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Protein

5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase

Gene

MET6

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation.

Catalytic activityi

5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine.

Cofactori

Zn2+By similarity

Pathwayi: L-methionine biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes L-methionine from L-homocysteine (MetE route).
Proteins known to be involved in this subpathway in this organism are:
  1. 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (MET6)
This subpathway is part of the pathway L-methionine biosynthesis via de novo pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-methionine from L-homocysteine (MetE route), the pathway L-methionine biosynthesis via de novo pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi655 – 6551ZincBy similarity
Metal bindingi657 – 6571ZincBy similarity
Metal bindingi737 – 7371ZincBy similarity

GO - Molecular functioni

  • 5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Source: SGD
  • zinc ion binding Source: InterPro

GO - Biological processi

  • methionine biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Methionine biosynthesis

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-403.
YEAST:YER091C-MONOMER.
BRENDAi2.1.1.14. 984.
UniPathwayiUPA00051; UER00082.

Names & Taxonomyi

Protein namesi
Recommended name:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (EC:2.1.1.14)
Alternative name(s):
Cobalamin-independent methionine synthase
Delta-P8 protein
Methionine synthase, vitamin-B12 independent isozyme
Gene namesi
Name:MET6
Ordered Locus Names:YER091C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YER091C.
SGDiS000000893. MET6.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 7677665-methyltetrahydropteroyltriglutamate--homocysteine methyltransferasePRO_0000098704Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei89 – 891PhosphoserineCombined sources
Modified residuei242 – 2421PhosphoserineCombined sources
Modified residuei566 – 5661PhosphothreonineCombined sources
Modified residuei629 – 6291PhosphoserineCombined sources
Modified residuei706 – 7061PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP05694.
PeptideAtlasiP05694.
PRIDEiP05694.
TopDownProteomicsiP05694.

2D gel databases

SWISS-2DPAGEP05694.

PTM databases

iPTMnetiP05694.

Interactioni

Protein-protein interaction databases

BioGridi36836. 89 interactions.
DIPiDIP-6472N.
IntActiP05694. 6 interactions.
MINTiMINT-677379.

Structurei

3D structure databases

ProteinModelPortaliP05694.
SMRiP05694. Positions 1-765.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000246221.
InParanoidiP05694.
KOiK00549.
OMAiDDANYIA.
OrthoDBiEOG7BGHV7.

Family and domain databases

HAMAPiMF_00172. Meth_synth.
InterProiIPR013215. Cbl-indep_Met_Synth_N.
IPR006276. Cobalamin-indep_Met_synthase.
IPR002629. Met_Synth_C/arc.
[Graphical view]
PfamiPF08267. Meth_synt_1. 1 hit.
PF01717. Meth_synt_2. 1 hit.
[Graphical view]
PIRSFiPIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMsiTIGR01371. met_syn_B12ind. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P05694-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVQSAVLGFP RIGPNRELKK ATEGYWNGKI TVDELFKVGK DLRTQNWKLQ
60 70 80 90 100
KEAGVDIIPS NDFSFYDQVL DLSLLFNVIP DRYTKYDLSP IDTLFAMGRG
110 120 130 140 150
LQRKATETEK AVDVTALEMV KWFDSNYHYV RPTFSKTTQF KLNGQKPVDE
160 170 180 190 200
FLEAKELGIH TRPVLLGPVS YLFLGKADKD SLDLEPLSLL EQLLPLYTEI
210 220 230 240 250
LSKLASAGAT EVQIDEPVLV LDLPANAQAA IKKAYTYFGE QSNLPKITLA
260 270 280 290 300
TYFGTVVPNL DAIKGLPVAA LHVDFVRAPE QFDEVVAAIG NKQTLSVGIV
310 320 330 340 350
DGRNIWKNDF KKSSAIVNKA IEKLGADRVV VATSSSLLHT PVDLNNETKL
360 370 380 390 400
DAEIKGFFSF ATQKLDEVVV ITKNVSGQDV AAALEANAKS VESRGKSKFI
410 420 430 440 450
HDAAVKARVA SIDEKMSTRA APFEQRLPEQ QKVFNLPLFP TTTIGSFPQT
460 470 480 490 500
KDIRINRNKF NKGTISAEEY EKFINSEIEK VIRFQEEIGL DVLVHGEPER
510 520 530 540 550
NDMVQYFGEQ INGYAFTVNG WVQSYGSRYV RPPIIVGDLS RPKAMSVKES
560 570 580 590 600
VYAQSITSKP VKGMLTGPIT CLRWSFPRDD VDQKTQAMQL ALALRDEVND
610 620 630 640 650
LEAAGIKVIQ VDEPALREGL PLREGTERSA YYTWAAEAFR VATSGVANKT
660 670 680 690 700
QIHSHFCYSD LDPNHIKALD ADVVSIEFSK KDDANYIAEF KNYPNHIGLG
710 720 730 740 750
LFDIHSPRIP SKDEFIAKIS TILKSYPAEK FWVNPDCGLK TRGWEETRLS
760
LTHMVEAAKY FREQYKN
Length:767
Mass (Da):85,860
Last modified:January 23, 2007 - v4
Checksum:iA553C9A98D35541D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti72 – 809LSLLFNVIP → FVFVVQCHS in CAA30227 (Ref. 6) Curated
Sequence conflicti407 – 4071A → R in AAA65711 (Ref. 2) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U32508 Genomic DNA. Translation: AAB60301.1.
U15099 Genomic DNA. Translation: AAA65711.1.
DQ393811 Genomic DNA. Translation: ABD57965.1.
DQ393812 Genomic DNA. Translation: ABD57966.1.
U18839 Genomic DNA. Translation: AAB64646.1.
X07238 Genomic DNA. Translation: CAA30227.1.
BK006939 Genomic DNA. Translation: DAA07752.1.
PIRiS50594.
RefSeqiNP_011015.3. NM_001178982.3.

Genome annotation databases

EnsemblFungiiYER091C; YER091C; YER091C.
GeneIDi856825.
KEGGisce:YER091C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U32508 Genomic DNA. Translation: AAB60301.1.
U15099 Genomic DNA. Translation: AAA65711.1.
DQ393811 Genomic DNA. Translation: ABD57965.1.
DQ393812 Genomic DNA. Translation: ABD57966.1.
U18839 Genomic DNA. Translation: AAB64646.1.
X07238 Genomic DNA. Translation: CAA30227.1.
BK006939 Genomic DNA. Translation: DAA07752.1.
PIRiS50594.
RefSeqiNP_011015.3. NM_001178982.3.

3D structure databases

ProteinModelPortaliP05694.
SMRiP05694. Positions 1-765.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36836. 89 interactions.
DIPiDIP-6472N.
IntActiP05694. 6 interactions.
MINTiMINT-677379.

PTM databases

iPTMnetiP05694.

2D gel databases

SWISS-2DPAGEP05694.

Proteomic databases

MaxQBiP05694.
PeptideAtlasiP05694.
PRIDEiP05694.
TopDownProteomicsiP05694.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYER091C; YER091C; YER091C.
GeneIDi856825.
KEGGisce:YER091C.

Organism-specific databases

EuPathDBiFungiDB:YER091C.
SGDiS000000893. MET6.

Phylogenomic databases

HOGENOMiHOG000246221.
InParanoidiP05694.
KOiK00549.
OMAiDDANYIA.
OrthoDBiEOG7BGHV7.

Enzyme and pathway databases

UniPathwayiUPA00051; UER00082.
BioCyciMetaCyc:MONOMER-403.
YEAST:YER091C-MONOMER.
BRENDAi2.1.1.14. 984.

Miscellaneous databases

NextBioi983115.
PROiP05694.

Family and domain databases

HAMAPiMF_00172. Meth_synth.
InterProiIPR013215. Cbl-indep_Met_Synth_N.
IPR006276. Cobalamin-indep_Met_synthase.
IPR002629. Met_Synth_C/arc.
[Graphical view]
PfamiPF08267. Meth_synt_1. 1 hit.
PF01717. Meth_synt_2. 1 hit.
[Graphical view]
PIRSFiPIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMsiTIGR01371. met_syn_B12ind. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structure and regulation of MET6, the vitamin B12-independent methionine synthase gene of Saccharomyces cerevisiae."
    Korch C., Mountain H.A., Wenzlau J.M.
    Submitted (JUL-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "Cloning and characterization of MET6: a cobalamin-independent methionine synthase in Saccharomyces cerevisiae."
    McClurg J.C.
    Thesis (1995), Medical College of Ohio / Toledo, United States
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Allele diversity among genes of the sulfate reduction pathway in wine strains of Saccharomyces cerevisiae."
    Linderholm A.L., Bisson L.F.
    Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: UCD939 and UCD940.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  6. "The expression of the gamma-glutamylcysteine synthetase gene of Escherichia coli B in Saccharomyces cerevisiae."
    Ohtake Y., Watanabe K., Tezuka H., Ogata T., Yabuuchi S., Murata K., Kimura A.
    Agric. Biol. Chem. 52:2753-2762(1988)
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-486.
    Strain: YMN27.
  7. "Two-dimensional protein map of Saccharomyces cerevisiae: construction of a gene-protein index."
    Boucherie H., Dujardin G., Kermorgant M., Monribot C., Slonimski P.P., Perrot M.
    Yeast 11:601-613(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-14.
    Strain: ATCC 204508 / S288c.
  8. Cited for: PROTEIN SEQUENCE OF 693-704.
    Strain: ATCC 204508 / S288c.
  9. "Protein expression during exponential growth in 0.7 M NaCl medium of Saccharomyces cerevisiae."
    Norbeck J., Blomberg A.
    FEMS Microbiol. Lett. 137:1-8(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 123-132; 234-243 AND 692-699.
    Strain: ATCC 38531 / Y41.
  10. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  11. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-89 AND SER-706, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  12. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-242, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  13. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-566; SER-629 AND SER-706, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  14. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMETE_YEAST
AccessioniPrimary (citable) accession number: P05694
Secondary accession number(s): D3DLZ8
, P38010, Q02488, Q27JJ8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: January 23, 2007
Last modified: May 11, 2016
This is version 159 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 264000 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.