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Protein

Sorbitol-6-phosphate 2-dehydrogenase

Gene

srlD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

D-sorbitol 6-phosphate + NAD+ = D-fructose 6-phosphate + NADH.

Pathwayi: D-sorbitol degradation

This protein is involved in step 1 of the subpathway that synthesizes D-fructose 6-phosphate from D-sorbitol 6-phosphate.
Proteins known to be involved in this subpathway in this organism are:
  1. Sorbitol-6-phosphate 2-dehydrogenase (srlD)
This subpathway is part of the pathway D-sorbitol degradation, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-fructose 6-phosphate from D-sorbitol 6-phosphate, the pathway D-sorbitol degradation and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei141 – 1411SubstrateBy similarity
Active sitei154 – 1541Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi4 – 3330NADBy similarityAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciEcoCyc:SORB6PDEHYDROG-MONOMER.
ECOL316407:JW2674-MONOMER.
MetaCyc:SORB6PDEHYDROG-MONOMER.
UniPathwayiUPA00812; UER00783.

Names & Taxonomyi

Protein namesi
Recommended name:
Sorbitol-6-phosphate 2-dehydrogenase (EC:1.1.1.140)
Alternative name(s):
Glucitol-6-phosphate dehydrogenase
Ketosephosphate reductase
Gene namesi
Name:srlD
Synonyms:gutD
Ordered Locus Names:b2705, JW2674
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10971. srlD.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 259259Sorbitol-6-phosphate 2-dehydrogenasePRO_0000054779Add
BLAST

Proteomic databases

PaxDbiP05707.

Interactioni

Subunit structurei

Homotetramer.

Protein-protein interaction databases

BioGridi4260703. 10 interactions.
853206. 2 interactions.
DIPiDIP-10917N.
IntActiP05707. 3 interactions.
STRINGi511145.b2705.

Structurei

3D structure databases

ProteinModelPortaliP05707.
SMRiP05707. Positions 1-258.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4107QMF. Bacteria.
COG1028. LUCA.
InParanoidiP05707.
KOiK00068.
OMAiINTEFGE.
OrthoDBiEOG6N3CR8.
PhylomeDBiP05707.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 2 hits.
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P05707-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNQVAVVIGG GQTLGAFLCH GLAAEGYRVA VVDIQSDKAA NVAQEINAEY
60 70 80 90 100
GESMAYGFGA DATSEQSVLA LSRGVDEIFG RVDLLVYSAG IAKAAFISDF
110 120 130 140 150
QLGDFDRSLQ VNLVGYFLCA REFSRLMIRD GIQGRIIQIN SKSGKVGSKH
160 170 180 190 200
NSGYSAAKFG GVGLTQSLAL DLAEYGITVH SLMLGNLLKS PMFQSLLPQY
210 220 230 240 250
ATKLGIKPDQ VEQYYIDKVP LKRGCDYQDV LNMLLFYASP KASYCTGQSI

NVTGGQVMF
Length:259
Mass (Da):27,858
Last modified:October 1, 1996 - v2
Checksum:i29C6215F857B5FBC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti68 – 681V → C in AAC13413 (PubMed:3553176).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02708 Genomic DNA. Translation: AAC13413.1.
U29579 Genomic DNA. Translation: AAA69214.1.
U00096 Genomic DNA. Translation: AAC75747.1.
AP009048 Genomic DNA. Translation: BAA16566.1.
PIRiE65050. DEECSP.
RefSeqiNP_417185.1. NC_000913.3.
WP_001077358.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75747; AAC75747; b2705.
BAA16566; BAA16566; BAA16566.
GeneIDi948937.
KEGGiecj:JW2674.
eco:b2705.
PATRICi32120808. VBIEscCol129921_2796.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02708 Genomic DNA. Translation: AAC13413.1.
U29579 Genomic DNA. Translation: AAA69214.1.
U00096 Genomic DNA. Translation: AAC75747.1.
AP009048 Genomic DNA. Translation: BAA16566.1.
PIRiE65050. DEECSP.
RefSeqiNP_417185.1. NC_000913.3.
WP_001077358.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP05707.
SMRiP05707. Positions 1-258.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260703. 10 interactions.
853206. 2 interactions.
DIPiDIP-10917N.
IntActiP05707. 3 interactions.
STRINGi511145.b2705.

Proteomic databases

PaxDbiP05707.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75747; AAC75747; b2705.
BAA16566; BAA16566; BAA16566.
GeneIDi948937.
KEGGiecj:JW2674.
eco:b2705.
PATRICi32120808. VBIEscCol129921_2796.

Organism-specific databases

EchoBASEiEB0964.
EcoGeneiEG10971. srlD.

Phylogenomic databases

eggNOGiENOG4107QMF. Bacteria.
COG1028. LUCA.
InParanoidiP05707.
KOiK00068.
OMAiINTEFGE.
OrthoDBiEOG6N3CR8.
PhylomeDBiP05707.

Enzyme and pathway databases

UniPathwayiUPA00812; UER00783.
BioCyciEcoCyc:SORB6PDEHYDROG-MONOMER.
ECOL316407:JW2674-MONOMER.
MetaCyc:SORB6PDEHYDROG-MONOMER.

Miscellaneous databases

PROiP05707.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 2 hits.
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Glucitol-specific enzymes of the phosphotransferase system in Escherichia coli. Nucleotide sequence of the gut operon."
    Yamada M., Saier M.H. Jr.
    J. Biol. Chem. 262:5455-5463(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features."
    Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T.
    , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.
    DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Purification and properties of D-mannitol-1-phosphate dehydrogenase and D-glucitol-6-phosphate dehydrogenase from Escherichia coli."
    Novotny M.J., Reizer J., Esch F., Saier M.H. Jr.
    J. Bacteriol. 159:986-990(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-34.

Entry informationi

Entry nameiSRLD_ECOLI
AccessioniPrimary (citable) accession number: P05707
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: October 1, 1996
Last modified: April 13, 2016
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.