40S ribosomal protein S9-B - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Contribute

Send feedback

Read comments (?) or add your own

P05755 (RS9B_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 121. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
40S ribosomal protein S9-B

Alternative name(s):
RP21
S13
YP28
YS11

Gene names

Name:	RPS9B
Synonyms:	RPS13B, SUP46
Ordered Locus Names:	YBR189W
ORF Names:	YBR1317

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	195 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in nucleolar processing of pre-18S ribosomal RNA and ribosome assembly. Ref.9
Subunit structure	Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). Interacts with snoRNA U3. Interacts with MPP10. Component of the ribosomal small subunit (SSU) processome composed of at least 40 protein subunits and snoRNA U3. Ref.6 Ref.9
Subcellular location	Cytoplasm. Nucleus › nucleolus Ref.7 Ref.9.
Miscellaneous	Present with 63300 molecules/cell in log phase SD medium. Ref.8 There are 2 genes for S9 in yeast.
Sequence similarities	Belongs to the ribosomal protein S4P family. Contains 1 S4 RNA-binding domain.

Ontologies

Keywords
Biological process	Ribosome biogenesis rRNA processing
Cellular component	Cytoplasm Nucleus
Ligand	RNA-binding rRNA-binding
Molecular function	Ribonucleoprotein Ribosomal protein
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Inferred from genetic interaction. Source: SGD positive regulation of translational fidelity Inferred from mutant phenotype. Source: SGD translation Inferred from electronic annotation. Source: InterPro
Cellular component	90S preribosome Inferred from direct assay. Source: SGD cytosolic small ribosomal subunit Inferred from direct assay Ref.5. Source: SGD nucleolus Inferred from electronic annotation. Source: UniProtKB-SubCell small-subunit processome Inferred from direct assay Ref.9. Source: SGD
Molecular function	rRNA binding Inferred from electronic annotation. Source: UniProtKB-KW structural constituent of ribosome Inferred from direct assay Ref.5. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed Ref.5

Chain

2 – 195

194

40S ribosomal protein S9-B

PRO_0000132704

Regions

Domain

107 – 181

S4 RNA-binding

Amino acid modifications

Modified residue

Phosphoserine Ref.11 Ref.14

Modified residue

Phosphoserine Ref.12

Modified residue

130

Phosphothreonine Ref.12

Modified residue

145

Phosphoserine Ref.14

Modified residue

161

Phosphothreonine Ref.14

Modified residue

162

Phosphoserine Ref.13 Ref.14

Modified residue

184

Phosphoserine Ref.10 Ref.11 Ref.14

Experimental info

Sequence conflict

20 – 22

ESS → QSB AA sequence Ref.5

Sequences

Sequence

Length

Mass (Da)

Tools

P05755 [UniParc].

Last modified January 23, 2007. Version 4.
Checksum: F5BDCEA167003813
Show »

FASTA

195

22,299

        10         20         30         40         50         60 
MPRAPRTYSK TYSTPKRPYE SSRLDAELKL AGEFGLKNKR EIYRISFQLS KIRRAARDLL 

        70         80         90        100        110        120 
TRDEKDPKRL FEGNALIRRL VRVGVLSEDK KKLDYVLALK VEDFLERRLQ TQVYKLGLAK 

       130        140        150        160        170        180 
SVHHARVLIT QRHIAVGKQI VNIPSFMVRL DSEKHIDFAP TSPFGGARPG RVARRNAARK 

       190 
AEASGEAAEE AEDEE

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The yeast omnipotent suppressor SUP46 encodes a ribosomal protein which is a functional and structural homolog of the Escherichia coli S4 ram protein." Vincent A., Liebman S.W. Genetics 132:375-386(1992) [PubMed: 1427034] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"A 12.5 kb fragment of the yeast chromosome II contains two adjacent genes encoding ribosomal proteins and six putative new genes, one of which encodes a putative transcriptional factor." Demolis N., Jacquet M., Mallet L. Yeast 10:1511-1525(1994) [PubMed: 7871891] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. Kleine K. EMBO J. 13:5795-5809(1994) [PubMed: 7813418] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Isolation of seventeen proteins and amino-terminal amino acid sequences of eight proteins from cytoplasmic ribosomes of yeast." Otaka E., Higo K., Osawa S. Biochemistry 21:4545-4550(1982) [PubMed: 6814480] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-24.
[6]	"The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae." Planta R.J., Mager W.H. Yeast 14:471-477(1998) [PubMed: 9559554] [Abstract] Cited for: NOMENCLATURE, SUBUNIT.
[7]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"The small-subunit processome is a ribosome assembly intermediate." Bernstein K.A., Gallagher J.E.G., Mitchell B.M., Granneman S., Baserga S.J. Eukaryot. Cell 3:1619-1626(2004) [PubMed: 15590835] [Abstract] Cited for: FUNCTION, INTERACTION WITH MPP10 AND SNORNA U3, IDENTIFICATION IN SSU PROCESSOME, SUBCELLULAR LOCATION.
[10]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed: 15665377] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-184, MASS SPECTROMETRY. Strain: YAL6B.
[11]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-9 AND SER-184, MASS SPECTROMETRY. Strain: ADR376.
[12]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-87 AND THR-130, MASS SPECTROMETRY.
[13]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-162, MASS SPECTROMETRY.
[14]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-9; SER-145; THR-161; SER-162 AND SER-184, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M88650 Genomic DNA. Translation: AAB59327.1. U02073 Genomic DNA. Translation: AAB60283.1. Z36058 Genomic DNA. Translation: CAA85151.1. BK006936 Genomic DNA. Translation: DAA07304.1.
PIR	S31287.
RefSeq	NP_009748.1. NM_001178537.1.
3D structure databases
ProteinModelPortal	P05755.
SMR	P05755. Positions 6-163.
ModBase	Search...
Protein-protein interaction databases
IntAct	P05755. 24 interactions.
MINT	MINT-2786155.
STRING	P05755.
Proteomic databases
PeptideAtlas	P05755.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YBR189W; YBR189W; YBR189W.
GeneID	852487.
KEGG	sce:YBR189W.
NMPDR	fig\|4932.3.peg.455.
Organism-specific databases
SGD	S000000393. RPS9B.
Phylogenomic databases
eggNOG	fuNOG07419.
GeneTree	EFGT00050000004096.
HOGENOM	HBG588353.
OMA	FIVHGHI.
OrthoDB	EOG4Q886R.
Gene expression databases
ArrayExpress	P05755.
Genevestigator	P05755.
GermOnline	YBR189W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR022801. Ribosomal_S4/S9. IPR005710. Ribosomal_S4/S9_euk/arc. IPR001912. Ribosomal_S4/S9_N. IPR018079. Ribosomal_S4_CS. IPR002942. S4_RNA-bd. [Graphical view]
Gene3D	G3DSA:3.10.290.10. G3DSA:3.10.290.10. 1 hit.
KO	K02997.
PANTHER	PTHR11831. Ribosomal_S4. 1 hit.
Pfam	PF00163. Ribosomal_S4. 1 hit. PF01479. S4. 1 hit. [Graphical view]
SMART	SM00363. S4. 1 hit. [Graphical view]
TIGRFAMs	TIGR01018. RpsD_arch. 1 hit.
PROSITE	PS00632. RIBOSOMAL_S4. 1 hit. PS50889. S4. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	971471.

Entry information

Entry name

RS9B_YEAST

Accession

Primary (citable) accession number: P05755
Secondary accession number(s): D6VQI4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1988
Last sequence update:	January 23, 2007
Last modified:	December 14, 2011
This is version 121 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome II

Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Ribosomal proteins

Ribosomal proteins families and list of entries

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents