82978.1. X17070 mRNA. Translation: CAA34916.1. BC069554 mRNA. Translation: AAH69554.1. BC096194 mRNA. Translation: AAH96194.1. BC096195 mRNA. Translation: AAH96195.1. BC096196 mRNA. Translation: AAH96196.1. BC096197 mRNA. Translation: AAH96197.1. X13766 mRNA. Translation: CAA32017.1.
IPI	IPI00010849.
PIR	KBHU. I53730.
RefSeq	NP_001882.1. NM_001891.2.
UniGene	Hs.2242.
3D structure databases
DisProt	DP00199.
ProteinModelPortal	P05814.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-528N.
IntAct	P05814. 7 interactions.
MINT	MINT-5010384.
STRING	9606.ENSP00000341030.
Protein family/group databases
Allergome	1064. Hom s Casein.
PTM databases
PhosphoSite	P05814.
Polymorphism databases
DMDM	115661.
Proteomic databases
PaxDb	P05814.
PRIDE	P05814.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000353151; ENSP00000341030; ENSG00000135222.
GeneID	1447.
KEGG	hsa:1447.
UCSC	uc003hes.4. human.
Organism-specific databases
CTD	1447.
GeneCards	GC04M070869.
HGNC	HGNC:2447. CSN2.
HPA	HPA041036.
MIM	115460. gene.
neXtProt	NX_P05814.
PharmGKB	PA26950.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG45871.
HOGENOM	HOG000074044.
HOVERGEN	HBG004973.
InParanoid	P05814.
OMA	VVPYPQR.
OrthoDB	EOG41NTND.
PhylomeDB	P05814.
Enzyme and pathway databases
Pathway_Interaction_DB	ptp1bpathway. Signaling events mediated by PTP1B.
Reactome	REACT_111102. Signal Transduction.
Gene expression databases
Bgee	P05814.
CleanEx	HS_CSN2.
Genevestigator	P05814.
GermOnline	ENSG00000135222. Homo sapiens.
Family and domain databases
InterPro	IPR001588. Casein. IPR016345. Casein_beta. [Graphical view]
PANTHER	PTHR11500. PTHR11500. 1 hit.
Pfam	PF00363. Casein. 1 hit. [Graphical view]
PIRSF	PIRSF002372. Beta-casein. 1 hit.
PROSITE	PS00306. CASEIN_ALPHA_BETA. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	1447.
NextBio	5947.
PMAP-CutDB	P05814.
SOURCE	Search...

Entry information

Entry name

CASB_HUMAN

Accession

Primary (citable) accession number: P05814
Secondary accession number(s): Q4VAZ9, Q9UCM5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1988
Last sequence update:	August 1, 1992
Last modified:	April 3, 2013
This is version 120 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.