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P05806

- NPRE_BACCE

UniProt

P05806 - NPRE_BACCE

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Protein
Bacillolysin
Gene
npr, nprC
Organism
Bacillus cereus
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Extracellular zinc metalloprotease.

Catalytic activityi

Similar, but not identical, to that of thermolysin.

Cofactori

Binds 4 calcium ions per subunit.
Binds 1 zinc ion per subunit.

Temperature dependencei

Thermolabile.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi307 – 3071Calcium 11 Publication
Metal bindingi309 – 3091Calcium 11 Publication
Metal bindingi311 – 3111Calcium 1; via carbonyl oxygen
Metal bindingi388 – 3881Calcium 21 Publication
Metal bindingi392 – 3921Zinc; catalytic1 Publication
Active sitei393 – 3931
Metal bindingi396 – 3961Zinc; catalytic1 Publication
Metal bindingi416 – 4161Zinc; catalytic1 Publication
Metal bindingi427 – 4271Calcium 21 Publication
Metal bindingi427 – 4271Calcium 31 Publication
Metal bindingi433 – 4331Calcium 3; via carbonyl oxygen
Metal bindingi435 – 4351Calcium 21 Publication
Metal bindingi435 – 4351Calcium 31 Publication
Metal bindingi437 – 4371Calcium 2; via carbonyl oxygen
Metal bindingi440 – 4401Calcium 21 Publication
Metal bindingi440 – 4401Calcium 31 Publication
Metal bindingi443 – 4431Calcium 4; via carbonyl oxygen
Metal bindingi444 – 4441Calcium 41 Publication
Metal bindingi447 – 4471Calcium 4; via carbonyl oxygen
Metal bindingi450 – 4501Calcium 41 Publication
Active sitei481 – 4811Proton donor

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. metalloendopeptidase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Calcium, Metal-binding, Zinc

Protein family/group databases

MEROPSiM04.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Bacillolysin (EC:3.4.24.28)
Alternative name(s):
Neutral protease
Gene namesi
Name:npr
Synonyms:nprC
OrganismiBacillus cereus
Taxonomic identifieri1396 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727 Reviewed prediction
Add
BLAST
Propeptidei28 – 249222Activation peptide
PRO_0000028598Add
BLAST
Chaini250 – 566317Bacillolysin
PRO_0000028599Add
BLAST

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi253 – 2608
Beta strandi266 – 2749
Beta strandi277 – 2826
Beta strandi284 – 2874
Beta strandi289 – 29810
Beta strandi306 – 3127
Helixi315 – 3173
Helixi318 – 33821
Turni342 – 3454
Beta strandi350 – 35910
Beta strandi363 – 3653
Beta strandi367 – 3726
Beta strandi377 – 3804
Helixi383 – 3853
Helixi387 – 40014
Turni401 – 4033
Helixi409 – 42921
Beta strandi436 – 4394
Turni440 – 4423
Beta strandi445 – 4473
Beta strandi452 – 4565
Helixi458 – 4614
Helixi467 – 4693
Helixi475 – 49622
Beta strandi498 – 5003
Beta strandi503 – 5053
Helixi510 – 52314
Helixi531 – 54616
Helixi551 – 56212

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ESPX-ray2.80A250-566[»]
1NPCX-ray2.00A250-566[»]
ProteinModelPortaliP05806.
SMRiP05806. Positions 250-566.

Miscellaneous databases

EvolutionaryTraceiP05806.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M4 family.

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.10.170.10. 1 hit.
InterProiIPR011096. FTP_domain.
IPR025711. PepSY.
IPR023612. Peptidase_M4.
IPR001570. Peptidase_M4_C_domain.
IPR013856. Peptidase_M4_domain.
[Graphical view]
PfamiPF07504. FTP. 1 hit.
PF03413. PepSY. 1 hit.
PF01447. Peptidase_M4. 1 hit.
PF02868. Peptidase_M4_C. 1 hit.
[Graphical view]
PRINTSiPR00730. THERMOLYSIN.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P05806-1 [UniParc]FASTAAdd to Basket

« Hide

MKKKSLALVL ATGMAVTTFG GTGSAFADSK NVLSTKKYNE TVQSPEFISG    50
DLTEATGKKA ESVVFDYLNA AKGDYKLGEK SAQDSFKVKQ VKKDAVTDST 100
VVRMQQVYEG VPVWGSTQVA HVSKDGSLKV LSGTVAPDLD KKEKLKNKNK 150
IEGAKAIEIA QQDLGVTPKY EVEPKADLYV YQNGEETTYA YVVNLNFLDP 200
SPGNYYYFIE ADSGKVLNKF NTIDHVTNDD KSPVKQEAPK QDAKAVVKPV 250
TGTNKVGTGK GVLGDTKSLN TTLSGSSYYL QDNTRGATIF TYDAKNRSTL 300
PGTLWADADN VFNAAYDAAA VDAHYYAGKT YDYYKATFNR NSINDAGAPL 350
KSTVHYGSNY NNAFWNGSQM VYGDGDGVTF TSLSGGIDVI GHELTHAVTE 400
NSSNLIYQNE SGALNEAISD IFGTLVEFYD NRNPDWEIGE DIYTPGKAGD 450
ALRSMSDPTK YGDPDHYSKR YTGSSDNGGV HTNSGIINKQ AYLLANGGTH 500
YGVTVTGIGK DKLGAIYYRA NTQYFTQSTT FSQARAGAVQ AAADLYGANS 550
AEVAAVKQSF SAVGVN 566
Length:566
Mass (Da):60,919
Last modified:October 1, 1996 - v2
Checksum:iE18B4572C2C4E1D3
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M83910 Genomic DNA. Translation: AAA22620.1.
PIRiS22690. HYBSU.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M83910 Genomic DNA. Translation: AAA22620.1 .
PIRi S22690. HYBSU.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1ESP X-ray 2.80 A 250-566 [» ]
1NPC X-ray 2.00 A 250-566 [» ]
ProteinModelPortali P05806.
SMRi P05806. Positions 250-566.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi M04.001.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei P05806.

Family and domain databases

Gene3Di 3.10.170.10. 1 hit.
InterProi IPR011096. FTP_domain.
IPR025711. PepSY.
IPR023612. Peptidase_M4.
IPR001570. Peptidase_M4_C_domain.
IPR013856. Peptidase_M4_domain.
[Graphical view ]
Pfami PF07504. FTP. 1 hit.
PF03413. PepSY. 1 hit.
PF01447. Peptidase_M4. 1 hit.
PF02868. Peptidase_M4_C. 1 hit.
[Graphical view ]
PRINTSi PR00730. THERMOLYSIN.
PROSITEi PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The role of the pro-sequence in the processing and secretion of the thermolysin-like neutral protease from Bacillus cereus."
    Wetmore D.R., Wong S.L., Roche R.S.
    Mol. Microbiol. 6:1593-1604(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The primary structure of Bacillus cereus neutral proteinase and comparison with thermolysin and Bacillus subtilis neutral proteinase."
    Sidler W., Niederer E., Suter F., Zuber H.
    Biol. Chem. Hoppe-Seyler 367:643-657(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 250-566.
    Strain: DSM 3101.
  3. "Crystal structure of neutral protease from Bacillus cereus refined at 3.0-A resolution and comparison with the homologous but more thermostable enzyme thermolysin."
    Pauptit R.A., Karlsson R., Picot D., Jenkins J.A., Niklaus-Reimer A.-S., Jansonius J.N.
    J. Mol. Biol. 199:525-537(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS).
  4. "The structure of neutral protease from Bacillus cereus at 0.2-nm resolution."
    Stark W., Pauptit R.A., Wilson K.S., Jansonius J.N.
    Eur. J. Biochem. 207:781-791(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
  5. "E144S active-site mutant of the Bacillus cereus thermolysin-like neutral protease at 2.8-A resolution."
    Lister S.A., Wetmore D.R., Roche R.S., Codding P.W.
    Acta Crystallogr. D 52:543-550(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF MUTANT SER-393.

Entry informationi

Entry nameiNPRE_BACCE
AccessioniPrimary (citable) accession number: P05806
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: October 1, 1996
Last modified: May 14, 2014
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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