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Protein

Protein single-minded

Gene

sim

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcription factor that functions as a master developmental regulator controlling midline development of the ventral nerve cord. Required to correctly specify the formation of the central brain complex, which controls walking behavior. Also required for correct patterning of the embryonic genital disk and anal pad anlage. Plays a role in synapse development.3 Publications

Miscellaneous

Mutations result in the loss of the precursor cells that give rise to midline cells of the embryonic central nervous system.

GO - Molecular functioni

  • protein heterodimerization activity Source: FlyBase
  • RNA polymerase II proximal promoter sequence-specific DNA binding Source: FlyBase
  • sequence-specific DNA binding Source: FlyBase
  • transcription factor activity, RNA polymerase II proximal promoter sequence-specific DNA binding Source: FlyBase

GO - Biological processi

  • adult walking behavior Source: FlyBase
  • axon guidance Source: FlyBase
  • axonogenesis Source: FlyBase
  • brain development Source: FlyBase
  • determination of genital disc primordium Source: FlyBase
  • ectoderm development Source: FlyBase
  • locomotion Source: FlyBase
  • positive regulation of transcription by RNA polymerase II Source: FlyBase
  • regulation of transcription, DNA-templated Source: UniProtKB
  • transcription by RNA polymerase II Source: GOC
  • ventral cord development Source: FlyBase
  • ventral midline development Source: UniProtKB

Keywordsi

Molecular functionDevelopmental protein, DNA-binding
Biological processDifferentiation, Neurogenesis, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Protein single-minded
Gene namesi
Name:sim
ORF Names:CG7771
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0004666 sim

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi65S → F in allele sim-J1-47; temperature sensitive embryonic midline axon phenotype. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001274381 – 697Protein single-mindedAdd BLAST697

Proteomic databases

PaxDbiP05709
PRIDEiP05709

Expressioni

Tissue specificityi

Embryonic nerve cord.1 Publication

Gene expression databases

BgeeiFBgn0004666
GenevisibleiP05709 DM

Interactioni

Subunit structurei

Efficient DNA binding requires dimerization with another bHLH protein.

GO - Molecular functioni

  • protein heterodimerization activity Source: FlyBase

Protein-protein interaction databases

BioGridi66699, 8 interactors
IntActiP05709, 3 interactors
STRINGi7227.FBpp0082178

Structurei

3D structure databases

ProteinModelPortaliP05709
SMRiP05709
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini24 – 77bHLHPROSITE-ProRule annotationAdd BLAST54
Domaini100 – 172PAS 1PROSITE-ProRule annotationAdd BLAST73
Domaini266 – 336PAS 2PROSITE-ProRule annotationAdd BLAST71

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni406 – 44614 X 3 AA repeats of A-A-Q (approximate)Add BLAST41

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi553 – 672Ser-richAdd BLAST120
Compositional biasi673 – 693Gln/His-richAdd BLAST21

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG3559 Eukaryota
ENOG410XY57 LUCA
InParanoidiP05709
KOiK09100
OrthoDBiEOG091G06FQ
PhylomeDBiP05709

Family and domain databases

CDDicd00083 HLH, 1 hit
cd00130 PAS, 2 hits
Gene3Di4.10.280.10, 1 hit
InterProiView protein in InterPro
IPR011598 bHLH_dom
IPR036638 HLH_DNA-bd_sf
IPR001067 Nuc_translocat
IPR001610 PAC
IPR000014 PAS
IPR035965 PAS-like_dom_sf
IPR013767 PAS_fold
IPR013655 PAS_fold_3
PfamiView protein in Pfam
PF00010 HLH, 1 hit
PF00989 PAS, 1 hit
PF08447 PAS_3, 1 hit
PRINTSiPR00785 NCTRNSLOCATR
SMARTiView protein in SMART
SM00353 HLH, 1 hit
SM00086 PAC, 1 hit
SM00091 PAS, 2 hits
SUPFAMiSSF47459 SSF47459, 1 hit
SSF55785 SSF55785, 2 hits
TIGRFAMsiTIGR00229 sensory_box, 1 hit
PROSITEiView protein in PROSITE
PS50888 BHLH, 1 hit
PS50112 PAS, 2 hits

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform A (identifier: P05709-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTNHRRVRKD CYESRLHDIA KTCAMKEKSK NAARTRREKE NTEFCELAKL
60 70 80 90 100
LPLPAAITSQ LDKASVIRLT TSYLKMRQVF PDGLGEAWGS SPAMQRGATI
110 120 130 140 150
KELGSHLLQT LDGFIFVVAP DGKIMYISET ASVHLGLSQV ELTGNSIFEY
160 170 180 190 200
IHNYDQDEMN AILSLHPHIN QHPLAQTHTP IGSPNGVQHP SAYDHDRGSH
210 220 230 240 250
TIEIEKTFFL RMKCVLAKRN AGLTTSGFKV IHCSGYLKAR IYPDRGDGQG
260 270 280 290 300
SLIQNLGLVA VGHSLPSSAI TEIKLHQNMF MFRAKLDMKL IFFDARVSQL
310 320 330 340 350
TGYEPQDLIE KTLYQYIHAA DIMAMRCSHQ ILLYKGQVTT KYYRFLTKGG
360 370 380 390 400
GWVWVQSYAT LVHNSRSSRE VFIVSVNYVL SEREVKDLVL NEIQTGVVKR
410 420 430 440 450
EPISPAAQAA QAAQAAQAAQ AAQAAQAAQA AQAAQAAHVA QAVQAQVVVV
460 470 480 490 500
PQQSVVVQPQ CAGATGQPVG PGTPVSLALS ASPKLDPYFE PELPLQPAVT
510 520 530 540 550
PVPPTNNSSS SSNNNNGVWH HHHVQQQQQS GSMDHDSLSY TQLYPPLNDL
560 570 580 590 600
VVSSSSSVGG GTASSAGGGS SASASSSGVY STEMQYPDTT TGNLYYNNNN
610 620 630 640 650
HYYYDYDATV DVATSMIRPF SANSNSCSSS SESERQLSTG NASIVNETSP
660 670 680 690
SQTTYSDLSH NFELSYFSDN SSQQHQHQQQ QQHLMEQQHL QYQYATW
Note: No experimental confirmation available.
Length:697
Mass (Da):76,475
Last modified:May 23, 2003 - v3
Checksum:i588414A4A17101AD
GO
Isoform B (identifier: P05709-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-24: Missing.

Show »
Length:673
Mass (Da):73,589
Checksum:i2F9F0ABBA2BC0FBE
GO

Sequence cautioni

The sequence AAC64519 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti151I → Y in AAC64519 (PubMed:9840810).Curated1

Polymorphismi

Berkeley strain has 11 A-A-Q repeats.1 Publication

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti406 – 414Missing in strain: Berkeley. 9

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0118121 – 24Missing in isoform B. CuratedAdd BLAST24

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF071934 Genomic DNA Translation: AAC64519.1 Sequence problems.
AE014297 Genomic DNA Translation: AAF54902.3
AE014297 Genomic DNA Translation: AAN14343.3
AY129457 mRNA Translation: AAM76199.1
M19020 mRNA Translation: AAA28900.1
PIRiA29945
A41647
RefSeqiNP_524340.2, NM_079616.4
NP_731771.3, NM_169495.4
UniGeneiDm.4557

Genome annotation databases

GeneIDi41612
KEGGidme:Dmel_CG7771

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiSIM_DROME
AccessioniPrimary (citable) accession number: P05709
Secondary accession number(s): O96521
, Q7KSL7, Q8MQI7, Q9VFZ3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: May 23, 2003
Last modified: March 28, 2018
This is version 181 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health