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P05655 (SACB_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Levansucrase
EC=2.4.1.10
Alternative name(s):
Beta-D-fructofuranosyl transferase
Sucrose 6-fructosyl transferase
Gene names
Name:sacB
Ordered Locus Names:BSU34450
OrganismBacillus subtilis (strain 168) [Reference proteome] [HAMAP]
Taxonomic identifier224308 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus ›

Protein attributes

Sequence length473 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Sucrose + (6)-beta-D-fructofuranosyl-(2->)(n) alpha-D-glucopyranoside = glucose + (6)-beta-D-fructofuranosyl-(2->)(n+1) alpha-D-glucopyranoside.

Subcellular location

Secreted.

Induction

Induced by sucrose. Ref.6

Sequence similarities

Belongs to the glycosyl hydrolase 68 family.

Ontologies

Keywords
   Cellular componentSecreted
   DomainSignal
   Molecular functionGlycosyltransferase
Transferase
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcarbohydrate utilization

Inferred from electronic annotation. Source: InterPro

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionlevansucrase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2929
Chain30 – 473444Levansucrase
PRO_0000012249

Experimental info

Sequence conflict121V → I in AAA22724. Ref.4

Secondary structure

1 ........................................................................................ 473
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P05655 [UniParc].

Last modified November 1, 1988. Version 1.
Checksum: 3FBF2F571B41D5B0

FASTA47352,971
        10         20         30         40         50         60 
MNIKKFAKQA TVLTFTTALL AGGATQAFAK ETNQKPYKET YGISHITRHD MLQIPEQQKN 

        70         80         90        100        110        120 
EKYQVPEFDS STIKNISSAK GLDVWDSWPL QNADGTVANY HGYHIVFALA GDPKNADDTS 

       130        140        150        160        170        180 
IYMFYQKVGE TSIDSWKNAG RVFKDSDKFD ANDSILKDQT QEWSGSATFT SDGKIRLFYT 

       190        200        210        220        230        240 
DFSGKHYGKQ TLTTAQVNVS ASDSSLNING VEDYKSIFDG DGKTYQNVQQ FIDEGNYSSG 

       250        260        270        280        290        300 
DNHTLRDPHY VEDKGHKYLV FEANTGTEDG YQGEESLFNK AYYGKSTSFF RQESQKLLQS 

       310        320        330        340        350        360 
DKKRTAELAN GALGMIELND DYTLKKVMKP LIASNTVTDE IERANVFKMN GKWYLFTDSR 

       370        380        390        400        410        420 
GSKMTIDGIT SNDIYMLGYV SNSLTGPYKP LNKTGLVLKM DLDPNDVTFT YSHFAVPQAK 

       430        440        450        460        470 
GNNVVITSYM TNRGFYADKQ STFAPSFLLN IKGKKTSVVK DSILEQGQLT VNK

« Hide

References

« Hide 'large scale' references
[1]"The DNA sequence of the gene for the secreted Bacillus subtilis enzyme levansucrase and its genetic control sites."
Steinmetz M., Le Coq D., Aymerich S., Gonzy-Treboul G., Gay P.
Mol. Gen. Genet. 200:220-228(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168.
[2]Denizot F.
Submitted (APR-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168.
[3]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[4]"Characterization of the precursor form of the exocellular levansucrase from Bacillus subtilis."
Fouet A., Arnaud M., Klier A., Rapoport G.
Biochem. Biophys. Res. Commun. 119:795-800(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-62.
Strain: 168 / PY79.
[5]"Modulation of Bacillus subtilis levansucrase gene expression by sucrose and regulation of the steady-state mRNA level by sacU and sacQ genes."
Shimotsu H., Henner D.J.
J. Bacteriol. 168:380-388(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-68.
[6]"yveB, encoding endolevanase LevB, is part of the sacB-yveB-yveA levansucrase tricistronic operon in Bacillus subtilis."
Pereira Y., Petit-Glatron M.-F., Chambert R.
Microbiology 147:3413-3419(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M14202 Genomic DNA. Translation: AAA22725.1.
Z94043 Genomic DNA. Translation: CAB08015.1.
AL009126 Genomic DNA. Translation: CAB15450.1.
K01987 Genomic DNA. Translation: AAA22724.1.
X02730 Genomic DNA. Translation: CAA26513.1.
PIRA25040. S07309.
RefSeqNP_391325.1. NC_000964.3.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1OYGX-ray1.50A30-473[»]
1PT2X-ray2.10A30-473[»]
2VDTX-ray3.20A34-472[»]
3BYJX-ray2.10A1-473[»]
3BYKX-ray2.10A1-473[»]
3BYLX-ray2.10A1-473[»]
3BYNX-ray2.10A1-473[»]
ProteinModelPortalP05655.
SMRP05655. Positions 34-473.
ModBaseSearch...

Protein-protein interaction databases

STRING224308.BSU34450.

Protein family/group databases

CAZyGH68. Glycoside Hydrolase Family 68.

Proteomic databases

PaxDbP05655.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB15450; CAB15450; BSU34450.
GeneID936413.
KEGGbsu:BSU34450.
PATRIC18978892. VBIBacSub10457_3609.

Organism-specific databases

GenoListBSU34450. [Micado]

Phylogenomic databases

eggNOGNOG70950.
HOGENOMHOG000041220.
KOK00692.
OMAWKNAGRV.
ProtClustDBCLSK882932.

Enzyme and pathway databases

BioCycBSUB:BSU34450-MONOMER.
MetaCyc:BSU34450-MONOMER.

Family and domain databases

Gene3D2.115.10.20. 1 hit.
InterProIPR003469. Glyco_hydro_68.
IPR023296. Glyco_hydro_beta-prop.
[Graphical view]
PfamPF02435. Glyco_hydro_68. 1 hit.
[Graphical view]
SUPFAMSSF75005. Glyco_hydro_43_beta-prop. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP05655.

Entry information

Entry nameSACB_BACSU
AccessionPrimary (citable) accession number: P05655
Secondary accession number(s): P70984
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: November 1, 1988
Last modified: May 1, 2013
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents