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Protein

Porin P

Gene

oprP

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Anion specific, the binding site has higher affinity for phosphate than chloride ions. Porin O has a higher affinity for polyphosphates (tripolyphosphate and pyrophosphate) while porin P has a higher affinity for orthophosphate.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Porin

Keywords - Biological processi

Ion transport, Transport

Keywords - Ligandi

Chloride

Protein family/group databases

TCDBi1.B.5.1.1. the pseudomonas oprp porin (pop) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Porin P
Alternative name(s):
Outer membrane protein D1
Gene namesi
Name:oprP
Ordered Locus Names:PA3279
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA3279.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell outer membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 29291 PublicationAdd
BLAST
Chaini30 – 440411Porin PPRO_0000025212Add
BLAST

Proteomic databases

PaxDbiP05695.

Interactioni

Subunit structurei

Homotrimer. Trimer pore size is 0.6 nM.

Protein-protein interaction databases

DIPiDIP-29262N.
STRINGi208964.PA3279.

Structurei

Secondary structure

1
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi31 – 333Combined sources
Beta strandi35 – 373Combined sources
Beta strandi39 – 424Combined sources
Beta strandi44 – 518Combined sources
Beta strandi57 – 7317Combined sources
Beta strandi76 – 805Combined sources
Beta strandi82 – 9817Combined sources
Turni99 – 1013Combined sources
Beta strandi102 – 1098Combined sources
Beta strandi114 – 1163Combined sources
Turni117 – 1193Combined sources
Beta strandi122 – 1298Combined sources
Turni131 – 1344Combined sources
Beta strandi135 – 1417Combined sources
Helixi148 – 1514Combined sources
Turni154 – 1563Combined sources
Beta strandi158 – 1614Combined sources
Helixi164 – 1685Combined sources
Helixi170 – 1734Combined sources
Beta strandi176 – 18611Combined sources
Turni187 – 1893Combined sources
Beta strandi190 – 20314Combined sources
Beta strandi208 – 22316Combined sources
Beta strandi226 – 23813Combined sources
Helixi255 – 2573Combined sources
Beta strandi260 – 2623Combined sources
Helixi266 – 2683Combined sources
Beta strandi283 – 29816Combined sources
Beta strandi301 – 31414Combined sources
Beta strandi321 – 33313Combined sources
Turni343 – 3464Combined sources
Turni356 – 3583Combined sources
Beta strandi360 – 37415Combined sources
Beta strandi382 – 3843Combined sources
Beta strandi389 – 40113Combined sources
Beta strandi403 – 42018Combined sources
Beta strandi428 – 44013Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2O4VX-ray1.94A/B/C30-440[»]
ProteinModelPortaliP05695.
SMRiP05695. Positions 30-440.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP05695.

Family & Domainsi

Sequence similaritiesi

Belongs to the OprO/OprP family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane beta strand

Phylogenomic databases

eggNOGiENOG4108SDF. Bacteria.
COG3746. LUCA.
HOGENOMiHOG000247361.
InParanoidiP05695.
KOiK07221.
OMAiFRWGVSA.
OrthoDBiEOG65J4ZC.

Family and domain databases

Gene3Di2.40.160.10. 1 hit.
InterProiIPR023614. Porin_dom.
IPR010870. Porin_O/P.
[Graphical view]
PfamiPF07396. Porin_O_P. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P05695-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIRRHSCKGV GSSVAWSLLG LAISAQSLAG TVTTDGADIV IKTKGGLEVA
60 70 80 90 100
TTDKEFSFKL GGRLQADYGR FDGYYTNNGN TADAAYFRRA YLEFGGTAYR
110 120 130 140 150
DWKYQINYDL SRNVGNDSAG YFDEASVTYT GFNPVNLKFG RFYTDFGLEK
160 170 180 190 200
ATSSKWVTAL ERNLTYDIAD WVNDNVGTGI QASSVVGGMA FLSGSVFSEN
210 220 230 240 250
NNDTDGDSVK RYNLRGVFAP LHEPGNVVHL GLQYAYRDLE DSAVDTRIRP
260 270 280 290 300
RMGMRGVSTN GGNDAGSNGN RGLFGGSSAV EGLWKDDSVW GLEGAWALGA
310 320 330 340 350
FSAQAEYLRR TVKAERDRED LKASGYYAQL AYTLTGEPRL YKLDGAKFDT
360 370 380 390 400
IKPENKEIGA WELFYRYDSI KVEDDNIVVD SATREVGDAK GKTHTLGVNW
410 420 430 440
YANEAVKVSA NYVKAKTDKI SNANGDDSGD GLVMRLQYVF
Length:440
Mass (Da):48,211
Last modified:December 8, 2000 - v3
Checksum:i840243D11CDC7C26
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti208 – 2081S → T in CAA37395 (PubMed:1697017).Curated
Sequence conflicti208 – 2081S → T in AAA25913 (PubMed:1697017).Curated
Sequence conflicti394 – 3952HT → RA in CAA37395 (PubMed:1697017).Curated
Sequence conflicti394 – 3952HT → RA in AAA25913 (PubMed:1697017).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53313 Genomic DNA. Translation: CAA37395.1.
M86648 Genomic DNA. Translation: AAA25913.1.
AE004091 Genomic DNA. Translation: AAG06667.1.
Y00553 Genomic DNA. Translation: CAA68631.1.
PIRiF83235.
S11793.
RefSeqiNP_251969.1. NC_002516.2.
WP_003119678.1. NZ_ASJY01000524.1.

Genome annotation databases

EnsemblBacteriaiAAG06667; AAG06667; PA3279.
GeneIDi882442.
KEGGipae:PA3279.
PATRICi19841139. VBIPseAer58763_3428.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53313 Genomic DNA. Translation: CAA37395.1.
M86648 Genomic DNA. Translation: AAA25913.1.
AE004091 Genomic DNA. Translation: AAG06667.1.
Y00553 Genomic DNA. Translation: CAA68631.1.
PIRiF83235.
S11793.
RefSeqiNP_251969.1. NC_002516.2.
WP_003119678.1. NZ_ASJY01000524.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2O4VX-ray1.94A/B/C30-440[»]
ProteinModelPortaliP05695.
SMRiP05695. Positions 30-440.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-29262N.
STRINGi208964.PA3279.

Protein family/group databases

TCDBi1.B.5.1.1. the pseudomonas oprp porin (pop) family.

Proteomic databases

PaxDbiP05695.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG06667; AAG06667; PA3279.
GeneIDi882442.
KEGGipae:PA3279.
PATRICi19841139. VBIPseAer58763_3428.

Organism-specific databases

PseudoCAPiPA3279.

Phylogenomic databases

eggNOGiENOG4108SDF. Bacteria.
COG3746. LUCA.
HOGENOMiHOG000247361.
InParanoidiP05695.
KOiK07221.
OMAiFRWGVSA.
OrthoDBiEOG65J4ZC.

Miscellaneous databases

EvolutionaryTraceiP05695.

Family and domain databases

Gene3Di2.40.160.10. 1 hit.
InterProiIPR023614. Porin_dom.
IPR010870. Porin_O/P.
[Graphical view]
PfamiPF07396. Porin_O_P. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and relatedness in other bacteria of the Pseudomonas aeruginosa oprP gene coding for the phosphate-specific porin P."
    Siehnel R.J., Martin N.L., Hancock R.E.W.
    Mol. Microbiol. 4:831-838(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 15692 / PAO1 / H103.
  2. "Polyphosphate-selective porin OprO of Pseudomonas aeruginosa: expression, purification and sequence."
    Siehnel R.J., Egli C., Hancock R.E.W.
    Mol. Microbiol. 6:2319-2326(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 15692 / PAO1 / H103.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
  4. "Regulation of components of the Pseudomonas aeruginosa phosphate-starvation-inducible regulon in Escherichia coli."
    Siehnel R.J., Worobec E.A., Hancock R.E.W.
    Mol. Microbiol. 2:347-352(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-56.
  5. "Large-scale purification and biochemical characterization of crystallization-grade porin protein P from Pseudomonas aeruginosa."
    Worobec E.A., Martin N.L., McCubbin W.D., Kay C.M., Brayer G.D., Hancock R.E.W.
    Biochim. Biophys. Acta 939:366-374(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 30-65.
  6. "Demonstration and chemical modification of a specific phosphate binding site in the phosphate-starvation-inducible outer membrane porin protein P of Pseudomonas aeruginosa."
    Hancock R.E.W., Benz R.
    Biochim. Biophys. Acta 860:699-707(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.

Entry informationi

Entry nameiPORP_PSEAE
AccessioniPrimary (citable) accession number: P05695
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: December 8, 2000
Last modified: November 11, 2015
This is version 109 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.