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Protein

60S ribosomal protein L33-A

Gene

RPL33A

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

  1. structural constituent of ribosome Source: SGD

GO - Biological processi

  1. cytoplasmic translation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Enzyme and pathway databases

BioCyciYEAST:G3O-34040-MONOMER.
ReactomeiREACT_188965. SRP-dependent cotranslational protein targeting to membrane.
REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
REACT_217188. Formation of a pool of free 40S subunits.
REACT_232946. GTP hydrolysis and joining of the 60S ribosomal subunit.
REACT_252688. L13a-mediated translational silencing of Ceruloplasmin expression.
REACT_257951. Peptide chain elongation.

Names & Taxonomyi

Protein namesi
Recommended name:
60S ribosomal protein L33-A
Alternative name(s):
L37
RP47
YL37
Gene namesi
Name:RPL33A
Synonyms:RPL37A
Ordered Locus Names:YPL143W
ORF Names:LPI4W, P2625
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XVI

Organism-specific databases

SGDiS000006064. RPL33A.

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytosolic large ribosomal subunit Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed2 Publications
Chaini2 – 10710660S ribosomal protein L33-APRO_0000192807Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine; partial1 Publication

Post-translational modificationi

N-terminally acetylated by acetyltransferase NatA.1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP05744.
PeptideAtlasiP05744.

Expressioni

Gene expression databases

GenevestigatoriP05744.

Interactioni

Subunit structurei

Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S).1 Publication

Protein-protein interaction databases

BioGridi36040. 48 interactions.
IntActiP05744. 14 interactions.
MINTiMINT-8285485.
STRINGi4932.YPL143W.

Structurei

Secondary structure

1
107
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi8 – 1811Combined sources
Beta strandi23 – 3210Combined sources
Helixi38 – 414Combined sources
Turni42 – 465Combined sources
Beta strandi48 – 547Combined sources
Beta strandi59 – 613Combined sources
Beta strandi63 – 7412Combined sources
Beta strandi76 – 794Combined sources
Beta strandi81 – 877Combined sources
Turni92 – 954Combined sources
Beta strandi97 – 1015Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1VW8electron microscopy6.10g1-107[»]
1VWUelectron microscopy6.10g1-107[»]
1VXVelectron microscopy6.20g1-107[»]
1VXYelectron microscopy6.30g1-107[»]
3IZSelectron microscopy-j1-107[»]
3J65electron microscopy8.70e1-107[»]
3U5EX-ray3.00f1-107[»]
3U5IX-ray3.00f1-107[»]
4B6Aelectron microscopy8.10f1-107[»]
4BYNelectron microscopy4.30f2-107[»]
4BYUelectron microscopy6.60f2-107[»]
4CUWelectron microscopy3.70f1-107[»]
4UJHX-ray3.20S2-107[»]
4UJJX-ray3.20X2-107[»]
4UJMX-ray3.00S2-107[»]
4UJOX-ray3.00X2-107[»]
4UJRX-ray2.80S2-107[»]
4UJTX-ray2.80X2-107[»]
4UJWX-ray3.10S2-107[»]
4UJYX-ray3.10W2-107[»]
4UK1X-ray3.00S2-107[»]
4UK3X-ray3.00X2-107[»]
4UK6X-ray3.20S2-107[»]
4UK8X-ray3.20X2-107[»]
4UKBX-ray2.90S2-107[»]
4UKEX-ray2.90X2-107[»]
4UKHX-ray3.45S2-107[»]
4UKJX-ray3.45W2-107[»]
4UKMX-ray3.20S2-107[»]
4UKOX-ray3.20W2-107[»]
4UKRX-ray3.30S2-107[»]
4UKTX-ray3.30X2-107[»]
4UKWX-ray3.10S2-107[»]
4UKYX-ray3.10X2-107[»]
4UL1X-ray3.60S2-107[»]
4UL3X-ray3.60X2-107[»]
4UL6X-ray3.00S2-107[»]
4UL8X-ray3.00X2-107[»]
4ULBX-ray3.00S2-107[»]
4ULDX-ray3.00X2-107[»]
4ULGX-ray3.20S2-107[»]
4ULIX-ray3.20X2-107[»]
4ULMX-ray3.10S2-107[»]
4ULOX-ray3.10X2-107[»]
4ULRX-ray3.20S2-107[»]
4ULTX-ray3.20W2-107[»]
4V7Felectron microscopy8.70e1-107[»]
4V8Telectron microscopy8.10f1-107[»]
ProteinModelPortaliP05744.
SMRiP05744. Positions 2-107.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ribosomal protein L35Ae family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000016972.
HOGENOMiHOG000195636.
InParanoidiP05744.
KOiK02917.
OMAiISYQRSK.
OrthoDBiEOG7DFXRH.

Family and domain databases

InterProiIPR001780. Ribosomal_L35A.
IPR018266. Ribosomal_L35Ae_CS.
IPR009000. Transl_B-barrel.
[Graphical view]
PANTHERiPTHR10902. PTHR10902. 1 hit.
PfamiPF01247. Ribosomal_L35Ae. 1 hit.
[Graphical view]
ProDomiPD012670. Ribosomal_L35Ae. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF50447. SSF50447. 1 hit.
PROSITEiPS01105. RIBOSOMAL_L35AE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P05744-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAESHRLYVK GKHLSYQRSK RVNNPNVSLI KIEGVATPQD AQFYLGKRIA
60 70 80 90 100
YVYRASKEVR GSKIRVMWGK VTRTHGNSGV VRATFRNNLP AKTFGASVRI

FLYPSNI
Length:107
Mass (Da):12,154
Last modified:January 23, 2007 - v3
Checksum:i263E8389218B5027
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti24 – 241N → D AA sequence (PubMed:18782943)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X57969 Genomic DNA. Translation: CAA41035.1.
U43703 Genomic DNA. Translation: AAB68218.1.
Z73499 Genomic DNA. Translation: CAA97847.1.
BK006949 Genomic DNA. Translation: DAA11292.1.
PIRiS18431.
RefSeqiNP_015182.1. NM_001183957.1.

Genome annotation databases

EnsemblFungiiYPL143W; YPL143W; YPL143W.
GeneIDi855960.
KEGGisce:YPL143W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X57969 Genomic DNA. Translation: CAA41035.1.
U43703 Genomic DNA. Translation: AAB68218.1.
Z73499 Genomic DNA. Translation: CAA97847.1.
BK006949 Genomic DNA. Translation: DAA11292.1.
PIRiS18431.
RefSeqiNP_015182.1. NM_001183957.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1VW8electron microscopy6.10g1-107[»]
1VWUelectron microscopy6.10g1-107[»]
1VXVelectron microscopy6.20g1-107[»]
1VXYelectron microscopy6.30g1-107[»]
3IZSelectron microscopy-j1-107[»]
3J65electron microscopy8.70e1-107[»]
3U5EX-ray3.00f1-107[»]
3U5IX-ray3.00f1-107[»]
4B6Aelectron microscopy8.10f1-107[»]
4BYNelectron microscopy4.30f2-107[»]
4BYUelectron microscopy6.60f2-107[»]
4CUWelectron microscopy3.70f1-107[»]
4UJHX-ray3.20S2-107[»]
4UJJX-ray3.20X2-107[»]
4UJMX-ray3.00S2-107[»]
4UJOX-ray3.00X2-107[»]
4UJRX-ray2.80S2-107[»]
4UJTX-ray2.80X2-107[»]
4UJWX-ray3.10S2-107[»]
4UJYX-ray3.10W2-107[»]
4UK1X-ray3.00S2-107[»]
4UK3X-ray3.00X2-107[»]
4UK6X-ray3.20S2-107[»]
4UK8X-ray3.20X2-107[»]
4UKBX-ray2.90S2-107[»]
4UKEX-ray2.90X2-107[»]
4UKHX-ray3.45S2-107[»]
4UKJX-ray3.45W2-107[»]
4UKMX-ray3.20S2-107[»]
4UKOX-ray3.20W2-107[»]
4UKRX-ray3.30S2-107[»]
4UKTX-ray3.30X2-107[»]
4UKWX-ray3.10S2-107[»]
4UKYX-ray3.10X2-107[»]
4UL1X-ray3.60S2-107[»]
4UL3X-ray3.60X2-107[»]
4UL6X-ray3.00S2-107[»]
4UL8X-ray3.00X2-107[»]
4ULBX-ray3.00S2-107[»]
4ULDX-ray3.00X2-107[»]
4ULGX-ray3.20S2-107[»]
4ULIX-ray3.20X2-107[»]
4ULMX-ray3.10S2-107[»]
4ULOX-ray3.10X2-107[»]
4ULRX-ray3.20S2-107[»]
4ULTX-ray3.20W2-107[»]
4V7Felectron microscopy8.70e1-107[»]
4V8Telectron microscopy8.10f1-107[»]
ProteinModelPortaliP05744.
SMRiP05744. Positions 2-107.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36040. 48 interactions.
IntActiP05744. 14 interactions.
MINTiMINT-8285485.
STRINGi4932.YPL143W.

Proteomic databases

MaxQBiP05744.
PeptideAtlasiP05744.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPL143W; YPL143W; YPL143W.
GeneIDi855960.
KEGGisce:YPL143W.

Organism-specific databases

SGDiS000006064. RPL33A.

Phylogenomic databases

GeneTreeiENSGT00390000016972.
HOGENOMiHOG000195636.
InParanoidiP05744.
KOiK02917.
OMAiISYQRSK.
OrthoDBiEOG7DFXRH.

Enzyme and pathway databases

BioCyciYEAST:G3O-34040-MONOMER.
ReactomeiREACT_188965. SRP-dependent cotranslational protein targeting to membrane.
REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
REACT_217188. Formation of a pool of free 40S subunits.
REACT_232946. GTP hydrolysis and joining of the 60S ribosomal subunit.
REACT_252688. L13a-mediated translational silencing of Ceruloplasmin expression.
REACT_257951. Peptide chain elongation.

Miscellaneous databases

NextBioi980759.

Gene expression databases

GenevestigatoriP05744.

Family and domain databases

InterProiIPR001780. Ribosomal_L35A.
IPR018266. Ribosomal_L35Ae_CS.
IPR009000. Transl_B-barrel.
[Graphical view]
PANTHERiPTHR10902. PTHR10902. 1 hit.
PfamiPF01247. Ribosomal_L35Ae. 1 hit.
[Graphical view]
ProDomiPD012670. Ribosomal_L35Ae. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF50447. SSF50447. 1 hit.
PROSITEiPS01105. RIBOSOMAL_L35AE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Screening a yeast promoter library leads to the isolation of the RP29/L32 and SNR17B/RPL37A divergent promoters and the discovery of a gene encoding ribosomal protein L37."
    Santangelo G.M., Tornow J., McLaughlin C.S., Moldave K.
    Gene 105:137-138(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204626 / S288c / A364A.
  2. "The sequence of 55 kb on the left arm of yeast chromosome XVI identifies a small nuclear RNA, a new putative protein kinase and two new putative regulators."
    Purnelle B., Coster F., Goffeau A.
    Yeast 12:1483-1492(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204511 / S288c / AB972.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Yeast ribosomal proteins. VIII. Isolation of two proteins and sequence characterization of twenty-four proteins from cytoplasmic ribosomes."
    Otaka E., Higo K., Itoh T.
    Mol. Gen. Genet. 195:544-546(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL PROTEIN SEQUENCE OF 2-26, CLEAVAGE OF INITIATOR METHIONINE.
  6. "The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae."
    Planta R.J., Mager W.H.
    Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NOMENCLATURE, SUBUNIT.
  7. "The action of N-terminal acetyltransferases on yeast ribosomal proteins."
    Arnold R.J., Polevoda B., Reilly J.P., Sherman F.
    J. Biol. Chem. 274:37035-37040(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT ALA-2 BY NATA.
  8. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
    Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
    Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Crystal structure of the eukaryotic ribosome."
    Ben-Shem A., Jenner L., Yusupova G., Yusupov M.
    Science 330:1203-1209(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (4.0 ANGSTROMS) OF 80S RIBOSOME.
  10. Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF 80S RIBOSOME.

Entry informationi

Entry nameiRL33A_YEAST
AccessioniPrimary (citable) accession number: P05744
Secondary accession number(s): D6W3M6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: January 23, 2007
Last modified: February 4, 2015
This is version 132 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

There are 2 genes for L33 in yeast.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.