P05804 (BGLR_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-glucuronidase
Short name=GUS
EC=3.2.1.31

Alternative name(s):
Beta-D-glucuronoside glucuronosohydrolase

Gene names

Name:	uidA
Synonyms:	gurA, gusA
Ordered Locus Names:	b1617, JW1609

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	603 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	A beta-D-glucuronoside + H2O = D-glucuronate + an alcohol.
Subunit structure	Homotetramer. Ref.7
Miscellaneous	Substrates for this enzyme are generally water-soluble.
Sequence similarities	Belongs to the glycosyl hydrolase 2 family.
Biophysicochemical properties	pH dependence: Optimum pH is 5.0-7.5. Temperature dependence: Resistant to thermal inactivation at 50 degrees Celsius.

Ontologies

Keywords
Molecular function	Glycosidase Hydrolase
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	glucuronoside catabolic process Inferred from mutant phenotype. Source: EcoCyc
Molecular function	beta-glucuronidase activity Inferred from direct assay Ref.7. Source: EcoCyc cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
nadE	P18843	1	EBI-1124641,EBI-548960

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 603

603

Beta-glucuronidase

PRO_0000057680

Sites

Active site

413

1

Proton donor By similarity

Secondary structure

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603

Helix Strand Turn

Details...

Beta strand

6 – 8

3

Beta strand

10 – 12

3

Beta strand

19 – 23

5

Helix

34 – 36

3

Beta strand

43 – 45

3

Turn

52 – 55

4

Helix

58 – 61

4

Beta strand

67 – 74

8

Beta strand

83 – 89

7

Beta strand

96 – 101

6

Beta strand

103 – 105

3

Beta strand

114 – 117

4

Helix

119 – 121

3

Beta strand

127 – 135

9

Beta strand

148 – 150

3

Beta strand

156 – 158

3

Beta strand

161 – 164

4

Beta strand

174 – 179

6

Beta strand

181 – 191

11

Beta strand

201 – 208

8

Beta strand

212 – 217

6

Beta strand

223 – 225

3

Beta strand

250 – 257

8

Beta strand

262 – 269

8

Beta strand

274 – 276

3

Beta strand

281 – 283

3

Helix

308 – 320

13

Beta strand

325 – 327

3

Helix

335 – 343

9

Beta strand

347 – 351

5

Beta strand

374 – 376

3

Turn

377 – 379

3

Helix

384 – 399

16

Beta strand

405 – 413

9

Helix

422 – 435

14

Beta strand

437 – 439

3

Beta strand

441 – 446

6

Turn

451 – 453

3

Helix

457 – 459

3

Beta strand

460 – 466

7

Helix

478 – 496

19

Beta strand

500 – 504

5

Helix

523 – 537

15

Helix

580 – 588

9

Sequences

	Sequence		Length	Mass (Da)
	P05804 [UniParc]. Last modified October 1, 1994. Version 2. Checksum: E769C8D61A3B9A76	FASTA	603	68,447
	10 20 30 40 50 60 MLRPVETPTR EIKKLDGLWA FSLDRENCGI DQRWWESALQ ESRAIAVPGS FNDQFADADI 70 80 90 100 110 120 RNYAGNVWYQ REVFIPKGWA GQRIVLRFDA VTHYGKVWVN NQEVMEHQGG YTPFEADVTP 130 140 150 160 170 180 YVIAGKSVRI TVCVNNELNW QTIPPGMVIT DENGKKKQSY FHDFFNYAGI HRSVMLYTTP 190 200 210 220 230 240 NTWVDDITVV THVAQDCNHA SVDWQVVANG DVSVELRDAD QQVVATGQGT SGTLQVVNPH 250 260 270 280 290 300 LWQPGEGYLY ELCVTAKSQT ECDIYPLRVG IRSVAVKGEQ FLINHKPFYF TGFGRHEDAD 310 320 330 340 350 360 LRGKGFDNVL MVHDHALMDW IGANSYRTSH YPYAEEMLDW ADEHGIVVID ETAAVGFNLS 370 380 390 400 410 420 LGIGFEAGNK PKELYSEEAV NGETQQAHLQ AIKELIARDK NHPSVVMWSI ANEPDTRPQG 430 440 450 460 470 480 AREYFAPLAE ATRKLDPTRP ITCVNVMFCD AHTDTISDLF DVLCLNRYYG WYVQSGDLET 490 500 510 520 530 540 AEKVLEKELL AWQEKLHQPI IITEYGVDTL AGLHSMYTDM WSEEYQCAWL DMYHRVFDRV 550 560 570 580 590 600 SAVVGEQVWN FADFATSQGI LRVGGNKKGI FTRDRKPKSA AFLLQKRWTG MNFGEKPQQG GKQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Beta-glucuronidase from Escherichia coli as a gene-fusion marker." Jefferson R.A., Burgess S.M., Hirsh D. Proc. Natl. Acad. Sci. U.S.A. 83:8447-8451(1986) [PubMed: 3534890] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-11.
[2]	"Nucleotide sequence corrections of the uidA open reading frame encoding beta-glucuronidase." Schlaman H.R., Risseeuw E., Franke-Van Dijk M.E., Hooykaas P.J. Gene 138:259-260(1994) [PubMed: 8125312] [Abstract] Cited for: SEQUENCE REVISION TO 279.
[3]	"Manipulation of beta-glucuronidase for use as a reporter in vacuolar targeting studies." Farrell L.B., Beachy R.N. Plant Mol. Biol. 15:821-825(1990) [PubMed: 2103475] [Abstract] Cited for: SEQUENCE REVISION TO 420-425.
[4]	"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K. , Nakade S., Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y., Wada C., Yamamoto Y., Horiuchi T. DNA Res. 3:363-377(1996) [PubMed: 9097039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[6]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[7]	"One step purification of Escherichia coli beta-glucuronidase." Blanco C., Nemoz G. Biochimie 69:157-161(1987) [PubMed: 3105604] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-5, SUBUNIT.
[8]	Jefferson R.A. Thesis (1985), University of Colorado, United States Cited for: CHARACTERIZATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M14641 Genomic DNA. Translation: AAA68923.1. Sequence problems.
S69414 Genomic DNA. Translation: AAB30197.1.
U00096 Genomic DNA. Translation: AAC74689.1.
AP009048 Genomic DNA. Translation: BAA15368.1.

PIR

GBECGC. C64918.

RefSeq

NP_416134.1. NC_000913.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3K46	X-ray	2.50	A/B	1-603	[»]
3K4A	X-ray	2.90	A/B	1-603	[»]
3K4D	X-ray	2.39	A/B	1-603	[»]
3LPF	X-ray	2.26	A/B	1-603	[»]
3LPG	X-ray	2.42	A/B	1-603	[»]

ProteinModelPortal

P05804.

SMR

P05804. Positions 1-601.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-11086N.

IntAct

P05804. 2 interactions.

Protein family/group databases

CAZy

GH2. Glycoside Hydrolase Family 2.

Proteomic databases

PRIDE

P05804.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBESCT00000003130; EBESCP00000003130; EBESCG00000002566.
EBESCT00000016677; EBESCP00000015968; EBESCG00000015736.

GeneID

946149.

GenomeReviews

Gene locus JW1609 in contig AP009048_GR.
Gene locus b1617 in contig U00096_GR.

KEGG

ecj:JW1609.
eco:b1617.

PATRIC

32118536. VBIEscCol129921_1688.

Organism-specific databases

EchoBASE

EB1048.

EcoGene

EG11055. uidA.

Phylogenomic databases

eggNOG

COG3250.

GeneTree

EBGT00050000009399.

HOGENOM

HBG474923.

OMA

IADVFIN.

PhylomeDB

P05804.

ProtClustDB

PRK10150.

Enzyme and pathway databases

BioCyc

EcoCyc:BETA-GLUCURONID-MONOMER.
MetaCyc:BETA-GLUCURONID-MONOMER.

Gene expression databases

Genevestigator

P05804.

Family and domain databases

InterPro

IPR008979. Galactose-bd-like.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_subgr_catalytic.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]

Gene3D

G3DSA:2.60.40.320. Glyco_hydro_2/20_Ig-like. 1 hit.
G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.

KO

K01195.

Pfam

PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]

PRINTS

PR00132. GLHYDRLASE2.

SUPFAM

SSF49785. Gal_bind_like. 1 hit.
SSF49303. Glyco_hydro_2Ig. 1 hit.
SSF51445. Glyco_hydro_cat. 1 hit.

PROSITE

PS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

BGLR_ECOLI

Accession

Primary (citable) accession number: P05804

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1988
Last sequence update:	October 1, 1994
Last modified:	January 25, 2012
This is version 103 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program