Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

60S ribosomal protein L33-A

Gene

RPL33A

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel.1 Publication

Miscellaneous

There are 2 genes for eL33 in yeast.Curated

GO - Molecular functioni

  • structural constituent of ribosome Source: SGD

GO - Biological processi

  • cytoplasmic translation Source: SGD
  • ribosomal large subunit biogenesis Source: GO_Central

Keywordsi

Molecular functionRibonucleoprotein, Ribosomal protein

Enzyme and pathway databases

BioCyciYEAST:G3O-34040-MONOMER
ReactomeiR-SCE-156827 L13a-mediated translational silencing of Ceruloplasmin expression
R-SCE-1799339 SRP-dependent cotranslational protein targeting to membrane
R-SCE-72689 Formation of a pool of free 40S subunits
R-SCE-72706 GTP hydrolysis and joining of the 60S ribosomal subunit
R-SCE-975956 Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC)
R-SCE-975957 Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC)

Names & Taxonomyi

Protein namesi
Recommended name:
60S ribosomal protein L33-A1 Publication
Alternative name(s):
L37
Large ribosomal subunit protein eL33-A1 Publication
RP47
YL37
Gene namesi
Name:RPL33A1 Publication
Synonyms:RPL37A
Ordered Locus Names:YPL143W
ORF Names:LPI4W, P2625
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL143W
SGDiS000006064 RPL33A

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved2 Publications
ChainiPRO_00001928072 – 10760S ribosomal protein L33-AAdd BLAST106

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanine; partial1 Publication1
Cross-linki47Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources

Post-translational modificationi

N-terminally acetylated by acetyltransferase NatA.1 Publication

Keywords - PTMi

Acetylation, Isopeptide bond, Ubl conjugation

Proteomic databases

MaxQBiP05744
PaxDbiP05744
PRIDEiP05744
TopDownProteomicsiP05744

PTM databases

iPTMnetiP05744

Interactioni

Subunit structurei

Component of the large ribosomal subunit (LSU). Mature yeast ribosomes consist of a small (40S) and a large (60S) subunit. The 40S small subunit contains 1 molecule of ribosomal RNA (18S rRNA) and 33 different proteins (encoded by 57 genes). The large 60S subunit contains 3 rRNA molecules (25S, 5.8S and 5S rRNA) and 46 different proteins (encoded by 81 genes) (PubMed:9559554, PubMed:22096102).1 Publication1 Publication

Protein-protein interaction databases

BioGridi36040, 111 interactors
IntActiP05744, 15 interactors
MINTiP05744
STRINGi4932.YPL143W

Structurei

Secondary structure

1107
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi8 – 18Combined sources11
Beta strandi23 – 30Combined sources8
Helixi38 – 41Combined sources4
Helixi42 – 44Combined sources3
Beta strandi48 – 54Combined sources7
Beta strandi59 – 61Combined sources3
Beta strandi63 – 74Combined sources12
Beta strandi81 – 87Combined sources7
Turni92 – 95Combined sources4
Beta strandi96 – 101Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3J6Xelectron microscopy6.10731-107[»]
3J6Yelectron microscopy6.10731-107[»]
3J77electron microscopy6.20831-107[»]
3J78electron microscopy6.30831-107[»]
3JCTelectron microscopy3.08f1-107[»]
4U3MX-ray3.00O3/o32-107[»]
4U3NX-ray3.20O3/o32-107[»]
4U3UX-ray2.90O3/o32-107[»]
4U4NX-ray3.10O3/o32-107[»]
4U4OX-ray3.60O3/o32-107[»]
4U4QX-ray3.00O3/o32-107[»]
4U4RX-ray2.80O3/o32-107[»]
4U4UX-ray3.00O3/o32-107[»]
4U4YX-ray3.20O3/o32-107[»]
4U4ZX-ray3.10O3/o32-107[»]
4U50X-ray3.20O3/o32-107[»]
4U51X-ray3.20O3/o32-107[»]
4U52X-ray3.00O3/o32-107[»]
4U53X-ray3.30O3/o32-107[»]
4U55X-ray3.20O3/o32-107[»]
4U56X-ray3.45O3/o32-107[»]
4U6FX-ray3.10O3/o32-107[»]
4V6Ielectron microscopy8.80Bj1-107[»]
4V7Felectron microscopy8.70e1-107[»]
4V88X-ray3.00Bf/Df1-107[»]
4V8Telectron microscopy8.10f1-107[»]
4V8Yelectron microscopy4.30Bf2-107[»]
4V8Zelectron microscopy6.60Bf2-107[»]
4V91electron microscopy3.70f1-107[»]
5APNelectron microscopy3.91f1-107[»]
5APOelectron microscopy3.41f1-107[»]
5DATX-ray3.15O3/o32-107[»]
5DC3X-ray3.25O3/o32-107[»]
5DGEX-ray3.45O3/o32-107[»]
5DGFX-ray3.30O3/o32-107[»]
5DGVX-ray3.10O3/o32-107[»]
5FCIX-ray3.40O3/o32-107[»]
5FCJX-ray3.10O3/o32-107[»]
5FL8electron microscopy9.50f1-107[»]
5GAKelectron microscopy3.88h1-107[»]
5H4Pelectron microscopy3.07f1-107[»]
5I4LX-ray3.10O3/o32-107[»]
5JCSelectron microscopy9.50f1-107[»]
5JUOelectron microscopy4.00KA1-107[»]
5JUPelectron microscopy3.50KA1-107[»]
5JUSelectron microscopy4.20KA1-107[»]
5JUTelectron microscopy4.00KA1-107[»]
5JUUelectron microscopy4.00KA1-107[»]
5LYBX-ray3.25O3/o32-107[»]
5M1Jelectron microscopy3.30f52-107[»]
5MC6electron microscopy3.80BK1-107[»]
5MEIX-ray3.50AG/DH2-107[»]
5NDGX-ray3.70O3/o32-107[»]
5NDVX-ray3.30O3/o32-107[»]
5NDWX-ray3.70O3/o32-107[»]
5OBMX-ray3.40O3/o32-107[»]
5ON6X-ray3.10AG/DH2-107[»]
5T62electron microscopy3.30s1-107[»]
5T6Relectron microscopy4.50s1-107[»]
5TBWX-ray3.00AG/DH2-107[»]
5TGAX-ray3.30O3/o32-107[»]
5TGMX-ray3.50O3/o32-107[»]
6C0Felectron microscopy3.70f1-107[»]
6CB1electron microscopy4.60f1-107[»]
6ELZelectron microscopy3.30f1-107[»]
6EM1electron microscopy3.60f1-107[»]
6EM3electron microscopy3.20f1-107[»]
6EM4electron microscopy4.10f1-107[»]
6EM5electron microscopy4.30f1-107[»]
ProteinModelPortaliP05744
SMRiP05744
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000016972
HOGENOMiHOG000195636
InParanoidiP05744
KOiK02917
OMAiVRAIWGK
OrthoDBiEOG092C5MJS

Family and domain databases

Gene3Di2.40.10.190, 1 hit
HAMAPiMF_00573 Ribosomal_L35Ae, 1 hit
InterProiView protein in InterPro
IPR038661 L35A_sf
IPR001780 Ribosomal_L35A
IPR018266 Ribosomal_L35Ae_CS
IPR009000 Transl_B-barrel_sf
PANTHERiPTHR10902 PTHR10902, 1 hit
PfamiView protein in Pfam
PF01247 Ribosomal_L35Ae, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD012670 Ribosomal_L35Ae, 1 hit
SUPFAMiSSF50447 SSF50447, 1 hit
PROSITEiView protein in PROSITE
PS01105 RIBOSOMAL_L35AE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P05744-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAESHRLYVK GKHLSYQRSK RVNNPNVSLI KIEGVATPQD AQFYLGKRIA
60 70 80 90 100
YVYRASKEVR GSKIRVMWGK VTRTHGNSGV VRATFRNNLP AKTFGASVRI

FLYPSNI
Length:107
Mass (Da):12,154
Last modified:January 23, 2007 - v3
Checksum:i263E8389218B5027
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti24N → D AA sequence (PubMed:18782943).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X57969 Genomic DNA Translation: CAA41035.1
U43703 Genomic DNA Translation: AAB68218.1
Z73499 Genomic DNA Translation: CAA97847.1
BK006949 Genomic DNA Translation: DAA11292.1
PIRiS18431
RefSeqiNP_015182.1, NM_001183957.1

Genome annotation databases

EnsemblFungiiYPL143W; YPL143W; YPL143W
GeneIDi855960
KEGGisce:YPL143W

Similar proteinsi

Entry informationi

Entry nameiRL33A_YEAST
AccessioniPrimary (citable) accession number: P05744
Secondary accession number(s): D6W3M6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: January 23, 2007
Last modified: April 25, 2018
This is version 164 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health