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Protein

Dihydroxy-acid dehydratase

Gene

ilvD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O.UniRule annotation2 Publications

Cofactori

[4Fe-4S] clusterUniRule annotation2 PublicationsNote: Binds 1 [4Fe-4S] cluster.UniRule annotation2 Publications

Pathwayi: L-isoleucine biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes L-isoleucine from 2-oxobutanoate.UniRule annotation1 Publication
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Acetolactate synthase isozyme 1 small subunit (ilvN), Acetolactate synthase isozyme 1 large subunit (ilvB), Putative acetolactate synthase isozyme 2 large subunit (ilvG), Acetolactate synthase isozyme 2 large subunit (ilvG), Acetolactate synthase isozyme 2 small subunit (ilvM), Acetolactate synthase isozyme 3 large subunit (ilvI), Acetolactate synthase isozyme 3 small subunit (ilvH)
  2. Ketol-acid reductoisomerase (NADP(+)) (ilvC)
  3. Dihydroxy-acid dehydratase (ilvD)
  4. Branched-chain-amino-acid aminotransferase (ilvE)
This subpathway is part of the pathway L-isoleucine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-isoleucine from 2-oxobutanoate, the pathway L-isoleucine biosynthesis and in Amino-acid biosynthesis.

Pathwayi: L-valine biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes L-valine from pyruvate.UniRule annotation1 Publication
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Acetolactate synthase isozyme 1 small subunit (ilvN), Acetolactate synthase isozyme 1 large subunit (ilvB), Putative acetolactate synthase isozyme 2 large subunit (ilvG), Acetolactate synthase isozyme 2 large subunit (ilvG), Acetolactate synthase isozyme 2 small subunit (ilvM), Acetolactate synthase isozyme 3 large subunit (ilvI), Acetolactate synthase isozyme 3 small subunit (ilvH)
  2. Ketol-acid reductoisomerase (NADP(+)) (ilvC)
  3. Dihydroxy-acid dehydratase (ilvD)
  4. Branched-chain-amino-acid aminotransferase (ilvE)
This subpathway is part of the pathway L-valine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-valine from pyruvate, the pathway L-valine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi122Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi195Iron-sulfur (4Fe-4S)UniRule annotation1

GO - Molecular functioni

  • 4 iron, 4 sulfur cluster binding Source: EcoCyc
  • dihydroxy-acid dehydratase activity Source: EcoCyc
  • iron-sulfur cluster binding Source: EcoCyc
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • isoleucine biosynthetic process Source: EcoCyc
  • valine biosynthetic process Source: EcoCyc

Keywordsi

Molecular functionLyase
Biological processAmino-acid biosynthesis, Branched-chain amino acid biosynthesis
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:DIHYDROXYACIDDEHYDRAT-MONOMER
MetaCyc:DIHYDROXYACIDDEHYDRAT-MONOMER
UniPathwayiUPA00047; UER00057
UPA00049; UER00061

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydroxy-acid dehydrataseUniRule annotation (EC:4.2.1.9UniRule annotation)
Short name:
DADUniRule annotation
Gene namesi
Name:ilvDUniRule annotation
Ordered Locus Names:b3771, JW5605
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10496 ilvD

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001034652 – 616Dihydroxy-acid dehydrataseAdd BLAST615

Proteomic databases

EPDiP05791
PaxDbiP05791
PRIDEiP05791

2D gel databases

SWISS-2DPAGEiP05791

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi4263329, 5 interactors
DIPiDIP-10021N
IntActiP05791, 1 interactor
STRINGi316407.85676276

Structurei

3D structure databases

ProteinModelPortaliP05791
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the IlvD/Edd family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C01 Bacteria
COG0129 LUCA
HOGENOMiHOG000173155
InParanoidiP05791
KOiK01687
OMAiIPGHVHL
PhylomeDBiP05791

Family and domain databases

HAMAPiMF_00012 IlvD, 1 hit
InterProiView protein in InterPro
IPR004404 DihydroxyA_deHydtase
IPR000581 DiOHA_6PGluconate_deHydtase
IPR020558 DiOHA_6PGluconate_deHydtase_CS
IPR037237 IlvD/EDD_N
PfamiView protein in Pfam
PF00920 ILVD_EDD, 1 hit
SUPFAMiSSF143975 SSF143975, 1 hit
TIGRFAMsiTIGR00110 ilvD, 1 hit
PROSITEiView protein in PROSITE
PS00886 ILVD_EDD_1, 1 hit
PS00887 ILVD_EDD_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P05791-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPKYRSATTT HGRNMAGARA LWRATGMTDA DFGKPIIAVV NSFTQFVPGH
60 70 80 90 100
VHLRDLGKLV AEQIEAAGGV AKEFNTIAVD DGIAMGHGGM LYSLPSRELI
110 120 130 140 150
ADSVEYMVNA HCADAMVCIS NCDKITPGML MASLRLNIPV IFVSGGPMEA
160 170 180 190 200
GKTKLSDQII KLDLVDAMIQ GADPKVSDSQ SDQVERSACP TCGSCSGMFT
210 220 230 240 250
ANSMNCLTEA LGLSQPGNGS LLATHADRKQ LFLNAGKRIV ELTKRYYEQN
260 270 280 290 300
DESALPRNIA SKAAFENAMT LDIAMGGSTN TVLHLLAAAQ EAEIDFTMSD
310 320 330 340 350
IDKLSRKVPQ LCKVAPSTQK YHMEDVHRAG GVIGILGELD RAGLLNRDVK
360 370 380 390 400
NVLGLTLPQT LEQYDVMLTQ DDAVKNMFRA GPAGIRTTQA FSQDCRWDTL
410 420 430 440 450
DDDRANGCIR SLEHAYSKDG GLAVLYGNFA ENGCIVKTAG VDDSILKFTG
460 470 480 490 500
PAKVYESQDD AVEAILGGKV VAGDVVVIRY EGPKGGPGMQ EMLYPTSFLK
510 520 530 540 550
SMGLGKACAL ITDGRFSGGT SGLSIGHVSP EAASGGSIGL IEDGDLIAID
560 570 580 590 600
IPNRGIQLQV SDAELAARRE AQDARGDKAW TPKNRERQVS FALRAYASLA
610
TSADKGAVRD KSKLGG
Length:616
Mass (Da):65,532
Last modified:January 23, 2007 - v5
Checksum:i6F6DA053CFDA475B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti2 – 24PKYRS…ALWRA → VVIWRVLVRCGP in AAA24023 (PubMed:3550695).CuratedAdd BLAST23
Sequence conflicti164L → P in AAA24023 (PubMed:3550695).Curated1
Sequence conflicti408 – 437CIRSL…GCIVK → VSARWNTPTAKTAAWRCSTV ILRKRLHRE in AAA24023 (PubMed:3550695).CuratedAdd BLAST30
Sequence conflicti610D → H in AAA24023 (PubMed:3550695).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04890 Genomic DNA Translation: CAA28576.1
M10313 Genomic DNA Translation: AAB59053.1
M87049 Genomic DNA Translation: AAA67574.1
U00096 Genomic DNA Translation: AAT48208.1
AP009048 Genomic DNA Translation: BAE77526.1
M32253 Genomic DNA Translation: AAA24023.1
X02413 Genomic DNA Translation: CAA26263.1
K03503 Genomic DNA Translation: AAA24013.1
PIRiA27310 DWECDA
RefSeqiWP_001127399.1, NZ_LN832404.1
YP_026248.1, NC_000913.3

Genome annotation databases

EnsemblBacteriaiAAT48208; AAT48208; b3771
BAE77526; BAE77526; BAE77526
GeneIDi948277
KEGGiecj:JW5605
eco:b3771
PATRICifig|1411691.4.peg.2935

Similar proteinsi

Entry informationi

Entry nameiILVD_ECOLI
AccessioniPrimary (citable) accession number: P05791
Secondary accession number(s): Q2M880
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: January 23, 2007
Last modified: April 25, 2018
This is version 172 of the entry and version 5 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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