Reviewed, UniProtKB/Swiss-Prot P05754 (RS8_YEAST)

Names and origin

Protein names

Recommended name:
    40S ribosomal protein S8
Alternative name(s):
    S14
    YS9
    RP19

Gene names

Name:	RPS8A
Synonyms:	RPS14A
Ordered Locus Names:	YBL072C
ORF Names:	YBL0613, YBL06.05
AND
Name:	RPS8B
Synonyms:	RPS14B
Ordered Locus Names:	YER102W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	200 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Subunit structure	Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). Ref.8
Subcellular location	Cytoplasm.
Miscellaneous	Present with 30300 molecules/cell in log phase SD medium. Ref.11 There are 2 genes for S8 in yeast.
Sequence similarities	Belongs to the ribosomal protein S8e family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Ribonucleoprotein Ribosomal protein
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Inferred from genetic interaction. Source: SGD translation Inferred by curator. Source: SGD
Cellular component	90S preribosome Inferred from direct assay. Source: SGD cytosolic small ribosomal subunit Inferred from direct assay. Source: SGD
Molecular function	structural constituent of ribosome Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.6 Ref.9
	Chain	2 – 200	199	40S ribosomal protein S8		PRO_0000122258
Amino acid modifications
	Modified residue	62	1	Phosphothreonine Ref.12
	Modified residue	66	1	Phosphoserine Ref.12
	Modified residue	69	1	Phosphoserine Ref.12
	Modified residue	73	1	Phosphoserine Ref.12
	Modified residue	107	1	Phosphothreonine Ref.13
	Modified residue	154	1	Phosphoserine Ref.13
	Modified residue	155	1	Phosphoserine Ref.13
	Modified residue	158	1	Phosphoserine Ref.13
Experimental info
	Sequence conflict	49	1	R → K AA sequence Ref.6
	Sequence conflict	101 – 102	2	IV → MS in ABM97467. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P05754-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 3DB5CD6B4970C457	FASTA	200	22,490
	10 20 30 40 50 60 MGISRDSRHK RSATGAKRAQ FRKKRKFELG RQPANTKIGA KRIHSVRTRG GNKKYRALRI 70 80 90 100 110 120 ETGNFSWASE GISKKTRIAG VVYHPSNNEL VRTNTLTKAA IVQIDATPFR QWFEAHYGQT 130 140 150 160 170 180 LGKKKNVKEE ETVAKSKNAE RKWAARAASA KIESSVESQF SAGRLYACIS SRPGQSGRCD 190 200 GYILEGEELA FYLRRLTAKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The two genes encoding yeast ribosomal protein S8 reside on different chromosomes, and are closely linked to the hsp70 stress protein genes SSA3 and SSA4." Logghe M., Molemans F., Fiers W., Contreras R. Yeast 10:1093-1100(1994) [PubMed: 7992509] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (RPS8A). Strain: ATCC 204508 / S288c.
[2]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed: 7813418] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (RPS8A). Strain: ATCC 204508 / S288c.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W. Nature 387:78-81(1997) [PubMed: 9169868] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (RPS8B). Strain: ATCC 204511 / S288c / AB972.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (RPS8B). Strain: ATCC 204508 / S288c.
[5]	"High-density yeast-tiling array reveals previously undiscovered introns and extensive regulation of meiotic splicing." Juneau K., Palm C., Miranda M., Davis R.W. Proc. Natl. Acad. Sci. U.S.A. 104:1522-1527(2007) [PubMed: 17244705] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-102 (RPS8A) AND 1-45 (RPS8B). Strain: ATCC 201390 / BY4743.
[6]	"Yeast ribosomal proteins. VIII. Isolation of two proteins and sequence characterization of twenty-four proteins from cytoplasmic ribosomes." Otaka E., Higo K., Itoh T. Mol. Gen. Genet. 195:544-546(1984) Cited for: PROTEIN SEQUENCE OF 2-51.
[7]	"Structure and regulation of the SSA4 HSP70 gene of Saccharomyces cerevisiae." Boorstein W.R., Craig E.A. J. Biol. Chem. 265:18912-18921(1990) [PubMed: 2121731] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 70-200 (RPS8B). Strain: DS10.
[8]	"The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae." Planta R.J., Mager W.H. Yeast 14:471-477(1998) [PubMed: 9559554] [Abstract] Cited for: NOMENCLATURE, SUBUNIT.
[9]	"The action of N-terminal acetyltransferases on yeast ribosomal proteins." Arnold R.J., Polevoda B., Reilly J.P., Sherman F. J. Biol. Chem. 274:37035-37040(1999) [PubMed: 10601260] [Abstract] Cited for: CLEAVAGE OF INITIATOR METHIONINE.
[10]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[11]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[12]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-62; SER-66; SER-69 AND SER-73, MASS SPECTROMETRY.
[13]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-107; SER-154; SER-155 AND SER-158, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z26879 Genomic DNA. Translation: CAA81525.1. Z35833 Genomic DNA. Translation: CAA84893.1. U18839 Genomic DNA. Translation: AAB64657.1. AY692824 Genomic DNA. Translation: AAT92843.1. J05637 Genomic DNA. Translation: AAA63573.1. EF123123 mRNA. Translation: ABM97467.1. EF123143 mRNA. Translation: ABM97487.1.
PIR	S45591.
RefSeq	NP_009481.1. NP_011028.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:1586N.
IntAct	P05754. 87 interactions.
STRING	P05754.
Proteomic databases
PRIDE	P05754.
Genome annotation databases
Ensembl	YBL072C; YBL072C; YBL072C; Saccharomyces cerevisiae. [Genome view] YER102W; YER102W; YER102W; Saccharomyces cerevisiae. [Genome view]
GeneID	852206. 856839.
KEGG	sce:YBL072C. sce:YER102W.
Organism-specific databases
CYGD	YBL072c. YER102w.
SGD	S000000168. RPS8A. S000000904. RPS8B.
Phylogenomic databases
HOGENOM	P05754.
OMA	VYHPSNN
OrthoDB	EOG9RV47N
Gene expression databases
ArrayExpress	P05754.
Genevestigator	P05754.
GermOnline	YBL072C. Saccharomyces cerevisiae. YER102W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001047. Ribosomal_S8e. IPR018283. Ribosomal_S8e_CS. [Graphical view]
Pfam	PF01201. Ribosomal_S8e. 1 hit. [Graphical view]
TIGRFAMs	TIGR00307. S8e. 1 hit.
PROSITE	PS01193. RIBOSOMAL_S8E. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	970706.

Entry information

Entry name

RS8_YEAST

Accession

Primary (citable) accession number: P05754
Secondary accession number(s): A2TBM0, A2TBP0, P22801

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1988
Last sequence update:	January 23, 2007
Last modified:	November 24, 2009
This is version 92 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)