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Protein

Type-1 restriction enzyme EcoKI specificity protein

Gene

hsdS

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to the same target sequence but cutting the DNA some distance from this site. Whether the DNA is cut or modified depends on the methylation state of the target sequence. When the target site is unmodified, the DNA is cut. When the target site is hemimethylated, the complex acts as a maintenance MTase modifying the DNA so that both strands become methylated. Subunit S dictates DNA sequences specificity. The EcoKI enzyme recognizes 5'-AACN6GTGC-3'.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Restriction system

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG10460-MONOMER.
ECOL316407:JW4311-MONOMER.
MetaCyc:EG10460-MONOMER.

Protein family/group databases

REBASEi3646. S.EcoKI.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-1 restriction enzyme EcoKI specificity protein
Short name:
S.EcoKI
Alternative name(s):
Type I restriction enzyme EcoKI specificity protein
Short name:
S protein
Gene namesi
Name:hsdS
Synonyms:hss
Ordered Locus Names:b4348, JW4311
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318 Componenti: Chromosome UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10460. hsdS.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 464464Type-1 restriction enzyme EcoKI specificity proteinPRO_0000198035Add
BLAST

Proteomic databases

PaxDbiP05719.
PRIDEiP05719.

Interactioni

Subunit structurei

The type I restriction/modification system is composed of three polypeptides R, M and S.

Binary interactionsi

WithEntry#Exp.IntActNotes
ftsHP0AAI31EBI-1135164,EBI-548381

Protein-protein interaction databases

DIPiDIP-9945N.
IntActiP05719. 1 interaction.
MINTiMINT-1225773.
STRINGi511145.b4348.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2Y7Celectron microscopy18.00A1-464[»]
2Y7Helectron microscopy18.00A1-464[»]
ProteinModelPortaliP05719.
SMRiP05719. Positions 1-464.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP05719.

Family & Domainsi

Domaini

Contains two DNA recognition domains, each specifying recognition of one of the two defined components of the target sequence.

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0732.
HOGENOMiHOG000295029.
InParanoidiP05719.
KOiK01154.
OMAiMLAIDAC.
OrthoDBiEOG62ZHW5.
PhylomeDBiP05719.

Family and domain databases

InterProiIPR000055. Restrct_endonuc_typeI_HsdS.
[Graphical view]
PfamiPF01420. Methylase_S. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P05719-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAGKLPEGW VIAPVSTVTT LIRGVTYKKE QAINYLKDDY LPLIRANNIQ
60 70 80 90 100
NGKFDTTDLV FVPKNLVKES QKISPEDIVI AMSSGSKSVV GKSAHQHLPF
110 120 130 140 150
ECSFGAFCGV LRPEKLIFSG FIAHFTKSSL YRNKISSLSA GANINNIKPA
160 170 180 190 200
SFDLINIPIP PLAEQKIIAE KLDTLLAQVD STKARFEQIP QILKRFRQAV
210 220 230 240 250
LGGAVNGKLT EKWRNFEPQH SVFKKLNFES ILTELRNGLS SKPNESGVGH
260 270 280 290 300
PILRISSVRA GHVDQNDIRF LECSESELNR HKLQDGDLLF TRYNGSLEFV
310 320 330 340 350
GVCGLLKKLQ HQNLLYPDKL IRARLTKDAL PEYIEIFFSS PSARNAMMNC
360 370 380 390 400
VKTTSGQKGI SGKDIKSQVV LLPPVKEQAE IVRRVEQLFA YADTIEKQVN
410 420 430 440 450
NALARVNNLT QSILAKAFRG ELTAQWRAEN PDLISGENSA AALLEKIKAE
460
RAASGGKKAS RKKS
Length:464
Mass (Da):51,395
Last modified:November 1, 1988 - v1
Checksum:i1D651D0716B08C04
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00288 Genomic DNA. Translation: CAA23554.1.
U14003 Genomic DNA. Translation: AAA97245.1.
U00096 Genomic DNA. Translation: AAC77304.1.
AP009048 Genomic DNA. Translation: BAE78338.1.
PIRiA30369. NDECKS.
RefSeqiNP_418768.1. NC_000913.3.
WP_001272447.1. NZ_CP010445.1.

Genome annotation databases

EnsemblBacteriaiAAC77304; AAC77304; b4348.
BAE78338; BAE78338; BAE78338.
GeneIDi948867.
KEGGieco:b4348.
PATRICi32124302. VBIEscCol129921_4494.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00288 Genomic DNA. Translation: CAA23554.1.
U14003 Genomic DNA. Translation: AAA97245.1.
U00096 Genomic DNA. Translation: AAC77304.1.
AP009048 Genomic DNA. Translation: BAE78338.1.
PIRiA30369. NDECKS.
RefSeqiNP_418768.1. NC_000913.3.
WP_001272447.1. NZ_CP010445.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2Y7Celectron microscopy18.00A1-464[»]
2Y7Helectron microscopy18.00A1-464[»]
ProteinModelPortaliP05719.
SMRiP05719. Positions 1-464.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-9945N.
IntActiP05719. 1 interaction.
MINTiMINT-1225773.
STRINGi511145.b4348.

Protein family/group databases

REBASEi3646. S.EcoKI.

Proteomic databases

PaxDbiP05719.
PRIDEiP05719.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77304; AAC77304; b4348.
BAE78338; BAE78338; BAE78338.
GeneIDi948867.
KEGGieco:b4348.
PATRICi32124302. VBIEscCol129921_4494.

Organism-specific databases

EchoBASEiEB0455.
EcoGeneiEG10460. hsdS.

Phylogenomic databases

eggNOGiCOG0732.
HOGENOMiHOG000295029.
InParanoidiP05719.
KOiK01154.
OMAiMLAIDAC.
OrthoDBiEOG62ZHW5.
PhylomeDBiP05719.

Enzyme and pathway databases

BioCyciEcoCyc:EG10460-MONOMER.
ECOL316407:JW4311-MONOMER.
MetaCyc:EG10460-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP05719.
PROiP05719.

Family and domain databases

InterProiIPR000055. Restrct_endonuc_typeI_HsdS.
[Graphical view]
PfamiPF01420. Methylase_S. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence diversity among related genes for recognition of specific targets in DNA molecules."
    Gough J.A., Murray N.E.
    J. Mol. Biol. 166:1-19(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes."
    Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.
    Nucleic Acids Res. 23:2105-2119(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Tyrosine 27 of the specificity polypeptide of EcoKI can be UV crosslinked to a bromodeoxyuridine-substituted DNA target sequence."
    Chen A., Powell L.M., Dryden D.T., Murray N.E., Brown T.
    Nucleic Acids Res. 23:1177-1183(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL PROTEIN SEQUENCE.

Entry informationi

Entry nameiT1SK_ECOLI
AccessioniPrimary (citable) accession number: P05719
Secondary accession number(s): Q2M5W8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: November 1, 1988
Last modified: July 22, 2015
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.