Type-1 restriction enzyme EcoKI specificity protein

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P05719 (T1SK_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 108. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Type-1 restriction enzyme EcoKI specificity protein
Short name=S.EcoKI

Alternative name(s):
Type I restriction enzyme EcoKI specificity protein
Short name=S protein

Gene names

Name:	hsdS
Synonyms:	hss
Ordered Locus Names:	b4348, JW4311

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length	464 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to the same target sequence but cutting the DNA some distance from this site. Whether the DNA is cut or modified depends on the methylation state of the target sequence. When the target site is unmodified, the DNA is cut. When the target site is hemimethylated, the complex acts as a maintenance MTase modifying the DNA so that both strands become methylated. Subunit S dictates DNA sequences specificity. The EcoKI enzyme recognizes 5'-AACN₆GTGC-3'.
Subunit structure	The type I restriction/modification system is composed of three polypeptides R, M and S.
Domain	Contains two DNA recognition domains, each specifying recognition of one of the two defined components of the target sequence.
Sequence similarities	Belongs to the type-I restriction system S methylase family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	DNA-binding
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ftsH	P0AAI3	1	EBI-1135164,EBI-548381

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 464

464

Type-1 restriction enzyme EcoKI specificity protein

PRO_0000198035

Sequences

Sequence

Length

Mass (Da)

Tools

P05719 [UniParc].

Last modified November 1, 1988. Version 1.
Checksum: 1D651D0716B08C04
Show »

FASTA

464

51,395

        10         20         30         40         50         60 
MSAGKLPEGW VIAPVSTVTT LIRGVTYKKE QAINYLKDDY LPLIRANNIQ NGKFDTTDLV 

        70         80         90        100        110        120 
FVPKNLVKES QKISPEDIVI AMSSGSKSVV GKSAHQHLPF ECSFGAFCGV LRPEKLIFSG 

       130        140        150        160        170        180 
FIAHFTKSSL YRNKISSLSA GANINNIKPA SFDLINIPIP PLAEQKIIAE KLDTLLAQVD 

       190        200        210        220        230        240 
STKARFEQIP QILKRFRQAV LGGAVNGKLT EKWRNFEPQH SVFKKLNFES ILTELRNGLS 

       250        260        270        280        290        300 
SKPNESGVGH PILRISSVRA GHVDQNDIRF LECSESELNR HKLQDGDLLF TRYNGSLEFV 

       310        320        330        340        350        360 
GVCGLLKKLQ HQNLLYPDKL IRARLTKDAL PEYIEIFFSS PSARNAMMNC VKTTSGQKGI 

       370        380        390        400        410        420 
SGKDIKSQVV LLPPVKEQAE IVRRVEQLFA YADTIEKQVN NALARVNNLT QSILAKAFRG 

       430        440        450        460 
ELTAQWRAEN PDLISGENSA AALLEKIKAE RAASGGKKAS RKKS

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence diversity among related genes for recognition of specific targets in DNA molecules." Gough J.A., Murray N.E. J. Mol. Biol. 166:1-19(1983) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R. Nucleic Acids Res. 23:2105-2119(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Tyrosine 27 of the specificity polypeptide of EcoKI can be UV crosslinked to a bromodeoxyuridine-substituted DNA target sequence." Chen A., Powell L.M., Dryden D.T., Murray N.E., Brown T. Nucleic Acids Res. 23:1177-1183(1995) [PubMed] [Europe PMC] [Abstract] Cited for: PARTIAL PROTEIN SEQUENCE.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

V00288 Genomic DNA. Translation: CAA23554.1.
U14003 Genomic DNA. Translation: AAA97245.1.
U00096 Genomic DNA. Translation: AAC77304.1.
AP009048 Genomic DNA. Translation: BAE78338.1.

PIR

NDECKS. A30369.

RefSeq

NP_418768.1. NC_000913.2.
YP_492479.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2Y7C	electron microscopy	18.00	A	1-464	[»]
2Y7H	electron microscopy	18.00	A	1-464	[»]

ProteinModelPortal

P05719.

SMR

P05719. Positions 1-464.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-9945N.

IntAct

P05719. 1 interaction.

MINT

MINT-1225773.

STRING

511145.b4348.

Protein family/group databases

REBASE

3646. S.EcoKI.

Proteomic databases

PaxDb

P05719.

PRIDE

P05719.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAC77304; AAC77304; b4348.
BAE78338; BAE78338; BAE78338.

GeneID

12933773.
948867.

KEGG

ecj:Y75_p4233.
eco:b4348.

PATRIC

32124302. VBIEscCol129921_4494.

Organism-specific databases

EchoBASE

EB0455.

EcoGene

EG10460. hsdS.

Phylogenomic databases

eggNOG

COG0732.

HOGENOM

HOG000295029.

K01154.

OMA

MLAIDAC.

ProtClustDB

PRK09737.

Enzyme and pathway databases

BioCyc

EcoCyc:EG10460-MONOMER.
ECOL316407:JW4311-MONOMER.
MetaCyc:EG10460-MONOMER.

Gene expression databases

Genevestigator

P05719.

Family and domain databases

InterPro

IPR000055. Restrct_endonuc_typeI_HsdS.
[Graphical view]

Pfam

PF01420. Methylase_S. 2 hits.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P05719.

Entry information

Entry name

T1SK_ECOLI

Accession

Primary (citable) accession number: P05719
Secondary accession number(s): Q2M5W8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1988
Last sequence update:	November 1, 1988
Last modified:	May 1, 2013
This is version 108 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents