P05766 (RS15_GEOSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
30S ribosomal protein S15

Alternative name(s):
BS18

Gene names

Name:

rpsO

Organism

Geobacillus stearothermophilus (Bacillus stearothermophilus)

Taxonomic identifier

1422 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Geobacillus

Protein attributes

Sequence length	89 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it helps nucleate assembly of the platform of the 30S subunit by binding and bridging several RNA helices of the 16S rRNA By similarity. HAMAP-Rule MF_01343 Forms an intersubunit bridge (bridge B4) with the 23S rRNA of the 50S subunit in the ribosome By similarity. HAMAP-Rule MF_01343
Subunit structure	Part of the 30S ribosomal subunit. Forms a bridge to the 50S subunit in the 70S ribosome, contacting the 23S rRNA By similarity.
Sequence similarities	Belongs to the ribosomal protein S15P family.

Ontologies

Keywords
Ligand	RNA-binding rRNA-binding
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological_process	translation Inferred from electronic annotation. Source: HAMAP
Cellular_component	ribosome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	rRNA binding Inferred from electronic annotation. Source: HAMAP structural constituent of ribosome Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.1 Ref.2

Chain

2 – 89

88

30S ribosomal protein S15 HAMAP-Rule MF_01343

PRO_0000115380

Experimental info

Sequence conflict

13 – 14

2

EQ → GE AA sequence Ref.2

Sequence conflict

46

1

H → R AA sequence Ref.1

Secondary structure

1

.

.

.

.

.

.

.

.

.

89

Helix Strand Turn

Details...

Helix

5 – 14

10

Helix

25 – 45

21

Helix

51 – 73

23

Helix

75 – 85

11

Sequences

	Sequence		Length	Mass (Da)
	P05766 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: B0387CC4B04DE000	FASTA	89	10,691
	10 20 30 40 50 60 MALTQERKRE IIEQFKVHEN DTGSPEVQIA ILTEQINNLN EHLRVHKKDH HSRRGLLKMV 70 80 GKRRRLLAYL RNKDVARYRE IVEKLGLRR

References

[1]	"Primary structures of ribosomal proteins from the archaebacterium Halobacterium marismortui and the eubacterium Bacillus stearothermophilus." Arndt E., Scholzen T., Kromer W., Hatakeyama T., Kimura M. Biochimie 73:657-668(1991) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-89.
[2]	"Procaryotic ribosomal proteins: N-terminal sequence homologies and structural correspondence of 30 S ribosomal proteins from Escherichia coli and Bacillus stearothermophilus." Yaguchi M., Matheson A.T., Visentin L.P. FEBS Lett. 46:296-300(1974) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-16. Strain: DSM 13240 / CIP 106956 / 10.
[3]	"Conformational variability of the N-terminal helix in the structure of ribosomal protein S15." Clemons W.M. Jr., Davies C., White S.W., Ramakrishnan V. Structure 6:429-438(1998) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS), SEQUENCE REVISION TO 46.

Cross-references

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1A32	X-ray	2.10	A	2-89	[»]

ProteinModelPortal

P05766.

SMR

P05766. Positions 3-87.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

Gene3D

1.10.287.10. 1 hit.

HAMAP

MF_01343_B. Ribosomal_S15_S13e_B.

InterPro

IPR000589. Ribosomal_S15.
IPR005290. Ribosomal_S15_bac-type.
IPR009068. S15_NS1_RNA-bd.
[Graphical view]

PANTHER

PTHR23321:SF10. PTHR23321:SF10. 1 hit.

Pfam

PF00312. Ribosomal_S15. 1 hit.
[Graphical view]

SUPFAM

SSF47060. S15/NS1_bind. 1 hit.

TIGRFAMs

TIGR00952. S15_bact. 1 hit.

PROSITE

PS00362. RIBOSOMAL_S15. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P05766.

Entry information

Entry name

RS15_GEOSE

Accession

Primary (citable) accession number: P05766

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1988
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 93 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program