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Protein

PTS system glucitol/sorbitol-specific EIIA component

Gene

srlB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. The enzyme II complex composed of SrlA, SrlB and SrlE is involved in glucitol/sorbitol transport. It can also use D-mannitol.1 Publication

Catalytic activityi

[Protein]-N(pi)-phospho-L-histidine + D-sorbitol(Side 1) = [protein]-L-histidine + D-sorbitol 6-phosphate(Side 2).1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei43 – 431Tele-phosphohistidine intermediateCurated

GO - Molecular functioni

  • kinase activity Source: EcoCyc
  • protein-N(PI)-phosphohistidine-sugar phosphotransferase activity Source: InterPro

GO - Biological processi

  • phosphoenolpyruvate-dependent sugar phosphotransferase system Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Phosphotransferase system, Sugar transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:GUTB-MONOMER.
ECOL316407:JW2673-MONOMER.
MetaCyc:GUTB-MONOMER.

Protein family/group databases

TCDBi4.A.4.1.1. the pts glucitol (gut) family.

Names & Taxonomyi

Protein namesi
Recommended name:
PTS system glucitol/sorbitol-specific EIIA component1 Publication (EC:2.7.1.1981 Publication)
Alternative name(s):
EIIA-Gut1 Publication
EIII-Gut1 Publication
Glucitol/sorbitol-specific phosphotransferase enzyme IIA component1 Publication
Gene namesi
Name:srlB
Synonyms:gutB1 Publication
Ordered Locus Names:b2704, JW2673
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10970. srlB.

Subcellular locationi

  • Cytoplasm 1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 123123PTS system glucitol/sorbitol-specific EIIA componentPRO_0000186568Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei43 – 431Phosphohistidine; by HPrPROSITE-ProRule annotation

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP05706.

Expressioni

Inductioni

Regulated by an unusual system which consists of the activator GutM and the repressor GutR in addition to the cAMP-CRP complex.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4259356. 11 interactions.
IntActiP05706. 3 interactions.
STRINGi511145.b2704.

Structurei

3D structure databases

ProteinModelPortaliP05706.
SMRiP05706. Positions 6-88.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 116114PTS EIIA type-5PROSITE-ProRule annotationAdd
BLAST

Domaini

The EIIA domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the EIIB domain.PROSITE-ProRule annotation

Sequence similaritiesi

Contains 1 PTS EIIA type-5 domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105VI0. Bacteria.
COG3731. LUCA.
InParanoidiP05706.
KOiK02781.
OMAiYQTTITH.
OrthoDBiEOG6038ZZ.
PhylomeDBiP05706.

Family and domain databases

Gene3Di2.40.33.40. 1 hit.
InterProiIPR004716. PTS_IIA_glucitol/sorbitol-sp.
IPR018454. PTS_IIA_glucitol/sorbitol_sub.
[Graphical view]
PfamiPF03829. PTSIIA_gutA. 1 hit.
[Graphical view]
ProDomiPD015842. PTS_IIA_glucitol/sorbitol-sp. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF141530. SSF141530. 1 hit.
TIGRFAMsiTIGR00849. gutA. 1 hit.
PROSITEiPS51097. PTS_EIIA_TYPE_5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P05706-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTVIYQTTIT RIGASAIDAL SDQMLITFRE GAPADLEEYC FIHCHGELKG
60 70 80 90 100
ALHPGLQFSL GQHRYPVTAV GSVAEDNLRE LGHVTLRFDG LNEAEFPGTV
110 120
HVAGPVPDDI APGSVLKFES VKE
Length:123
Mass (Da):13,304
Last modified:November 1, 1988 - v1
Checksum:i3BB3A2AA2399EDE4
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti122 – 1221K → T (PubMed:9205837).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti17 – 171I → T in strain: ECOR 6, ECOR 28, ECOR 35, ECOR 37, ECOR 51, ECOR 58, ECOR 61, ECOR 66 and ECOR 69.
Natural varianti51 – 511A → E in strain: ECOR 58.
Natural varianti59 – 591S → T in strain: ECOR 28, ECOR 37, ECOR 58 and ECOR 69.
Natural varianti62 – 621Q → R in strain: ECOR 51.
Natural varianti92 – 921N → S in strain: ECOR 51, ECOR 61 and ECOR 66.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02708 Genomic DNA. Translation: AAC13412.1.
M93583 Genomic DNA. Translation: AAA23937.1.
M93585 Genomic DNA. Translation: AAA23939.1.
M93586 Genomic DNA. Translation: AAA23946.1.
M93588 Genomic DNA. Translation: AAA23942.1.
M93589 Genomic DNA. Translation: AAA23938.1.
M93590 Genomic DNA. Translation: AAA23940.1.
M93599 Genomic DNA. Translation: AAA23941.1.
M93600 Genomic DNA. Translation: AAA23943.1.
M93601 Genomic DNA. Translation: AAA23944.1.
M93602 Genomic DNA. Translation: AAA23945.1.
M93603 Genomic DNA. Translation: AAA23947.1.
U00096 Genomic DNA. Translation: AAC75746.1.
AP009048 Genomic DNA. Translation: BAA16565.2.
PIRiB26725. WQEC3S.
RefSeqiNP_417184.1. NC_000913.3.
WP_000216194.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75746; AAC75746; b2704.
BAA16565; BAA16565; BAA16565.
GeneIDi948971.
KEGGiecj:JW2673.
eco:b2704.
PATRICi32120806. VBIEscCol129921_2795.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02708 Genomic DNA. Translation: AAC13412.1.
M93583 Genomic DNA. Translation: AAA23937.1.
M93585 Genomic DNA. Translation: AAA23939.1.
M93586 Genomic DNA. Translation: AAA23946.1.
M93588 Genomic DNA. Translation: AAA23942.1.
M93589 Genomic DNA. Translation: AAA23938.1.
M93590 Genomic DNA. Translation: AAA23940.1.
M93599 Genomic DNA. Translation: AAA23941.1.
M93600 Genomic DNA. Translation: AAA23943.1.
M93601 Genomic DNA. Translation: AAA23944.1.
M93602 Genomic DNA. Translation: AAA23945.1.
M93603 Genomic DNA. Translation: AAA23947.1.
U00096 Genomic DNA. Translation: AAC75746.1.
AP009048 Genomic DNA. Translation: BAA16565.2.
PIRiB26725. WQEC3S.
RefSeqiNP_417184.1. NC_000913.3.
WP_000216194.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP05706.
SMRiP05706. Positions 6-88.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259356. 11 interactions.
IntActiP05706. 3 interactions.
STRINGi511145.b2704.

Protein family/group databases

TCDBi4.A.4.1.1. the pts glucitol (gut) family.

Proteomic databases

PaxDbiP05706.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75746; AAC75746; b2704.
BAA16565; BAA16565; BAA16565.
GeneIDi948971.
KEGGiecj:JW2673.
eco:b2704.
PATRICi32120806. VBIEscCol129921_2795.

Organism-specific databases

EchoBASEiEB0963.
EcoGeneiEG10970. srlB.

Phylogenomic databases

eggNOGiENOG4105VI0. Bacteria.
COG3731. LUCA.
InParanoidiP05706.
KOiK02781.
OMAiYQTTITH.
OrthoDBiEOG6038ZZ.
PhylomeDBiP05706.

Enzyme and pathway databases

BioCyciEcoCyc:GUTB-MONOMER.
ECOL316407:JW2673-MONOMER.
MetaCyc:GUTB-MONOMER.

Miscellaneous databases

PROiP05706.

Family and domain databases

Gene3Di2.40.33.40. 1 hit.
InterProiIPR004716. PTS_IIA_glucitol/sorbitol-sp.
IPR018454. PTS_IIA_glucitol/sorbitol_sub.
[Graphical view]
PfamiPF03829. PTSIIA_gutA. 1 hit.
[Graphical view]
ProDomiPD015842. PTS_IIA_glucitol/sorbitol-sp. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF141530. SSF141530. 1 hit.
TIGRFAMsiTIGR00849. gutA. 1 hit.
PROSITEiPS51097. PTS_EIIA_TYPE_5. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Glucitol-specific enzymes of the phosphotransferase system in Escherichia coli. Nucleotide sequence of the gut operon."
    Yamada M., Saier M.H. Jr.
    J. Biol. Chem. 262:5455-5463(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION.
  2. "Molecular population genetics of Escherichia coli: DNA sequence diversity at the celC, crr, and gutB loci of natural isolates."
    Hall B.G., Sharp P.M.
    Mol. Biol. Evol. 9:654-665(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Various ECOR strains.
  3. "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features."
    Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T.
    , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.
    DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION TO 122.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "Nature and properties of hexitol transport systems in Escherichia coli."
    Lengeler J.
    J. Bacteriol. 124:39-47(1975) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, SUBSTRATE SPECIFICITY.
  7. "Positive and negative regulators for glucitol (gut) operon expression in Escherichia coli."
    Yamada M., Saier M.H. Jr.
    J. Mol. Biol. 203:569-583(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.

Entry informationi

Entry nameiPTHA_ECOLI
AccessioniPrimary (citable) accession number: P05706
Secondary accession number(s): P77029
, Q47243, Q47244, Q57043, Q57084, Q57246
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: November 1, 1988
Last modified: July 6, 2016
This is version 138 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.