60S ribosomal protein L9-A - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Contribute

Send feedback

Read comments (?) or add your own

P05738 (RL9A_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 125. History...

Clusters with 100%, 90%, 50% identity |

Documents (5) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
60S ribosomal protein L9-A

Alternative name(s):
L8
RP24
YL11

Gene names

Name:	RPL9A
Synonyms:	RPL8A, RPL9
Ordered Locus Names:	YGL147C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	191 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). Ref.6
Subcellular location	Cytoplasm Ref.7.
Miscellaneous	Present with 52400 molecules/cell in log phase SD medium. There are 2 genes for L9 in yeast.
Sequence similarities	Belongs to the ribosomal protein L6P family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cytoplasmic translation Inferred from direct assay PubMed 18782943. Source: SGD
Cellular_component	cytosolic large ribosomal subunit Inferred from direct assay PubMed 18782943. Source: SGD
Molecular_function	rRNA binding Inferred from electronic annotation. Source: InterPro structural constituent of ribosome Inferred from direct assay PubMed 18782943. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 191

191

60S ribosomal protein L9-A

PRO_0000131111

Sequences

Sequence

Length

Mass (Da)

Tools

P05738 [UniParc].

Last modified December 1, 1992. Version 2.
Checksum: CAA342FCDD061175
Show »

FASTA

191

21,569

        10         20         30         40         50         60 
MKYIQTEQQI EVPEGVTVSI KSRIVKVVGP RGTLTKNLKH IDVTFTKVNN QLIKVAVHNG 

        70         80         90        100        110        120 
GRKHVAALRT VKSLVDNMIT GVTKGYKYKM RYVYAHFPIN VNIVEKDGAK FIEVRNFLGD 

       130        140        150        160        170        180 
KKIRNVPVRD GVTIEFSTNV KDEIVLSGNS VEDVSQNAAD LQQICRVRNK DIRKFLDGIY 

       190 
VSHKGFITED L

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterisation of a yeast homolog of the mammalian ribosomal protein L9." Jones D.G.L., Reusser U., Braus G.H. Nucleic Acids Res. 19:5785-5785(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The sequence of a nearly unclonable 22.8 kb segment on the left arm chromosome VII from Saccharomyces cerevisiae reveals ARO2, RPL9A, TIP1, MRF1 genes and six new open reading frames." Voet M., Defoor E., Verhasselt P., Riles L., Robben J., Volckaert G. Yeast 13:177-182(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. Kleine K. Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Yeast ribosomal proteins. VIII. Isolation of two proteins and sequence characterization of twenty-four proteins from cytoplasmic ribosomes." Otaka E., Higo K., Itoh T. Mol. Gen. Genet. 195:544-546(1984) Cited for: PRELIMINARY PROTEIN SEQUENCE OF 1-40.
[6]	"The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae." Planta R.J., Mager W.H. Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NOMENCLATURE, SUBUNIT.
[7]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"Structure of the 80S ribosome from Saccharomyces cerevisiae -- tRNA-ribosome and subunit-subunit interactions." Spahn C.M.T., Beckmann R., Eswar N., Penczek P.A., Sali A., Blobel G., Frank J. Cell 107:373-386(2001) [PubMed] [Europe PMC] [Abstract] Cited for: 3D-STRUCTURE MODELING OF 7-184, ELECTRON MICROSCOPY.
[10]	"Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation." Spahn C.M.T., Gomez-Lorenzo M.G., Grassucci R.A., Joergensen R., Andersen G.R., Beckmann R., Penczek P.A., Ballesta J.P.G., Frank J. EMBO J. 23:1008-1019(2004) [PubMed] [Europe PMC] [Abstract] Cited for: 3D-STRUCTURE MODELING, ELECTRON MICROSCOPY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X60190 Genomic DNA. Translation: CAA42746.1.
X99960 Genomic DNA. Translation: CAA68215.1.
Z72669 Genomic DNA. Translation: CAA96859.1.
BK006941 Genomic DNA. Translation: DAA07963.1.

PIR

R5BYL9. S19077.

RefSeq

NP_011368.3. NM_001181012.3.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1K5Y	model	-	H	7-184	[»]
1S1I	electron microscopy	11.70	H	1-191	[»]
3IZS	electron microscopy	-	F	1-191	[»]
3J16	electron microscopy	-	F	1-191	[»]
3O58	X-ray	4.00	I	1-191	[»]
3O5H	X-ray	4.00	I	1-191	[»]
3U5E	X-ray	3.00	H	1-191	[»]
3U5I	X-ray	3.00	H	1-191	[»]
4B6A	electron microscopy	8.10	H	1-191	[»]

ProteinModelPortal

P05738.

SMR

P05738. Positions 1-191.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-4826N.

IntAct

P05738. 7 interactions.

MINT

MINT-493534.

STRING

4932.YGL147C.

Proteomic databases

PeptideAtlas

P05738.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YGL147C; YGL147C; YGL147C.

GeneID

852730.

KEGG

sce:YGL143C.
sce:YGL147C.

Organism-specific databases

CYGD

YGL147c.

SGD

S000003115. RPL9A.

Phylogenomic databases

GeneTree

ENSGT00390000015224.

HOGENOM

HOG000039905.

K02835.
K02940.

OMA

RDTRIFQ.

OrthoDB

EOG4RJK9X.

Gene expression databases

Genevestigator

P05738.

GermOnline

YGL147C. Saccharomyces cerevisiae.

Family and domain databases

Gene3D

3.90.930.12. 2 hits.

InterPro

IPR000702. Ribosomal_L6.
IPR020040. Ribosomal_L6_a/b-dom.
IPR002359. Ribosomal_L6_CS2.
[Graphical view]

PANTHER

PTHR11655. PTHR11655. 1 hit.

Pfam

PF00347. Ribosomal_L6. 2 hits.
[Graphical view]

PIRSF

PIRSF002162. Ribosomal_L6. 1 hit.

SUPFAM

SSF56053. Ribosomal_L6. 2 hits.

PROSITE

PS00700. RIBOSOMAL_L6_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P05738.

NextBio

972123.

Entry information

Entry name

RL9A_YEAST

Accession

Primary (citable) accession number: P05738
Secondary accession number(s): D6VU02

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1988
Last sequence update:	December 1, 1992
Last modified:	April 3, 2013
This is version 125 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program