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Protein

60S ribosomal protein L9-A

Gene

RPL9A

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

  1. rRNA binding Source: InterPro
  2. structural constituent of ribosome Source: SGD

GO - Biological processi

  1. cytoplasmic translation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Enzyme and pathway databases

BioCyciYEAST:G3O-30641-MONOMER.
ReactomeiREACT_188965. SRP-dependent cotranslational protein targeting to membrane.
REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
REACT_217188. Formation of a pool of free 40S subunits.
REACT_232946. GTP hydrolysis and joining of the 60S ribosomal subunit.
REACT_252688. L13a-mediated translational silencing of Ceruloplasmin expression.
REACT_257951. Peptide chain elongation.

Names & Taxonomyi

Protein namesi
Recommended name:
60S ribosomal protein L9-A
Alternative name(s):
L8
RP24
YL11
Gene namesi
Name:RPL9A
Synonyms:RPL8A, RPL9
Ordered Locus Names:YGL147C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VII

Organism-specific databases

CYGDiYGL147c.
SGDiS000003115. RPL9A.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytosolic large ribosomal subunit Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 19119160S ribosomal protein L9-APRO_0000131111Add
BLAST

Proteomic databases

MaxQBiP05738.
PeptideAtlasiP05738.

Expressioni

Gene expression databases

GenevestigatoriP05738.

Interactioni

Subunit structurei

Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S).1 Publication

Protein-protein interaction databases

BioGridi33105. 120 interactions.
DIPiDIP-4826N.
IntActiP05738. 9 interactions.
MINTiMINT-493534.
STRINGi4932.YGL147C.

Structurei

Secondary structure

1
191
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 119Combined sources
Beta strandi17 – 215Combined sources
Beta strandi24 – 296Combined sources
Beta strandi32 – 376Combined sources
Turni39 – 413Combined sources
Beta strandi44 – 496Combined sources
Beta strandi52 – 609Combined sources
Helixi62 – 8322Combined sources
Beta strandi86 – 938Combined sources
Beta strandi95 – 973Combined sources
Beta strandi100 – 1045Combined sources
Beta strandi106 – 1083Combined sources
Beta strandi111 – 1155Combined sources
Helixi117 – 1193Combined sources
Beta strandi124 – 1274Combined sources
Beta strandi132 – 1365Combined sources
Beta strandi138 – 1403Combined sources
Beta strandi143 – 1497Combined sources
Helixi151 – 16313Combined sources
Beta strandi168 – 1703Combined sources
Turni172 – 1743Combined sources
Beta strandi178 – 18912Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1K5Ymodel-H7-184[»]
3J16electron microscopy-F1-191[»]
3J6Xelectron microscopy6.10L91-191[»]
3J6Yelectron microscopy6.10L91-191[»]
3J77electron microscopy6.20L91-191[»]
3J78electron microscopy6.30L91-191[»]
4U3MX-ray3.00L9/l91-191[»]
4U3NX-ray3.20L9/l91-191[»]
4U3UX-ray2.90L9/l91-191[»]
4U4NX-ray3.10L9/l91-191[»]
4U4OX-ray3.60L9/l91-191[»]
4U4QX-ray3.00L9/l91-191[»]
4U4RX-ray2.80L9/l91-191[»]
4U4UX-ray3.00L9/l91-191[»]
4U4YX-ray3.20L9/l91-191[»]
4U4ZX-ray3.10L9/l91-191[»]
4U50X-ray3.20L9/l91-191[»]
4U51X-ray3.20L9/l91-191[»]
4U52X-ray3.00L9/l91-191[»]
4U53X-ray3.30L9/l91-191[»]
4U55X-ray3.20L9/l91-191[»]
4U56X-ray3.45L9/l91-191[»]
4U6FX-ray3.10L9/l91-191[»]
4V4Belectron microscopy11.70BH1-191[»]
4V6Ielectron microscopy8.80BF1-191[»]
4V7Felectron microscopy8.70F1-191[»]
4V7RX-ray4.00BI/DI1-191[»]
4V88X-ray3.00BH/DH1-191[»]
4V8Telectron microscopy8.10H1-191[»]
4V8Yelectron microscopy4.30BH1-191[»]
4V8Zelectron microscopy6.60BH1-191[»]
4V91electron microscopy3.70H1-191[»]
ProteinModelPortaliP05738.
SMRiP05738. Positions 1-191.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP05738.

Family & Domainsi

Sequence similaritiesi

Belongs to the ribosomal protein L6P family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000015224.
HOGENOMiHOG000039905.
InParanoidiP05738.
KOiK02940.
OMAiALDIRMV.
OrthoDBiEOG7FR7TQ.

Family and domain databases

Gene3Di3.90.930.12. 2 hits.
InterProiIPR000702. Ribosomal_L6.
IPR020040. Ribosomal_L6_a/b-dom.
IPR002359. Ribosomal_L6_CS2.
[Graphical view]
PANTHERiPTHR11655. PTHR11655. 1 hit.
PfamiPF00347. Ribosomal_L6. 2 hits.
[Graphical view]
PIRSFiPIRSF002162. Ribosomal_L6. 1 hit.
SUPFAMiSSF56053. SSF56053. 2 hits.
PROSITEiPS00700. RIBOSOMAL_L6_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P05738-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKYIQTEQQI EVPEGVTVSI KSRIVKVVGP RGTLTKNLKH IDVTFTKVNN
60 70 80 90 100
QLIKVAVHNG GRKHVAALRT VKSLVDNMIT GVTKGYKYKM RYVYAHFPIN
110 120 130 140 150
VNIVEKDGAK FIEVRNFLGD KKIRNVPVRD GVTIEFSTNV KDEIVLSGNS
160 170 180 190
VEDVSQNAAD LQQICRVRNK DIRKFLDGIY VSHKGFITED L
Length:191
Mass (Da):21,569
Last modified:December 1, 1992 - v2
Checksum:iCAA342FCDD061175
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X60190 Genomic DNA. Translation: CAA42746.1.
X99960 Genomic DNA. Translation: CAA68215.1.
Z72669 Genomic DNA. Translation: CAA96859.1.
BK006941 Genomic DNA. Translation: DAA07963.1.
PIRiS19077. R5BYL9.
RefSeqiNP_011368.3. NM_001181012.3.

Genome annotation databases

EnsemblFungiiYGL147C; YGL147C; YGL147C.
GeneIDi852730.
KEGGisce:YGL147C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X60190 Genomic DNA. Translation: CAA42746.1.
X99960 Genomic DNA. Translation: CAA68215.1.
Z72669 Genomic DNA. Translation: CAA96859.1.
BK006941 Genomic DNA. Translation: DAA07963.1.
PIRiS19077. R5BYL9.
RefSeqiNP_011368.3. NM_001181012.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1K5Ymodel-H7-184[»]
3J16electron microscopy-F1-191[»]
3J6Xelectron microscopy6.10L91-191[»]
3J6Yelectron microscopy6.10L91-191[»]
3J77electron microscopy6.20L91-191[»]
3J78electron microscopy6.30L91-191[»]
4U3MX-ray3.00L9/l91-191[»]
4U3NX-ray3.20L9/l91-191[»]
4U3UX-ray2.90L9/l91-191[»]
4U4NX-ray3.10L9/l91-191[»]
4U4OX-ray3.60L9/l91-191[»]
4U4QX-ray3.00L9/l91-191[»]
4U4RX-ray2.80L9/l91-191[»]
4U4UX-ray3.00L9/l91-191[»]
4U4YX-ray3.20L9/l91-191[»]
4U4ZX-ray3.10L9/l91-191[»]
4U50X-ray3.20L9/l91-191[»]
4U51X-ray3.20L9/l91-191[»]
4U52X-ray3.00L9/l91-191[»]
4U53X-ray3.30L9/l91-191[»]
4U55X-ray3.20L9/l91-191[»]
4U56X-ray3.45L9/l91-191[»]
4U6FX-ray3.10L9/l91-191[»]
4V4Belectron microscopy11.70BH1-191[»]
4V6Ielectron microscopy8.80BF1-191[»]
4V7Felectron microscopy8.70F1-191[»]
4V7RX-ray4.00BI/DI1-191[»]
4V88X-ray3.00BH/DH1-191[»]
4V8Telectron microscopy8.10H1-191[»]
4V8Yelectron microscopy4.30BH1-191[»]
4V8Zelectron microscopy6.60BH1-191[»]
4V91electron microscopy3.70H1-191[»]
ProteinModelPortaliP05738.
SMRiP05738. Positions 1-191.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33105. 120 interactions.
DIPiDIP-4826N.
IntActiP05738. 9 interactions.
MINTiMINT-493534.
STRINGi4932.YGL147C.

Proteomic databases

MaxQBiP05738.
PeptideAtlasiP05738.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGL147C; YGL147C; YGL147C.
GeneIDi852730.
KEGGisce:YGL147C.

Organism-specific databases

CYGDiYGL147c.
SGDiS000003115. RPL9A.

Phylogenomic databases

GeneTreeiENSGT00390000015224.
HOGENOMiHOG000039905.
InParanoidiP05738.
KOiK02940.
OMAiALDIRMV.
OrthoDBiEOG7FR7TQ.

Enzyme and pathway databases

BioCyciYEAST:G3O-30641-MONOMER.
ReactomeiREACT_188965. SRP-dependent cotranslational protein targeting to membrane.
REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
REACT_217188. Formation of a pool of free 40S subunits.
REACT_232946. GTP hydrolysis and joining of the 60S ribosomal subunit.
REACT_252688. L13a-mediated translational silencing of Ceruloplasmin expression.
REACT_257951. Peptide chain elongation.

Miscellaneous databases

EvolutionaryTraceiP05738.
NextBioi972123.

Gene expression databases

GenevestigatoriP05738.

Family and domain databases

Gene3Di3.90.930.12. 2 hits.
InterProiIPR000702. Ribosomal_L6.
IPR020040. Ribosomal_L6_a/b-dom.
IPR002359. Ribosomal_L6_CS2.
[Graphical view]
PANTHERiPTHR11655. PTHR11655. 1 hit.
PfamiPF00347. Ribosomal_L6. 2 hits.
[Graphical view]
PIRSFiPIRSF002162. Ribosomal_L6. 1 hit.
SUPFAMiSSF56053. SSF56053. 2 hits.
PROSITEiPS00700. RIBOSOMAL_L6_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterisation of a yeast homolog of the mammalian ribosomal protein L9."
    Jones D.G.L., Reusser U., Braus G.H.
    Nucleic Acids Res. 19:5785-5785(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The sequence of a nearly unclonable 22.8 kb segment on the left arm chromosome VII from Saccharomyces cerevisiae reveals ARO2, RPL9A, TIP1, MRF1 genes and six new open reading frames."
    Voet M., Defoor E., Verhasselt P., Riles L., Robben J., Volckaert G.
    Yeast 13:177-182(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Yeast ribosomal proteins. VIII. Isolation of two proteins and sequence characterization of twenty-four proteins from cytoplasmic ribosomes."
    Otaka E., Higo K., Itoh T.
    Mol. Gen. Genet. 195:544-546(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL PROTEIN SEQUENCE OF 1-40.
  6. "The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae."
    Planta R.J., Mager W.H.
    Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NOMENCLATURE, SUBUNIT.
  7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. "Structure of the 80S ribosome from Saccharomyces cerevisiae -- tRNA-ribosome and subunit-subunit interactions."
    Spahn C.M.T., Beckmann R., Eswar N., Penczek P.A., Sali A., Blobel G., Frank J.
    Cell 107:373-386(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: 3D-STRUCTURE MODELING OF 7-184, ELECTRON MICROSCOPY.
  10. "Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation."
    Spahn C.M.T., Gomez-Lorenzo M.G., Grassucci R.A., Joergensen R., Andersen G.R., Beckmann R., Penczek P.A., Ballesta J.P.G., Frank J.
    EMBO J. 23:1008-1019(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: 3D-STRUCTURE MODELING, ELECTRON MICROSCOPY.
  11. "Crystal structure of the eukaryotic ribosome."
    Ben-Shem A., Jenner L., Yusupova G., Yusupov M.
    Science 330:1203-1209(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (4.0 ANGSTROMS) OF 80S RIBOSOME.
  12. Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF 80S RIBOSOME.

Entry informationi

Entry nameiRL9A_YEAST
AccessioniPrimary (citable) accession number: P05738
Secondary accession number(s): D6VU02
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: December 1, 1992
Last modified: March 4, 2015
This is version 140 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 52400 molecules/cell in log phase SD medium.1 Publication
There are 2 genes for L9 in yeast.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.