Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Sorbitol-6-phosphate 2-dehydrogenase

Gene

srlD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

D-sorbitol 6-phosphate + NAD+ = D-fructose 6-phosphate + NADH.

Pathwayi: D-sorbitol degradation

This protein is involved in step 1 of the subpathway that synthesizes D-fructose 6-phosphate from D-sorbitol 6-phosphate.
Proteins known to be involved in this subpathway in this organism are:
  1. Sorbitol-6-phosphate 2-dehydrogenase (srlD)
This subpathway is part of the pathway D-sorbitol degradation, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-fructose 6-phosphate from D-sorbitol 6-phosphate, the pathway D-sorbitol degradation and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei141SubstrateBy similarity1
Active sitei154Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi4 – 33NADBy similarityAdd BLAST30

GO - Molecular functioni

  • identical protein binding Source: EcoCyc
  • sorbitol-6-phosphate 2-dehydrogenase activity Source: EcoCyc

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
LigandNAD

Enzyme and pathway databases

BioCyciEcoCyc:SORB6PDEHYDROG-MONOMER.
MetaCyc:SORB6PDEHYDROG-MONOMER.
UniPathwayiUPA00812; UER00783.

Names & Taxonomyi

Protein namesi
Recommended name:
Sorbitol-6-phosphate 2-dehydrogenase (EC:1.1.1.140)
Alternative name(s):
Glucitol-6-phosphate dehydrogenase
Ketosephosphate reductase
Gene namesi
Name:srlD
Synonyms:gutD
Ordered Locus Names:b2705, JW2674
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10971. srlD.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000547791 – 259Sorbitol-6-phosphate 2-dehydrogenaseAdd BLAST259

Proteomic databases

PaxDbiP05707.
PRIDEiP05707.

Interactioni

Subunit structurei

Homotetramer.

GO - Molecular functioni

  • identical protein binding Source: EcoCyc

Protein-protein interaction databases

BioGridi4260703. 10 interactors.
853206. 2 interactors.
DIPiDIP-10917N.
IntActiP05707. 3 interactors.
STRINGi316385.ECDH10B_2873.

Structurei

3D structure databases

ProteinModelPortaliP05707.
SMRiP05707.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4107QMF. Bacteria.
COG1028. LUCA.
InParanoidiP05707.
KOiK00068.
PhylomeDBiP05707.

Family and domain databases

InterProiView protein in InterPro
IPR036291. NAD(P)-bd_dom_sf.
IPR020904. Sc_DH/Rdtase_CS.
IPR002347. SDR_fam.
PfamiView protein in Pfam
PF00106. adh_short. 1 hit.
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiView protein in PROSITE
PS00061. ADH_SHORT. 1 hit.

Sequencei

Sequence statusi: Complete.

P05707-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNQVAVVIGG GQTLGAFLCH GLAAEGYRVA VVDIQSDKAA NVAQEINAEY
60 70 80 90 100
GESMAYGFGA DATSEQSVLA LSRGVDEIFG RVDLLVYSAG IAKAAFISDF
110 120 130 140 150
QLGDFDRSLQ VNLVGYFLCA REFSRLMIRD GIQGRIIQIN SKSGKVGSKH
160 170 180 190 200
NSGYSAAKFG GVGLTQSLAL DLAEYGITVH SLMLGNLLKS PMFQSLLPQY
210 220 230 240 250
ATKLGIKPDQ VEQYYIDKVP LKRGCDYQDV LNMLLFYASP KASYCTGQSI

NVTGGQVMF
Length:259
Mass (Da):27,858
Last modified:October 1, 1996 - v2
Checksum:i29C6215F857B5FBC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti68V → C in AAC13413 (PubMed:3553176).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02708 Genomic DNA. Translation: AAC13413.1.
U29579 Genomic DNA. Translation: AAA69214.1.
U00096 Genomic DNA. Translation: AAC75747.1.
AP009048 Genomic DNA. Translation: BAA16566.1.
PIRiE65050. DEECSP.
RefSeqiNP_417185.1. NC_000913.3.
WP_001077358.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75747; AAC75747; b2705.
BAA16566; BAA16566; BAA16566.
GeneIDi948937.
KEGGiecj:JW2674.
eco:b2705.
PATRICifig|1411691.4.peg.4037.

Similar proteinsi

Entry informationi

Entry nameiSRLD_ECOLI
AccessioniPrimary (citable) accession number: P05707
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: October 1, 1996
Last modified: October 25, 2017
This is version 138 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families