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Protein

5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase

Gene

MET6

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation.

Miscellaneous

Present with 264000 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine.

Cofactori

Zn2+By similarity

Pathwayi: L-methionine biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes L-methionine from L-homocysteine (MetE route).
Proteins known to be involved in this subpathway in this organism are:
  1. 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (MET6)
This subpathway is part of the pathway L-methionine biosynthesis via de novo pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-methionine from L-homocysteine (MetE route), the pathway L-methionine biosynthesis via de novo pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi655ZincBy similarity1
Metal bindingi657ZincBy similarity1
Metal bindingi737ZincBy similarity1

GO - Molecular functioni

GO - Biological processi

  • homocysteine metabolic process Source: GO_Central
  • methionine biosynthetic process Source: SGD

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processAmino-acid biosynthesis, Methionine biosynthesis
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciMetaCyc:YER091C-MONOMER
YEAST:YER091C-MONOMER
BRENDAi2.1.1.14 984
UniPathwayiUPA00051; UER00082

Names & Taxonomyi

Protein namesi
Recommended name:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (EC:2.1.1.14)
Alternative name(s):
Cobalamin-independent methionine synthase
Delta-P8 protein
Methionine synthase, vitamin-B12 independent isozyme
Gene namesi
Name:MET6
Ordered Locus Names:YER091C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YER091C
SGDiS000000893 MET6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000987042 – 7675-methyltetrahydropteroyltriglutamate--homocysteine methyltransferaseAdd BLAST766

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei89PhosphoserineCombined sources1
Modified residuei242PhosphoserineCombined sources1
Modified residuei566PhosphothreonineCombined sources1
Modified residuei629PhosphoserineCombined sources1
Modified residuei706PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP05694
PaxDbiP05694
PRIDEiP05694
TopDownProteomicsiP05694

2D gel databases

SWISS-2DPAGEiP05694

PTM databases

CarbonylDBiP05694
iPTMnetiP05694

Interactioni

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi36836, 142 interactors
DIPiDIP-6472N
IntActiP05694, 57 interactors
MINTiP05694
STRINGi4932.YER091C

Structurei

3D structure databases

ProteinModelPortaliP05694
SMRiP05694
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000246221
InParanoidiP05694
KOiK00549
OMAiGRNIWKN
OrthoDBiEOG092C0LN1

Family and domain databases

Gene3Di3.20.20.210, 4 hits
HAMAPiMF_00172 Meth_synth, 1 hit
InterProiView protein in InterPro
IPR013215 Cbl-indep_Met_Synth_N
IPR006276 Cobalamin-indep_Met_synthase
IPR002629 Met_Synth_C/arc
IPR038071 UROD/MetE-like_sf
PfamiView protein in Pfam
PF08267 Meth_synt_1, 1 hit
PF01717 Meth_synt_2, 1 hit
PIRSFiPIRSF000382 MeTrfase_B12_ind, 1 hit
SUPFAMiSSF51726 SSF51726, 2 hits
TIGRFAMsiTIGR01371 met_syn_B12ind, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P05694-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVQSAVLGFP RIGPNRELKK ATEGYWNGKI TVDELFKVGK DLRTQNWKLQ
60 70 80 90 100
KEAGVDIIPS NDFSFYDQVL DLSLLFNVIP DRYTKYDLSP IDTLFAMGRG
110 120 130 140 150
LQRKATETEK AVDVTALEMV KWFDSNYHYV RPTFSKTTQF KLNGQKPVDE
160 170 180 190 200
FLEAKELGIH TRPVLLGPVS YLFLGKADKD SLDLEPLSLL EQLLPLYTEI
210 220 230 240 250
LSKLASAGAT EVQIDEPVLV LDLPANAQAA IKKAYTYFGE QSNLPKITLA
260 270 280 290 300
TYFGTVVPNL DAIKGLPVAA LHVDFVRAPE QFDEVVAAIG NKQTLSVGIV
310 320 330 340 350
DGRNIWKNDF KKSSAIVNKA IEKLGADRVV VATSSSLLHT PVDLNNETKL
360 370 380 390 400
DAEIKGFFSF ATQKLDEVVV ITKNVSGQDV AAALEANAKS VESRGKSKFI
410 420 430 440 450
HDAAVKARVA SIDEKMSTRA APFEQRLPEQ QKVFNLPLFP TTTIGSFPQT
460 470 480 490 500
KDIRINRNKF NKGTISAEEY EKFINSEIEK VIRFQEEIGL DVLVHGEPER
510 520 530 540 550
NDMVQYFGEQ INGYAFTVNG WVQSYGSRYV RPPIIVGDLS RPKAMSVKES
560 570 580 590 600
VYAQSITSKP VKGMLTGPIT CLRWSFPRDD VDQKTQAMQL ALALRDEVND
610 620 630 640 650
LEAAGIKVIQ VDEPALREGL PLREGTERSA YYTWAAEAFR VATSGVANKT
660 670 680 690 700
QIHSHFCYSD LDPNHIKALD ADVVSIEFSK KDDANYIAEF KNYPNHIGLG
710 720 730 740 750
LFDIHSPRIP SKDEFIAKIS TILKSYPAEK FWVNPDCGLK TRGWEETRLS
760
LTHMVEAAKY FREQYKN
Length:767
Mass (Da):85,860
Last modified:January 23, 2007 - v4
Checksum:iA553C9A98D35541D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti72 – 80LSLLFNVIP → FVFVVQCHS in CAA30227 (Ref. 6) Curated9
Sequence conflicti407A → R in AAA65711 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U32508 Genomic DNA Translation: AAB60301.1
U15099 Genomic DNA Translation: AAA65711.1
DQ393811 Genomic DNA Translation: ABD57965.1
DQ393812 Genomic DNA Translation: ABD57966.1
U18839 Genomic DNA Translation: AAB64646.1
X07238 Genomic DNA Translation: CAA30227.1
BK006939 Genomic DNA Translation: DAA07752.1
PIRiS50594
RefSeqiNP_011015.3, NM_001178982.3

Genome annotation databases

EnsemblFungiiYER091C; YER091C; YER091C
GeneIDi856825
KEGGisce:YER091C

Similar proteinsi

Entry informationi

Entry nameiMETE_YEAST
AccessioniPrimary (citable) accession number: P05694
Secondary accession number(s): D3DLZ8
, P38010, Q02488, Q27JJ8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: January 23, 2007
Last modified: March 28, 2018
This is version 176 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health