P05798 (RNSA_STRAU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Guanyl-specific ribonuclease Sa
Short name=RNase Sa
EC=3.1.27.3

Gene names

Name:

rnaSA

Organism

Streptomyces aureofaciens

Taxonomic identifier

1894 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	96 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Two-stage endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in G-P with 2',3'-cyclic phosphate intermediates.
Subcellular location	Secreted.
Sequence similarities	Belongs to the ribonuclease N1/T1 family.

Ontologies

Keywords
Cellular component	Secreted
Molecular function	Endonuclease Hydrolase Nuclease
PTM	Disulfide bond
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological_process	nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	RNA binding Inferred from electronic annotation. Source: InterPro endoribonuclease activity Inferred from electronic annotation. Source: InterPro ribonuclease T1 activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	P11540	1	EBI-1027193,EBI-1027188	From a different organism.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 96

96

Guanyl-specific ribonuclease Sa

PRO_0000137367

Sites

Active site

54

1

Proton acceptor

Active site

85

1

Proton donor

Amino acid modifications

Disulfide bond

7 ↔ 96

Experimental info

Mutagenesis

39

1

N → A, D or S: Decreases protein stability. Ref.8

Sequence conflict

72

1

T → C AA sequence Ref.1

Sequence conflict

72

1

T → C AA sequence Ref.2

Secondary structure

1

.

.

.

.

.

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.

.

.

.

.

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.

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96

Helix Strand Turn

Details...

Beta strand

5 – 7

3

Helix

8 – 10

3

Helix

13 – 23

11

Turn

31 – 34

4

Beta strand

35 – 37

3

Beta strand

42 – 44

3

Beta strand

48 – 50

3

Beta strand

51 – 56

6

Beta strand

63 – 65

3

Beta strand

69 – 75

7

Beta strand

79 – 84

6

Beta strand

90 – 93

4

Sequences

	Sequence		Length	Mass (Da)
	P05798 [UniParc]. Last modified April 27, 2001. Version 2. Checksum: FA4DB2FD0BA8A7E4	FASTA	96	10,576
	10 20 30 40 50 60 DVSGTVCLSA LPPEATDTLN LIASDGPFPY SQDGVVFQNR ESVLPTQSYG YYHEYTVITP 70 80 90 GARTRGTRRI ITGEATQEDY YTGDHYATFS LIDQTC

References

[1]	"Amino acid sequence determination of guanyl-specific ribonuclease Sa from Streptomyces aureofaciens." Shlyapnikov S.V., Both V., Kulikov V.A., Dementiev A.A., Sevcik J., Zelinka J. FEBS Lett. 209:335-339(1986) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE.
[2]	"Extracellular guanyl-specific ribonuclease Sa from the actinomycete Streptomyces aureofaciens. Primary structure and homology with ribonucleases from bacteria and fungi." Shlyapnikov S.V., Both V., Kulikov V.A., Dementiev A.A., Zelinka J. Bioorg. Khim. 13:760-772(1987) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE.
[3]	"Determination and restrained least-squares refinement of the structures of ribonuclease Sa and its complex with 3'-guanylic acid at 1.8-A resolution." Sevcik J., Dodson E.J., Dodson G.G. Acta Crystallogr. B 47:240-253(1991) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS).
[4]	"Complex of ribonuclease Sa with a cyclic nucleotide and a proposed model for the reaction intermediate." Sevcik J., Zegers I., Wyns L., Dauter Z., Wilson K.S. Eur. J. Biochem. 216:301-305(1993) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
[5]	"Complex of ribonuclease from Streptomyces aureofaciens with 2'-GMP at 1.7-A resolution." Sevcik J., Hill C.P., Dauter Z., Wilson K.S. Acta Crystallogr. D 49:257-271(1993) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS).
[6]	"Ribonuclease from Streptomyces aureofaciens at atomic resolution." Sevcik J., Dauter Z., Lamzin V.S., Wilson K.S. Acta Crystallogr. D 52:327-344(1996) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.15 ANGSTROMS), SEQUENCE REVISION TO 72.
[7]	"Recognition of RNase Sa by the inhibitor barstar: structure of the complex at 1.7 A resolution." Sevcik J., Urbanikova L., Dauter Z., Wilson K.S. Acta Crystallogr. D 54:954-963(1998) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) OF COMPLEX WITH BARSTAR.
[8]	"Contribution of a conserved asparagine to the conformational stability of ribonucleases Sa, Ba, and T1." Hebert E.J., Giletto A., Sevcik J., Urbanikova L., Wilson K.S., Dauter Z., Pace C.N. Biochemistry 37:16192-16200(1998) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS) AND MUTAGENESIS OF ASN-39.
[9]	"Solution structure and dynamics of ribonuclease Sa." Laurents D., Perez-Canadillas J.M., Santoro J., Rico M., Schell D., Pace C.N., Bruix M. Proteins 44:200-211(2001) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR.
+	Additional computationally mapped references.

Cross-references

Sequence databases

PIR

NRSM. A25655.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1AY7	X-ray	1.70	A	1-96	[»]
1BOX	X-ray	1.60	A	1-96	[»]
1C54	NMR	-	A	1-96	[»]
1GMP	X-ray	1.70	A/B	1-96	[»]
1GMQ	X-ray	1.80	A/B	1-96	[»]
1GMR	X-ray	1.77	A/B	1-96	[»]
1I70	X-ray	1.70	A/B	1-96	[»]
1I8V	X-ray	1.25	A/B	1-96	[»]
1LNI	X-ray	1.00	A/B	1-96	[»]
1RGE	X-ray	1.15	A/B	1-96	[»]
1RGF	X-ray	1.20	A/B	1-96	[»]
1RGG	X-ray	1.20	A/B	1-96	[»]
1RGH	X-ray	1.20	A/B	1-96	[»]
1RSN	X-ray	2.00	A/B	1-96	[»]
1SAR	X-ray	1.80	A/B	1-96	[»]
1T2H	X-ray	1.00	A/B	1-96	[»]
1T2I	X-ray	1.10	A	1-96	[»]
1UCI	X-ray	1.80	A/B	3-96	[»]
1UCJ	X-ray	1.81	A/B	1-96	[»]
1UCK	X-ray	1.80	A/B	1-96	[»]
1UCL	X-ray	1.82	A/B	1-96	[»]
1YNV	X-ray	1.20	X	1-96	[»]
1ZGX	X-ray	1.13	A	1-63	[»]
			B	64-96	[»]
2SAR	X-ray	1.80	A/B	1-96	[»]
3A5E	X-ray	1.60	A	2-96	[»]

ProteinModelPortal

P05798.

SMR

P05798. Positions 1-96.

ModBase

Search...

Protein-protein interaction databases

IntAct

P05798. 1 interaction.

MINT

MINT-1539638.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

Gene3D

3.10.450.30. 1 hit.

InterPro

IPR000026. Gua-sp_ribonuclease_N1/T1.
IPR016191. Ribonuclease/ribotoxin.
[Graphical view]

Pfam

PF00545. Ribonuclease. 1 hit.
[Graphical view]

SUPFAM

SSF53933. Ribonuclease/ribotoxin. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

P05798.

Entry information

Entry name

RNSA_STRAU

Accession

Primary (citable) accession number: P05798

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1988
Last sequence update:	April 27, 2001
Last modified:	May 29, 2013
This is version 90 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program