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Protein

Methyl-accepting chemotaxis protein III

Gene

trg

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mediates taxis to the sugars ribose and galactose via an interaction with the periplasmic ribose- or galactose-binding proteins.
Chemotactic-signal transducers respond to changes in the concentration of attractants and repellents in the environment, transduce a signal from the outside to the inside of the cell, and facilitate sensory adaptation through the variation of the level of methylation. Attractants increase the level of methylation while repellents decrease the level of methylation, the methyl groups are added by the methyltransferase CheR and removed by the methylesterase CheB.

GO - Molecular functioni

  • protein homodimerization activity Source: CAFA
  • transmembrane signaling receptor activity Source: EcoCyc

GO - Biological processi

  • chemotaxis Source: EcoCyc
  • signal transduction Source: EcoCyc

Keywordsi

Molecular functionTransducer
Biological processChemotaxis

Enzyme and pathway databases

BioCyciEcoCyc:TRG-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Methyl-accepting chemotaxis protein III
Short name:
MCP-III
Alternative name(s):
Ribose and galactose chemoreceptor protein
Gene namesi
Name:trg
Ordered Locus Names:b1421, JW1417
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11018. trg.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 23CytoplasmicSequence analysisAdd BLAST23
Transmembranei24 – 44HelicalSequence analysisAdd BLAST21
Topological domaini45 – 201PeriplasmicSequence analysisAdd BLAST157
Transmembranei202 – 222HelicalSequence analysisAdd BLAST21
Topological domaini223 – 546CytoplasmicSequence analysisAdd BLAST324

GO - Cellular componenti

  • integral component of plasma membrane Source: CAFA
  • methyl accepting chemotaxis protein complex Source: CAFA
  • plasma membrane Source: EcoCyc

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001105401 – 546Methyl-accepting chemotaxis protein IIIAdd BLAST546

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei305Glutamate methyl ester (Glu)2 Publications1
Modified residuei312Glutamate methyl ester (Gln)2 Publications1
Modified residuei319Glutamate methyl ester (Gln)2 Publications1
Modified residuei501Glutamate methyl ester (Glu)2 Publications1
Modified residuei510Glutamate methyl ester (Glu)1 Publication1

Keywords - PTMi

Methylation

Proteomic databases

PaxDbiP05704.
PRIDEiP05704.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
frlDP455435EBI-557436,EBI-562037

GO - Molecular functioni

  • protein homodimerization activity Source: CAFA

Protein-protein interaction databases

BioGridi4261384. 311 interactors.
DIPiDIP-11027N.
IntActiP05704. 5 interactors.
MINTiMINT-1228342.
STRINGi316385.ECDH10B_1549.

Structurei

3D structure databases

ProteinModelPortaliP05704.
SMRiP05704.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini224 – 276HAMPPROSITE-ProRule annotationAdd BLAST53
Domaini281 – 510Methyl-accepting transducerPROSITE-ProRule annotationAdd BLAST230

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105C8Q. Bacteria.
COG0840. LUCA.
HOGENOMiHOG000148074.
InParanoidiP05704.
KOiK05876.
PhylomeDBiP05704.

Family and domain databases

CDDicd06225. HAMP. 1 hit.
cd00181. Tar_Tsr_LBD. 1 hit.
Gene3Di1.20.120.30. 1 hit.
InterProiView protein in InterPro
IPR004090. Chemotax_Me-accpt_rcpt.
IPR004091. Chemotax_Me-accpt_rcpt_Me-site.
IPR035440. Four_Helix_Bundle_domain.
IPR003660. HAMP_dom.
IPR004089. MCPsignal_dom.
IPR003122. Tar_rcpt_lig-bd.
PfamiView protein in Pfam
PF00672. HAMP. 1 hit.
PF00015. MCPsignal. 1 hit.
PF02203. TarH. 1 hit.
PRINTSiPR00260. CHEMTRNSDUCR.
SMARTiView protein in SMART
SM00304. HAMP. 1 hit.
SM00283. MA. 1 hit.
SM00319. TarH. 1 hit.
SUPFAMiSSF47170. SSF47170. 1 hit.
PROSITEiView protein in PROSITE
PS00538. CHEMOTAXIS_TRANSDUC_1. 1 hit.
PS50111. CHEMOTAXIS_TRANSDUC_2. 1 hit.
PS50885. HAMP. 1 hit.

Sequencei

Sequence statusi: Complete.

P05704-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNTTPSQRLG FLHHIRLVPL FACILGGILV LFALSSALAG YFLWQADRDQ
60 70 80 90 100
RDVTAEIEIR TGLANSSDFL RSARINMIQA GAASRIAEME AMKRNIAQAE
110 120 130 140 150
SEIKQSQQGY RAYQNRPVKT PADEALDTEL NQRFQAYITG MQPMLKYAKN
160 170 180 190 200
GMFEAIINHE SEQIRPLDNA YTDILNKAVK IRSTRANQLA ELAHQRTRLG
210 220 230 240 250
GMFMIGAFVL ALVMTLITFM VLRRIVIRPL QHAAQRIEKI ASGDLTMNDE
260 270 280 290 300
PAGRNEIGRL SRHLQQMQHS LGMTVGTVRQ GAEEIYRGTS EISAGNADLS
310 320 330 340 350
SRTEEQAAAI EQTAASMEQL TATVKQNADN AHHASKLAQE ASIKASDGGQ
360 370 380 390 400
TVSGVVKTMG AISTSSKKIS EITAVINSIA FQTNILALNA AVEAARAGEQ
410 420 430 440 450
GRGFAVVASE VRTLASRSAQ AAKEIEGLIS ESVRLIDLGS DEVATAGKTM
460 470 480 490 500
STIVDAVASV THIMQEIAAA SDEQSRGITQ VSQAISEMDK VTQQNASLVE
510 520 530 540
EASAAAVSLE EQAARLTEAV DVFRLHKHSV SAEPRGAGEP VSFATV
Length:546
Mass (Da):58,899
Last modified:November 1, 1997 - v3
Checksum:iFDB40374C0E83E7B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K02073 Genomic DNA. Translation: AAA81329.2.
U00096 Genomic DNA. Translation: AAC74503.1.
AP009048 Genomic DNA. Translation: BAA15044.1.
PIRiH64893. QREC3M.
RefSeqiNP_415938.1. NC_000913.3.
WP_001098559.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74503; AAC74503; b1421.
BAA15044; BAA15044; BAA15044.
GeneIDi945995.
KEGGiecj:JW1417.
eco:b1421.
PATRICifig|1411691.4.peg.849.

Similar proteinsi

Entry informationi

Entry nameiMCP3_ECOLI
AccessioniPrimary (citable) accession number: P05704
Secondary accession number(s): P77448
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: November 1, 1997
Last modified: August 30, 2017
This is version 154 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene