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Protein

Beta-glucuronidase

Gene

uidA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

A beta-D-glucuronoside + H2O = D-glucuronate + an alcohol.

pH dependencei

Optimum pH is 5.0-7.5.

Temperature dependencei

Resistant to thermal inactivation at 50 degrees Celsius.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei413Proton donorBy similarity1

GO - Molecular functioni

  • beta-glucuronidase activity Source: EcoCyc

GO - Biological processi

  • glucuronoside catabolic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BioCyciEcoCyc:BETA-GLUCURONID-MONOMER.
ECOL316407:JW1609-MONOMER.
MetaCyc:BETA-GLUCURONID-MONOMER.
BRENDAi3.2.1.31. 2026.
SABIO-RKP05804.

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-glucuronidase (EC:3.2.1.31)
Short name:
GUS
Alternative name(s):
Beta-D-glucuronoside glucuronosohydrolase
Gene namesi
Name:uidA
Synonyms:gurA, gusA
Ordered Locus Names:b1617, JW1609
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11055. uidA.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
  • intracellular membrane-bounded organelle Source: GO_Central
Complete GO annotation...

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3217380.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000576801 – 603Beta-glucuronidaseAdd BLAST603

Proteomic databases

PaxDbiP05804.
PRIDEiP05804.

Interactioni

Subunit structurei

Homotetramer.1 Publication

Protein-protein interaction databases

BioGridi4263483. 11 interactors.
DIPiDIP-11086N.
IntActiP05804. 2 interactors.
STRINGi511145.b1617.

Chemistry databases

BindingDBiP05804.

Structurei

Secondary structure

1603
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi6 – 8Combined sources3
Beta strandi10 – 12Combined sources3
Beta strandi18 – 26Combined sources9
Turni29 – 33Combined sources5
Helixi34 – 36Combined sources3
Beta strandi43 – 49Combined sources7
Turni52 – 55Combined sources4
Helixi58 – 61Combined sources4
Beta strandi65 – 74Combined sources10
Helixi77 – 79Combined sources3
Beta strandi80 – 82Combined sources3
Beta strandi83 – 89Combined sources7
Beta strandi92 – 101Combined sources10
Beta strandi103 – 107Combined sources5
Beta strandi110 – 112Combined sources3
Beta strandi114 – 117Combined sources4
Helixi119 – 121Combined sources3
Beta strandi126 – 135Combined sources10
Beta strandi142 – 144Combined sources3
Beta strandi146 – 150Combined sources5
Beta strandi156 – 164Combined sources9
Beta strandi174 – 179Combined sources6
Beta strandi181 – 191Combined sources11
Beta strandi194 – 197Combined sources4
Beta strandi201 – 209Combined sources9
Beta strandi211 – 217Combined sources7
Beta strandi219 – 221Combined sources3
Beta strandi223 – 228Combined sources6
Beta strandi233 – 235Combined sources3
Turni244 – 246Combined sources3
Beta strandi250 – 257Combined sources8
Beta strandi262 – 269Combined sources8
Beta strandi274 – 277Combined sources4
Beta strandi280 – 283Combined sources4
Beta strandi290 – 295Combined sources6
Turni300 – 304Combined sources5
Helixi308 – 321Combined sources14
Beta strandi325 – 328Combined sources4
Helixi335 – 344Combined sources10
Beta strandi347 – 351Combined sources5
Beta strandi364 – 366Combined sources3
Beta strandi374 – 376Combined sources3
Turni377 – 379Combined sources3
Helixi382 – 399Combined sources18
Beta strandi405 – 413Combined sources9
Helixi421 – 435Combined sources15
Beta strandi437 – 439Combined sources3
Beta strandi441 – 446Combined sources6
Beta strandi447 – 449Combined sources3
Turni451 – 453Combined sources3
Helixi457 – 459Combined sources3
Beta strandi460 – 466Combined sources7
Turni469 – 471Combined sources3
Beta strandi472 – 474Combined sources3
Helixi478 – 496Combined sources19
Beta strandi500 – 504Combined sources5
Beta strandi520 – 522Combined sources3
Helixi523 – 537Combined sources15
Beta strandi543 – 552Combined sources10
Beta strandi558 – 561Combined sources4
Beta strandi562 – 566Combined sources5
Beta strandi569 – 571Combined sources3
Helixi580 – 590Combined sources11
Beta strandi598 – 600Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3K46X-ray2.50A/B1-603[»]
3K4AX-ray2.90A/B1-603[»]
3K4DX-ray2.39A/B1-603[»]
3LPFX-ray2.26A/B1-603[»]
3LPGX-ray2.42A/B1-603[»]
4JHZX-ray2.83A/B1-601[»]
5CZKX-ray2.39A/B1-603[»]
ProteinModelPortaliP05804.
SMRiP05804.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP05804.

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 2 family.Curated

Phylogenomic databases

eggNOGiENOG4105CNT. Bacteria.
COG3250. LUCA.
HOGENOMiHOG000120896.
InParanoidiP05804.
KOiK01195.
OMAiYTTPRTF.
PhylomeDBiP05804.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR008979. Galactose-bd-like.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR006103. Glyco_hydro_2_cat.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SUPFAMiSSF49303. SSF49303. 1 hit.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P05804-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRPVETPTR EIKKLDGLWA FSLDRENCGI DQRWWESALQ ESRAIAVPGS
60 70 80 90 100
FNDQFADADI RNYAGNVWYQ REVFIPKGWA GQRIVLRFDA VTHYGKVWVN
110 120 130 140 150
NQEVMEHQGG YTPFEADVTP YVIAGKSVRI TVCVNNELNW QTIPPGMVIT
160 170 180 190 200
DENGKKKQSY FHDFFNYAGI HRSVMLYTTP NTWVDDITVV THVAQDCNHA
210 220 230 240 250
SVDWQVVANG DVSVELRDAD QQVVATGQGT SGTLQVVNPH LWQPGEGYLY
260 270 280 290 300
ELCVTAKSQT ECDIYPLRVG IRSVAVKGEQ FLINHKPFYF TGFGRHEDAD
310 320 330 340 350
LRGKGFDNVL MVHDHALMDW IGANSYRTSH YPYAEEMLDW ADEHGIVVID
360 370 380 390 400
ETAAVGFNLS LGIGFEAGNK PKELYSEEAV NGETQQAHLQ AIKELIARDK
410 420 430 440 450
NHPSVVMWSI ANEPDTRPQG AREYFAPLAE ATRKLDPTRP ITCVNVMFCD
460 470 480 490 500
AHTDTISDLF DVLCLNRYYG WYVQSGDLET AEKVLEKELL AWQEKLHQPI
510 520 530 540 550
IITEYGVDTL AGLHSMYTDM WSEEYQCAWL DMYHRVFDRV SAVVGEQVWN
560 570 580 590 600
FADFATSQGI LRVGGNKKGI FTRDRKPKSA AFLLQKRWTG MNFGEKPQQG

GKQ
Length:603
Mass (Da):68,447
Last modified:October 1, 1994 - v2
Checksum:iE769C8D61A3B9A76
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14641 Genomic DNA. Translation: AAA68923.1. Sequence problems.
S69414 Genomic DNA. Translation: AAB30197.1.
U00096 Genomic DNA. Translation: AAC74689.1.
AP009048 Genomic DNA. Translation: BAA15368.1.
PIRiC64918. GBECGC.
RefSeqiNP_416134.1. NC_000913.3.
WP_000945878.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74689; AAC74689; b1617.
BAA15368; BAA15368; BAA15368.
GeneIDi946149.
KEGGiecj:JW1609.
eco:b1617.
PATRICi32118536. VBIEscCol129921_1688.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14641 Genomic DNA. Translation: AAA68923.1. Sequence problems.
S69414 Genomic DNA. Translation: AAB30197.1.
U00096 Genomic DNA. Translation: AAC74689.1.
AP009048 Genomic DNA. Translation: BAA15368.1.
PIRiC64918. GBECGC.
RefSeqiNP_416134.1. NC_000913.3.
WP_000945878.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3K46X-ray2.50A/B1-603[»]
3K4AX-ray2.90A/B1-603[»]
3K4DX-ray2.39A/B1-603[»]
3LPFX-ray2.26A/B1-603[»]
3LPGX-ray2.42A/B1-603[»]
4JHZX-ray2.83A/B1-601[»]
5CZKX-ray2.39A/B1-603[»]
ProteinModelPortaliP05804.
SMRiP05804.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263483. 11 interactors.
DIPiDIP-11086N.
IntActiP05804. 2 interactors.
STRINGi511145.b1617.

Chemistry databases

BindingDBiP05804.
ChEMBLiCHEMBL3217380.

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Proteomic databases

PaxDbiP05804.
PRIDEiP05804.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74689; AAC74689; b1617.
BAA15368; BAA15368; BAA15368.
GeneIDi946149.
KEGGiecj:JW1609.
eco:b1617.
PATRICi32118536. VBIEscCol129921_1688.

Organism-specific databases

EchoBASEiEB1048.
EcoGeneiEG11055. uidA.

Phylogenomic databases

eggNOGiENOG4105CNT. Bacteria.
COG3250. LUCA.
HOGENOMiHOG000120896.
InParanoidiP05804.
KOiK01195.
OMAiYTTPRTF.
PhylomeDBiP05804.

Enzyme and pathway databases

BioCyciEcoCyc:BETA-GLUCURONID-MONOMER.
ECOL316407:JW1609-MONOMER.
MetaCyc:BETA-GLUCURONID-MONOMER.
BRENDAi3.2.1.31. 2026.
SABIO-RKP05804.

Miscellaneous databases

EvolutionaryTraceiP05804.
PROiP05804.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR008979. Galactose-bd-like.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR006103. Glyco_hydro_2_cat.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SUPFAMiSSF49303. SSF49303. 1 hit.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBGLR_ECOLI
AccessioniPrimary (citable) accession number: P05804
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: October 1, 1994
Last modified: November 2, 2016
This is version 141 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Substrates for this enzyme are generally water-soluble.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.