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Protein

60S ribosomal protein L22-A

Gene

RPL22A

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

  1. structural constituent of ribosome Source: SGD

GO - Biological processi

  1. cytoplasmic translation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Enzyme and pathway databases

BioCyciYEAST:G3O-32215-MONOMER.
ReactomeiREACT_290856. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
REACT_302067. L13a-mediated translational silencing of Ceruloplasmin expression.
REACT_307259. Formation of a pool of free 40S subunits.
REACT_314339. SRP-dependent cotranslational protein targeting to membrane.
REACT_332565. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
REACT_343353. Peptide chain elongation.
REACT_346847. GTP hydrolysis and joining of the 60S ribosomal subunit.

Names & Taxonomyi

Protein namesi
Recommended name:
60S ribosomal protein L22-A
Alternative name(s):
L1c
RP4
YL31
Gene namesi
Name:RPL22A
Ordered Locus Names:YLR061W
ORF Names:L2168
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome XII

Organism-specific databases

SGDiS000004051. RPL22A.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytosolic large ribosomal subunit Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed2 Publications
Chaini2 – 12112060S ribosomal protein L22-APRO_0000215514Add
BLAST

Proteomic databases

MaxQBiP05749.
PaxDbiP05749.
PeptideAtlasiP05749.

Expressioni

Gene expression databases

GenevestigatoriP05749.

Interactioni

Subunit structurei

Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S).1 Publication

Protein-protein interaction databases

BioGridi31336. 92 interactions.
IntActiP05749. 4 interactions.
MINTiMINT-4494605.
STRINGi4932.YLR061W.

Structurei

Secondary structure

1
121
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi13 – 186Combined sources
Helixi20 – 234Combined sources
Turni24 – 263Combined sources
Helixi30 – 389Combined sources
Beta strandi42 – 454Combined sources
Beta strandi46 – 483Combined sources
Turni49 – 535Combined sources
Beta strandi54 – 585Combined sources
Beta strandi60 – 7011Combined sources
Helixi73 – 8614Combined sources
Turni90 – 923Combined sources
Beta strandi94 – 996Combined sources
Beta strandi102 – 1065Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3J6Xelectron microscopy6.10621-121[»]
3J6Yelectron microscopy6.10621-121[»]
3J77electron microscopy6.20721-121[»]
3J78electron microscopy6.30721-121[»]
4U3MX-ray3.00N2/n22-121[»]
4U3NX-ray3.20N2/n22-121[»]
4U3UX-ray2.90N2/n22-121[»]
4U4NX-ray3.10N2/n22-121[»]
4U4OX-ray3.60N2/n22-121[»]
4U4QX-ray3.00N2/n22-121[»]
4U4RX-ray2.80N2/n22-121[»]
4U4UX-ray3.00N2/n22-121[»]
4U4YX-ray3.20N2/n22-121[»]
4U4ZX-ray3.10N2/n22-121[»]
4U50X-ray3.20N2/n22-121[»]
4U51X-ray3.20N2/n22-121[»]
4U52X-ray3.00N2/n22-121[»]
4U53X-ray3.30N2/n22-121[»]
4U55X-ray3.20N2/n22-121[»]
4U56X-ray3.45N2/n22-121[»]
4U6FX-ray3.10N2/n22-121[»]
4V6Ielectron microscopy8.80BW1-121[»]
4V7Felectron microscopy8.70V1-121[»]
4V88X-ray3.00BU/DU1-121[»]
4V8Telectron microscopy8.10U1-121[»]
4V8Yelectron microscopy4.30BU2-121[»]
4V8Zelectron microscopy6.60BU2-121[»]
4V91electron microscopy3.70U1-121[»]
ProteinModelPortaliP05749.
SMRiP05749. Positions 9-108.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ribosomal protein L22e family.Curated

Phylogenomic databases

eggNOGiNOG260326.
GeneTreeiENSGT00390000003719.
HOGENOMiHOG000198396.
InParanoidiP05749.
KOiK02891.
OrthoDBiEOG7673PN.

Family and domain databases

InterProiIPR002671. Ribosomal_L22e.
[Graphical view]
PANTHERiPTHR10064. PTHR10064. 1 hit.
PfamiPF01776. Ribosomal_L22e. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P05749-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPNTSRKQK IAKTFTVDVS SPTENGVFDP ASYAKYLIDH IKVEGAVGNL
60 70 80 90 100
GNAVTVTEDG TVVTVVSTAK FSGKYLKYLT KKYLKKNQLR DWIRFVSTKT
110 120
NEYRLAFYQV TPEEDEEEDE E
Length:121
Mass (Da):13,693
Last modified:January 23, 2007 - v3
Checksum:iAFF542E484A52069
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti49 – 491N → D AA sequence (PubMed:6814480).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X94607 Genomic DNA. Translation: CAA64308.1.
Z73233 Genomic DNA. Translation: CAA97592.1.
BK006945 Genomic DNA. Translation: DAA09379.1.
PIRiS61635.
RefSeqiNP_013162.1. NM_001181948.1.

Genome annotation databases

EnsemblFungiiYLR061W; YLR061W; YLR061W.
GeneIDi850750.
KEGGisce:YLR061W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X94607 Genomic DNA. Translation: CAA64308.1.
Z73233 Genomic DNA. Translation: CAA97592.1.
BK006945 Genomic DNA. Translation: DAA09379.1.
PIRiS61635.
RefSeqiNP_013162.1. NM_001181948.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3J6Xelectron microscopy6.10621-121[»]
3J6Yelectron microscopy6.10621-121[»]
3J77electron microscopy6.20721-121[»]
3J78electron microscopy6.30721-121[»]
4U3MX-ray3.00N2/n22-121[»]
4U3NX-ray3.20N2/n22-121[»]
4U3UX-ray2.90N2/n22-121[»]
4U4NX-ray3.10N2/n22-121[»]
4U4OX-ray3.60N2/n22-121[»]
4U4QX-ray3.00N2/n22-121[»]
4U4RX-ray2.80N2/n22-121[»]
4U4UX-ray3.00N2/n22-121[»]
4U4YX-ray3.20N2/n22-121[»]
4U4ZX-ray3.10N2/n22-121[»]
4U50X-ray3.20N2/n22-121[»]
4U51X-ray3.20N2/n22-121[»]
4U52X-ray3.00N2/n22-121[»]
4U53X-ray3.30N2/n22-121[»]
4U55X-ray3.20N2/n22-121[»]
4U56X-ray3.45N2/n22-121[»]
4U6FX-ray3.10N2/n22-121[»]
4V6Ielectron microscopy8.80BW1-121[»]
4V7Felectron microscopy8.70V1-121[»]
4V88X-ray3.00BU/DU1-121[»]
4V8Telectron microscopy8.10U1-121[»]
4V8Yelectron microscopy4.30BU2-121[»]
4V8Zelectron microscopy6.60BU2-121[»]
4V91electron microscopy3.70U1-121[»]
ProteinModelPortaliP05749.
SMRiP05749. Positions 9-108.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31336. 92 interactions.
IntActiP05749. 4 interactions.
MINTiMINT-4494605.
STRINGi4932.YLR061W.

Proteomic databases

MaxQBiP05749.
PaxDbiP05749.
PeptideAtlasiP05749.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLR061W; YLR061W; YLR061W.
GeneIDi850750.
KEGGisce:YLR061W.

Organism-specific databases

SGDiS000004051. RPL22A.

Phylogenomic databases

eggNOGiNOG260326.
GeneTreeiENSGT00390000003719.
HOGENOMiHOG000198396.
InParanoidiP05749.
KOiK02891.
OrthoDBiEOG7673PN.

Enzyme and pathway databases

BioCyciYEAST:G3O-32215-MONOMER.
ReactomeiREACT_290856. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
REACT_302067. L13a-mediated translational silencing of Ceruloplasmin expression.
REACT_307259. Formation of a pool of free 40S subunits.
REACT_314339. SRP-dependent cotranslational protein targeting to membrane.
REACT_332565. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
REACT_343353. Peptide chain elongation.
REACT_346847. GTP hydrolysis and joining of the 60S ribosomal subunit.

Miscellaneous databases

NextBioi966882.
PROiP05749.

Gene expression databases

GenevestigatoriP05749.

Family and domain databases

InterProiIPR002671. Ribosomal_L22e.
[Graphical view]
PANTHERiPTHR10064. PTHR10064. 1 hit.
PfamiPF01776. Ribosomal_L22e. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
    Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
    , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
    Nature 387:87-90(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Isolation of seventeen proteins and amino-terminal amino acid sequences of eight proteins from cytoplasmic ribosomes of yeast."
    Otaka E., Higo K., Osawa S.
    Biochemistry 21:4545-4550(1981) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-52.
  4. "The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae."
    Planta R.J., Mager W.H.
    Yeast 14:471-477(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NOMENCLATURE, SUBUNIT.
  5. "The action of N-terminal acetyltransferases on yeast ribosomal proteins."
    Arnold R.J., Polevoda B., Reilly J.P., Sherman F.
    J. Biol. Chem. 274:37035-37040(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: CLEAVAGE OF INITIATOR METHIONINE.
  6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Crystal structure of the eukaryotic ribosome."
    Ben-Shem A., Jenner L., Yusupova G., Yusupov M.
    Science 330:1203-1209(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (4.0 ANGSTROMS) OF 80S RIBOSOME.
  10. Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF 80S RIBOSOME.

Entry informationi

Entry nameiRL22A_YEAST
AccessioniPrimary (citable) accession number: P05749
Secondary accession number(s): D6VY63
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: January 23, 2007
Last modified: April 1, 2015
This is version 118 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 60400 molecules/cell in log phase SD medium.1 Publication
There are 2 genes for L22 in yeast.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.