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P05719 (T1SK_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 115. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type-1 restriction enzyme EcoKI specificity protein

Short name=S.EcoKI
Alternative name(s):
Type I restriction enzyme EcoKI specificity protein
Short name=S protein
Gene names
Name:hsdS
Synonyms:hss
Ordered Locus Names:b4348, JW4311
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length464 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to the same target sequence but cutting the DNA some distance from this site. Whether the DNA is cut or modified depends on the methylation state of the target sequence. When the target site is unmodified, the DNA is cut. When the target site is hemimethylated, the complex acts as a maintenance MTase modifying the DNA so that both strands become methylated. Subunit S dictates DNA sequences specificity. The EcoKI enzyme recognizes 5'-AACN6GTGC-3'.

Subunit structure

The type I restriction/modification system is composed of three polypeptides R, M and S.

Domain

Contains two DNA recognition domains, each specifying recognition of one of the two defined components of the target sequence.

Sequence similarities

Belongs to the type-I restriction system S methylase family.

Ontologies

Keywords
   Biological processRestriction system
   LigandDNA-binding
   Technical term3D-structure
Complete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

ftsHP0AAI31EBI-1135164,EBI-548381

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 464464Type-1 restriction enzyme EcoKI specificity protein
PRO_0000198035

Sequences

Sequence LengthMass (Da)Tools
P05719 [UniParc].

Last modified November 1, 1988. Version 1.
Checksum: 1D651D0716B08C04

FASTA46451,395
        10         20         30         40         50         60 
MSAGKLPEGW VIAPVSTVTT LIRGVTYKKE QAINYLKDDY LPLIRANNIQ NGKFDTTDLV 

        70         80         90        100        110        120 
FVPKNLVKES QKISPEDIVI AMSSGSKSVV GKSAHQHLPF ECSFGAFCGV LRPEKLIFSG 

       130        140        150        160        170        180 
FIAHFTKSSL YRNKISSLSA GANINNIKPA SFDLINIPIP PLAEQKIIAE KLDTLLAQVD 

       190        200        210        220        230        240 
STKARFEQIP QILKRFRQAV LGGAVNGKLT EKWRNFEPQH SVFKKLNFES ILTELRNGLS 

       250        260        270        280        290        300 
SKPNESGVGH PILRISSVRA GHVDQNDIRF LECSESELNR HKLQDGDLLF TRYNGSLEFV 

       310        320        330        340        350        360 
GVCGLLKKLQ HQNLLYPDKL IRARLTKDAL PEYIEIFFSS PSARNAMMNC VKTTSGQKGI 

       370        380        390        400        410        420 
SGKDIKSQVV LLPPVKEQAE IVRRVEQLFA YADTIEKQVN NALARVNNLT QSILAKAFRG 

       430        440        450        460 
ELTAQWRAEN PDLISGENSA AALLEKIKAE RAASGGKKAS RKKS 

« Hide

References

« Hide 'large scale' references
[1]"Sequence diversity among related genes for recognition of specific targets in DNA molecules."
Gough J.A., Murray N.E.
J. Mol. Biol. 166:1-19(1983) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[2]"Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes."
Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.
Nucleic Acids Res. 23:2105-2119(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"Tyrosine 27 of the specificity polypeptide of EcoKI can be UV crosslinked to a bromodeoxyuridine-substituted DNA target sequence."
Chen A., Powell L.M., Dryden D.T., Murray N.E., Brown T.
Nucleic Acids Res. 23:1177-1183(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: PARTIAL PROTEIN SEQUENCE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
V00288 Genomic DNA. Translation: CAA23554.1.
U14003 Genomic DNA. Translation: AAA97245.1.
U00096 Genomic DNA. Translation: AAC77304.1.
AP009048 Genomic DNA. Translation: BAE78338.1.
PIRNDECKS. A30369.
RefSeqNP_418768.1. NC_000913.3.
YP_492479.1. NC_007779.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2Y7Celectron microscopy18.00A1-464[»]
2Y7Helectron microscopy18.00A1-464[»]
ProteinModelPortalP05719.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-9945N.
IntActP05719. 1 interaction.
MINTMINT-1225773.
STRING511145.b4348.

Protein family/group databases

REBASE3646. S.EcoKI.

Proteomic databases

PaxDbP05719.
PRIDEP05719.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC77304; AAC77304; b4348.
BAE78338; BAE78338; BAE78338.
GeneID12933773.
948867.
KEGGecj:Y75_p4233.
eco:b4348.
PATRIC32124302. VBIEscCol129921_4494.

Organism-specific databases

EchoBASEEB0455.
EcoGeneEG10460. hsdS.

Phylogenomic databases

eggNOGCOG0732.
HOGENOMHOG000295029.
KOK01154.
OMAMLAIDAC.
OrthoDBEOG62ZHW5.
PhylomeDBP05719.

Enzyme and pathway databases

BioCycEcoCyc:EG10460-MONOMER.
ECOL316407:JW4311-MONOMER.
MetaCyc:EG10460-MONOMER.

Gene expression databases

GenevestigatorP05719.

Family and domain databases

InterProIPR000055. Restrct_endonuc_typeI_HsdS.
[Graphical view]
PfamPF01420. Methylase_S. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP05719.
PROP05719.

Entry information

Entry nameT1SK_ECOLI
AccessionPrimary (citable) accession number: P05719
Secondary accession number(s): Q2M5W8
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: November 1, 1988
Last modified: June 11, 2014
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene