P05748 (RL15A_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
60S ribosomal protein L15-A

Alternative name(s):
L13
RP15R
YL10
YP18

Gene names

Name:	RPL15A
Synonyms:	RPL10A, RPL13A, YL10A
Ordered Locus Names:	YLR029C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	204 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). Ref.9
Subcellular location	Cytoplasm By similarity.
Miscellaneous	There are 2 genes for L15 in yeast.
Sequence similarities	Belongs to the ribosomal protein L15e family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Ribonucleoprotein Ribosomal protein
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	cytoplasmic translation Inferred from direct assay Ref.4. Source: SGD
Cellular component	cytosolic large ribosomal subunit Inferred from direct assay Ref.4. Source: SGD
Molecular function	RNA binding Inferred from direct assay. Source: SGD structural constituent of ribosome Inferred from direct assay Ref.4. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.4 Ref.5
	Chain	2 – 204	203	60S ribosomal protein L15-A		PRO_0000127566
Amino acid modifications
	Modified residue	198	1	Phosphoserine Ref.6
Experimental info
	Sequence conflict	28	1	W → G AA sequence Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P05748 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 3913F9E4AB9ECA2C	FASTA	204	24,422
	10 20 30 40 50 60 MGAYKYLEEL QRKKQSDVLR FLQRVRVWEY RQKNVIHRAA RPTRPDKARR LGYKAKQGFV 70 80 90 100 110 120 IYRVRVRRGN RKRPVPKGAT YGKPTNQGVN ELKYQRSLRA TAEERVGRRA ANLRVLNSYW 130 140 150 160 170 180 VNQDSTYKYF EVILVDPQHK AIRRDARYNW ICDPVHKHRE ARGLTATGKK SRGINKGHKF 190 200 NNTKAGRRKT WKRQNTLSLW RYRK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Tomiyoshi A., Suzuki K., Otaka E. Submitted (MAR-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D. Nature 387:87-90(1997) [PubMed: 9169871] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Isolation of seventeen proteins and amino-terminal amino acid sequences of eight proteins from cytoplasmic ribosomes of yeast." Otaka E., Higo K., Osawa S. Biochemistry 21:4545-4550(1982) [PubMed: 6814480] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-44.
[5]	"NH2-terminal acetylation of ribosomal proteins of Saccharomyces cerevisiae." Takakura H., Tsunasawa S., Miyagi M., Warner J.R. J. Biol. Chem. 267:5442-5445(1992) [PubMed: 1544921] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-9.
[6]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-198, MASS SPECTROMETRY.
[7]	"Structure of the 80S ribosome from Saccharomyces cerevisiae -- tRNA-ribosome and subunit-subunit interactions." Spahn C.M.T., Beckmann R., Eswar N., Penczek P.A., Sali A., Blobel G., Frank J. Cell 107:373-386(2001) [PubMed: 11701127] [Abstract] Cited for: 3D-STRUCTURE MODELING OF 3-196, ELECTRON MICROSCOPY.
[8]	"Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation." Spahn C.M.T., Gomez-Lorenzo M.G., Grassucci R.A., Joergensen R., Andersen G.R., Beckmann R., Penczek P.A., Ballesta J.P.G., Frank J. EMBO J. 23:1008-1019(2004) [PubMed: 14976550] [Abstract] Cited for: 3D-STRUCTURE MODELING, ELECTRON MICROSCOPY.
[9]	"The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae." Planta R.J., Mager W.H. Yeast 14:471-477(1998) [PubMed: 9559554] [Abstract] Cited for: NOMENCLATURE, SUBUNIT.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

D14675 Genomic DNA. Translation: BAA03506.1.
Z73201 Genomic DNA. Translation: CAA97553.1.
BK006945 Genomic DNA. Translation: DAA09347.1.

PIR

S48502.

RefSeq

NP_013129.1. NM_001181916.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1K5Y	model	-	L	3-196	[»]
1S1I	electron microscopy	11.70	L	2-204	[»]
3IZS	electron microscopy	-	P	1-204	[»]
3O58	X-ray	4.00	O	1-204	[»]
3O5H	X-ray	4.00	O	1-204	[»]

ProteinModelPortal

P05748.

SMR

P05748. Positions 3-189.

ModBase

Search...

Protein-protein interaction databases

IntAct

P05748. 17 interactions.

MINT

MINT-1324900.

STRING

P05748.

Proteomic databases

PeptideAtlas

P05748.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YLR029C; YLR029C; YLR029C.

GeneID

850716.

KEGG

sce:YLR029C.

Organism-specific databases

SGD

S000004019. RPL15A.

Phylogenomic databases

eggNOG

fuNOG06002.

GeneTree

EFGT00050000006622.

HOGENOM

HBG577798.

OMA

AKQGIIV.

OrthoDB

EOG4M3DJQ.

Gene expression databases

ArrayExpress

P05748.

Genevestigator

P05748.

GermOnline

YLR029C. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR024794. Rbsml_L15e_core_dom.
IPR000439. Ribosomal_L15e.
IPR020925. Ribosomal_L15e_CS.
IPR012678. Ribosomal_L23/L15e_core_dom.
[Graphical view]

Gene3D

G3DSA:3.40.1120.10. Ribosomal_L15e. 1 hit.

KO

K02877.

PANTHER

PTHR11847. Ribosomal_L15e. 1 hit.

Pfam

PF00827. Ribosomal_L15e. 1 hit.
[Graphical view]

SUPFAM

SSF54189. L23_L15e_core. 1 hit.

PROSITE

PS01194. RIBOSOMAL_L15E. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

966784.

Entry information

Entry name

RL15A_YEAST

Accession

Primary (citable) accession number: P05748
Secondary accession number(s): D6VY31

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1988
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 106 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program