Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P05719

- T1SK_ECOLI

UniProt

P05719 - T1SK_ECOLI

Protein

Type-1 restriction enzyme EcoKI specificity protein

Gene

hsdS

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 1 (01 Nov 1988)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to the same target sequence but cutting the DNA some distance from this site. Whether the DNA is cut or modified depends on the methylation state of the target sequence. When the target site is unmodified, the DNA is cut. When the target site is hemimethylated, the complex acts as a maintenance MTase modifying the DNA so that both strands become methylated. Subunit S dictates DNA sequences specificity. The EcoKI enzyme recognizes 5'-AACN6GTGC-3'.

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Biological processi

    Restriction system

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:EG10460-MONOMER.
    ECOL316407:JW4311-MONOMER.
    MetaCyc:EG10460-MONOMER.

    Protein family/group databases

    REBASEi3646. S.EcoKI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Type-1 restriction enzyme EcoKI specificity protein
    Short name:
    S.EcoKI
    Alternative name(s):
    Type I restriction enzyme EcoKI specificity protein
    Short name:
    S protein
    Gene namesi
    Name:hsdS
    Synonyms:hss
    Ordered Locus Names:b4348, JW4311
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10460. hsdS.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 464464Type-1 restriction enzyme EcoKI specificity proteinPRO_0000198035Add
    BLAST

    Proteomic databases

    PaxDbiP05719.
    PRIDEiP05719.

    Expressioni

    Gene expression databases

    GenevestigatoriP05719.

    Interactioni

    Subunit structurei

    The type I restriction/modification system is composed of three polypeptides R, M and S.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    ftsHP0AAI31EBI-1135164,EBI-548381

    Protein-protein interaction databases

    DIPiDIP-9945N.
    IntActiP05719. 1 interaction.
    MINTiMINT-1225773.
    STRINGi511145.b4348.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2Y7Celectron microscopy18.00A1-464[»]
    2Y7Helectron microscopy18.00A1-464[»]
    ProteinModelPortaliP05719.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP05719.

    Family & Domainsi

    Domaini

    Contains two DNA recognition domains, each specifying recognition of one of the two defined components of the target sequence.

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0732.
    HOGENOMiHOG000295029.
    KOiK01154.
    OMAiMLAIDAC.
    OrthoDBiEOG62ZHW5.
    PhylomeDBiP05719.

    Family and domain databases

    InterProiIPR000055. Restrct_endonuc_typeI_HsdS.
    [Graphical view]
    PfamiPF01420. Methylase_S. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P05719-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSAGKLPEGW VIAPVSTVTT LIRGVTYKKE QAINYLKDDY LPLIRANNIQ    50
    NGKFDTTDLV FVPKNLVKES QKISPEDIVI AMSSGSKSVV GKSAHQHLPF 100
    ECSFGAFCGV LRPEKLIFSG FIAHFTKSSL YRNKISSLSA GANINNIKPA 150
    SFDLINIPIP PLAEQKIIAE KLDTLLAQVD STKARFEQIP QILKRFRQAV 200
    LGGAVNGKLT EKWRNFEPQH SVFKKLNFES ILTELRNGLS SKPNESGVGH 250
    PILRISSVRA GHVDQNDIRF LECSESELNR HKLQDGDLLF TRYNGSLEFV 300
    GVCGLLKKLQ HQNLLYPDKL IRARLTKDAL PEYIEIFFSS PSARNAMMNC 350
    VKTTSGQKGI SGKDIKSQVV LLPPVKEQAE IVRRVEQLFA YADTIEKQVN 400
    NALARVNNLT QSILAKAFRG ELTAQWRAEN PDLISGENSA AALLEKIKAE 450
    RAASGGKKAS RKKS 464
    Length:464
    Mass (Da):51,395
    Last modified:November 1, 1988 - v1
    Checksum:i1D651D0716B08C04
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    V00288 Genomic DNA. Translation: CAA23554.1.
    U14003 Genomic DNA. Translation: AAA97245.1.
    U00096 Genomic DNA. Translation: AAC77304.1.
    AP009048 Genomic DNA. Translation: BAE78338.1.
    PIRiA30369. NDECKS.
    RefSeqiNP_418768.1. NC_000913.3.
    YP_492479.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC77304; AAC77304; b4348.
    BAE78338; BAE78338; BAE78338.
    GeneIDi12933773.
    948867.
    KEGGiecj:Y75_p4233.
    eco:b4348.
    PATRICi32124302. VBIEscCol129921_4494.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    V00288 Genomic DNA. Translation: CAA23554.1 .
    U14003 Genomic DNA. Translation: AAA97245.1 .
    U00096 Genomic DNA. Translation: AAC77304.1 .
    AP009048 Genomic DNA. Translation: BAE78338.1 .
    PIRi A30369. NDECKS.
    RefSeqi NP_418768.1. NC_000913.3.
    YP_492479.1. NC_007779.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2Y7C electron microscopy 18.00 A 1-464 [» ]
    2Y7H electron microscopy 18.00 A 1-464 [» ]
    ProteinModelPortali P05719.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-9945N.
    IntActi P05719. 1 interaction.
    MINTi MINT-1225773.
    STRINGi 511145.b4348.

    Protein family/group databases

    REBASEi 3646. S.EcoKI.

    Proteomic databases

    PaxDbi P05719.
    PRIDEi P05719.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC77304 ; AAC77304 ; b4348 .
    BAE78338 ; BAE78338 ; BAE78338 .
    GeneIDi 12933773.
    948867.
    KEGGi ecj:Y75_p4233.
    eco:b4348.
    PATRICi 32124302. VBIEscCol129921_4494.

    Organism-specific databases

    EchoBASEi EB0455.
    EcoGenei EG10460. hsdS.

    Phylogenomic databases

    eggNOGi COG0732.
    HOGENOMi HOG000295029.
    KOi K01154.
    OMAi MLAIDAC.
    OrthoDBi EOG62ZHW5.
    PhylomeDBi P05719.

    Enzyme and pathway databases

    BioCyci EcoCyc:EG10460-MONOMER.
    ECOL316407:JW4311-MONOMER.
    MetaCyc:EG10460-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P05719.
    PROi P05719.

    Gene expression databases

    Genevestigatori P05719.

    Family and domain databases

    InterProi IPR000055. Restrct_endonuc_typeI_HsdS.
    [Graphical view ]
    Pfami PF01420. Methylase_S. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence diversity among related genes for recognition of specific targets in DNA molecules."
      Gough J.A., Murray N.E.
      J. Mol. Biol. 166:1-19(1983) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12.
    2. "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes."
      Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.
      Nucleic Acids Res. 23:2105-2119(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    5. "Tyrosine 27 of the specificity polypeptide of EcoKI can be UV crosslinked to a bromodeoxyuridine-substituted DNA target sequence."
      Chen A., Powell L.M., Dryden D.T., Murray N.E., Brown T.
      Nucleic Acids Res. 23:1177-1183(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: PARTIAL PROTEIN SEQUENCE.

    Entry informationi

    Entry nameiT1SK_ECOLI
    AccessioniPrimary (citable) accession number: P05719
    Secondary accession number(s): Q2M5W8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1988
    Last sequence update: November 1, 1988
    Last modified: October 1, 2014
    This is version 116 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3