Reviewed,
UniProtKB/Swiss-Prot P05719 (T1SK_ECOLI)
Last modified
June 16, 2009.
Version 75.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Type-1 restriction enzyme EcoKI specificity protein Short name=S.EcoKI Alternative name(s): Type I restriction enzyme EcoKI specificity protein Short name=S protein | ||||||
| Gene names |
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| Organism | Escherichia coli (strain K12) [Complete proteome] [HAMAP] | ||||||
| Taxonomic identifier | 83333 [NCBI] | ||||||
| Taxonomic lineage | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia |
Protein attributes
| Sequence length | 464 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to the same target sequence but cutting the DNA some distance from this site. Whether the DNA is cut or modified depends on the methylation state of the target sequence. When the target site is unmodified, the DNA is cut. When the target site is hemimethylated, the complex acts as a maintenance MTase modifying the DNA so that both strands become methylated. Subunit S dictates DNA sequences specificity. The EcoKI enzyme recognizes 5'-AACN6GTGC-3'. |
| Subunit structure | The type I restriction/modification system is composed of three polypeptides R, M and S. |
| Domain | Contains two DNA recognition domains, each specifying recognition of one of the two defined components of the target sequence. |
| Sequence similarities | Belongs to the type-I restriction system S methylase family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Restriction system |
| Ligand | DNA-binding |
| Technical term | Complete proteome Direct protein sequencing |
| Gene Ontology (GO) | |
| Biological process | DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | DNA binding Inferred from electronic annotation. Source: UniProtKB-KW protein bindingInferred from physical interaction. Source: IntAct |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||
Molecule processing | |||||||
|---|---|---|---|---|---|---|---|
| Chain | 1 – 464 | 464 | Type-1 restriction enzyme EcoKI specificity protein | PRO_0000198035 | |||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Sequence diversity among related genes for recognition of specific targets in DNA molecules." Gough J.A., Murray N.E. J. Mol. Biol. 166:1-19(1983) [PubMed: 6304321] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12. |
| [2] | "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R. Nucleic Acids Res. 23:2105-2119(1995) [PubMed: 7610040] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076. |
| [3] | "The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076. |
| [4] | "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911. |
| [5] | "Tyrosine 27 of the specificity polypeptide of EcoKI can be UV crosslinked to a bromodeoxyuridine-substituted DNA target sequence." Chen A., Powell L.M., Dryden D.T., Murray N.E., Brown T. Nucleic Acids Res. 23:1177-1183(1995) [PubMed: 7739896] [Abstract] Cited for: PARTIAL PROTEIN SEQUENCE. |
Cross-references
Sequence databases | |
|---|---|
| V00288 Genomic DNA. Translation: CAA23554.1. U14003 Genomic DNA. Translation: AAA97245.1. U00096 Genomic DNA. Translation: AAC77304.1. AP009048 Genomic DNA. Translation: BAE78338.1. | |
| PIR | NDECKS. A30369. |
| RefSeq | AP_004837.1. NP_418768.1. |
3D structure databases | |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | P05719. 1 interaction. |
Protein family/group databases | |
| REBASE | 3646. S.EcoKI. |
Genome annotation databases | |
| GeneID | 948867. |
| GenomeReviews | Gene locus JW4311 in contig AP009048_GR. Gene locus b4348 in contig U00096_GR. |
| KEGG | ecj:JW4311. eco:b4348. |
Organism-specific databases | |
| EchoBASE | EB0455. |
| EcoGene | EG10460. hsdS. |
| CMR | Search... |
Phylogenomic databases | |
| HOGENOM | P05719. |
| OMA | P05719. AQVDTLK. |
Enzyme and pathway databases | |
| BioCyc | EcoCyc:EG10460-MON. |
Family and domain databases | |
| InterPro | IPR000055. Restrict_endonuc_I_S_EcoBI. [Graphical view] |
| Pfam | PF01420. Methylase_S. 2 hits. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | T1SK_ECOLI | ||||||||
| Accession | Primary (citable) accession number: P05719 Secondary accession number(s): Q2M5W8 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||
Relevant documents
| Escherichia coli Escherichia coli (strain K12): entries and cross-references to EcoGene |
| Restriction enzymes and methylases Classification of restriction enzymes and methylases and list of entries |
| SIMILARITY comments Index of protein domains and families |

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