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Protein

Aspartate carbamoyltransferase

Gene

pyrB

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate.

Pathwayi: UMP biosynthesis via de novo pathway

This protein is involved in step 2 of the subpathway that synthesizes (S)-dihydroorotate from bicarbonate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Carbamoyl-phosphate synthase pyrimidine-specific large chain (pyrAB), Carbamoyl-phosphate synthase pyrimidine-specific small chain (pyrAA)
  2. Aspartate carbamoyltransferase (pyrB)
  3. Dihydroorotase (pyrC)
This subpathway is part of the pathway UMP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-dihydroorotate from bicarbonate, the pathway UMP biosynthesis via de novo pathway and in Pyrimidine metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Pyrimidine biosynthesis

Enzyme and pathway databases

BioCyciBSUB:BSU15490-MONOMER.
BRENDAi2.1.3.2. 658.
SABIO-RKP05654.
UniPathwayiUPA00070; UER00116.

Names & Taxonomyi

Protein namesi
Recommended name:
Aspartate carbamoyltransferase (EC:2.1.3.2)
Alternative name(s):
Aspartate transcarbamylase
Short name:
ATCase
Gene namesi
Name:pyrB
Ordered Locus Names:BSU15490
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001130991 – 304Aspartate carbamoyltransferaseAdd BLAST304

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei303Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP05654.
PRIDEiP05654.

PTM databases

iPTMnetiP05654.

Interactioni

Subunit structurei

Homotrimer.

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100008561.

Structurei

Secondary structure

1304
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi7 – 9Combined sources3
Helixi12 – 26Combined sources15
Turni33 – 36Combined sources4
Beta strandi38 – 45Combined sources8
Helixi49 – 60Combined sources12
Beta strandi64 – 69Combined sources6
Helixi73 – 76Combined sources4
Beta strandi77 – 79Combined sources3
Helixi81 – 91Combined sources11
Beta strandi95 – 99Combined sources5
Turni103 – 105Combined sources3
Helixi106 – 112Combined sources7
Beta strandi117 – 120Combined sources4
Helixi128 – 142Combined sources15
Beta strandi149 – 154Combined sources6
Helixi156 – 158Combined sources3
Helixi160 – 171Combined sources12
Beta strandi175 – 180Combined sources6
Helixi182 – 184Combined sources3
Beta strandi192 – 194Combined sources3
Helixi197 – 203Combined sources7
Beta strandi205 – 209Combined sources5
Turni214 – 216Combined sources3
Helixi220 – 224Combined sources5
Helixi226 – 230Combined sources5
Helixi234 – 237Combined sources4
Beta strandi245 – 247Combined sources3
Turni254 – 256Combined sources3
Helixi260 – 262Combined sources3
Helixi270 – 289Combined sources20

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2AT2X-ray3.00A/B/C1-300[»]
3R7DX-ray2.20A/B/C1-304[»]
3R7FX-ray2.10A/B/C1-304[»]
3R7LX-ray2.58A/B/C/D/E/F1-304[»]
ProteinModelPortaliP05654.
SMRiP05654.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP05654.

Family & Domainsi

Sequence similaritiesi

Belongs to the ATCase/OTCase family.Curated

Phylogenomic databases

eggNOGiENOG4105CXT. Bacteria.
COG0540. LUCA.
HOGENOMiHOG000022685.
InParanoidiP05654.
KOiK00609.
OMAiGPINREI.
PhylomeDBiP05654.

Family and domain databases

Gene3Di3.40.50.1370. 2 hits.
HAMAPiMF_00001. Asp_carb_tr. 1 hit.
InterProiIPR006132. Asp/Orn_carbamoyltranf_P-bd.
IPR006130. Asp/Orn_carbamoylTrfase.
IPR002082. Asp_carbamoyltransf.
IPR006131. Asp_carbamoyltransf_Asp/Orn-bd.
[Graphical view]
PfamiPF00185. OTCace. 1 hit.
PF02729. OTCace_N. 1 hit.
[Graphical view]
PRINTSiPR00100. AOTCASE.
PR00101. ATCASE.
SUPFAMiSSF53671. SSF53671. 1 hit.
TIGRFAMsiTIGR00670. asp_carb_tr. 1 hit.
PROSITEiPS00097. CARBAMOYLTRANSFERASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P05654-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKHLTTMSEL STEEIKDLLQ TAQELKSGKT DNQLTGKFAA NLFFEPSTRT
60 70 80 90 100
RFSFEVAEKK LGMNVLNLDG TSTSVQKGET LYDTIRTLES IGVDVCVIRH
110 120 130 140 150
SEDEYYEELV SQVNIPILNA GDGCGQHPTQ SLLDLMTIYE EFNTFKGLTV
160 170 180 190 200
SIHGDIKHSR VARSNAEVLT RLGARVLFSG PSEWQDEENT FGTYVSMDEA
210 220 230 240 250
VESSDVVMLL RIQNERHQSA VSQEGYLNKY GLTVERAERM KRHAIIMHPA
260 270 280 290 300
PVNRGVEIDD SLVESEKSRI FKQMKNGVFI RMAVIQRALQ TNVKRGEAAY

VISH
Length:304
Mass (Da):34,224
Last modified:June 16, 2009 - v2
Checksum:i3742CA07346E15FE
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti287R → C in AAA22685 (PubMed:3015959).Curated1
Sequence conflicti287R → C in AAA21267 (PubMed:1709162).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M13128 Genomic DNA. Translation: AAA22685.1.
M59757 Genomic DNA. Translation: AAA21267.1.
AL009126 Genomic DNA. Translation: CAB13423.2.
PIRiA25015. OWBSAC.
RefSeqiNP_389432.2. NC_000964.3.
WP_003245123.1. NZ_JNCM01000035.1.

Genome annotation databases

EnsemblBacteriaiCAB13423; CAB13423; BSU15490.
GeneIDi937734.
KEGGibsu:BSU15490.
PATRICi18974905. VBIBacSub10457_1644.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M13128 Genomic DNA. Translation: AAA22685.1.
M59757 Genomic DNA. Translation: AAA21267.1.
AL009126 Genomic DNA. Translation: CAB13423.2.
PIRiA25015. OWBSAC.
RefSeqiNP_389432.2. NC_000964.3.
WP_003245123.1. NZ_JNCM01000035.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2AT2X-ray3.00A/B/C1-300[»]
3R7DX-ray2.20A/B/C1-304[»]
3R7FX-ray2.10A/B/C1-304[»]
3R7LX-ray2.58A/B/C/D/E/F1-304[»]
ProteinModelPortaliP05654.
SMRiP05654.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100008561.

PTM databases

iPTMnetiP05654.

Proteomic databases

PaxDbiP05654.
PRIDEiP05654.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB13423; CAB13423; BSU15490.
GeneIDi937734.
KEGGibsu:BSU15490.
PATRICi18974905. VBIBacSub10457_1644.

Phylogenomic databases

eggNOGiENOG4105CXT. Bacteria.
COG0540. LUCA.
HOGENOMiHOG000022685.
InParanoidiP05654.
KOiK00609.
OMAiGPINREI.
PhylomeDBiP05654.

Enzyme and pathway databases

UniPathwayiUPA00070; UER00116.
BioCyciBSUB:BSU15490-MONOMER.
BRENDAi2.1.3.2. 658.
SABIO-RKP05654.

Miscellaneous databases

EvolutionaryTraceiP05654.

Family and domain databases

Gene3Di3.40.50.1370. 2 hits.
HAMAPiMF_00001. Asp_carb_tr. 1 hit.
InterProiIPR006132. Asp/Orn_carbamoyltranf_P-bd.
IPR006130. Asp/Orn_carbamoylTrfase.
IPR002082. Asp_carbamoyltransf.
IPR006131. Asp_carbamoyltransf_Asp/Orn-bd.
[Graphical view]
PfamiPF00185. OTCace. 1 hit.
PF02729. OTCace_N. 1 hit.
[Graphical view]
PRINTSiPR00100. AOTCASE.
PR00101. ATCASE.
SUPFAMiSSF53671. SSF53671. 1 hit.
TIGRFAMsiTIGR00670. asp_carb_tr. 1 hit.
PROSITEiPS00097. CARBAMOYLTRANSFERASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPYRB_BACSU
AccessioniPrimary (citable) accession number: P05654
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: June 16, 2009
Last modified: November 2, 2016
This is version 144 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.