Reviewed, UniProtKB/Swiss-Prot P05807 (NTP1_VACCW)

Names and origin

Protein names

Recommended name:
    Nucleoside triphosphatase I
    EC=3.6.1.15
Alternative name(s):
    Nucleoside triphosphate phosphohydrolase I
      Short name=NPH I
    Factor X

Gene names

Name:	NPH1
Ordered Locus Names:	VACWR116
ORF Names:	D11L, D15

Organism

Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR))

Taxonomic identifier

10254 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Poxviridae › Chordopoxvirinae › Orthopoxvirus

Virus host

Bos taurus (Bovine) [TaxID: 9913]

Protein attributes

Sequence length	631 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Serves two roles in transcription; it acts in concert with viral termination factor/capping enzyme to catalyze release of UUUUUNU-containing nascent RNA from the elongation complex, and it acts by itself as a polymerase elongation factor to facilitate readthrough of intrinsic pause sites.
Catalytic activity	NTP + H2O = NDP + phosphate.
Subunit structure	Monomer.
Sequence similarities	Belongs to the helicase family. NPH I subfamily. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.

Ontologies

Keywords
Biological process	Transcription
Developmental stage	Late protein
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase
Gene Ontology (GO)
Biological process	transcription Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: InterPro helicase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 631	631	Nucleoside triphosphatase I		PRO_0000099092
Regions
	Domain	42 – 204	163	Helicase ATP-binding
	Domain	367 – 532	166	Helicase C-terminal
	Nucleotide binding	55 – 62	8	ATP
	Motif	141 – 144	4	DEXH box
Experimental info
	Mutagenesis	61	1	K → A: Abolishes ATP hydrolysis.

Sequences

	Sequence		Length	Mass (Da)
	P05807-1 [UniParc]. Last modified November 1, 1988. Version 1. Checksum: 76DE4A45F0AF00D0	FASTA	631	72,310
	10 20 30 40 50 60 MSKSHAAYID YALRRTTNMP VEMMGSDVVR LKDYQHFVAR VFLGLDSMHS LLLFHETGVG 70 80 90 100 110 120 KTMTTVYILK HLKDIYTNWA IILLVKKALI EDPWMNTILR YAPEITKDCI FINYDDQNFR 130 140 150 160 170 180 NKFFTNIKTI NSKSRICVII DECHNFISKS LIKEDGKIRP TRSVYNFLSK TIALKNHKMI 190 200 210 220 230 240 CLSATPIVNS VQEFTMLVNL LRPGSLQHQS LFENKRLVDE KELVSKLGGL CSYIVNNEFS 250 260 270 280 290 300 IFDDVEGSAS FAKKTVLMRY VNMSKKQEEI YQKAKLAEIK TGISSFRILR RMATTFTFDS 310 320 330 340 350 360 FPERQNRDPG EYAQEIATLY NDFKNSLRDR EFSKSALDTF KRGELLGGDA SAADISLFTE 370 380 390 400 410 420 LKEKSVKFID VCLGILASHG KCLVFEPFVN QSGIEILLLY FKVFGISNIE FSSRTKDTRI 430 440 450 460 470 480 KAVAEFNQES NTNGECIKTC VFSSSGGEGI SFFSINDIFI LDMTWNEASL RQIVGRAIRL 490 500 510 520 530 540 NSHVLTPPER RYVNVHFIMA RLSNGMPTVD EDLFEIIQSK SKEFVQLFRV FKHTSLEWIH 550 560 570 580 590 600 ANEKDFSPID NESGWKTLVS RAIDLSSNKN ITNKLIEGTN IWYSNSNRLM SINRGFKGVD 610 620 630 GRVYDVDGNY LHDMPDNPVI KIHDGKLIYI F

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning, encoding sequence, and expression of vaccinia virus nucleic acid-dependent nucleoside triphosphatase gene." Rodriguez J.F., Kahn J.S., Esteban M. Proc. Natl. Acad. Sci. U.S.A. 83:9566-9570(1986) [PubMed: 3025846] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Nucleotide sequence and genetic map of the 16-kb vaccinia virus HindIII D fragment." Niles E.G., Condit R.C., Caro P., Davidson K., Matusick L., Seto J. Virology 153:96-112(1986) [PubMed: 3739227] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	Niles E.G. Submitted (FEB-1988) to the PIR data bank Cited for: SEQUENCE REVISION.
[4]	"Sequencing of the coding region of Vaccinia-WR to an average 9-fold redundancy and an error rate of 0.16/10kb." Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., Osborne J., Wohlhueter R. Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"A tandemly-oriented late gene cluster within the vaccinia virus genome." Weinrich S.L., Hruby D.E. Nucleic Acids Res. 14:3003-3016(1986) [PubMed: 3008103] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-288.
[6]	"Vaccinia NPH-I, a DExH-box ATPase, is the energy coupling factor for mRNA transcription termination." Deng L., Shuman S. Genes Dev. 12:538-546(1998) [PubMed: 9472022] [Abstract] Cited for: CHARACTERIZATION.
[7]	"Mutational analysis of vaccinia virus nucleoside triphosphate phosphohydrolase I, a DNA-dependent ATPase of the DExH box family." Martins A., Gross C.H., Shuman S. J. Virol. 73:1302-1308(1999) [PubMed: 9882335] [Abstract] Cited for: MUTAGENESIS.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M14629 Genomic DNA. Translation: AAA48301.1. M15058 Genomic DNA. Translation: AAA48267.1. AY243312 Genomic DNA. Translation: AAO89395.1. X03729 Genomic DNA. Translation: CAA27370.1.
PIR	NPVZ17. A94144.
RefSeq	YP_232998.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3707708.
Family and domain databases
InterPro	IPR014001. DEAD-like_N. IPR001650. DNA/RNA_helicase_C. IPR014021. Helicase_SF1/SF2_ATP-bd. IPR013676. NPHI_C. IPR000330. SNF2_N. [Graphical view]
Pfam	PF00271. Helicase_C. 1 hit. PF08469. NPHI_C. 1 hit. PF00176. SNF2_N. 1 hit. [Graphical view]
SMART	SM00487. DEXDc. 1 hit. SM00490. HELICc. 1 hit. [Graphical view]
PROSITE	PS51192. HELICASE_ATP_BIND_1. 1 hit. PS51194. HELICASE_CTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NTP1_VACCW

Accession

Primary (citable) accession number: P05807
Secondary accession number(s): P04313 , P04317, Q76ZR6, Q84138

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 20, 1987
Last sequence update:	November 1, 1988
Last modified:	June 16, 2009
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)