Reviewed, UniProtKB/Swiss-Prot P05772 (KPCB_RABIT)

Names and origin

Protein names

Recommended name:
    Protein kinase C beta type
      Short name=PKC-beta
      Short name=PKC-B
    EC=2.7.11.13

Gene names

Name:	PRKCB
Synonyms:	PRKCB1

Organism

Oryctolagus cuniculus (Rabbit)

Taxonomic identifier

9986 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus

Protein attributes

Sequence length	671 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	This is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. May be considered as a novel component of the NF-kappa-B signaling axis responsible for the survival and activation of B-cells after BCR cross-linking By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Cofactor	Binds 3 calcium ions per subunit. The ions are bound to the C2 domain By similarity.
Subunit structure	Interacts with PDK1. Interacts with PHLPP1 and PHLPP2; both proteins mediate its dephosporylation By similarity.
Subcellular location	Cytoplasm By similarity. Membrane; Peripheral membrane protein By similarity.
Post-translational modification	Phosphorylation on 'Thr-499' of isoform beta-I, within the activation loop, renders it competent to autophosphorylate. Subsequent autophosphorylation of Thr-642 maintains catalytic competence, and autophosphorylation on Ser-661 appears to release the kinase into the cytosol. Similarly, isoform beta-II is autophosphorylated on 'Thr-640' and 'Ser-659', subsequent to phosphorylation on Thr-500. Autophosphorylated on other sites i.e. in the N-terminal and hinge regions have no effect on PKC activity By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 1 C2 domain. Contains 2 phorbol-ester/DAG-type zinc fingers. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Cytoplasm Membrane
Coding sequence diversity	Alternative splicing
Domain	Phorbol-ester binding Repeat Zinc-finger
Ligand	ATP-binding Calcium Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Acetylation Phosphoprotein
Gene Ontology (GO)
Biological process	intracellular signaling cascade Inferred from electronic annotation. Source: InterPro protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW diacylglycerol binding Inferred from electronic annotation. Source: UniProtKB-KW protein kinase C activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform Beta-I (identifier: P05772-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Beta-II (identifier: P05772-2)

The sequence of this isoform differs from the canonical sequence as follows:
     622-671: RDKRDTSNFD...YTNPEFVINV → CGRNAENFDR...SEFLKPEVKS

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 671	670	Protein kinase C beta type		PRO_0000055686
Regions
	Domain	173 – 260	88	C2
	Domain	342 – 600	259	Protein kinase
	Domain	601 – 671	71	AGC-kinase C-terminal
	Zinc finger	36 – 86	51	Phorbol-ester/DAG-type 1
	Zinc finger	101 – 151	51	Phorbol-ester/DAG-type 2
	Nucleotide binding	348 – 356	9	ATP By similarity
Sites
	Active site	466	1	Proton acceptor By similarity
	Metal binding	186	1	Calcium 1; via carbonyl oxygen By similarity
	Metal binding	187	1	Calcium 1 By similarity
	Metal binding	187	1	Calcium 2 By similarity
	Metal binding	193	1	Calcium 2 By similarity
	Metal binding	246	1	Calcium 1 By similarity
	Metal binding	246	1	Calcium 2 By similarity
	Metal binding	247	1	Calcium 2; via carbonyl oxygen By similarity
	Metal binding	248	1	Calcium 1 By similarity
	Metal binding	248	1	Calcium 2 By similarity
	Metal binding	248	1	Calcium 3 By similarity
	Metal binding	251	1	Calcium 3 By similarity
	Metal binding	252	1	Calcium 3; via carbonyl oxygen By similarity
	Metal binding	254	1	Calcium 1 By similarity
	Metal binding	254	1	Calcium 3 By similarity
	Binding site	371	1	ATP By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylalanine By similarity
	Modified residue	16	1	Phosphoserine; by autocatalysis Potential
	Modified residue	17	1	Phosphothreonine; by autocatalysis Potential
	Modified residue	195	1	Phosphotyrosine By similarity
	Modified residue	314	1	Phosphothreonine; by autocatalysis Potential
	Modified residue	324	1	Phosphothreonine; by autocatalysis Potential
	Modified residue	500	1	Phosphothreonine By similarity
	Modified residue	504	1	Phosphothreonine By similarity
	Modified residue	635	1	Phosphothreonine; by autocatalysis Potential
	Modified residue	642	1	Phosphothreonine Potential
	Modified residue	661	1	Phosphoserine; by autocatalysis By similarity
Natural variations
	Alternative sequence	622 – 671	50	RDKRD…FVINV → CGRNAENFDRFFTRHPPVLT PPDQEVIRNIDQSEFEGFSF VNSEFLKPEVKS in isoform Beta-II.		VSP_004740

Sequences

	Sequence		Length	Mass (Da)
	Isoform Beta-I [UniParc]. Last modified January 23, 2007. Version 3. Checksum: DFBF22EEB3D41861	FASTA	671	76,828
	10 20 30 40 50 60 MADPAAGQPP SEGEESTVRF ARKGALRQKN VHEVKNHKFT ARFFKQPTFC SHCTDFIWGF 70 80 90 100 110 120 GKQGFQCQVC CFVVHKRCHE FVTFSCPGAD KGPASDDPRS KHKFKIHTYS SPTFCDHCGS 130 140 150 160 170 180 LLYGLIHQGM KCDTCMMNVH KRCVMNVPSL CGTDHTERRG RIYIQAHIDR EVLIVVVRDA 190 200 210 220 230 240 KNLVPMDPNG LSDPYVKLKL IPDPKSESKQ KTKTIKCSLN PEWNETFRFQ LKESDKDRRL 250 260 270 280 290 300 SVEIWDWDLT SRNDFMGSLS FGISELQKAG VDGWFKLLSQ EEGEYFNVPV PPEGSEGNEE 310 320 330 340 350 360 LRQKFERAKI GQGTKTPEEK TTNTISKFDN NGNRDRMKLT DFNFLMVLGK GSFGKVMLSE 370 380 390 400 410 420 RKGTDELYAV KILKKDVVIQ DDDVECTMVE KRVLALPGKP PFLTQLHSCF QTMDRLYFVM 430 440 450 460 470 480 EYVNGGDLMY HIQQVGRFKE PHAVFYAAEI AIGLFFLQSK GIIYRDLKLD NVMLDSEGHI 490 500 510 520 530 540 KIADFGMCKE NIWDGVTTKT FCGTPDYIAP EIIAYQPYGK SVDWWAFGVL LYEMLAGQAP 550 560 570 580 590 600 FEGEDEDELF QSIMEHNVAY PKSMSKEAVA ICKGLMTKHP GKRLGCGPEG ERDIKDHAFF 610 620 630 640 650 660 RYIDWEKLER KEIQPPYKPK ARDKRDTSNF DKEFTRQPVE LTPTDKLFIM NLDQNEFAGF 670 SYTNPEFVIN V
	Isoform Beta-II. Checksum: C618FD86843C604B	FASTA	673	76,971
	10 20 30 40 50 60 MADPAAGQPP SEGEESTVRF ARKGALRQKN VHEVKNHKFT ARFFKQPTFC SHCTDFIWGF 70 80 90 100 110 120 GKQGFQCQVC CFVVHKRCHE FVTFSCPGAD KGPASDDPRS KHKFKIHTYS SPTFCDHCGS 130 140 150 160 170 180 LLYGLIHQGM KCDTCMMNVH KRCVMNVPSL CGTDHTERRG RIYIQAHIDR EVLIVVVRDA 190 200 210 220 230 240 KNLVPMDPNG LSDPYVKLKL IPDPKSESKQ KTKTIKCSLN PEWNETFRFQ LKESDKDRRL 250 260 270 280 290 300 SVEIWDWDLT SRNDFMGSLS FGISELQKAG VDGWFKLLSQ EEGEYFNVPV PPEGSEGNEE 310 320 330 340 350 360 LRQKFERAKI GQGTKTPEEK TTNTISKFDN NGNRDRMKLT DFNFLMVLGK GSFGKVMLSE 370 380 390 400 410 420 RKGTDELYAV KILKKDVVIQ DDDVECTMVE KRVLALPGKP PFLTQLHSCF QTMDRLYFVM 430 440 450 460 470 480 EYVNGGDLMY HIQQVGRFKE PHAVFYAAEI AIGLFFLQSK GIIYRDLKLD NVMLDSEGHI 490 500 510 520 530 540 KIADFGMCKE NIWDGVTTKT FCGTPDYIAP EIIAYQPYGK SVDWWAFGVL LYEMLAGQAP 550 560 570 580 590 600 FEGEDEDELF QSIMEHNVAY PKSMSKEAVA ICKGLMTKHP GKRLGCGPEG ERDIKDHAFF 610 620 630 640 650 660 RYIDWEKLER KEIQPPYKPK ACGRNAENFD RFFTRHPPVL TPPDQEVIRN IDQSEFEGFS 670 FVNSEFLKPE VKS

References

[1]

"Tissue-specific expression of three distinct types of rabbit protein kinase C." Ohno S., Kawasaki H., Imajoh S., Suzuki K., Inagaki M., Yokokura H., Sakoh T., Hidaka H. Nature 325:161-166(1987) [PubMed: 3808073] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS BETA-I AND BETA-II). Tissue: Brain.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X04795 mRNA. Translation: CAA28482.1. X04793 mRNA. Translation: CAA28480.1.
PIR	KIRBC2. A26037. KIRBC1. B26037.
RefSeq	NP_001095193.1. NP_001095195.1.
UniGene	Ocu.3267
3D structure databases
HSSP	HSSP built from PDB template 1A25 based on UniProtKB P04410.
SMR	P05772. Positions 94-158, 95-159, 156-287, 157-288, 339-668.
ModBase	Search...
Genome annotation databases
GeneID	100037719.
Organism-specific databases
CTD	100037719.
Phylogenomic databases
HOVERGEN	P05772.
Enzyme and pathway databases
BRENDA	2.7.11.13. 255.
Family and domain databases
InterPro	IPR000961. AGC-kinase_C. IPR000008. C2_Ca-dep. IPR018029. C2_membr_targeting. IPR020477. C2_region. IPR020454. DAG/PE_bd. IPR015745. PKC. IPR017892. Pkinase_C. IPR002219. Prot_Kinase_C-like_PE/DAG_bd. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR014375. Protein_kinase_C_a/b/g. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
PANTHER	PTHR22985:SF86. PKC. 1 hit.
Pfam	PF00130. C1_1. 2 hits. PF00168. C2. 1 hit. PF00069. Pkinase. 1 hit. PF00433. Pkinase_C. 1 hit. [Graphical view]
PIRSF	PIRSF000550. PKC_alpha. 1 hit.
PRINTS	PR00360. C2DOMAIN. PR00008. DAGPEDOMAIN.
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00109. C1. 2 hits. SM00239. C2. 1 hit. SM00133. S_TK_X. 1 hit. SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS51285. AGC_KINASE_CTER. 1 hit. PS50004. C2. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. PS00479. ZF_DAG_PE_1. 2 hits. PS50081. ZF_DAG_PE_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPCB_RABIT

Accession

Primary (citable) accession number: P05772
Secondary accession number(s): P05773

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1988
Last sequence update:	January 23, 2007
Last modified:	October 13, 2009
This is version 105 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)