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P05719

- T1SK_ECOLI

UniProt

P05719 - T1SK_ECOLI

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Protein

Type-1 restriction enzyme EcoKI specificity protein

Gene

hsdS

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to the same target sequence but cutting the DNA some distance from this site. Whether the DNA is cut or modified depends on the methylation state of the target sequence. When the target site is unmodified, the DNA is cut. When the target site is hemimethylated, the complex acts as a maintenance MTase modifying the DNA so that both strands become methylated. Subunit S dictates DNA sequences specificity. The EcoKI enzyme recognizes 5'-AACN6GTGC-3'.

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Restriction system

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG10460-MONOMER.
ECOL316407:JW4311-MONOMER.
MetaCyc:EG10460-MONOMER.

Protein family/group databases

REBASEi3646. S.EcoKI.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-1 restriction enzyme EcoKI specificity protein
Short name:
S.EcoKI
Alternative name(s):
Type I restriction enzyme EcoKI specificity protein
Short name:
S protein
Gene namesi
Name:hsdS
Synonyms:hss
Ordered Locus Names:b4348, JW4311
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG10460. hsdS.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 464464Type-1 restriction enzyme EcoKI specificity proteinPRO_0000198035Add
BLAST

Proteomic databases

PaxDbiP05719.
PRIDEiP05719.

Expressioni

Gene expression databases

GenevestigatoriP05719.

Interactioni

Subunit structurei

The type I restriction/modification system is composed of three polypeptides R, M and S.

Binary interactionsi

WithEntry#Exp.IntActNotes
ftsHP0AAI31EBI-1135164,EBI-548381

Protein-protein interaction databases

DIPiDIP-9945N.
IntActiP05719. 1 interaction.
MINTiMINT-1225773.
STRINGi511145.b4348.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2Y7Celectron microscopy18.00A1-464[»]
2Y7Helectron microscopy18.00A1-464[»]
ProteinModelPortaliP05719.
SMRiP05719. Positions 1-464.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP05719.

Family & Domainsi

Domaini

Contains two DNA recognition domains, each specifying recognition of one of the two defined components of the target sequence.

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0732.
HOGENOMiHOG000295029.
InParanoidiP05719.
KOiK01154.
OMAiMLAIDAC.
OrthoDBiEOG62ZHW5.
PhylomeDBiP05719.

Family and domain databases

InterProiIPR000055. Restrct_endonuc_typeI_HsdS.
[Graphical view]
PfamiPF01420. Methylase_S. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P05719-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSAGKLPEGW VIAPVSTVTT LIRGVTYKKE QAINYLKDDY LPLIRANNIQ
60 70 80 90 100
NGKFDTTDLV FVPKNLVKES QKISPEDIVI AMSSGSKSVV GKSAHQHLPF
110 120 130 140 150
ECSFGAFCGV LRPEKLIFSG FIAHFTKSSL YRNKISSLSA GANINNIKPA
160 170 180 190 200
SFDLINIPIP PLAEQKIIAE KLDTLLAQVD STKARFEQIP QILKRFRQAV
210 220 230 240 250
LGGAVNGKLT EKWRNFEPQH SVFKKLNFES ILTELRNGLS SKPNESGVGH
260 270 280 290 300
PILRISSVRA GHVDQNDIRF LECSESELNR HKLQDGDLLF TRYNGSLEFV
310 320 330 340 350
GVCGLLKKLQ HQNLLYPDKL IRARLTKDAL PEYIEIFFSS PSARNAMMNC
360 370 380 390 400
VKTTSGQKGI SGKDIKSQVV LLPPVKEQAE IVRRVEQLFA YADTIEKQVN
410 420 430 440 450
NALARVNNLT QSILAKAFRG ELTAQWRAEN PDLISGENSA AALLEKIKAE
460
RAASGGKKAS RKKS
Length:464
Mass (Da):51,395
Last modified:November 1, 1988 - v1
Checksum:i1D651D0716B08C04
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00288 Genomic DNA. Translation: CAA23554.1.
U14003 Genomic DNA. Translation: AAA97245.1.
U00096 Genomic DNA. Translation: AAC77304.1.
AP009048 Genomic DNA. Translation: BAE78338.1.
PIRiA30369. NDECKS.
RefSeqiNP_418768.1. NC_000913.3.
YP_492479.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC77304; AAC77304; b4348.
BAE78338; BAE78338; BAE78338.
GeneIDi12933773.
948867.
KEGGiecj:Y75_p4233.
eco:b4348.
PATRICi32124302. VBIEscCol129921_4494.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00288 Genomic DNA. Translation: CAA23554.1 .
U14003 Genomic DNA. Translation: AAA97245.1 .
U00096 Genomic DNA. Translation: AAC77304.1 .
AP009048 Genomic DNA. Translation: BAE78338.1 .
PIRi A30369. NDECKS.
RefSeqi NP_418768.1. NC_000913.3.
YP_492479.1. NC_007779.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2Y7C electron microscopy 18.00 A 1-464 [» ]
2Y7H electron microscopy 18.00 A 1-464 [» ]
ProteinModelPortali P05719.
SMRi P05719. Positions 1-464.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-9945N.
IntActi P05719. 1 interaction.
MINTi MINT-1225773.
STRINGi 511145.b4348.

Protein family/group databases

REBASEi 3646. S.EcoKI.

Proteomic databases

PaxDbi P05719.
PRIDEi P05719.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC77304 ; AAC77304 ; b4348 .
BAE78338 ; BAE78338 ; BAE78338 .
GeneIDi 12933773.
948867.
KEGGi ecj:Y75_p4233.
eco:b4348.
PATRICi 32124302. VBIEscCol129921_4494.

Organism-specific databases

EchoBASEi EB0455.
EcoGenei EG10460. hsdS.

Phylogenomic databases

eggNOGi COG0732.
HOGENOMi HOG000295029.
InParanoidi P05719.
KOi K01154.
OMAi MLAIDAC.
OrthoDBi EOG62ZHW5.
PhylomeDBi P05719.

Enzyme and pathway databases

BioCyci EcoCyc:EG10460-MONOMER.
ECOL316407:JW4311-MONOMER.
MetaCyc:EG10460-MONOMER.

Miscellaneous databases

EvolutionaryTracei P05719.
PROi P05719.

Gene expression databases

Genevestigatori P05719.

Family and domain databases

InterProi IPR000055. Restrct_endonuc_typeI_HsdS.
[Graphical view ]
Pfami PF01420. Methylase_S. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence diversity among related genes for recognition of specific targets in DNA molecules."
    Gough J.A., Murray N.E.
    J. Mol. Biol. 166:1-19(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes."
    Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.
    Nucleic Acids Res. 23:2105-2119(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Tyrosine 27 of the specificity polypeptide of EcoKI can be UV crosslinked to a bromodeoxyuridine-substituted DNA target sequence."
    Chen A., Powell L.M., Dryden D.T., Murray N.E., Brown T.
    Nucleic Acids Res. 23:1177-1183(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL PROTEIN SEQUENCE.

Entry informationi

Entry nameiT1SK_ECOLI
AccessioniPrimary (citable) accession number: P05719
Secondary accession number(s): Q2M5W8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: November 1, 1988
Last modified: October 29, 2014
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3