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Protein

60S ribosomal protein L7-A

Gene

RPL7A

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

  1. structural constituent of ribosome Source: SGD

GO - Biological processi

  1. cytoplasmic translation Source: SGD
  2. maturation of LSU-rRNA Source: SGD
  3. ribosomal large subunit biogenesis Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Enzyme and pathway databases

BioCyciYEAST:G3O-30577-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
60S ribosomal protein L7-A
Alternative name(s):
L6
RP11
YL8
Gene namesi
Name:RPL7A
Synonyms:RPL6A, RPL8A, YL8A
Ordered Locus Names:YGL076C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome VII

Organism-specific databases

SGDiS000003044. RPL7A.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: SGD
  2. cytosolic large ribosomal subunit Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed3 Publications
Chaini2 – 24424360S ribosomal protein L7-APRO_0000104651Add
BLAST

Proteomic databases

MaxQBiP05737.
PeptideAtlasiP05737.

Expressioni

Gene expression databases

GenevestigatoriP05737.

Interactioni

Subunit structurei

Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S).1 Publication

Protein-protein interaction databases

BioGridi33174. 133 interactions.
IntActiP05737. 130 interactions.
MINTiMINT-4485679.
STRINGi4932.YGL076C.

Structurei

Secondary structure

1
244
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni23 – 253Combined sources
Helixi28 – 7144Combined sources
Beta strandi82 – 887Combined sources
Beta strandi92 – 943Combined sources
Helixi97 – 1059Combined sources
Beta strandi113 – 1186Combined sources
Helixi121 – 1288Combined sources
Turni129 – 1335Combined sources
Beta strandi134 – 1374Combined sources
Helixi141 – 15111Combined sources
Beta strandi156 – 1594Combined sources
Beta strandi160 – 1623Combined sources
Helixi166 – 1738Combined sources
Helixi174 – 1763Combined sources
Helixi181 – 1899Combined sources
Helixi195 – 2017Combined sources
Beta strandi215 – 2173Combined sources
Beta strandi219 – 2224Combined sources
Helixi223 – 2253Combined sources
Beta strandi227 – 2315Combined sources
Helixi233 – 2353Combined sources
Helixi236 – 2438Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1K5Ymodel-F83-243[»]
3J6Xelectron microscopy6.10L71-244[»]
3J6Yelectron microscopy6.10L71-244[»]
3J77electron microscopy6.20L71-244[»]
3J78electron microscopy6.30L71-244[»]
4U3MX-ray3.00L7/l72-244[»]
4U3NX-ray3.20L7/l72-244[»]
4U3UX-ray2.90L7/l72-244[»]
4U4NX-ray3.10L7/l72-244[»]
4U4OX-ray3.60L7/l72-244[»]
4U4QX-ray3.00L7/l72-244[»]
4U4RX-ray2.80L7/l72-244[»]
4U4UX-ray3.00L7/l72-244[»]
4U4YX-ray3.20L7/l72-244[»]
4U4ZX-ray3.10L7/l72-244[»]
4U50X-ray3.20L7/l72-244[»]
4U51X-ray3.20L7/l72-244[»]
4U52X-ray3.00L7/l72-244[»]
4U53X-ray3.30L7/l72-244[»]
4U55X-ray3.20L7/l72-244[»]
4U56X-ray3.45L7/l72-244[»]
4U6FX-ray3.10L7/l72-244[»]
4V4Belectron microscopy11.70BF83-244[»]
4V6Ielectron microscopy8.80Be1-244[»]
4V7Felectron microscopy8.70a1-244[»]
4V7RX-ray4.00BG/DG1-244[»]
4V88X-ray3.00BF/DF1-244[»]
4V8Telectron microscopy8.10F1-244[»]
4V8Yelectron microscopy4.30BF2-244[»]
4V8Zelectron microscopy6.60BF2-244[»]
4V91electron microscopy3.70F1-244[»]
SMRiP05737. Positions 22-244.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP05737.

Family & Domainsi

Sequence similaritiesi

Belongs to the ribosomal protein L30P family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000006213.
HOGENOMiHOG000170917.
InParanoidiP05737.
KOiK02937.
OMAiYRTETRM.
OrthoDBiEOG7TBCCW.

Family and domain databases

Gene3Di3.30.1390.20. 2 hits.
InterProiIPR018038. Ribosomal_L30_CS.
IPR016082. Ribosomal_L30_ferredoxin-like.
IPR012988. Ribosomal_L30_N.
IPR005998. Ribosomal_L7_euk.
[Graphical view]
PfamiPF00327. Ribosomal_L30. 1 hit.
PF08079. Ribosomal_L30_N. 1 hit.
[Graphical view]
SUPFAMiSSF55129. SSF55129. 1 hit.
TIGRFAMsiTIGR01310. L7. 1 hit.
PROSITEiPS00634. RIBOSOMAL_L30. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P05737-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAEKILTPE SQLKKSKAQQ KTAEQVAAER AARKAANKEK RAIILERNAA
60 70 80 90 100
YQKEYETAER NIIQAKRDAK AAGSYYVEAQ HKLVFVVRIK GINKIPPKPR
110 120 130 140 150
KVLQLLRLTR INSGTFVKVT KATLELLKLI EPYVAYGYPS YSTIRQLVYK
160 170 180 190 200
RGFGKINKQR VPLSDNAIIE ANLGKYGILS IDDLIHEIIT VGPHFKQANN
210 220 230 240
FLWPFKLSNP SGGWGVPRKF KHFIQGGSFG NREEFINKLV KSMN
Length:244
Mass (Da):27,638
Last modified:January 23, 2007 - v3
Checksum:iF65B24D48459F745
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X62627 Genomic DNA. Translation: CAA44495.1.
Z72598 Genomic DNA. Translation: CAA96781.1.
Z72597 Genomic DNA. Translation: CAA96779.2. Sequence problems.
BK006941 Genomic DNA. Translation: DAA08029.1.
PIRiS22789. R5BYL7.
RefSeqiNP_011439.1. NM_001180941.1.

Genome annotation databases

EnsemblFungiiYGL076C; YGL076C; YGL076C.
GeneIDi852804.
KEGGisce:YGL076C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X62627 Genomic DNA. Translation: CAA44495.1.
Z72598 Genomic DNA. Translation: CAA96781.1.
Z72597 Genomic DNA. Translation: CAA96779.2. Sequence problems.
BK006941 Genomic DNA. Translation: DAA08029.1.
PIRiS22789. R5BYL7.
RefSeqiNP_011439.1. NM_001180941.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1K5Ymodel-F83-243[»]
3J6Xelectron microscopy6.10L71-244[»]
3J6Yelectron microscopy6.10L71-244[»]
3J77electron microscopy6.20L71-244[»]
3J78electron microscopy6.30L71-244[»]
4U3MX-ray3.00L7/l72-244[»]
4U3NX-ray3.20L7/l72-244[»]
4U3UX-ray2.90L7/l72-244[»]
4U4NX-ray3.10L7/l72-244[»]
4U4OX-ray3.60L7/l72-244[»]
4U4QX-ray3.00L7/l72-244[»]
4U4RX-ray2.80L7/l72-244[»]
4U4UX-ray3.00L7/l72-244[»]
4U4YX-ray3.20L7/l72-244[»]
4U4ZX-ray3.10L7/l72-244[»]
4U50X-ray3.20L7/l72-244[»]
4U51X-ray3.20L7/l72-244[»]
4U52X-ray3.00L7/l72-244[»]
4U53X-ray3.30L7/l72-244[»]
4U55X-ray3.20L7/l72-244[»]
4U56X-ray3.45L7/l72-244[»]
4U6FX-ray3.10L7/l72-244[»]
4V4Belectron microscopy11.70BF83-244[»]
4V6Ielectron microscopy8.80Be1-244[»]
4V7Felectron microscopy8.70a1-244[»]
4V7RX-ray4.00BG/DG1-244[»]
4V88X-ray3.00BF/DF1-244[»]
4V8Telectron microscopy8.10F1-244[»]
4V8Yelectron microscopy4.30BF2-244[»]
4V8Zelectron microscopy6.60BF2-244[»]
4V91electron microscopy3.70F1-244[»]
SMRiP05737. Positions 22-244.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33174. 133 interactions.
IntActiP05737. 130 interactions.
MINTiMINT-4485679.
STRINGi4932.YGL076C.

Proteomic databases

MaxQBiP05737.
PeptideAtlasiP05737.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGL076C; YGL076C; YGL076C.
GeneIDi852804.
KEGGisce:YGL076C.

Organism-specific databases

SGDiS000003044. RPL7A.

Phylogenomic databases

GeneTreeiENSGT00390000006213.
HOGENOMiHOG000170917.
InParanoidiP05737.
KOiK02937.
OMAiYRTETRM.
OrthoDBiEOG7TBCCW.

Enzyme and pathway databases

BioCyciYEAST:G3O-30577-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP05737.
NextBioi972327.

Gene expression databases

GenevestigatoriP05737.

Family and domain databases

Gene3Di3.30.1390.20. 2 hits.
InterProiIPR018038. Ribosomal_L30_CS.
IPR016082. Ribosomal_L30_ferredoxin-like.
IPR012988. Ribosomal_L30_N.
IPR005998. Ribosomal_L7_euk.
[Graphical view]
PfamiPF00327. Ribosomal_L30. 1 hit.
PF08079. Ribosomal_L30_N. 1 hit.
[Graphical view]
SUPFAMiSSF55129. SSF55129. 1 hit.
TIGRFAMsiTIGR01310. L7. 1 hit.
PROSITEiPS00634. RIBOSOMAL_L30. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Yeast ribosomal proteins: XIII. Saccharomyces cerevisiae YL8A gene, interrupted with two introns, encodes a homolog of mammalian L7."
    Mizuta K., Hashimoto T., Otaka E.
    Nucleic Acids Res. 20:1011-1016(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Sequence analysis of 203 kilobases from Saccharomyces cerevisiae chromosome VII."
    Rieger M., Brueckner M., Schaefer M., Mueller-Auer S.
    Yeast 13:1077-1090(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Yeast ribosomal proteins. VIII. Isolation of two proteins and sequence characterization of twenty-four proteins from cytoplasmic ribosomes."
    Otaka E., Higo K., Itoh T.
    Mol. Gen. Genet. 195:544-546(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL PROTEIN SEQUENCE OF 2-41, CLEAVAGE OF INITIATOR METHIONINE.
  6. "NH2-terminal acetylation of ribosomal proteins of Saccharomyces cerevisiae."
    Takakura H., Tsunasawa S., Miyagi M., Warner J.R.
    J. Biol. Chem. 267:5442-5445(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-21.
  7. "The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae."
    Planta R.J., Mager W.H.
    Yeast 14:471-477(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NOMENCLATURE, SUBUNIT.
  8. "The action of N-terminal acetyltransferases on yeast ribosomal proteins."
    Arnold R.J., Polevoda B., Reilly J.P., Sherman F.
    J. Biol. Chem. 274:37035-37040(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: CLEAVAGE OF INITIATOR METHIONINE.
  9. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  10. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  11. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. "Structure of the 80S ribosome from Saccharomyces cerevisiae -- tRNA-ribosome and subunit-subunit interactions."
    Spahn C.M.T., Beckmann R., Eswar N., Penczek P.A., Sali A., Blobel G., Frank J.
    Cell 107:373-386(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: 3D-STRUCTURE MODELING OF 83-243, ELECTRON MICROSCOPY.
  13. "Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation."
    Spahn C.M.T., Gomez-Lorenzo M.G., Grassucci R.A., Joergensen R., Andersen G.R., Beckmann R., Penczek P.A., Ballesta J.P.G., Frank J.
    EMBO J. 23:1008-1019(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: 3D-STRUCTURE MODELING OF 83-244, ELECTRON MICROSCOPY.
  14. "Crystal structure of the eukaryotic ribosome."
    Ben-Shem A., Jenner L., Yusupova G., Yusupov M.
    Science 330:1203-1209(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (4.0 ANGSTROMS) OF 80S RIBOSOME.
  15. Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF 80S RIBOSOME.

Entry informationi

Entry nameiRL7A_YEAST
AccessioniPrimary (citable) accession number: P05737
Secondary accession number(s): D6VU68, P87029
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: January 23, 2007
Last modified: April 1, 2015
This is version 151 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 101000 molecules/cell in log phase SD medium.1 Publication
There are 2 genes for L7 in yeast.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.