P05744 (RL33A_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
60S ribosomal protein L33-A

Alternative name(s):
L37
RP47
YL37

Gene names

Name:	RPL33A
Synonyms:	RPL37A
Ordered Locus Names:	YPL143W
ORF Names:	LPI4W, P2625

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	107 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). Ref.6
Subcellular location	Cytoplasm By similarity.
Post-translational modification	N-terminally acetylated by acetyltransferase NatA.
Miscellaneous	There are 2 genes for L33 in yeast.
Sequence similarities	Belongs to the ribosomal protein L35Ae family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Ribonucleoprotein Ribosomal protein
PTM	Acetylation
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cytoplasmic translation Inferred from direct assay PubMed 18782943. Source: SGD
Cellular_component	cytosolic large ribosomal subunit Inferred from direct assay PubMed 18782943. Source: SGD
Molecular_function	structural constituent of ribosome Inferred from direct assay PubMed 18782943. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.5 Ref.7
	Chain	2 – 107	106	60S ribosomal protein L33-A		PRO_0000192807
Amino acid modifications
	Modified residue	2	1	N-acetylalanine; partial Ref.7
Experimental info
	Sequence conflict	24	1	N → D AA sequence Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P05744 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 263E8389218B5027	FASTA	107	12,154
	10 20 30 40 50 60 MAESHRLYVK GKHLSYQRSK RVNNPNVSLI KIEGVATPQD AQFYLGKRIA YVYRASKEVR 70 80 90 100 GSKIRVMWGK VTRTHGNSGV VRATFRNNLP AKTFGASVRI FLYPSNI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Screening a yeast promoter library leads to the isolation of the RP29/L32 and SNR17B/RPL37A divergent promoters and the discovery of a gene encoding ribosomal protein L37." Santangelo G.M., Tornow J., McLaughlin C.S., Moldave K. Gene 105:137-138(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204626 / S288c / A364A.
[2]	"The sequence of 55 kb on the left arm of yeast chromosome XVI identifies a small nuclear RNA, a new putative protein kinase and two new putative regulators." Purnelle B., Coster F., Goffeau A. Yeast 12:1483-1492(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J. Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Yeast ribosomal proteins. VIII. Isolation of two proteins and sequence characterization of twenty-four proteins from cytoplasmic ribosomes." Otaka E., Higo K., Itoh T. Mol. Gen. Genet. 195:544-546(1984) Cited for: PROTEIN SEQUENCE OF 2-26.
[6]	"The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae." Planta R.J., Mager W.H. Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NOMENCLATURE, SUBUNIT.
[7]	"The action of N-terminal acetyltransferases on yeast ribosomal proteins." Arnold R.J., Polevoda B., Reilly J.P., Sherman F. J. Biol. Chem. 274:37035-37040(1999) [PubMed] [Europe PMC] [Abstract] Cited for: CLEAVAGE OF INITIATOR METHIONINE, PARTIAL ACETYLATION AT ALA-2 BY NATA.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X57969 Genomic DNA. Translation: CAA41035.1.
U43703 Genomic DNA. Translation: AAB68218.1.
Z73499 Genomic DNA. Translation: CAA97847.1.
BK006949 Genomic DNA. Translation: DAA11292.1.

PIR

S18431.

RefSeq

NP_015182.1. NM_001183957.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3IZS	electron microscopy	-	j	1-107	[»]
3U5E	X-ray	3.00	f	1-107	[»]
3U5I	X-ray	3.00	f	1-107	[»]
4B6A	electron microscopy	8.10	f	1-107	[»]

ProteinModelPortal

P05744.

SMR

P05744. Positions 2-107.

ModBase

Search...

Protein-protein interaction databases

IntAct

P05744. 11 interactions.

STRING

4932.YPL143W.

Proteomic databases

PeptideAtlas

P05744.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YPL143W; YPL143W; YPL143W.

GeneID

855960.

KEGG

sce:YPL143W.

Organism-specific databases

SGD

S000006064. RPL33A.

Phylogenomic databases

GeneTree

ENSGT00390000016972.

HOGENOM

HOG000195636.

KO

K02917.

OMA

ISYQRSK.

OrthoDB

EOG4BP4NF.

Gene expression databases

Genevestigator

P05744.

GermOnline

YPL143W. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR001780. Ribosomal_L35A.
IPR018266. Ribosomal_L35Ae_CS.
IPR009000. Transl_elong_init/rib_B-barrel.
[Graphical view]

PANTHER

PTHR10902. PTHR10902. 1 hit.

Pfam

PF01247. Ribosomal_L35Ae. 1 hit.
[Graphical view]

ProDom

PD012670. Ribosomal_L35Ae. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SUPFAM

SSF50447. Translat_factor. 1 hit.

PROSITE

PS01105. RIBOSOMAL_L35AE. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

980759.

Entry information

Entry name

RL33A_YEAST

Accession

Primary (citable) accession number: P05744
Secondary accession number(s): D6W3M6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1988
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 116 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program