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P05777

- M1_I33A0

UniProt

P05777 - M1_I33A0

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Protein

Matrix protein 1

Gene

M

Organism
Influenza A virus (strain A/Wilson-Smith/1933 H1N1) (Influenza A virus (strain A/WS/1933 H1N1))
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Plays critical roles in virus replication, from virus entry and uncoating to assembly and budding of the virus particle. M1 binding to ribonucleocapsids (RNPs) in nucleus seems to inhibit viral transcription. Interaction of viral NEP with M1-RNP is thought to promote nuclear export of the complex, which is targeted to the virion assembly site at the apical plasma membrane in polarized epithelial cells. Interactions with NA and HA may bring M1, a non-raft-associated protein, into lipid rafts. Forms a continuous shell on the inner side of the lipid bilayer in virion, where it binds the RNP. During virus entry into cell, the M2 ion channel acidifies the internal virion core, inducing M1 dissociation from the RNP. M1-free RNPs are transported to the nucleus, where viral transcription and replication can take place.1 Publication
Determines the virion's shape: spherical or filamentous. Clinical isolates of influenza are characterized by the presence of significant proportion of filamentous virions, whereas after multiple passage on eggs or cell culture, virions have only spherical morphology. Filamentous virions are thought to be important to infect neighboring cells, and spherical virions more suited to spread through aerosol between hosts organisms.1 Publication

GO - Molecular functioni

  1. RNA binding Source: UniProtKB-KW
  2. structural constituent of virion Source: UniProtKB-KW

GO - Biological processi

  1. suppression by virus of host complement activation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Host-virus interaction, Inhibition of host complement factors by virus, Viral immunoevasion

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Matrix protein 1
Short name:
M1
Gene namesi
Name:M
OrganismiInfluenza A virus (strain A/Wilson-Smith/1933 H1N1) (Influenza A virus (strain A/WS/1933 H1N1))
Taxonomic identifieri381518 [NCBI]
Taxonomic lineageiVirusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus A
Virus hostiAves [TaxID: 8782]
Homo sapiens (Human) [TaxID: 9606]
Sus scrofa (Pig) [TaxID: 9823]
ProteomesiUP000000834: Genome

Subcellular locationi

GO - Cellular componenti

  1. host cell nucleus Source: UniProtKB-KW
  2. membrane Source: UniProtKB-KW
  3. virion Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host nucleus, Membrane, Viral matrix protein, Virion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi41 – 411V → A: Induces short filamentous virions. 1 Publication
Mutagenesisi95 – 951K → A or R: No effect. 2 Publications
Mutagenesisi97 – 971V → A: No effect. 1 Publication
Mutagenesisi98 – 981K → A or R: No effect. 1 Publication
Mutagenesisi99 – 991L → A: No effect. 1 Publication
Mutagenesisi100 – 1034YRKL → AAAA or PPPY: Can't be rescued by reverse genetic.
Mutagenesisi100 – 1034YRKL → PTAP: No effect.
Mutagenesisi100 – 1001Y → A, F or S: No effect. 1 Publication
Mutagenesisi101 – 1055RKLKR → SNLNS: 50% loss of RNA binding activity. 1 Publication
Mutagenesisi101 – 1022RK → PD: No effect.
Mutagenesisi101 – 1011R → A: Reduced growth and small-plaque morphology. Virions have an elongated filamentous morphology. 1 Publication
Mutagenesisi101 – 1011R → K: No effect. 1 Publication
Mutagenesisi102 – 1021K → A or R: No effect. 1 Publication
Mutagenesisi103 – 1031L → A, I or V: No effect. 1 Publication
Mutagenesisi104 – 1041K → A: Can't be rescued by reverse genetic. 1 Publication
Mutagenesisi104 – 1041K → R: No effect. 1 Publication
Mutagenesisi105 – 1051R → A: Can't be rescued by reverse genetic. 1 Publication
Mutagenesisi105 – 1051R → K: Reduced growth and small-plaque morphology. 1 Publication
Mutagenesisi106 – 1061E → A: Can't be rescued by reverse genetic. 1 Publication
Mutagenesisi148 – 1481C → A: No effect. 2 Publications
Mutagenesisi148 – 1481C → S: 31% loss of RNA binding activity. 2 Publications
Mutagenesisi151 – 1511C → A: No effect. 1 Publication
Mutagenesisi155 – 1551A → G: Complete loss of virus ability to be rescued in a reverse genetic system. 1 Publication
Mutagenesisi159 – 1591H → A: No effect. 1 Publication
Mutagenesisi162 – 1621H → A: No effect. 1 Publication
Mutagenesisi218 – 2181T → A: No effect on virion morphology. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 252252Matrix protein 1PRO_0000078868Add
BLAST

Interactioni

Subunit structurei

Homodimer and homomultimer. Interacts with NEP (By similarity). Binds ribonucleocapsid by both interacting with genomic RNA and NP protein. May interact with HA and NA (By similarity). Cannot bind NP without genomic RNA.By similarity1 Publication

Structurei

3D structure databases

ProteinModelPortaliP05777.
SMRiP05777. Positions 1-158.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 164164Membrane-bindingAdd
BLAST
Regioni165 – 25288RNP-bindingAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi101 – 1055Nuclear localization signal

Sequence similaritiesi

Family and domain databases

Gene3Di1.10.10.180. 1 hit.
1.20.91.10. 1 hit.
InterProiIPR013188. Flu_matrix_M1_C.
IPR001561. Flu_matrix_M1_N.
IPR015423. Flu_matrix_M1_N_sub1.
IPR015799. Flu_matrix_M1_N_sub2.
[Graphical view]
PfamiPF00598. Flu_M1. 1 hit.
PF08289. Flu_M1_C. 1 hit.
[Graphical view]
ProDomiPD596253. Flu_matrix_M1_C. 1 hit.
PD001061. Flu_matrix_M1_N. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00759. Flu_M1_C. 1 hit.
[Graphical view]
SUPFAMiSSF48145. SSF48145. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Note: Only the first 9 residues are shared by the 2 isoforms.

Isoform M1 (identifier: P05777-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSLLTEVETY VLSIVPSGPL KAEIAQRLED VFAGKNTDLE VLMEWLKTRP
60 70 80 90 100
ILSPLTKGIL GFVFTLTVPS ERGLQRRRFV QNALNGNGDP NNMDKAVKLY
110 120 130 140 150
RKLKREITFH GAKEIALSYS AGALASCMGL IYNRMGAVTT EVAFGLVCAT
160 170 180 190 200
CEQIADSQHR SHRQMVTTTN PLIRHENRMV LASTTAKAME QMAGSSEQAA
210 220 230 240 250
EAMDIASQAR QMVQAMRTIG THPSSSAGLK DDLLENLQAY QKRMGVQMQR

FK
Length:252
Mass (Da):27,864
Last modified:July 1, 1989 - v2
Checksum:i5F300F18D75BBAD3
GO
Isoform M2 (identifier: P05780-1) [UniParc]FASTAAdd to Basket

The sequence of this isoform can be found in the external entry P05780.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:97
Mass (Da):11,313
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti117 – 1171L → F in AAA43352. (PubMed:3354209)Curated
Sequence conflicti219 – 2191I → V in AAA43352. (PubMed:3354209)Curated
Sequence conflicti231 – 2311D → S in AAA43252. (PubMed:3414185)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X08088 Genomic RNA. Translation: CAA30882.1.
M23920 Genomic RNA. Translation: AAA43252.1. Sequence problems.
M19374 Genomic RNA. Translation: AAA43352.1.
L25818 Genomic RNA. Translation: AAA91325.1.
PIRiA28608. MFIVWS.
S07429.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X08088 Genomic RNA. Translation: CAA30882.1 .
M23920 Genomic RNA. Translation: AAA43252.1 . Sequence problems.
M19374 Genomic RNA. Translation: AAA43352.1 .
L25818 Genomic RNA. Translation: AAA91325.1 .
PIRi A28608. MFIVWS.
S07429.

3D structure databases

ProteinModelPortali P05777.
SMRi P05777. Positions 1-158.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 1.10.10.180. 1 hit.
1.20.91.10. 1 hit.
InterProi IPR013188. Flu_matrix_M1_C.
IPR001561. Flu_matrix_M1_N.
IPR015423. Flu_matrix_M1_N_sub1.
IPR015799. Flu_matrix_M1_N_sub2.
[Graphical view ]
Pfami PF00598. Flu_M1. 1 hit.
PF08289. Flu_M1_C. 1 hit.
[Graphical view ]
ProDomi PD596253. Flu_matrix_M1_C. 1 hit.
PD001061. Flu_matrix_M1_N. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
SMARTi SM00759. Flu_M1_C. 1 hit.
[Graphical view ]
SUPFAMi SSF48145. SSF48145. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequences of influenza A virus RNA segment 7: a comparison of five isolates."
    Zebedee S.L., Lamb R.A.
    Nucleic Acids Res. 17:2870-2870(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Nucleotide sequence of RNA segment 7 and the predicted amino sequence of M1 and M2 proteins of FPV/Weybridge (H7N7) and WSN (H1N1) influenza viruses."
    Markushin S., Ghiasi H., Sokolov N., Shilov A., Sinitsin B., Brown D., Klimov A., Nayak D.
    Virus Res. 10:263-272(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  3. "Transient expression and sequence of the matrix (M1) gene of WSN influenza A virus in a vaccinia vector."
    Baylor N.W., Zhiping Y.L., Wagner R.R.
    Virology 163:618-621(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  4. "Association of influenza virus matrix protein with ribonucleoproteins."
    Ye Z., Liu T., Offringa D.P., McInnis J., Levandowski R.A.
    J. Virol. 73:7467-7473(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF 101-ARG--ARG-105 AND CYS-148.
  5. "Influenza virus assembly: effect of influenza virus glycoproteins on the membrane association of M1 protein."
    Ali A., Avalos R.T., Ponimaskin E., Nayak D.P.
    J. Virol. 74:8709-8719(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH HA AND NA.
  6. "Conserved cysteine and histidine residues in the putative zinc finger motif of the influenza A virus M1 protein are not critical for influenza virus replication."
    Hui E.K., Ralston K., Judd A.K., Nayak D.P.
    J. Gen. Virol. 84:3105-3113(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF CYS-148; CYS-151; ALA-155; HIS-159 AND HIS-162.
  7. "Reverse genetics studies on the filamentous morphology of influenza A virus."
    Bourmakina S.V., Garcia-Sastre A.
    J. Gen. Virol. 84:517-527(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "Basic residues of the helix six domain of influenza virus M1 involved in nuclear translocation of M1 can be replaced by PTAP and YPDL late assembly domain motifs."
    Hui E.K., Barman S., Yang T.Y., Nayak D.P.
    J. Virol. 77:7078-7092(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF LYS-95; VAL-97; LYS-98; LEU-99; TYR-100; ARG-101; LYS-102; LEU-103; LYS-104; ARG-105 AND GLU-106.
  9. "The M1 matrix protein controls the filamentous phenotype of influenza A virus."
    Elleman C.J., Barclay W.S.
    Virology 321:144-153(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF VAL-41; LYS-95 AND THR-218.

Entry informationi

Entry nameiM1_I33A0
AccessioniPrimary (citable) accession number: P05777
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: July 1, 1989
Last modified: October 29, 2014
This is version 90 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

Most abundant protein in virion. When expressed alone can form virus-like particles in transfected cells.

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3