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Protein

Guanyl-specific ribonuclease Sa

Gene

rnaSA

Organism
Streptomyces aureofaciens
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Two-stage endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in G-P with 2',3'-cyclic phosphate intermediates.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei54Proton acceptor1
Active sitei85Proton donor1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Enzyme and pathway databases

BRENDAi3.1.27.3. 5978.

Names & Taxonomyi

Protein namesi
Recommended name:
Guanyl-specific ribonuclease Sa (EC:3.1.27.3)
Short name:
RNase Sa
Gene namesi
Name:rnaSA
OrganismiStreptomyces aureofaciens
Taxonomic identifieri1894 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomyces

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi39N → A, D or S: Decreases protein stability. 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001373671 – 96Guanyl-specific ribonuclease SaAdd BLAST96

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi7 ↔ 96

Keywords - PTMi

Disulfide bond

Interactioni

Protein-protein interaction databases

IntActiP05798. 1 interactor.
MINTiMINT-1539638.

Structurei

Secondary structure

196
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi5 – 7Combined sources3
Helixi8 – 10Combined sources3
Helixi13 – 23Combined sources11
Turni31 – 34Combined sources4
Beta strandi35 – 37Combined sources3
Beta strandi42 – 44Combined sources3
Beta strandi48 – 50Combined sources3
Beta strandi51 – 56Combined sources6
Beta strandi63 – 65Combined sources3
Beta strandi69 – 75Combined sources7
Beta strandi79 – 84Combined sources6
Beta strandi90 – 93Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AY7X-ray1.70A1-96[»]
1BOXX-ray1.60A1-96[»]
1C54NMR-A1-96[»]
1GMPX-ray1.70A/B1-96[»]
1GMQX-ray1.80A/B1-96[»]
1GMRX-ray1.77A/B1-96[»]
1I70X-ray1.70A/B1-96[»]
1I8VX-ray1.25A/B1-96[»]
1LNIX-ray1.00A/B1-96[»]
1RGEX-ray1.15A/B1-96[»]
1RGFX-ray1.20A/B1-96[»]
1RGGX-ray1.20A/B1-96[»]
1RGHX-ray1.20A/B1-96[»]
1RSNX-ray2.00A/B1-96[»]
1SARX-ray1.80A/B1-96[»]
1T2HX-ray1.00A/B1-96[»]
1T2IX-ray1.10A1-96[»]
1UCIX-ray1.80A/B1-96[»]
1UCJX-ray1.81A/B1-96[»]
1UCKX-ray1.80A/B1-96[»]
1UCLX-ray1.82A/B1-96[»]
1YNVX-ray1.20X1-96[»]
1ZGXX-ray1.13A1-63[»]
B64-96[»]
2SARX-ray1.80A/B1-96[»]
3A5EX-ray1.60A1-96[»]
4GHOX-ray1.10A/B1-96[»]
4J5GX-ray1.31A/B1-94[»]
4J5KX-ray1.23A/B1-96[»]
ProteinModelPortaliP05798.
SMRiP05798.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP05798.

Family & Domainsi

Sequence similaritiesi

Belongs to the ribonuclease N1/T1 family.Curated

Family and domain databases

Gene3Di3.10.450.30. 1 hit.
InterProiIPR000026. Gua-sp_ribonuclease_N1/T1/U2.
IPR016191. Ribonuclease/ribotoxin.
[Graphical view]
PfamiPF00545. Ribonuclease. 1 hit.
[Graphical view]
SUPFAMiSSF53933. SSF53933. 1 hit.

Sequencei

Sequence statusi: Complete.

P05798-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
DVSGTVCLSA LPPEATDTLN LIASDGPFPY SQDGVVFQNR ESVLPTQSYG
60 70 80 90
YYHEYTVITP GARTRGTRRI ITGEATQEDY YTGDHYATFS LIDQTC
Length:96
Mass (Da):10,576
Last modified:April 27, 2001 - v2
Checksum:iFA4DB2FD0BA8A7E4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti72T → C AA sequence (PubMed:3098582).Curated1
Sequence conflicti72T → C AA sequence (PubMed:3118883).Curated1

Sequence databases

PIRiA25655. NRSM.

Cross-referencesi

Sequence databases

PIRiA25655. NRSM.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AY7X-ray1.70A1-96[»]
1BOXX-ray1.60A1-96[»]
1C54NMR-A1-96[»]
1GMPX-ray1.70A/B1-96[»]
1GMQX-ray1.80A/B1-96[»]
1GMRX-ray1.77A/B1-96[»]
1I70X-ray1.70A/B1-96[»]
1I8VX-ray1.25A/B1-96[»]
1LNIX-ray1.00A/B1-96[»]
1RGEX-ray1.15A/B1-96[»]
1RGFX-ray1.20A/B1-96[»]
1RGGX-ray1.20A/B1-96[»]
1RGHX-ray1.20A/B1-96[»]
1RSNX-ray2.00A/B1-96[»]
1SARX-ray1.80A/B1-96[»]
1T2HX-ray1.00A/B1-96[»]
1T2IX-ray1.10A1-96[»]
1UCIX-ray1.80A/B1-96[»]
1UCJX-ray1.81A/B1-96[»]
1UCKX-ray1.80A/B1-96[»]
1UCLX-ray1.82A/B1-96[»]
1YNVX-ray1.20X1-96[»]
1ZGXX-ray1.13A1-63[»]
B64-96[»]
2SARX-ray1.80A/B1-96[»]
3A5EX-ray1.60A1-96[»]
4GHOX-ray1.10A/B1-96[»]
4J5GX-ray1.31A/B1-94[»]
4J5KX-ray1.23A/B1-96[»]
ProteinModelPortaliP05798.
SMRiP05798.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP05798. 1 interactor.
MINTiMINT-1539638.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BRENDAi3.1.27.3. 5978.

Miscellaneous databases

EvolutionaryTraceiP05798.

Family and domain databases

Gene3Di3.10.450.30. 1 hit.
InterProiIPR000026. Gua-sp_ribonuclease_N1/T1/U2.
IPR016191. Ribonuclease/ribotoxin.
[Graphical view]
PfamiPF00545. Ribonuclease. 1 hit.
[Graphical view]
SUPFAMiSSF53933. SSF53933. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRNSA_STRAU
AccessioniPrimary (citable) accession number: P05798
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: April 27, 2001
Last modified: November 2, 2016
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.