Reviewed, UniProtKB/Swiss-Prot P05794 (DYR23_ECOLX)

Names and origin

Protein names	Recommended name:     Dihydrofolate reductase type 2     EC=1.5.1.3 Alternative name(s):     Dihydrofolate reductase type II
Encoded on	Plasmid IncP-beta R751
Organism	Escherichia coli
Taxonomic identifier	562 [NCBI]
Taxonomic lineage	Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	78 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Predicted.

General annotation (Comments)

Catalytic activity	5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.
Pathway	Cofactor biosynthesis; tetrahydrofolate biosynthesis; tetrahydrofolate from dihydrofolate: step 1/1.
Miscellaneous	Type II plasmid-specified enzyme is practically insensitive to trimethoprim and methotrexate. The reaction catalyzed by this enzyme represents an essential step for de novo glycine and purine synthesis, DNA precursor synthesis, and for the conversion of dUMP to dTMP.

Ontologies

Keywords
Biological process	Antibiotic resistance Methotrexate resistance One-carbon metabolism Trimethoprim resistance
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Plasmid
Gene Ontology (GO)
Biological process	one-carbon compound metabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW photosynthesis Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW response to drug Inferred from electronic annotation. Source: InterPro response to methotrexate Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	plastid Inferred from electronic annotation. Source: InterPro
Molecular function	dihydrofolate reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 78	78	Dihydrofolate reductase type 2		PRO_0000186437

Sequences

	Sequence		Length	Mass (Da)
	P05794-1 [UniParc]. Last modified November 1, 1988. Version 1. Checksum: 3D67C2EB67220697	FASTA	78	8,328
	10 20 30 40 50 60 MDQHNNGVST LVAGQFALPS HATFGLGDRV RKKSGAAWQG QVVGWYCTKL TPEGYAVESE 70 SHPGSVQIYP VAALERVA

References

[1]	"Nucleotide sequence analysis of the trimethoprim resistant dihydrofolate reductase encoded by R plasmid R751." Flensburg J., Steen R. Nucleic Acids Res. 14:5933-5933(1986) [PubMed: 3526286] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	Radstroem P., Sundstroem L., Swedberg G., Flensburg J., Skoeld O. Submitted (MAR-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	Thomas C.M. Submitted (AUG-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	X04128 Genomic DNA. Translation: CAA27740.1. X72585 Genomic DNA. Translation: CAA51181.1. U67194 Genomic DNA. Translation: AAC64461.1.
PIR	RDECD5. A23598. S32183.
3D structure databases
HSSP	HSSP built from PDB template 1VIE based on UniProtKB P00383.
SMR	P05794. Positions 19-77.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.5.1.3. 246.
Family and domain databases
InterPro	IPR009159. Dhfr_type_II. [Graphical view]
Pfam	PF06442. DHFR_2. 1 hit. [Graphical view]
PIRSF	PIRSF000199. Dhfr_type_II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DYR23_ECOLX

Accession

Primary (citable) accession number: P05794

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1988
Last sequence update:	November 1, 1988
Last modified:	June 16, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)