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Protein

40S ribosomal protein S9-B

Gene

RPS9B

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel (PubMed:22096102). uS4 is involved in nucleolar processing of pre-18S ribosomal RNA and ribosome assembly (PubMed:15590835).1 Publication1 Publication

Miscellaneous

Present with 63300 molecules/cell in log phase SD medium.1 Publication
There are 2 genes for uS4 in yeast.Curated

GO - Molecular functioni

  • rRNA binding Source: GO_Central
  • structural constituent of ribosome Source: SGD

GO - Biological processi

  • maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  • positive regulation of translational fidelity Source: SGD
  • translation Source: InterPro

Keywordsi

Molecular functionRibonucleoprotein, Ribosomal protein, RNA-binding, rRNA-binding
Biological processRibosome biogenesis, rRNA processing

Enzyme and pathway databases

BioCyciYEAST:G3O-29132-MONOMER
ReactomeiR-SCE-156827 L13a-mediated translational silencing of Ceruloplasmin expression
R-SCE-1799339 SRP-dependent cotranslational protein targeting to membrane
R-SCE-6791226 Major pathway of rRNA processing in the nucleolus and cytosol
R-SCE-72689 Formation of a pool of free 40S subunits
R-SCE-72695 Formation of the ternary complex, and subsequently, the 43S complex
R-SCE-72702 Ribosomal scanning and start codon recognition
R-SCE-72706 GTP hydrolysis and joining of the 60S ribosomal subunit
R-SCE-975956 Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC)
R-SCE-975957 Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC)

Names & Taxonomyi

Protein namesi
Recommended name:
40S ribosomal protein S9-B1 Publication
Alternative name(s):
RP21
S13
Small ribosomal subunit protein uS4-B1 Publication
YP28
YS11
Gene namesi
Name:RPS9B1 Publication
Synonyms:RPS13B, SUP46
Ordered Locus Names:YBR189W
ORF Names:YBR1317
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBR189W
SGDiS000000393 RPS9B

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001327042 – 19540S ribosomal protein S9-BAdd BLAST194

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki180Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei184PhosphoserineCombined sources1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP05755
PaxDbiP05755
PRIDEiP05755
TopDownProteomicsiP05755

PTM databases

iPTMnetiP05755

Interactioni

Subunit structurei

Component of the small ribosomal subunit (SSU). Mature yeast ribosomes consist of a small (40S) and a large (60S) subunit. The 40S small subunit contains 1 molecule of ribosomal RNA (18S rRNA) and 33 different proteins (encoded by 57 genes). The large 60S subunit contains 3 rRNA molecules (25S, 5.8S and 5S rRNA) and 46 different proteins (encoded by 81 genes) (PubMed:9559554, PubMed:22096102). Interacts with snoRNA U3. uS11 interacts with MPP10. Component of the ribosomal small subunit (SSU) processome composed of at least 40 protein subunits and snoRNA U3 (PubMed:15590835).1 Publication2 Publications

Protein-protein interaction databases

BioGridi32887, 116 interactors
IntActiP05755, 30 interactors
MINTiP05755
STRINGi4932.YBR189W

Structurei

3D structure databases

ProteinModelPortaliP05755
SMRiP05755
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini107 – 181S4 RNA-bindingPROSITE-ProRule annotationAdd BLAST75

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00550000074829
HOGENOMiHOG000194525
InParanoidiP05755
KOiK02997
OMAiTHGHIAI
OrthoDBiEOG092C4UYC

Family and domain databases

CDDicd00165 S4, 1 hit
Gene3Di3.10.290.10, 1 hit
InterProiView protein in InterPro
IPR022801 Ribosomal_S4/S9
IPR005710 Ribosomal_S4/S9_euk/arc
IPR001912 Ribosomal_S4/S9_N
IPR018079 Ribosomal_S4_CS
IPR002942 S4_RNA-bd
IPR036986 S4_RNA-bd_sf
PANTHERiPTHR11831 PTHR11831, 1 hit
PfamiView protein in Pfam
PF01479 S4, 1 hit
SMARTiView protein in SMART
SM01390 Ribosomal_S4, 1 hit
SM00363 S4, 1 hit
TIGRFAMsiTIGR01018 uS4_arch, 1 hit
PROSITEiView protein in PROSITE
PS00632 RIBOSOMAL_S4, 1 hit
PS50889 S4, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P05755-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPRAPRTYSK TYSTPKRPYE SSRLDAELKL AGEFGLKNKR EIYRISFQLS
60 70 80 90 100
KIRRAARDLL TRDEKDPKRL FEGNALIRRL VRVGVLSEDK KKLDYVLALK
110 120 130 140 150
VEDFLERRLQ TQVYKLGLAK SVHHARVLIT QRHIAVGKQI VNIPSFMVRL
160 170 180 190
DSEKHIDFAP TSPFGGARPG RVARRNAARK AEASGEAAEE AEDEE
Length:195
Mass (Da):22,299
Last modified:January 23, 2007 - v4
Checksum:iF5BDCEA167003813
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti20 – 22ESS → QSB AA sequence (PubMed:6814480).Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M88650 Genomic DNA Translation: AAB59327.1
U02073 Genomic DNA Translation: AAB60283.1
Z36058 Genomic DNA Translation: CAA85151.1
BK006936 Genomic DNA Translation: DAA07304.1
PIRiS31287
RefSeqiNP_009748.3, NM_001178537.3

Genome annotation databases

EnsemblFungiiYBR189W; YBR189W; YBR189W
GeneIDi852487
KEGGisce:YBR189W

Similar proteinsi

Entry informationi

Entry nameiRS9B_YEAST
AccessioniPrimary (citable) accession number: P05755
Secondary accession number(s): D6VQI4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: January 23, 2007
Last modified: May 23, 2018
This is version 175 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health