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P05798

- RNSA_STRAU

UniProt

P05798 - RNSA_STRAU

Protein

Guanyl-specific ribonuclease Sa

Gene

rnaSA

Organism
Streptomyces aureofaciens
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Catalytic activityi

    Two-stage endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in G-P with 2',3'-cyclic phosphate intermediates.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei54 – 541Proton acceptor
    Active sitei85 – 851Proton donor

    GO - Molecular functioni

    1. endoribonuclease activity Source: InterPro
    2. ribonuclease T1 activity Source: UniProtKB-EC
    3. RNA binding Source: InterPro

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Guanyl-specific ribonuclease Sa (EC:3.1.27.3)
    Short name:
    RNase Sa
    Gene namesi
    Name:rnaSA
    OrganismiStreptomyces aureofaciens
    Taxonomic identifieri1894 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi39 – 391N → A, D or S: Decreases protein stability.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 9696Guanyl-specific ribonuclease SaPRO_0000137367Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi7 ↔ 96

    Keywords - PTMi

    Disulfide bond

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    P115401EBI-1027193,EBI-1027188From a different organism.

    Protein-protein interaction databases

    IntActiP05798. 1 interaction.
    MINTiMINT-1539638.

    Structurei

    Secondary structure

    1
    96
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi5 – 73
    Helixi8 – 103
    Helixi13 – 2311
    Turni31 – 344
    Beta strandi35 – 373
    Beta strandi42 – 443
    Beta strandi48 – 503
    Beta strandi51 – 566
    Beta strandi63 – 653
    Beta strandi69 – 757
    Beta strandi79 – 846
    Beta strandi90 – 934

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1AY7X-ray1.70A1-96[»]
    1BOXX-ray1.60A1-96[»]
    1C54NMR-A1-96[»]
    1GMPX-ray1.70A/B1-96[»]
    1GMQX-ray1.80A/B1-96[»]
    1GMRX-ray1.77A/B1-96[»]
    1I70X-ray1.70A/B1-96[»]
    1I8VX-ray1.25A/B1-96[»]
    1LNIX-ray1.00A/B1-96[»]
    1RGEX-ray1.15A/B1-96[»]
    1RGFX-ray1.20A/B1-96[»]
    1RGGX-ray1.20A/B1-96[»]
    1RGHX-ray1.20A/B1-96[»]
    1RSNX-ray2.00A/B1-96[»]
    1SARX-ray1.80A/B1-96[»]
    1T2HX-ray1.00A/B1-96[»]
    1T2IX-ray1.10A1-96[»]
    1UCIX-ray1.80A/B1-96[»]
    1UCJX-ray1.81A/B1-96[»]
    1UCKX-ray1.80A/B1-96[»]
    1UCLX-ray1.82A/B1-96[»]
    1YNVX-ray1.20X1-96[»]
    1ZGXX-ray1.13A1-63[»]
    B64-96[»]
    2SARX-ray1.80A/B1-96[»]
    3A5EX-ray1.60A1-96[»]
    4GHOX-ray1.10A/B1-96[»]
    4J5GX-ray1.31A/B1-94[»]
    4J5KX-ray1.23A/B1-96[»]
    ProteinModelPortaliP05798.
    SMRiP05798. Positions 1-96.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP05798.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ribonuclease N1/T1 family.Curated

    Family and domain databases

    Gene3Di3.10.450.30. 1 hit.
    InterProiIPR000026. Gua-sp_ribonuclease_N1/T1.
    IPR016191. Ribonuclease/ribotoxin.
    [Graphical view]
    PfamiPF00545. Ribonuclease. 1 hit.
    [Graphical view]
    SUPFAMiSSF53933. SSF53933. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P05798-1 [UniParc]FASTAAdd to Basket

    « Hide

    DVSGTVCLSA LPPEATDTLN LIASDGPFPY SQDGVVFQNR ESVLPTQSYG   50
    YYHEYTVITP GARTRGTRRI ITGEATQEDY YTGDHYATFS LIDQTC 96
    Length:96
    Mass (Da):10,576
    Last modified:April 27, 2001 - v2
    Checksum:iFA4DB2FD0BA8A7E4
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti72 – 721T → C AA sequence (PubMed:3098582)Curated
    Sequence conflicti72 – 721T → C AA sequence (PubMed:3118883)Curated

    Sequence databases

    PIRiA25655. NRSM.

    Cross-referencesi

    Sequence databases

    PIRi A25655. NRSM.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1AY7 X-ray 1.70 A 1-96 [» ]
    1BOX X-ray 1.60 A 1-96 [» ]
    1C54 NMR - A 1-96 [» ]
    1GMP X-ray 1.70 A/B 1-96 [» ]
    1GMQ X-ray 1.80 A/B 1-96 [» ]
    1GMR X-ray 1.77 A/B 1-96 [» ]
    1I70 X-ray 1.70 A/B 1-96 [» ]
    1I8V X-ray 1.25 A/B 1-96 [» ]
    1LNI X-ray 1.00 A/B 1-96 [» ]
    1RGE X-ray 1.15 A/B 1-96 [» ]
    1RGF X-ray 1.20 A/B 1-96 [» ]
    1RGG X-ray 1.20 A/B 1-96 [» ]
    1RGH X-ray 1.20 A/B 1-96 [» ]
    1RSN X-ray 2.00 A/B 1-96 [» ]
    1SAR X-ray 1.80 A/B 1-96 [» ]
    1T2H X-ray 1.00 A/B 1-96 [» ]
    1T2I X-ray 1.10 A 1-96 [» ]
    1UCI X-ray 1.80 A/B 1-96 [» ]
    1UCJ X-ray 1.81 A/B 1-96 [» ]
    1UCK X-ray 1.80 A/B 1-96 [» ]
    1UCL X-ray 1.82 A/B 1-96 [» ]
    1YNV X-ray 1.20 X 1-96 [» ]
    1ZGX X-ray 1.13 A 1-63 [» ]
    B 64-96 [» ]
    2SAR X-ray 1.80 A/B 1-96 [» ]
    3A5E X-ray 1.60 A 1-96 [» ]
    4GHO X-ray 1.10 A/B 1-96 [» ]
    4J5G X-ray 1.31 A/B 1-94 [» ]
    4J5K X-ray 1.23 A/B 1-96 [» ]
    ProteinModelPortali P05798.
    SMRi P05798. Positions 1-96.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P05798. 1 interaction.
    MINTi MINT-1539638.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P05798.

    Family and domain databases

    Gene3Di 3.10.450.30. 1 hit.
    InterProi IPR000026. Gua-sp_ribonuclease_N1/T1.
    IPR016191. Ribonuclease/ribotoxin.
    [Graphical view ]
    Pfami PF00545. Ribonuclease. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53933. SSF53933. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Amino acid sequence determination of guanyl-specific ribonuclease Sa from Streptomyces aureofaciens."
      Shlyapnikov S.V., Both V., Kulikov V.A., Dementiev A.A., Sevcik J., Zelinka J.
      FEBS Lett. 209:335-339(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE.
    2. "Extracellular guanyl-specific ribonuclease Sa from the actinomycete Streptomyces aureofaciens. Primary structure and homology with ribonucleases from bacteria and fungi."
      Shlyapnikov S.V., Both V., Kulikov V.A., Dementiev A.A., Zelinka J.
      Bioorg. Khim. 13:760-772(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE.
    3. "Determination and restrained least-squares refinement of the structures of ribonuclease Sa and its complex with 3'-guanylic acid at 1.8-A resolution."
      Sevcik J., Dodson E.J., Dodson G.G.
      Acta Crystallogr. B 47:240-253(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS).
    4. "Complex of ribonuclease Sa with a cyclic nucleotide and a proposed model for the reaction intermediate."
      Sevcik J., Zegers I., Wyns L., Dauter Z., Wilson K.S.
      Eur. J. Biochem. 216:301-305(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
    5. "Complex of ribonuclease from Streptomyces aureofaciens with 2'-GMP at 1.7-A resolution."
      Sevcik J., Hill C.P., Dauter Z., Wilson K.S.
      Acta Crystallogr. D 49:257-271(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS).
    6. "Ribonuclease from Streptomyces aureofaciens at atomic resolution."
      Sevcik J., Dauter Z., Lamzin V.S., Wilson K.S.
      Acta Crystallogr. D 52:327-344(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.15 ANGSTROMS), SEQUENCE REVISION TO 72.
    7. "Recognition of RNase Sa by the inhibitor barstar: structure of the complex at 1.7 A resolution."
      Sevcik J., Urbanikova L., Dauter Z., Wilson K.S.
      Acta Crystallogr. D 54:954-963(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) OF COMPLEX WITH BARSTAR.
    8. "Contribution of a conserved asparagine to the conformational stability of ribonucleases Sa, Ba, and T1."
      Hebert E.J., Giletto A., Sevcik J., Urbanikova L., Wilson K.S., Dauter Z., Pace C.N.
      Biochemistry 37:16192-16200(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS) AND MUTAGENESIS OF ASN-39.
    9. Cited for: STRUCTURE BY NMR.

    Entry informationi

    Entry nameiRNSA_STRAU
    AccessioniPrimary (citable) accession number: P05798
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1988
    Last sequence update: April 27, 2001
    Last modified: October 1, 2014
    This is version 96 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3