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P05714 (RAB4A_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 126. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ras-related protein Rab-4A
Gene names
Name:Rab4a
Synonyms:Rab4
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length218 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Protein transport. Probably involved in vesicular traffic By similarity.

Subunit structure

Interacts with RAB11FIP1, RABEP1, ZFYVE20 and RUFY1. Interacts with SGSM1, SGSM2 and SGSM3. Interacts (membrane-bound form) with NDRG1; the interaction involves NDRG1 in vesicular recycling of E-cadherin By similarity.

Subcellular location

Membrane; Peripheral membrane protein By similarity. Cytoplasm By similarity. Note: Generally associated with membranes. Cytoplasmic when phosphorylated by CDK1.

Post-translational modification

Phosphorylated by CDK1 kinase during mitosis By similarity.

Sequence similarities

Belongs to the small GTPase superfamily. Rab family.

Sequence caution

The sequence CAA30006.1 differs from that shown. Reason: Frameshift at position 3.

Ontologies

Keywords
   Biological processProtein transport
Transport
   Cellular componentCytoplasm
Membrane
   LigandGTP-binding
Nucleotide-binding
   PTMLipoprotein
Methylation
Phosphoprotein
Prenylation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processGTP catabolic process

Inferred from direct assay PubMed 11063739PubMed 9169411. Source: GOC

Rab protein signal transduction

Inferred from mutant phenotype PubMed 9169411. Source: RGD

positive regulation of ATPase activity

Inferred from direct assay PubMed 11062069. Source: GOC

protein transport

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytosol

Inferred from direct assay PubMed 7575448PubMed 8037667PubMed 8366094. Source: RGD

insulin-responsive compartment

Inferred from direct assay PubMed 8366094. Source: RGD

intracellular membrane-bounded organelle

Inferred from direct assay PubMed 11063739PubMed 8037667PubMed 8366094PubMed 8536622PubMed 9169411. Source: RGD

membrane

Inferred from direct assay PubMed 8807639. Source: RGD

plasma membrane

Inferred from direct assay PubMed 11063739PubMed 7575448PubMed 8037667PubMed 8366094. Source: RGD

recycling endosome

Inferred from direct assay PubMed 10468577. Source: RGD

   Molecular_functionATPase activator activity

Inferred from direct assay PubMed 11062069. Source: RGD

ATPase binding

Inferred from direct assay PubMed 11062069. Source: RGD

GDP binding

Inferred from sequence or structural similarity. Source: UniProtKB

GTP binding

Inferred from sequence or structural similarity. Source: UniProtKB

GTPase activity

Inferred from sequence or structural similarity. Source: UniProtKB

ionotropic glutamate receptor binding

Inferred from physical interaction PubMed 17194442. Source: RGD

syntaxin binding

Inferred from physical interaction PubMed 11063739. Source: RGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 218218Ras-related protein Rab-4A
PRO_0000121095

Regions

Nucleotide binding20 – 289GTP By similarity
Nucleotide binding68 – 725GTP By similarity
Nucleotide binding126 – 1294GTP By similarity
Nucleotide binding156 – 1583GTP By similarity
Motif42 – 509Effector region By similarity

Amino acid modifications

Modified residue2041Phosphoserine; by CDK1 By similarity
Modified residue2181Cysteine methyl ester By similarity
Lipidation2161S-geranylgeranyl cysteine By similarity
Lipidation2181S-geranylgeranyl cysteine By similarity

Experimental info

Sequence conflict521S → Q in CAA30006. Ref.1
Sequence conflict801R → T in CAA30006. Ref.1
Sequence conflict1571A → P in CAA30006. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P05714 [UniParc].

Last modified March 6, 2013. Version 3.
Checksum: A576827D571CAD01

FASTA21824,409
        10         20         30         40         50         60 
MAQTAMSETY DFLFKFLVIG NAGTGKSCLL HQFIEKKFKD DSNHTIGVEF GSKIINVGGK 

        70         80         90        100        110        120 
YVKLQIWDTA GQERFRSVTR SYYRGAAGAL LVYDITSRET YNALTNWLTD ARMLASQNIV 

       130        140        150        160        170        180 
IILCGNKKDL DADREVTFLE ASRFAQENEL MFLETSALTG ENVEEAFMQC ARKILNKIES 

       190        200        210 
GELDPERMGS GIQYGDAALR QLRSPRRTQA PSAQECGC 

« Hide

References

« Hide 'large scale' references
[1]"Complete coding sequences of the ras related rab 3 and 4 cDNAs."
Zahraoui A., Touchot N., Chardin P., Tavitian A.
Nucleic Acids Res. 16:1204-1204(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Prostate.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X06890 mRNA. Translation: CAA30006.1. Frameshift.
BC062016 mRNA. Translation: AAH62016.1.
PIRS01766.
RefSeqNP_037151.2. NM_013019.2.
UniGeneRn.3016.

3D structure databases

ProteinModelPortalP05714.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000023771.

Proteomic databases

PaxDbP05714.
PRIDEP05714.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID25532.
KEGGrno:25532.
UCSCRGD:3529. rat.

Organism-specific databases

CTD5867.
RGD3529. Rab4a.

Phylogenomic databases

eggNOGCOG1100.
HOGENOMHOG000233968.
HOVERGENHBG009351.
InParanoidP05714.
KOK07879.
TreeFamTF300032.

Gene expression databases

GenevestigatorP05714.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view]
PfamPF00071. Ras. 1 hit.
[Graphical view]
PRINTSPR00449. RASTRNSFRMNG.
SMARTSM00175. RAB. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR00231. small_GTP. 1 hit.
PROSITEPS51419. RAB. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio607033.
PROP05714.

Entry information

Entry nameRAB4A_RAT
AccessionPrimary (citable) accession number: P05714
Secondary accession number(s): Q6P6U4
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: March 6, 2013
Last modified: April 16, 2014
This is version 126 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families