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Reviewed, UniProtKB/Swiss-Prot P05791 (ILVD_ECOLI)

Last modified November 3, 2009. Version 106. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Dihydroxy-acid dehydratase
      Short name=DAD
    EC=4.2.1.9
Gene names
Name: ilvD
Ordered Locus Names: b3771, JW5605
OrganismEscherichia coli (strain K12) [Complete proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

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Protein attributes

Sequence length616 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Catalytic activity

2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012

Cofactor

Binds 1 4Fe-4S cluster. HAMAP MF_00012

Pathway

Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012

Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012

Subunit structure

Homodimer. Ref.9

Sequence similarities

Belongs to the ilvD/edd family.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.9
Chain2 – 616615Dihydroxy-acid dehydratase HAMAP MF_00012
PRO_0000103465

Sites

Metal binding1221Iron-sulfur (4Fe-4S) Potential
Metal binding1951Iron-sulfur (4Fe-4S) Potential

Experimental info

Sequence conflict2 – 2423PKYRS…ALWRA → VVIWRVLVRCGP in AAA24023. Ref.2
Sequence conflict1641L → P in AAA24023. Ref.2
Sequence conflict408 – 43730CIRSL…GCIVK → VSARWNTPTAKTAAWRCSTV ILRKRLHRE in AAA24023. Ref.2
Sequence conflict6101D → H in AAA24023. Ref.2

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Sequences

Sequence LengthMass (Da)Tools
P05791-1 [UniParc].

Last modified January 23, 2007. Version 5.
Checksum: 6F6DA053CFDA475B

FASTA61665,532
        10         20         30         40         50         60 
MPKYRSATTT HGRNMAGARA LWRATGMTDA DFGKPIIAVV NSFTQFVPGH VHLRDLGKLV 

        70         80         90        100        110        120 
AEQIEAAGGV AKEFNTIAVD DGIAMGHGGM LYSLPSRELI ADSVEYMVNA HCADAMVCIS 

       130        140        150        160        170        180 
NCDKITPGML MASLRLNIPV IFVSGGPMEA GKTKLSDQII KLDLVDAMIQ GADPKVSDSQ 

       190        200        210        220        230        240 
SDQVERSACP TCGSCSGMFT ANSMNCLTEA LGLSQPGNGS LLATHADRKQ LFLNAGKRIV 

       250        260        270        280        290        300 
ELTKRYYEQN DESALPRNIA SKAAFENAMT LDIAMGGSTN TVLHLLAAAQ EAEIDFTMSD 

       310        320        330        340        350        360 
IDKLSRKVPQ LCKVAPSTQK YHMEDVHRAG GVIGILGELD RAGLLNRDVK NVLGLTLPQT 

       370        380        390        400        410        420 
LEQYDVMLTQ DDAVKNMFRA GPAGIRTTQA FSQDCRWDTL DDDRANGCIR SLEHAYSKDG 

       430        440        450        460        470        480 
GLAVLYGNFA ENGCIVKTAG VDDSILKFTG PAKVYESQDD AVEAILGGKV VAGDVVVIRY 

       490        500        510        520        530        540 
EGPKGGPGMQ EMLYPTSFLK SMGLGKACAL ITDGRFSGGT SGLSIGHVSP EAASGGSIGL 

       550        560        570        580        590        600 
IEDGDLIAID IPNRGIQLQV SDAELAARRE AQDARGDKAW TPKNRERQVS FALRAYASLA 

       610 
TSADKGAVRD KSKLGG

« Hide

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References

« Hide 'large scale' references
[1]"The complete nucleotide sequence of the ilvGMEDA cluster of Escherichia coli K-12."
Cox J.L., Cox B.J., Fidanza V., Calhoun D.H.
Gene 56:185-198(1987) [PubMed: 3315862] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[2]"The complete nucleotide sequence of the ilvGMEDA operon of Escherichia coli K-12."
Lawther R.P., Wek R.C., Lopes J.M., Pereira R., Taillon B.E., Hatfield G.W.
Nucleic Acids Res. 15:2137-2155(1987) [PubMed: 3550695] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[3]"Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes."
Daniels D.L., Plunkett G. III, Burland V.D., Blattner F.R.
Science 257:771-778(1992) [PubMed: 1379743] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[5]"Escherichia coli K-12: a cooperatively developed annotation snapshot -- 2005."
Riley M., Abe T., Arnaud M.B., Berlyn M.K.B., Blattner F.R., Chaudhuri R.R., Glasner J.D., Horiuchi T., Keseler I.M., Kosuge T., Mori H., Perna N.T., Plunkett G. III, Rudd K.E., Serres M.H., Thomas G.H., Thomson N.R., Wishart D., Wanner B.L.
Nucleic Acids Res. 34:1-9(2006) [PubMed: 16397293] [Abstract]
Cited for: SEQUENCE REVISION.
[6]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[7]"Branched-chain amino acid aminotransferase of Escherichia coli: nucleotide sequence of the ilvE gene and the deduced amino acid sequence."
Kuramitsu S., Ogawa T., Ogawa H., Kagamiyama H.
J. Biochem. 97:993-999(1985) [PubMed: 3897211] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-100.
Strain: K12.
[8]Garrison E., Harms E.H., Umbarger H.E.
Submitted (JUN-1987) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 575-616.
[9]"The role and properties of the iron-sulfur cluster in Escherichia coli dihydroxy-acid dehydratase."
Flint D.H., Emptage M.H., Finnegan M.G., Fu W., Johnson M.K.
J. Biol. Chem. 268:14732-14742(1993) [PubMed: 8325851] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-23, PRESENCE OF A 4FE--4S IRON-SULFUR CLUSTER, SUBUNIT.
Strain: K12 / JA221 / ATCC 33875 / DSM 5209.
[10]"Dihydroxy-acid dehydratase, a 4Fe-4S cluster-containing enzyme in Escherichia coli: effects of intracellular superoxide dismutase on its inactivation by oxidant stress."
Brown O.R., Smyk-Randall E., Draczynska-Lusiak B., Fee J.A.
Arch. Biochem. Biophys. 319:10-22(1995) [PubMed: 7771772] [Abstract]
Cited for: PRESENCE OF A 4FE--4S IRON-SULFUR CLUSTER.

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Cross-references

Sequence databases

X04890 Genomic DNA. Translation: CAA28576.1.
M10313 Genomic DNA. Translation: AAB59053.1.
M87049 Genomic DNA. Translation: AAA67574.1.
U00096 Genomic DNA. Translation: AAT48208.1.
AP009048 Genomic DNA. Translation: BAE77526.1.
M32253 Genomic DNA. Translation: AAA24023.1.
X02413 Genomic DNA. Translation: CAA26263.1.
K03503 Genomic DNA. Translation: AAA24013.1.
PIRDWECDA. A27310.
RefSeqAP_004025.1.
YP_026248.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

DIPDIP:10021N.
STRINGP05791.

2-D gel databases

SWISS-2DPAGEP05791.

Genome annotation databases

GeneID948277.
GenomeReviewsGene locus JW5605 in contig AP009048_GR.
Gene locus b3771 in contig U00096_GR.
KEGGecj:JW5605.
eco:b3771.

Organism-specific databases

EchoBASEEB0491.
EcoGeneEG10496. ilvD.
CMRSearch...

Phylogenomic databases

HOGENOMP05791.
OMASRKVPCL.

Enzyme and pathway databases

BioCycEcoCyc:DIHYDROXYACIDDEHYDRAT-MON.
MetaCyc:DIHYDROXYACIDDEHYDRAT-MON.

Gene expression databases

GenevestigatorP05791.

Family and domain databases

HAMAPMF_00012.
[Tree]
InterProIPR004404. DihydroxyA_deHydtase.
IPR000581. DiOHA_6PGluconate_deHydtase.
IPR020558. DiOHA_6PGluconate_deHydtase_CS.
[Graphical view]
PANTHERPTHR21000. ILVD_EDD_family. 1 hit.
PfamPF00920. ILVD_EDD. 1 hit.
[Graphical view]
ProDomPD002691. ILVD_EDD_family. 1 hit.
[Graphical view] [Entries sharing at least one domain]
TIGRFAMsTIGR00110. ilvD. 1 hit.
PROSITEPS00886. ILVD_EDD_1. 1 hit.
PS00887. ILVD_EDD_2. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameILVD_ECOLI
AccessionPrimary (citable) accession number: P05791
Secondary accession number(s): Q2M880
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: January 23, 2007
Last modified: November 3, 2009
This is version 106 of the entry and version 5 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents