Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

60S ribosomal protein L29

Gene

RPL29

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

  • structural constituent of ribosome Source: SGD

GO - Biological processi

  • cytoplasmic translation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Enzyme and pathway databases

BioCyciYEAST:G3O-30506-MONOMER.
ReactomeiREACT_290856. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
REACT_302067. L13a-mediated translational silencing of Ceruloplasmin expression.
REACT_307259. Formation of a pool of free 40S subunits.
REACT_314339. SRP-dependent cotranslational protein targeting to membrane.
REACT_332565. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
REACT_343353. Peptide chain elongation.
REACT_346847. GTP hydrolysis and joining of the 60S ribosomal subunit.

Names & Taxonomyi

Protein namesi
Recommended name:
60S ribosomal protein L29
Alternative name(s):
YL43
Gene namesi
Name:RPL29
Synonyms:YL43
Ordered Locus Names:YFR032C-A
ORF Names:YFR032BW
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome VI

Organism-specific databases

EuPathDBiFungiDB:YFR032C-A.
SGDiS000006437. RPL29.

Subcellular locationi

GO - Cellular componenti

  • cytosolic large ribosomal subunit Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed2 Publications
Chaini2 – 595860S ribosomal protein L29PRO_0000219141Add
BLAST

Proteomic databases

MaxQBiP05747.
PaxDbiP05747.
PeptideAtlasiP05747.

Interactioni

Subunit structurei

Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S).1 Publication

Protein-protein interaction databases

BioGridi31189. 74 interactions.
IntActiP05747. 1 interaction.
MINTiMINT-2493087.

Structurei

Secondary structure

1
59
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi12 – 187Combined sources
Helixi37 – 5519Combined sources
Helixi56 – 583Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3J6Xelectron microscopy6.10691-59[»]
3J6Yelectron microscopy6.10691-59[»]
3J77electron microscopy6.20791-59[»]
3J78electron microscopy6.30791-59[»]
4U3MX-ray3.00N9/n92-59[»]
4U3NX-ray3.20N9/n92-59[»]
4U3UX-ray2.90N9/n92-59[»]
4U4NX-ray3.10N9/n92-59[»]
4U4OX-ray3.60N9/n92-59[»]
4U4QX-ray3.00N9/n92-59[»]
4U4RX-ray2.80N9/n92-59[»]
4U4UX-ray3.00N9/n92-59[»]
4U4YX-ray3.20N9/n92-59[»]
4U4ZX-ray3.10N9/n92-59[»]
4U50X-ray3.20N9/n92-59[»]
4U51X-ray3.20N9/n92-59[»]
4U52X-ray3.00N9/n92-59[»]
4U53X-ray3.30N9/n92-59[»]
4U55X-ray3.20N9/n92-59[»]
4U56X-ray3.45N9/n92-59[»]
4U6FX-ray3.10N9/n92-59[»]
4V6Ielectron microscopy8.80Bd1-59[»]
4V88X-ray3.00Bb/Db1-59[»]
4V8Telectron microscopy8.10b1-59[»]
4V8Yelectron microscopy4.30Bb2-59[»]
4V8Zelectron microscopy6.60Bb2-59[»]
4V91electron microscopy3.70b1-59[»]
ProteinModelPortaliP05747.
SMRiP05747. Positions 2-50.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ribosomal protein L29e family.Curated

Phylogenomic databases

eggNOGiNOG249125.
GeneTreeiENSGT00390000007084.
HOGENOMiHOG000212220.
InParanoidiP05747.
KOiK02905.
OMAiSKFRRNH.
OrthoDBiEOG7BGJ0F.

Family and domain databases

InterProiIPR002673. Ribosomal_L29e.
[Graphical view]
PANTHERiPTHR12884. PTHR12884. 1 hit.
PfamiPF01779. Ribosomal_L29e. 1 hit.
[Graphical view]
ProDomiPD010314. Ribosomal_L29e. 1 hit.
[Graphical view] [Entries sharing at least one domain]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P05747-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKSKNHTAH NQTRKAHRNG IKKPKTYKYP SLKGVDPKFR RNHKHALHGT

AKALAAAKK
Length:59
Mass (Da):6,669
Last modified:January 23, 2007 - v3
Checksum:iACED5822295F53F7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50617 Genomic DNA. No translation available.
AY558472 Genomic DNA. Translation: AAS56798.1.
EF123141 mRNA. Translation: ABM97485.1.
BK006940 Genomic DNA. Translation: DAA12474.1.
PIRiS71066.
RefSeqiNP_116690.3. NM_001184311.3.

Genome annotation databases

EnsemblFungiiYFR032C-A; YFR032C-A; YFR032C-A.
GeneIDi850592.
KEGGisce:YFR032C-A.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50617 Genomic DNA. No translation available.
AY558472 Genomic DNA. Translation: AAS56798.1.
EF123141 mRNA. Translation: ABM97485.1.
BK006940 Genomic DNA. Translation: DAA12474.1.
PIRiS71066.
RefSeqiNP_116690.3. NM_001184311.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3J6Xelectron microscopy6.10691-59[»]
3J6Yelectron microscopy6.10691-59[»]
3J77electron microscopy6.20791-59[»]
3J78electron microscopy6.30791-59[»]
4U3MX-ray3.00N9/n92-59[»]
4U3NX-ray3.20N9/n92-59[»]
4U3UX-ray2.90N9/n92-59[»]
4U4NX-ray3.10N9/n92-59[»]
4U4OX-ray3.60N9/n92-59[»]
4U4QX-ray3.00N9/n92-59[»]
4U4RX-ray2.80N9/n92-59[»]
4U4UX-ray3.00N9/n92-59[»]
4U4YX-ray3.20N9/n92-59[»]
4U4ZX-ray3.10N9/n92-59[»]
4U50X-ray3.20N9/n92-59[»]
4U51X-ray3.20N9/n92-59[»]
4U52X-ray3.00N9/n92-59[»]
4U53X-ray3.30N9/n92-59[»]
4U55X-ray3.20N9/n92-59[»]
4U56X-ray3.45N9/n92-59[»]
4U6FX-ray3.10N9/n92-59[»]
4V6Ielectron microscopy8.80Bd1-59[»]
4V88X-ray3.00Bb/Db1-59[»]
4V8Telectron microscopy8.10b1-59[»]
4V8Yelectron microscopy4.30Bb2-59[»]
4V8Zelectron microscopy6.60Bb2-59[»]
4V91electron microscopy3.70b1-59[»]
ProteinModelPortaliP05747.
SMRiP05747. Positions 2-50.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31189. 74 interactions.
IntActiP05747. 1 interaction.
MINTiMINT-2493087.

Proteomic databases

MaxQBiP05747.
PaxDbiP05747.
PeptideAtlasiP05747.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYFR032C-A; YFR032C-A; YFR032C-A.
GeneIDi850592.
KEGGisce:YFR032C-A.

Organism-specific databases

EuPathDBiFungiDB:YFR032C-A.
SGDiS000006437. RPL29.

Phylogenomic databases

eggNOGiNOG249125.
GeneTreeiENSGT00390000007084.
HOGENOMiHOG000212220.
InParanoidiP05747.
KOiK02905.
OMAiSKFRRNH.
OrthoDBiEOG7BGJ0F.

Enzyme and pathway databases

BioCyciYEAST:G3O-30506-MONOMER.
ReactomeiREACT_290856. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
REACT_302067. L13a-mediated translational silencing of Ceruloplasmin expression.
REACT_307259. Formation of a pool of free 40S subunits.
REACT_314339. SRP-dependent cotranslational protein targeting to membrane.
REACT_332565. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
REACT_343353. Peptide chain elongation.
REACT_346847. GTP hydrolysis and joining of the 60S ribosomal subunit.

Miscellaneous databases

NextBioi966443.
PROiP05747.

Family and domain databases

InterProiIPR002673. Ribosomal_L29e.
[Graphical view]
PANTHERiPTHR12884. PTHR12884. 1 hit.
PfamiPF01779. Ribosomal_L29e. 1 hit.
[Graphical view]
ProDomiPD010314. Ribosomal_L29e. 1 hit.
[Graphical view] [Entries sharing at least one domain]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. "High-density yeast-tiling array reveals previously undiscovered introns and extensive regulation of meiotic splicing."
    Juneau K., Palm C., Miranda M., Davis R.W.
    Proc. Natl. Acad. Sci. U.S.A. 104:1522-1527(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-47.
    Strain: ATCC 201390 / BY4743.
  5. "Yeast ribosomal proteins. VIII. Isolation of two proteins and sequence characterization of twenty-four proteins from cytoplasmic ribosomes."
    Otaka E., Higo K., Itoh T.
    Mol. Gen. Genet. 195:544-546(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-40, CLEAVAGE OF INITIATOR METHIONINE.
  6. "The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae."
    Planta R.J., Mager W.H.
    Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NOMENCLATURE, SUBUNIT.
  7. "The action of N-terminal acetyltransferases on yeast ribosomal proteins."
    Arnold R.J., Polevoda B., Reilly J.P., Sherman F.
    J. Biol. Chem. 274:37035-37040(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: CLEAVAGE OF INITIATOR METHIONINE.
  8. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
    Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
    Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. "Crystal structure of the eukaryotic ribosome."
    Ben-Shem A., Jenner L., Yusupova G., Yusupov M.
    Science 330:1203-1209(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (4.0 ANGSTROMS) OF 80S RIBOSOME.
  13. Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF 80S RIBOSOME.

Entry informationi

Entry nameiRL29_YEAST
AccessioniPrimary (citable) accession number: P05747
Secondary accession number(s): A2TBN8, D6VTR4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: January 23, 2007
Last modified: July 22, 2015
This is version 128 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 51100 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome VI
    Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.