Reviewed, UniProtKB/Swiss-Prot P05804 (BGLR_ECOLI)

Names and origin

Protein names

Recommended name:
    Beta-glucuronidase
      Short name=GUS
    EC=3.2.1.31
Alternative name(s):
    Beta-D-glucuronoside glucuronosohydrolase

Gene names

Name:	uidA
Synonyms:	gurA, gusA
Ordered Locus Names:	b1617, JW1609

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	603 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	A beta-D-glucuronoside + H2O = D-glucuronate + an alcohol.
Subunit structure	Homotetramer. Ref.7
Miscellaneous	Substrates for this enzyme are generally water-soluble.
Sequence similarities	Belongs to the glycosyl hydrolase 2 family.
biophysicochemical properties	pH dependence: Optimum pH is 5.0-7.5. Temperature dependence: Resistant to thermal inactivation at 50 degrees Celsius.

Ontologies

Keywords
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	beta-glucuronidase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
nadE	P18843	1	EBI-1124641,EBI-548960

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 603	603	Beta-glucuronidase		PRO_0000057680
Sites
	Active site	413	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	P05804-1 [UniParc]. Last modified October 1, 1994. Version 2. Checksum: E769C8D61A3B9A76	FASTA	603	68,447
	10 20 30 40 50 60 MLRPVETPTR EIKKLDGLWA FSLDRENCGI DQRWWESALQ ESRAIAVPGS FNDQFADADI 70 80 90 100 110 120 RNYAGNVWYQ REVFIPKGWA GQRIVLRFDA VTHYGKVWVN NQEVMEHQGG YTPFEADVTP 130 140 150 160 170 180 YVIAGKSVRI TVCVNNELNW QTIPPGMVIT DENGKKKQSY FHDFFNYAGI HRSVMLYTTP 190 200 210 220 230 240 NTWVDDITVV THVAQDCNHA SVDWQVVANG DVSVELRDAD QQVVATGQGT SGTLQVVNPH 250 260 270 280 290 300 LWQPGEGYLY ELCVTAKSQT ECDIYPLRVG IRSVAVKGEQ FLINHKPFYF TGFGRHEDAD 310 320 330 340 350 360 LRGKGFDNVL MVHDHALMDW IGANSYRTSH YPYAEEMLDW ADEHGIVVID ETAAVGFNLS 370 380 390 400 410 420 LGIGFEAGNK PKELYSEEAV NGETQQAHLQ AIKELIARDK NHPSVVMWSI ANEPDTRPQG 430 440 450 460 470 480 AREYFAPLAE ATRKLDPTRP ITCVNVMFCD AHTDTISDLF DVLCLNRYYG WYVQSGDLET 490 500 510 520 530 540 AEKVLEKELL AWQEKLHQPI IITEYGVDTL AGLHSMYTDM WSEEYQCAWL DMYHRVFDRV 550 560 570 580 590 600 SAVVGEQVWN FADFATSQGI LRVGGNKKGI FTRDRKPKSA AFLLQKRWTG MNFGEKPQQG GKQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Beta-glucuronidase from Escherichia coli as a gene-fusion marker." Jefferson R.A., Burgess S.M., Hirsh D. Proc. Natl. Acad. Sci. U.S.A. 83:8447-8451(1986) [PubMed: 3534890] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-11.
[2]	"Nucleotide sequence corrections of the uidA open reading frame encoding beta-glucuronidase." Schlaman H.R., Risseeuw E., Franke-Van Dijk M.E., Hooykaas P.J. Gene 138:259-260(1994) [PubMed: 8125312] [Abstract] Cited for: SEQUENCE REVISION TO 279.
[3]	"Manipulation of beta-glucuronidase for use as a reporter in vacuolar targeting studies." Farrell L.B., Beachy R.N. Plant Mol. Biol. 15:821-825(1990) [PubMed: 2103475] [Abstract] Cited for: SEQUENCE REVISION TO 420-425.
[4]	"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K. , Nakade S., Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y., Wada C., Yamamoto Y., Horiuchi T. DNA Res. 3:363-377(1996) [PubMed: 9097039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[6]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[7]	"One step purification of Escherichia coli beta-glucuronidase." Blanco C., Nemoz G. Biochimie 69:157-161(1987) [PubMed: 3105604] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-5, SUBUNIT.
[8]	Jefferson R.A. Thesis (1985), University of Colorado, United States Cited for: CHARACTERIZATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M14641 Genomic DNA. Translation: AAA68923.1. Sequence problems. S69414 Genomic DNA. Translation: AAB30197.1. U00096 Genomic DNA. Translation: AAC74689.1. AP009048 Genomic DNA. Translation: BAA15368.1.
PIR	GBECGC. C64918.
RefSeq	AP_002238.1. NP_416134.1.
3D structure databases
HSSP	HSSP built from PDB template 1BHG based on UniProtKB P08236.
ModBase	Search...
Protein-protein interaction databases
IntAct	P05804. 2 interactions.
Protein family/group databases
CAZy	GH2. Glycoside Hydrolase Family 2.
Genome annotation databases
GeneID	946149.
GenomeReviews	Gene locus JW1609 in contig AP009048_GR. Gene locus b1617 in contig U00096_GR.
KEGG	ecj:JW1609. eco:b1617.
Organism-specific databases
EchoBASE	EB1048.
EcoGene	EG11055. uidA.
CMR	Search...
Phylogenomic databases
HOGENOM	P05804.
OMA	P05804. GHMEVIQ.
Enzyme and pathway databases
BioCyc	EcoCyc:BETA-GLUCURONID-MON. MetaCyc:BETA-GLUCURONID-MON.
Family and domain databases
InterPro	IPR006101. Glyco_hydro_2. IPR013812. Glyco_hydro_2/20_Ig-like. IPR006104. Glyco_hydro_2_carb-bd. IPR006102. Glyco_hydro_2_Ig-like. IPR006103. Glyco_hydro_2_TIM. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view]
Gene3D	G3DSA:2.60.40.320. Glyco_hydro_2/20_Ig-like. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF00703. Glyco_hydro_2. 1 hit. PF02836. Glyco_hydro_2_C. 1 hit. PF02837. Glyco_hydro_2_N. 1 hit. [Graphical view]
PRINTS	PR00132. GLHYDRLASE2.
PROSITE	PS00719. GLYCOSYL_HYDROL_F2_1. 1 hit. PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BGLR_ECOLI

Accession

Primary (citable) accession number: P05804

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1988
Last sequence update:	October 1, 1994
Last modified:	June 16, 2009
This is version 81 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)