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Protein

Type-1 restriction enzyme EcoKI specificity protein

Gene

hsdS

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to the same target sequence but cutting the DNA some distance from this site. Whether the DNA is cut or modified depends on the methylation state of the target sequence. When the target site is unmodified, the DNA is cut. When the target site is hemimethylated, the complex acts as a maintenance MTase modifying the DNA so that both strands become methylated. Subunit S dictates DNA sequences specificity. The EcoKI enzyme recognizes 5'-AACN6GTGC-3'.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding
Biological processRestriction system

Enzyme and pathway databases

BioCyciEcoCyc:EG10460-MONOMER
MetaCyc:EG10460-MONOMER

Protein family/group databases

REBASEi3646 S.EcoKI

Names & Taxonomyi

Protein namesi
Recommended name:
Type-1 restriction enzyme EcoKI specificity protein
Short name:
S.EcoKI
Alternative name(s):
Type I restriction enzyme EcoKI specificity protein
Short name:
S protein
Gene namesi
Name:hsdS
Synonyms:hss
Ordered Locus Names:b4348, JW4311
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10460 hsdS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001980351 – 464Type-1 restriction enzyme EcoKI specificity proteinAdd BLAST464

Proteomic databases

PaxDbiP05719
PRIDEiP05719

Interactioni

Subunit structurei

The type I restriction/modification system is composed of three polypeptides R, M and S.

Protein-protein interaction databases

BioGridi4262767, 128 interactors
DIPiDIP-9945N
IntActiP05719, 12 interactors
STRINGi316407.85677088

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2Y7Celectron microscopy18.00A1-464[»]
2Y7Helectron microscopy18.00A1-464[»]
ProteinModelPortaliP05719
SMRiP05719
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP05719

Family & Domainsi

Domaini

Contains two DNA recognition domains, each specifying recognition of one of the two defined components of the target sequence.

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4107XZC Bacteria
COG0732 LUCA
HOGENOMiHOG000295029
InParanoidiP05719
KOiK01154
OMAiQQTVEYL
PhylomeDBiP05719

Family and domain databases

InterProiView protein in InterPro
IPR000055 Restrct_endonuc_typeI_HsdS
PfamiView protein in Pfam
PF01420 Methylase_S, 2 hits

Sequencei

Sequence statusi: Complete.

P05719-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAGKLPEGW VIAPVSTVTT LIRGVTYKKE QAINYLKDDY LPLIRANNIQ
60 70 80 90 100
NGKFDTTDLV FVPKNLVKES QKISPEDIVI AMSSGSKSVV GKSAHQHLPF
110 120 130 140 150
ECSFGAFCGV LRPEKLIFSG FIAHFTKSSL YRNKISSLSA GANINNIKPA
160 170 180 190 200
SFDLINIPIP PLAEQKIIAE KLDTLLAQVD STKARFEQIP QILKRFRQAV
210 220 230 240 250
LGGAVNGKLT EKWRNFEPQH SVFKKLNFES ILTELRNGLS SKPNESGVGH
260 270 280 290 300
PILRISSVRA GHVDQNDIRF LECSESELNR HKLQDGDLLF TRYNGSLEFV
310 320 330 340 350
GVCGLLKKLQ HQNLLYPDKL IRARLTKDAL PEYIEIFFSS PSARNAMMNC
360 370 380 390 400
VKTTSGQKGI SGKDIKSQVV LLPPVKEQAE IVRRVEQLFA YADTIEKQVN
410 420 430 440 450
NALARVNNLT QSILAKAFRG ELTAQWRAEN PDLISGENSA AALLEKIKAE
460
RAASGGKKAS RKKS
Length:464
Mass (Da):51,395
Last modified:November 1, 1988 - v1
Checksum:i1D651D0716B08C04
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00288 Genomic DNA Translation: CAA23554.1
U14003 Genomic DNA Translation: AAA97245.1
U00096 Genomic DNA Translation: AAC77304.1
AP009048 Genomic DNA Translation: BAE78338.1
PIRiA30369 NDECKS
RefSeqiNP_418768.1, NC_000913.3
WP_001272447.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC77304; AAC77304; b4348
BAE78338; BAE78338; BAE78338
GeneIDi948867
KEGGiecj:JW4311
eco:b4348
PATRICifig|1411691.4.peg.2338

Similar proteinsi

Entry informationi

Entry nameiT1SK_ECOLI
AccessioniPrimary (citable) accession number: P05719
Secondary accession number(s): Q2M5W8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: November 1, 1988
Last modified: March 28, 2018
This is version 134 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health