Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

PTS system glucitol/sorbitol-specific EIIA component

Gene

srlB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. The enzyme II complex composed of SrlA, SrlB and SrlE is involved in glucitol/sorbitol transport. It can also use D-mannitol.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei43Tele-phosphohistidine intermediateCurated1

GO - Molecular functioni

  • kinase activity Source: EcoCyc
  • phosphotransferase activity, alcohol group as acceptor Source: UniProtKB
  • protein-N(PI)-phosphohistidine-sugar phosphotransferase activity Source: InterPro

GO - Biological processi

  • phosphoenolpyruvate-dependent sugar phosphotransferase system Source: UniProtKB

Keywordsi

Molecular functionKinase, Transferase
Biological processPhosphotransferase system, Sugar transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:GUTB-MONOMER
MetaCyc:GUTB-MONOMER

Protein family/group databases

TCDBi4.A.4.1.1 the pts glucitol (gut) family

Names & Taxonomyi

Protein namesi
Recommended name:
PTS system glucitol/sorbitol-specific EIIA component1 Publication
Alternative name(s):
EIIA-Gut1 Publication
EIII-Gut1 Publication
Glucitol/sorbitol-specific phosphotransferase enzyme IIA component1 Publication
Gene namesi
Name:srlB
Synonyms:gutB1 Publication
Ordered Locus Names:b2704, JW2673
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10970 srlB

Subcellular locationi

  • Cytoplasm 1 Publication

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001865681 – 123PTS system glucitol/sorbitol-specific EIIA componentAdd BLAST123

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei43Phosphohistidine; by HPrPROSITE-ProRule annotation1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP05706
PRIDEiP05706

Expressioni

Inductioni

Regulated by an unusual system which consists of the activator GutM and the repressor GutR in addition to the cAMP-CRP complex.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4259356, 14 interactors
IntActiP05706, 3 interactors
STRINGi316385.ECDH10B_2872

Structurei

3D structure databases

ProteinModelPortaliP05706
SMRiP05706
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 116PTS EIIA type-5PROSITE-ProRule annotationAdd BLAST114

Domaini

The EIIA domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the EIIB domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105VI0 Bacteria
COG3731 LUCA
InParanoidiP05706
KOiK02781
OMAiFCYTIDN
PhylomeDBiP05706

Family and domain databases

Gene3Di2.40.33.40, 1 hit
InterProiView protein in InterPro
IPR004716 PTS_IIA_glucitol/sorbitol-sp
IPR036665 PTS_IIA_glucitol/sorbitol_sf
IPR018454 PTS_IIA_glucitol/sorbitol_sub
PANTHERiPTHR40398 PTHR40398, 1 hit
PfamiView protein in Pfam
PF03829 PTSIIA_gutA, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD015842 PTS_IIA_glucitol/sorbitol-sp, 1 hit
SUPFAMiSSF141530 SSF141530, 1 hit
TIGRFAMsiTIGR00849 gutA, 1 hit
PROSITEiView protein in PROSITE
PS51097 PTS_EIIA_TYPE_5, 1 hit

Sequencei

Sequence statusi: Complete.

P05706-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTVIYQTTIT RIGASAIDAL SDQMLITFRE GAPADLEEYC FIHCHGELKG
60 70 80 90 100
ALHPGLQFSL GQHRYPVTAV GSVAEDNLRE LGHVTLRFDG LNEAEFPGTV
110 120
HVAGPVPDDI APGSVLKFES VKE
Length:123
Mass (Da):13,304
Last modified:November 1, 1988 - v1
Checksum:i3BB3A2AA2399EDE4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti122K → T (PubMed:9205837).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti17I → T in strain: ECOR 6, ECOR 28, ECOR 35, ECOR 37, ECOR 51, ECOR 58, ECOR 61, ECOR 66 and ECOR 69. 1
Natural varianti51A → E in strain: ECOR 58. 1
Natural varianti59S → T in strain: ECOR 28, ECOR 37, ECOR 58 and ECOR 69. 1
Natural varianti62Q → R in strain: ECOR 51. 1
Natural varianti92N → S in strain: ECOR 51, ECOR 61 and ECOR 66. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02708 Genomic DNA Translation: AAC13412.1
M93583 Genomic DNA Translation: AAA23937.1
M93585 Genomic DNA Translation: AAA23939.1
M93586 Genomic DNA Translation: AAA23946.1
M93588 Genomic DNA Translation: AAA23942.1
M93589 Genomic DNA Translation: AAA23938.1
M93590 Genomic DNA Translation: AAA23940.1
M93599 Genomic DNA Translation: AAA23941.1
M93600 Genomic DNA Translation: AAA23943.1
M93601 Genomic DNA Translation: AAA23944.1
M93602 Genomic DNA Translation: AAA23945.1
M93603 Genomic DNA Translation: AAA23947.1
U00096 Genomic DNA Translation: AAC75746.1
AP009048 Genomic DNA Translation: BAA16565.2
PIRiB26725 WQEC3S
RefSeqiNP_417184.1, NC_000913.3
WP_000216194.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75746; AAC75746; b2704
BAA16565; BAA16565; BAA16565
GeneIDi948971
KEGGiecj:JW2673
eco:b2704
PATRICifig|1411691.4.peg.4038

Similar proteinsi

Entry informationi

Entry nameiPTHA_ECOLI
AccessioniPrimary (citable) accession number: P05706
Secondary accession number(s): P77029
, Q47243, Q47244, Q57043, Q57084, Q57246
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: November 1, 1988
Last modified: March 28, 2018
This is version 149 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health