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P05655

- SACB_BACSU

UniProt

P05655 - SACB_BACSU

Protein

Levansucrase

Gene

sacB

Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 1 (01 Nov 1988)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Sucrose + (6)-beta-D-fructofuranosyl-(2->)(n) alpha-D-glucopyranoside = glucose + (6)-beta-D-fructofuranosyl-(2->)(n+1) alpha-D-glucopyranoside.

    GO - Molecular functioni

    1. levansucrase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate utilization Source: InterPro

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Enzyme and pathway databases

    BioCyciBSUB:BSU34450-MONOMER.
    MetaCyc:BSU34450-MONOMER.
    SABIO-RKP05655.

    Protein family/group databases

    CAZyiGH68. Glycoside Hydrolase Family 68.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Levansucrase (EC:2.4.1.10)
    Alternative name(s):
    Beta-D-fructofuranosyl transferase
    Sucrose 6-fructosyl transferase
    Gene namesi
    Name:sacB
    Ordered Locus Names:BSU34450
    OrganismiBacillus subtilis (strain 168)
    Taxonomic identifieri224308 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    ProteomesiUP000001570: Chromosome

    Organism-specific databases

    GenoListiBSU34450. [Micado]

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2929Add
    BLAST
    Chaini30 – 473444LevansucrasePRO_0000012249Add
    BLAST

    Proteomic databases

    PaxDbiP05655.

    Expressioni

    Inductioni

    Induced by sucrose.1 Publication

    Interactioni

    Protein-protein interaction databases

    STRINGi224308.BSU34450.

    Structurei

    Secondary structure

    1
    473
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi43 – 453
    Helixi48 – 525
    Helixi54 – 574
    Helixi61 – 633
    Helixi70 – 723
    Helixi77 – 793
    Beta strandi83 – 919
    Beta strandi95 – 973
    Beta strandi103 – 1119
    Beta strandi120 – 1278
    Helixi133 – 1353
    Beta strandi137 – 1426
    Helixi147 – 1504
    Helixi156 – 1583
    Beta strandi161 – 1699
    Beta strandi175 – 1839
    Turni184 – 1885
    Beta strandi189 – 20113
    Beta strandi206 – 21813
    Beta strandi222 – 2254
    Helixi228 – 2347
    Helixi236 – 2394
    Beta strandi246 – 2538
    Beta strandi256 – 26510
    Beta strandi267 – 2693
    Helixi274 – 2785
    Helixi280 – 2823
    Helixi287 – 29913
    Helixi303 – 3086
    Beta strandi311 – 3188
    Beta strandi322 – 33312
    Turni335 – 3373
    Beta strandi342 – 3498
    Beta strandi352 – 3609
    Helixi361 – 3633
    Beta strandi374 – 38310
    Helixi391 – 3933
    Beta strandi395 – 4006
    Beta strandi404 – 4063
    Beta strandi410 – 4167
    Beta strandi419 – 43113
    Beta strandi435 – 4384
    Beta strandi441 – 4433
    Beta strandi447 – 4526
    Beta strandi455 – 4584

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1OYGX-ray1.50A30-473[»]
    1PT2X-ray2.10A30-473[»]
    2VDTX-ray3.20A34-472[»]
    3BYJX-ray2.10A1-473[»]
    3BYKX-ray2.10A1-473[»]
    3BYLX-ray2.10A1-473[»]
    3BYNX-ray2.10A1-473[»]
    ProteinModelPortaliP05655.
    SMRiP05655. Positions 34-473.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP05655.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 68 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG70950.
    HOGENOMiHOG000041220.
    KOiK00692.
    OMAiDIYMLGY.
    OrthoDBiEOG6038XR.
    PhylomeDBiP05655.

    Family and domain databases

    Gene3Di2.115.10.20. 1 hit.
    InterProiIPR003469. Glyco_hydro_68.
    IPR023296. Glyco_hydro_beta-prop.
    [Graphical view]
    PfamiPF02435. Glyco_hydro_68. 1 hit.
    [Graphical view]
    SUPFAMiSSF75005. SSF75005. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P05655-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNIKKFAKQA TVLTFTTALL AGGATQAFAK ETNQKPYKET YGISHITRHD    50
    MLQIPEQQKN EKYQVPEFDS STIKNISSAK GLDVWDSWPL QNADGTVANY 100
    HGYHIVFALA GDPKNADDTS IYMFYQKVGE TSIDSWKNAG RVFKDSDKFD 150
    ANDSILKDQT QEWSGSATFT SDGKIRLFYT DFSGKHYGKQ TLTTAQVNVS 200
    ASDSSLNING VEDYKSIFDG DGKTYQNVQQ FIDEGNYSSG DNHTLRDPHY 250
    VEDKGHKYLV FEANTGTEDG YQGEESLFNK AYYGKSTSFF RQESQKLLQS 300
    DKKRTAELAN GALGMIELND DYTLKKVMKP LIASNTVTDE IERANVFKMN 350
    GKWYLFTDSR GSKMTIDGIT SNDIYMLGYV SNSLTGPYKP LNKTGLVLKM 400
    DLDPNDVTFT YSHFAVPQAK GNNVVITSYM TNRGFYADKQ STFAPSFLLN 450
    IKGKKTSVVK DSILEQGQLT VNK 473
    Length:473
    Mass (Da):52,971
    Last modified:November 1, 1988 - v1
    Checksum:i3FBF2F571B41D5B0
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti12 – 121V → I in AAA22724. (PubMed:6424671)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M14202 Genomic DNA. Translation: AAA22725.1.
    Z94043 Genomic DNA. Translation: CAB08015.1.
    AL009126 Genomic DNA. Translation: CAB15450.1.
    K01987 Genomic DNA. Translation: AAA22724.1.
    X02730 Genomic DNA. Translation: CAA26513.1.
    PIRiS07309. A25040.
    RefSeqiNP_391325.1. NC_000964.3.

    Genome annotation databases

    EnsemblBacteriaiCAB15450; CAB15450; BSU34450.
    GeneIDi936413.
    KEGGibsu:BSU34450.
    PATRICi18978892. VBIBacSub10457_3609.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M14202 Genomic DNA. Translation: AAA22725.1 .
    Z94043 Genomic DNA. Translation: CAB08015.1 .
    AL009126 Genomic DNA. Translation: CAB15450.1 .
    K01987 Genomic DNA. Translation: AAA22724.1 .
    X02730 Genomic DNA. Translation: CAA26513.1 .
    PIRi S07309. A25040.
    RefSeqi NP_391325.1. NC_000964.3.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1OYG X-ray 1.50 A 30-473 [» ]
    1PT2 X-ray 2.10 A 30-473 [» ]
    2VDT X-ray 3.20 A 34-472 [» ]
    3BYJ X-ray 2.10 A 1-473 [» ]
    3BYK X-ray 2.10 A 1-473 [» ]
    3BYL X-ray 2.10 A 1-473 [» ]
    3BYN X-ray 2.10 A 1-473 [» ]
    ProteinModelPortali P05655.
    SMRi P05655. Positions 34-473.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224308.BSU34450.

    Protein family/group databases

    CAZyi GH68. Glycoside Hydrolase Family 68.

    Proteomic databases

    PaxDbi P05655.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAB15450 ; CAB15450 ; BSU34450 .
    GeneIDi 936413.
    KEGGi bsu:BSU34450.
    PATRICi 18978892. VBIBacSub10457_3609.

    Organism-specific databases

    GenoListi BSU34450. [Micado ]

    Phylogenomic databases

    eggNOGi NOG70950.
    HOGENOMi HOG000041220.
    KOi K00692.
    OMAi DIYMLGY.
    OrthoDBi EOG6038XR.
    PhylomeDBi P05655.

    Enzyme and pathway databases

    BioCyci BSUB:BSU34450-MONOMER.
    MetaCyc:BSU34450-MONOMER.
    SABIO-RK P05655.

    Miscellaneous databases

    EvolutionaryTracei P05655.

    Family and domain databases

    Gene3Di 2.115.10.20. 1 hit.
    InterProi IPR003469. Glyco_hydro_68.
    IPR023296. Glyco_hydro_beta-prop.
    [Graphical view ]
    Pfami PF02435. Glyco_hydro_68. 1 hit.
    [Graphical view ]
    SUPFAMi SSF75005. SSF75005. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The DNA sequence of the gene for the secreted Bacillus subtilis enzyme levansucrase and its genetic control sites."
      Steinmetz M., Le Coq D., Aymerich S., Gonzy-Treboul G., Gay P.
      Mol. Gen. Genet. 200:220-228(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 168.
    2. Denizot F.
      Submitted (APR-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 168.
    3. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
      Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
      , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
      Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 168.
    4. "Characterization of the precursor form of the exocellular levansucrase from Bacillus subtilis."
      Fouet A., Arnaud M., Klier A., Rapoport G.
      Biochem. Biophys. Res. Commun. 119:795-800(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-62.
      Strain: 168 / PY79.
    5. "Modulation of Bacillus subtilis levansucrase gene expression by sucrose and regulation of the steady-state mRNA level by sacU and sacQ genes."
      Shimotsu H., Henner D.J.
      J. Bacteriol. 168:380-388(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-68.
    6. "yveB, encoding endolevanase LevB, is part of the sacB-yveB-yveA levansucrase tricistronic operon in Bacillus subtilis."
      Pereira Y., Petit-Glatron M.-F., Chambert R.
      Microbiology 147:3413-3419(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.

    Entry informationi

    Entry nameiSACB_BACSU
    AccessioniPrimary (citable) accession number: P05655
    Secondary accession number(s): P70984
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1988
    Last sequence update: November 1, 1988
    Last modified: October 1, 2014
    This is version 112 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Bacillus subtilis
      Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3