Reviewed, UniProtKB/Swiss-Prot P05740 (RL17A_YEAST)

Names and origin

Protein names

Recommended name:
    60S ribosomal protein L17-A
Alternative name(s):
    L20A
    YL17

Gene names

Name:	RPL17A
Synonyms:	RPL17, RPL20A
Ordered Locus Names:	YKL180W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	184 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Subunit structure	Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). Ref.4
Subcellular location	Cytoplasm Ref.6.
Miscellaneous	There are 2 genes for L17 in yeast.
Sequence similarities	Belongs to the ribosomal protein L22P family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Ribonucleoprotein Ribosomal protein
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	translation Inferred from direct assay. Source: SGD
Cellular component	cytosolic large ribosomal subunit Inferred from direct assay. Source: SGD
Molecular function	structural constituent of ribosome Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.3 Ref.5
	Chain	2 – 184	183	60S ribosomal protein L17-A		PRO_0000125347
Amino acid modifications
	Modified residue	14	1	Phosphoserine Ref.8
	Modified residue	65	1	Phosphoserine Ref.8 Ref.7
	Modified residue	66	1	Phosphoserine Ref.8
Experimental info
	Sequence conflict	104 – 114	11	AKGLDATKLYV → VCQEYYMFSTR Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P05740-1 [UniParc]. Last modified January 23, 2007. Version 4. Checksum: B8902DD90714671D	FASTA	184	20,549
	10 20 30 40 50 60 MARYGATSTN PAKSASARGS YLRVSFKNTR ETAQAINGWE LTKAQKYLEQ VLDHQRAIPF 70 80 90 100 110 120 RRFNSSIGRT AQGKEFGVTK ARWPAKSVKF VQGLLQNAAA NAEAKGLDAT KLYVSHIQVN 130 140 150 160 170 180 QAPKQRRRTY RAHGRINKYE SSPSHIELVV TEKEEAVAKA AEKKVVRLTS RQRGRIAAQK RIAA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed: 8196765] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"Sequencing and analysis of 51.6 kilobases on the left arm of chromosome XI from Saccharomyces cerevisiae reveals 23 open reading frames including the FAS1 gene." Wiemann S., Voss H., Schwager C., Rupp T., Stegemann J., Zimmermann J., Grothues D., Sensen C., Erfle H., Hewitt N., Banrevi A., Ansorge W. Yeast 9:1343-1348(1993) [PubMed: 8154185] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-114.
[3]	"Yeast ribosomal proteins. VIII. Isolation of two proteins and sequence characterization of twenty-four proteins from cytoplasmic ribosomes." Otaka E., Higo K., Itoh T. Mol. Gen. Genet. 195:544-546(1984) Cited for: PRELIMINARY PROTEIN SEQUENCE OF 2-41.
[4]	"The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae." Planta R.J., Mager W.H. Yeast 14:471-477(1998) [PubMed: 9559554] [Abstract] Cited for: NOMENCLATURE, SUBUNIT.
[5]	"The action of N-terminal acetyltransferases on yeast ribosomal proteins." Arnold R.J., Polevoda B., Reilly J.P., Sherman F. J. Biol. Chem. 274:37035-37040(1999) [PubMed: 10601260] [Abstract] Cited for: CLEAVAGE OF INITIATOR METHIONINE.
[6]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed: 15665377] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-65, MASS SPECTROMETRY.
[8]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-14; SER-65 AND SER-66, MASS SPECTROMETRY.
[9]	"Structure of the 80S ribosome from Saccharomyces cerevisiae -- tRNA-ribosome and subunit-subunit interactions." Spahn C.M.T., Beckmann R., Eswar N., Penczek P.A., Sali A., Blobel G., Frank J. Cell 107:373-386(2001) [PubMed: 11701127] [Abstract] Cited for: 3D-STRUCTURE MODELING OF 6-152, ELECTRON MICROSCOPY.
[10]	"Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation." Spahn C.M.T., Gomez-Lorenzo M.G., Grassucci R.A., Joergensen R., Andersen G.R., Beckmann R., Penczek P.A., Ballesta J.P.G., Frank J. EMBO J. 23:1008-1019(2004) [PubMed: 14976550] [Abstract] Cited for: 3D-STRUCTURE MODELING, ELECTRON MICROSCOPY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

Z28180 Genomic DNA. Translation: CAA82023.1.
Z28179 Genomic DNA. Translation: CAA82022.1.
X74151 Genomic DNA. Translation: CAA52258.1.

PIR

S38012.

RefSeq

NP_012741.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1K5Y	model	-	N	6-152	[»]
1S1I	electron microscopy	11.70	N	2-184	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-5529N.

IntAct

P05740. 57 interactions.

STRING

P05740.

Proteomic databases

PeptideAtlas

P05740.

PRIDE

P05740.

Genome annotation databases

Ensembl

YKL180W; YKL180W; YKL180W; Saccharomyces cerevisiae. [Genome view]

GeneID

853674.

KEGG

sce:YKL180W.

NMPDR

fig|4932.3.peg.3719.

Organism-specific databases

CYGD

YKL180w.

SGD

S000001663. RPL17A.

Phylogenomic databases

HOGENOM

HBG568696.

OMA

GWKLERA.

OrthoDB

EOG9Q2FZ3.

Gene expression databases

ArrayExpress

P05740.

Genevestigator

P05740.

GermOnline

YKL180W. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR001063. Ribosomal_L22.
IPR018260. Ribosomal_L22/L17_CS.
IPR005721. Ribosomal_L22/L17_euk/arc.
[Graphical view]

PANTHER

PTHR11593. Ribosomal_L22/17. 1 hit.

Pfam

PF00237. Ribosomal_L22. 1 hit.
[Graphical view]

PROSITE

PS00464. RIBOSOMAL_L22. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

974625.

Entry information

Entry name

RL17A_YEAST

Accession

Primary (citable) accession number: P05740

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1988
Last sequence update:	January 23, 2007
Last modified:	December 15, 2009
This is version 94 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)