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P05744

- RL33A_YEAST

UniProt

P05744 - RL33A_YEAST

Protein

60S ribosomal protein L33-A

Gene

RPL33A

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
  1. Functioni

    GO - Molecular functioni

    1. structural constituent of ribosome Source: SGD

    GO - Biological processi

    1. cytoplasmic translation Source: SGD

    Keywords - Molecular functioni

    Ribonucleoprotein, Ribosomal protein

    Enzyme and pathway databases

    BioCyciYEAST:G3O-34040-MONOMER.
    ReactomeiREACT_188965. SRP-dependent cotranslational protein targeting to membrane.
    REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
    REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
    REACT_217188. Formation of a pool of free 40S subunits.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    60S ribosomal protein L33-A
    Alternative name(s):
    L37
    RP47
    YL37
    Gene namesi
    Name:RPL33A
    Synonyms:RPL37A
    Ordered Locus Names:YPL143W
    ORF Names:LPI4W, P2625
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XVI

    Organism-specific databases

    SGDiS000006064. RPL33A.

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytosolic large ribosomal subunit Source: SGD

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed2 Publications
    Chaini2 – 10710660S ribosomal protein L33-APRO_0000192807Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanine; partial1 Publication

    Post-translational modificationi

    N-terminally acetylated by acetyltransferase NatA.1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiP05744.
    PeptideAtlasiP05744.

    Expressioni

    Gene expression databases

    GenevestigatoriP05744.

    Interactioni

    Subunit structurei

    Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S).1 Publication

    Protein-protein interaction databases

    BioGridi36040. 47 interactions.
    IntActiP05744. 14 interactions.
    MINTiMINT-8285485.
    STRINGi4932.YPL143W.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1VW8electron microscopy6.10g1-107[»]
    1VWUelectron microscopy6.10g1-107[»]
    3IZSelectron microscopy-j1-107[»]
    3J65electron microscopy8.70e1-107[»]
    3U5EX-ray3.00f1-107[»]
    3U5IX-ray3.00f1-107[»]
    4B6Aelectron microscopy8.10f1-107[»]
    4BYNelectron microscopy4.30f2-107[»]
    4BYUelectron microscopy6.60f2-107[»]
    4CUWelectron microscopy3.70f1-107[»]
    ProteinModelPortaliP05744.
    SMRiP05744. Positions 2-107.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ribosomal protein L35Ae family.Curated

    Phylogenomic databases

    GeneTreeiENSGT00390000016972.
    HOGENOMiHOG000195636.
    KOiK02917.
    OMAiSTRLYSK.
    OrthoDBiEOG7DFXRH.

    Family and domain databases

    InterProiIPR001780. Ribosomal_L35A.
    IPR018266. Ribosomal_L35Ae_CS.
    IPR009000. Transl_B-barrel.
    [Graphical view]
    PANTHERiPTHR10902. PTHR10902. 1 hit.
    PfamiPF01247. Ribosomal_L35Ae. 1 hit.
    [Graphical view]
    ProDomiPD012670. Ribosomal_L35Ae. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SUPFAMiSSF50447. SSF50447. 1 hit.
    PROSITEiPS01105. RIBOSOMAL_L35AE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P05744-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAESHRLYVK GKHLSYQRSK RVNNPNVSLI KIEGVATPQD AQFYLGKRIA    50
    YVYRASKEVR GSKIRVMWGK VTRTHGNSGV VRATFRNNLP AKTFGASVRI 100
    FLYPSNI 107
    Length:107
    Mass (Da):12,154
    Last modified:January 23, 2007 - v3
    Checksum:i263E8389218B5027
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti24 – 241N → D AA sequence (PubMed:18782943)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X57969 Genomic DNA. Translation: CAA41035.1.
    U43703 Genomic DNA. Translation: AAB68218.1.
    Z73499 Genomic DNA. Translation: CAA97847.1.
    BK006949 Genomic DNA. Translation: DAA11292.1.
    PIRiS18431.
    RefSeqiNP_015182.1. NM_001183957.1.

    Genome annotation databases

    EnsemblFungiiYPL143W; YPL143W; YPL143W.
    GeneIDi855960.
    KEGGisce:YPL143W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X57969 Genomic DNA. Translation: CAA41035.1 .
    U43703 Genomic DNA. Translation: AAB68218.1 .
    Z73499 Genomic DNA. Translation: CAA97847.1 .
    BK006949 Genomic DNA. Translation: DAA11292.1 .
    PIRi S18431.
    RefSeqi NP_015182.1. NM_001183957.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1VW8 electron microscopy 6.10 g 1-107 [» ]
    1VWU electron microscopy 6.10 g 1-107 [» ]
    3IZS electron microscopy - j 1-107 [» ]
    3J65 electron microscopy 8.70 e 1-107 [» ]
    3U5E X-ray 3.00 f 1-107 [» ]
    3U5I X-ray 3.00 f 1-107 [» ]
    4B6A electron microscopy 8.10 f 1-107 [» ]
    4BYN electron microscopy 4.30 f 2-107 [» ]
    4BYU electron microscopy 6.60 f 2-107 [» ]
    4CUW electron microscopy 3.70 f 1-107 [» ]
    ProteinModelPortali P05744.
    SMRi P05744. Positions 2-107.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36040. 47 interactions.
    IntActi P05744. 14 interactions.
    MINTi MINT-8285485.
    STRINGi 4932.YPL143W.

    Proteomic databases

    MaxQBi P05744.
    PeptideAtlasi P05744.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YPL143W ; YPL143W ; YPL143W .
    GeneIDi 855960.
    KEGGi sce:YPL143W.

    Organism-specific databases

    SGDi S000006064. RPL33A.

    Phylogenomic databases

    GeneTreei ENSGT00390000016972.
    HOGENOMi HOG000195636.
    KOi K02917.
    OMAi STRLYSK.
    OrthoDBi EOG7DFXRH.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-34040-MONOMER.
    Reactomei REACT_188965. SRP-dependent cotranslational protein targeting to membrane.
    REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
    REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
    REACT_217188. Formation of a pool of free 40S subunits.

    Miscellaneous databases

    NextBioi 980759.

    Gene expression databases

    Genevestigatori P05744.

    Family and domain databases

    InterProi IPR001780. Ribosomal_L35A.
    IPR018266. Ribosomal_L35Ae_CS.
    IPR009000. Transl_B-barrel.
    [Graphical view ]
    PANTHERi PTHR10902. PTHR10902. 1 hit.
    Pfami PF01247. Ribosomal_L35Ae. 1 hit.
    [Graphical view ]
    ProDomi PD012670. Ribosomal_L35Ae. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SUPFAMi SSF50447. SSF50447. 1 hit.
    PROSITEi PS01105. RIBOSOMAL_L35AE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Screening a yeast promoter library leads to the isolation of the RP29/L32 and SNR17B/RPL37A divergent promoters and the discovery of a gene encoding ribosomal protein L37."
      Santangelo G.M., Tornow J., McLaughlin C.S., Moldave K.
      Gene 105:137-138(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204626 / S288c / A364A.
    2. "The sequence of 55 kb on the left arm of yeast chromosome XVI identifies a small nuclear RNA, a new putative protein kinase and two new putative regulators."
      Purnelle B., Coster F., Goffeau A.
      Yeast 12:1483-1492(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204511 / S288c / AB972.
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
      Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
      , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
      Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "Yeast ribosomal proteins. VIII. Isolation of two proteins and sequence characterization of twenty-four proteins from cytoplasmic ribosomes."
      Otaka E., Higo K., Itoh T.
      Mol. Gen. Genet. 195:544-546(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: PARTIAL PROTEIN SEQUENCE OF 2-26, CLEAVAGE OF INITIATOR METHIONINE.
    6. "The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae."
      Planta R.J., Mager W.H.
      Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NOMENCLATURE, SUBUNIT.
    7. "The action of N-terminal acetyltransferases on yeast ribosomal proteins."
      Arnold R.J., Polevoda B., Reilly J.P., Sherman F.
      J. Biol. Chem. 274:37035-37040(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT ALA-2 BY NATA.
    8. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
      Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
      Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "Crystal structure of the eukaryotic ribosome."
      Ben-Shem A., Jenner L., Yusupova G., Yusupov M.
      Science 330:1203-1209(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (4.0 ANGSTROMS) OF 80S RIBOSOME.
    10. Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF 80S RIBOSOME.

    Entry informationi

    Entry nameiRL33A_YEAST
    AccessioniPrimary (citable) accession number: P05744
    Secondary accession number(s): D6W3M6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1988
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 128 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    There are 2 genes for L33 in yeast.

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Ribosomal proteins
      Ribosomal proteins families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families
    4. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    5. Yeast chromosome XVI
      Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

    External Data

    Dasty 3