P05737 (RL7A_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
60S ribosomal protein L7-A

Alternative name(s):
L6
RP11
YL8

Gene names

Name:	RPL7A
Synonyms:	RPL6A, RPL8A, YL8A
Ordered Locus Names:	YGL076C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	244 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). Ref.7
Subcellular location	Cytoplasm Ref.10.
Miscellaneous	Present with 101000 molecules/cell in log phase SD medium. Ref.11 There are 2 genes for L7 in yeast.
Sequence similarities	Belongs to the ribosomal protein L30P family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Ribonucleoprotein Ribosomal protein
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	cytoplasmic translation Inferred from direct assay. Source: SGD
Cellular component	cytosolic large ribosomal subunit Inferred from direct assay. Source: SGD
Molecular function	structural constituent of ribosome Inferred from direct assay. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
YML131W	Q03102	1	EBI-15422,EBI-27999

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.5 Ref.6 Ref.8
	Chain	2 – 244	243	60S ribosomal protein L7-A		PRO_0000104651
Amino acid modifications
	Modified residue	8	1	Phosphothreonine Ref.9 Ref.12 Ref.13 Ref.14 Ref.15 Ref.16
	Modified residue	11	1	Phosphoserine Ref.9 Ref.13 Ref.14 Ref.16

Sequences

	Sequence		Length	Mass (Da)
	P05737 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: F65B24D48459F745	FASTA	244	27,638
	10 20 30 40 50 60 MAAEKILTPE SQLKKSKAQQ KTAEQVAAER AARKAANKEK RAIILERNAA YQKEYETAER 70 80 90 100 110 120 NIIQAKRDAK AAGSYYVEAQ HKLVFVVRIK GINKIPPKPR KVLQLLRLTR INSGTFVKVT 130 140 150 160 170 180 KATLELLKLI EPYVAYGYPS YSTIRQLVYK RGFGKINKQR VPLSDNAIIE ANLGKYGILS 190 200 210 220 230 240 IDDLIHEIIT VGPHFKQANN FLWPFKLSNP SGGWGVPRKF KHFIQGGSFG NREEFINKLV KSMN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Yeast ribosomal proteins: XIII. Saccharomyces cerevisiae YL8A gene, interrupted with two introns, encodes a homolog of mammalian L7." Mizuta K., Hashimoto T., Otaka E. Nucleic Acids Res. 20:1011-1016(1992) [PubMed: 1549461] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequence analysis of 203 kilobases from Saccharomyces cerevisiae chromosome VII." Rieger M., Brueckner M., Schaefer M., Mueller-Auer S. Yeast 13:1077-1090(1997) [PubMed: 9290212] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Yeast ribosomal proteins. VIII. Isolation of two proteins and sequence characterization of twenty-four proteins from cytoplasmic ribosomes." Otaka E., Higo K., Itoh T. Mol. Gen. Genet. 195:544-546(1984) Cited for: PROTEIN SEQUENCE OF 2-41.
[6]	"NH2-terminal acetylation of ribosomal proteins of Saccharomyces cerevisiae." Takakura H., Tsunasawa S., Miyagi M., Warner J.R. J. Biol. Chem. 267:5442-5445(1992) [PubMed: 1544921] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-21.
[7]	"The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae." Planta R.J., Mager W.H. Yeast 14:471-477(1998) [PubMed: 9559554] [Abstract] Cited for: NOMENCLATURE, SUBUNIT.
[8]	"The action of N-terminal acetyltransferases on yeast ribosomal proteins." Arnold R.J., Polevoda B., Reilly J.P., Sherman F. J. Biol. Chem. 274:37035-37040(1999) [PubMed: 10601260] [Abstract] Cited for: CLEAVAGE OF INITIATOR METHIONINE.
[9]	"Phosphoproteome analysis by mass spectrometry and its application to Saccharomyces cerevisiae." Ficarro S.B., McCleland M.L., Stukenberg P.T., Burke D.J., Ross M.M., Shabanowitz J., Hunt D.F., White F.M. Nat. Biotechnol. 20:301-305(2002) [PubMed: 11875433] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-8 AND SER-11, MASS SPECTROMETRY. Strain: 2124.
[10]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[11]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[12]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed: 15665377] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-8, MASS SPECTROMETRY. Strain: YAL6B.
[13]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-8 AND SER-11, MASS SPECTROMETRY. Strain: ADR376.
[14]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-8 AND SER-11, MASS SPECTROMETRY.
[15]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-8, MASS SPECTROMETRY.
[16]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-8 AND SER-11, MASS SPECTROMETRY.
[17]	"Structure of the 80S ribosome from Saccharomyces cerevisiae -- tRNA-ribosome and subunit-subunit interactions." Spahn C.M.T., Beckmann R., Eswar N., Penczek P.A., Sali A., Blobel G., Frank J. Cell 107:373-386(2001) [PubMed: 11701127] [Abstract] Cited for: 3D-STRUCTURE MODELING OF 83-243, ELECTRON MICROSCOPY.
[18]	"Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation." Spahn C.M.T., Gomez-Lorenzo M.G., Grassucci R.A., Joergensen R., Andersen G.R., Beckmann R., Penczek P.A., Ballesta J.P.G., Frank J. EMBO J. 23:1008-1019(2004) [PubMed: 14976550] [Abstract] Cited for: 3D-STRUCTURE MODELING OF 83-244, ELECTRON MICROSCOPY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X62627 Genomic DNA. Translation: CAA44495.1.
Z72598 Genomic DNA. Translation: CAA96781.1.
Z72597 Genomic DNA. Translation: CAA96779.2. Sequence problems.
BK006941 Genomic DNA. Translation: DAA08029.1.

PIR

R5BYL7. S22789.

RefSeq

NP_011439.1. NM_001180941.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1K5Y	model	-	F	83-243	[»]
1S1I	electron microscopy	11.70	F	83-244	[»]
3IZS	electron microscopy	-	e	1-244	[»]
3O58	X-ray	4.00	G	1-244	[»]
3O5H	X-ray	4.00	G	1-244	[»]

ProteinModelPortal

P05737.

SMR

P05737. Positions 22-244.

ModBase

Search...

Protein-protein interaction databases

IntAct

P05737. 127 interactions.

STRING

P05737.

Proteomic databases

PeptideAtlas

P05737.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YGL076C; YGL076C; YGL076C.

GeneID

852804.

KEGG

sce:YGL076C.

NMPDR

fig|4932.3.peg.2544.

Organism-specific databases

SGD

S000003044. RPL7A.

Phylogenomic databases

eggNOG

fuNOG04188.

GeneTree

EFGT00050000004263.

HOGENOM

HBG618766.

OMA

RAKQYSK.

OrthoDB

EOG4BZRBN.

Gene expression databases

ArrayExpress

P05737.

Genevestigator

P05737.

GermOnline

YGL076C. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR018038. Ribosomal_L30_CS.
IPR016082. Ribosomal_L30_ferredoxin-like.
IPR012988. Ribosomal_L30_N.
IPR005998. Ribosomal_L7_euk.
[Graphical view]

Gene3D

G3DSA:3.30.1390.20. Ribosomal_L30_ferredoxin-like. 2 hits.

KO

K02937.

Pfam

PF00327. Ribosomal_L30. 1 hit.
PF08079. Ribosomal_L30_N. 1 hit.
[Graphical view]

SUPFAM

SSF55129. Ribosomal_L30. 1 hit.

TIGRFAMs

TIGR01310. L7. 1 hit.

PROSITE

PS00634. RIBOSOMAL_L30. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

972327.

Entry information

Entry name

RL7A_YEAST

Accession

Primary (citable) accession number: P05737
Secondary accession number(s): D6VU68, P87029

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1988
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 122 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program