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Protein

Matrix protein 1

Gene

M

Organism
Influenza A virus (strain A/Wilson-Smith/1933 H1N1) (Influenza A virus (strain A/WS/1933 H1N1))
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays critical roles in virus replication, from virus entry and uncoating to assembly and budding of the virus particle. M1 binding to ribonucleocapsids (RNPs) in nucleus seems to inhibit viral transcription. Interaction of viral NEP with M1-RNP is thought to promote nuclear export of the complex, which is targeted to the virion assembly site at the apical plasma membrane in polarized epithelial cells. Interactions with NA and HA may bring M1, a non-raft-associated protein, into lipid rafts. Forms a continuous shell on the inner side of the lipid bilayer in virion, where it binds the RNP. During virus entry into cell, the M2 ion channel acidifies the internal virion core, inducing M1 dissociation from the RNP. M1-free RNPs are transported to the nucleus, where viral transcription and replication can take place.UniRule annotation1 Publication
Determines the virion's shape: spherical or filamentous. Clinical isolates of influenza are characterized by the presence of significant proportion of filamentous virions, whereas after multiple passage on eggs or cell culture, virions have only spherical morphology. Filamentous virions are thought to be important to infect neighboring cells, and spherical virions more suited to spread through aerosol between hosts organisms.UniRule annotation1 Publication

Miscellaneous

Most abundant protein in virion. When expressed alone can form virus-like particles in transfected cells.UniRule annotation

GO - Molecular functioni

Keywordsi

Molecular functionRNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Matrix protein 1UniRule annotation
Short name:
M1UniRule annotation
Gene namesi
Name:MUniRule annotation
OrganismiInfluenza A virus (strain A/Wilson-Smith/1933 H1N1) (Influenza A virus (strain A/WS/1933 H1N1))
Taxonomic identifieri381518 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus A
Virus hostiAves [TaxID: 8782]
Homo sapiens (Human) [TaxID: 9606]
Sus scrofa (Pig) [TaxID: 9823]

Subcellular locationi

  • Virion membrane UniRule annotation; Peripheral membrane protein UniRule annotation; Cytoplasmic side UniRule annotation
  • Host nucleus UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Host nucleus, Membrane, Viral matrix protein, Virion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi41V → A: Induces short filamentous virions. 1 Publication1
Mutagenesisi95K → A or R: No effect. 2 Publications1
Mutagenesisi97V → A: No effect. 1 Publication1
Mutagenesisi98K → A or R: No effect. 1 Publication1
Mutagenesisi99L → A: No effect. 1 Publication1
Mutagenesisi100 – 103YRKL → AAAA or PPPY: Can't be rescued by reverse genetic. 4
Mutagenesisi100 – 103YRKL → PTAP: No effect. 4
Mutagenesisi100Y → A, F or S: No effect. 1 Publication1
Mutagenesisi101 – 105RKLKR → SNLNS: 50% loss of RNA binding activity. 1 Publication5
Mutagenesisi101 – 102RK → PD: No effect. 2
Mutagenesisi101R → A: Reduced growth and small-plaque morphology. Virions have an elongated filamentous morphology. 1 Publication1
Mutagenesisi101R → K: No effect. 1 Publication1
Mutagenesisi102K → A or R: No effect. 1 Publication1
Mutagenesisi103L → A, I or V: No effect. 1 Publication1
Mutagenesisi104K → A: Can't be rescued by reverse genetic. 1 Publication1
Mutagenesisi104K → R: No effect. 1 Publication1
Mutagenesisi105R → A: Can't be rescued by reverse genetic. 1 Publication1
Mutagenesisi105R → K: Reduced growth and small-plaque morphology. 1 Publication1
Mutagenesisi106E → A: Can't be rescued by reverse genetic. 1 Publication1
Mutagenesisi148C → A: No effect. 2 Publications1
Mutagenesisi148C → S: 31% loss of RNA binding activity. 2 Publications1
Mutagenesisi151C → A: No effect. 1 Publication1
Mutagenesisi155A → G: Complete loss of virus ability to be rescued in a reverse genetic system. 1 Publication1
Mutagenesisi159H → A: No effect. 1 Publication1
Mutagenesisi162H → A: No effect. 1 Publication1
Mutagenesisi218T → A: No effect on virion morphology. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000788681 – 252Matrix protein 1Add BLAST252

Interactioni

Subunit structurei

Homodimer and homomultimer. Interacts with NEP. Binds ribonucleocapsid by both interacting with genomic RNA and NP protein. May interact with HA and NA. Cannot bind NP without genomic RNA.UniRule annotation1 Publication

Structurei

Secondary structure

1252
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 12Combined sources10
Helixi19 – 32Combined sources14
Helixi39 – 48Combined sources10
Helixi54 – 67Combined sources14
Helixi78 – 83Combined sources6
Helixi90 – 104Combined sources15
Helixi109 – 116Combined sources8
Helixi121 – 132Combined sources12
Helixi133 – 137Combined sources5
Helixi140 – 157Combined sources18

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4PUSX-ray2.20A/B2-165[»]
5V6GX-ray2.00A/B/C/D2-165[»]
5V7BX-ray2.50A/B2-165[»]
5V7SX-ray2.50A/B/C2-165[»]
5V8AX-ray3.00A2-165[»]
ProteinModelPortaliP05777.
SMRiP05777.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 164Membrane-bindingUniRule annotationAdd BLAST164
Regioni165 – 252RNP-bindingUniRule annotationAdd BLAST88

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi101 – 105Nuclear localization signalUniRule annotation5

Sequence similaritiesi

Belongs to the influenza viruses Matrix protein M1 family.UniRule annotation

Phylogenomic databases

OrthoDBiVOG090000KZ.

Family and domain databases

Gene3Di1.10.10.180. 1 hit.
HAMAPiMF_04068. INFV_M1. 1 hit.
InterProiView protein in InterPro
IPR013188. Flu_matrix_M1_C.
IPR001561. Flu_matrix_M1_N.
IPR015799. Flu_matrix_M1_N_sub2.
PfamiView protein in Pfam
PF00598. Flu_M1. 1 hit.
PF08289. Flu_M1_C. 1 hit.
ProDomiView protein in ProDom or Entries sharing at least one domain
PD596253. Flu_matrix_M1_C. 1 hit.
PD001061. Flu_matrix_M1_N. 1 hit.
SMARTiView protein in SMART
SM00759. Flu_M1_C. 1 hit.
SUPFAMiSSF48145. SSF48145. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Only the first 9 residues are shared by the 2 isoforms.
Isoform M1 (identifier: P05777-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSLLTEVETY VLSIVPSGPL KAEIAQRLED VFAGKNTDLE VLMEWLKTRP
60 70 80 90 100
ILSPLTKGIL GFVFTLTVPS ERGLQRRRFV QNALNGNGDP NNMDKAVKLY
110 120 130 140 150
RKLKREITFH GAKEIALSYS AGALASCMGL IYNRMGAVTT EVAFGLVCAT
160 170 180 190 200
CEQIADSQHR SHRQMVTTTN PLIRHENRMV LASTTAKAME QMAGSSEQAA
210 220 230 240 250
EAMDIASQAR QMVQAMRTIG THPSSSAGLK DDLLENLQAY QKRMGVQMQR

FK
Length:252
Mass (Da):27,864
Last modified:July 1, 1989 - v2
Checksum:i5F300F18D75BBAD3
GO
Isoform M2 (identifier: P05780-1) [UniParc]FASTAAdd to basket
The sequence of this isoform can be found in the external entry P05780.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:97
Mass (Da):11,313
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti117L → F in AAA43352 (PubMed:3354209).1
Sequence conflicti219I → V in AAA43352 (PubMed:3354209).1
Sequence conflicti231D → S in AAA43252 (PubMed:3414185).1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X08088 Genomic RNA. Translation: CAA30882.1.
M23920 Genomic RNA. Translation: AAA43252.1. Sequence problems.
M19374 Genomic RNA. Translation: AAA43352.1.
L25818 Genomic RNA. Translation: AAA91325.1.
PIRiA28608. MFIVWS.
S07429.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiM1_I33A0
AccessioniPrimary (citable) accession number: P05777
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: July 1, 1989
Last modified: August 30, 2017
This is version 103 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families