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P05653

- GYRA_BACSU

UniProt

P05653 - GYRA_BACSU

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Protein
DNA gyrase subunit A
Gene
gyrA, cafB, nalA, BSU00070
Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings By similarity.UniRule annotation

Catalytic activityi

ATP-dependent breakage, passage and rejoining of double-stranded DNA.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei123 – 1231O-(5'-phospho-DNA)-tyrosine intermediate By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. DNA binding Source: UniProtKB-HAMAP
  3. DNA topoisomerase type II (ATP-hydrolyzing) activity Source: RefGenome

GO - Biological processi

  1. ATP catabolic process Source: GOC
  2. DNA topological change Source: RefGenome
  3. DNA unwinding involved in DNA replication Source: RefGenome
  4. chromosome segregation Source: RefGenome
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBSUB:BSU00070-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA gyrase subunit A (EC:5.99.1.3)
Gene namesi
Name:gyrA
Synonyms:cafB, nalA
Ordered Locus Names:BSU00070
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001570: Chromosome

Organism-specific databases

GenoListiBSU00070. [Micado]

Subcellular locationi

Cytoplasm Reviewed prediction UniRule annotation

GO - Cellular componenti

  1. DNA topoisomerase complex (ATP-hydrolyzing) Source: RefGenome
  2. chromosome Source: InterPro
  3. cytoplasm Source: UniProtKB-SubCell
  4. nucleoid Source: RefGenome
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 821821DNA gyrase subunit AUniRule annotation
PRO_0000145221Add
BLAST

Proteomic databases

PaxDbiP05653.

Interactioni

Subunit structurei

Heterotetramer, composed of two GyrA and two GyrB chains. Within the heterotetramer, GyrA contains the active site tyrosine that forms a covalent intermediate with the DNA, while GyrB contributes the cofactor binding sites and catalyzes ATP hydrolysis By similarity.

Protein-protein interaction databases

IntActiP05653. 5 interactions.
MINTiMINT-8366974.
STRINGi224308.BSU00070.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni38 – 403
Helixi44 – 5512
Beta strandi60 – 623
Beta strandi64 – 663
Helixi67 – 7711
Helixi82 – 9211
Turni96 – 983
Beta strandi103 – 1086
Beta strandi125 – 1284
Helixi132 – 1365
Beta strandi146 – 1483
Beta strandi152 – 1598
Helixi166 – 1705
Beta strandi172 – 1765
Beta strandi179 – 1835
Helixi188 – 20013
Helixi206 – 2105
Beta strandi222 – 2243
Helixi227 – 23610
Beta strandi237 – 2448
Beta strandi246 – 2516
Beta strandi257 – 2637
Helixi270 – 28213
Beta strandi289 – 2946
Beta strandi303 – 3075
Helixi313 – 32311
Beta strandi324 – 33310
Beta strandi335 – 34410
Helixi347 – 38842
Helixi390 – 3989
Helixi403 – 41412
Helixi418 – 4258
Helixi429 – 4324
Helixi434 – 45926
Helixi461 – 47919
Beta strandi485 – 4873

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4DDQX-ray3.30A/B/C/D/E/F1-502[»]
ProteinModelPortaliP05653.
SMRiP05653. Positions 11-809.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0188.
HOGENOMiHOG000076278.
KOiK02469.
OMAiQTVFGIN.
OrthoDBiEOG661H5V.
PhylomeDBiP05653.

Family and domain databases

Gene3Di3.30.1360.40. 1 hit.
3.90.199.10. 2 hits.
HAMAPiMF_01897. GyrA.
InterProiIPR024946. Arg_repress_C-like.
IPR005743. GyrA.
IPR006691. GyrA/parC_pinwhl.
IPR002205. Topo_IIA_A/C.
IPR013758. Topo_IIA_A/C_ab.
IPR013760. Topo_IIA_like_dom.
[Graphical view]
PfamiPF03989. DNA_gyraseA_C. 6 hits.
PF00521. DNA_topoisoIV. 1 hit.
[Graphical view]
SMARTiSM00434. TOP4c. 1 hit.
[Graphical view]
SUPFAMiSSF56719. SSF56719. 1 hit.
TIGRFAMsiTIGR01063. gyrA. 1 hit.

Sequencei

Sequence statusi: Complete.

P05653-1 [UniParc]FASTAAdd to Basket

« Hide

MSEQNTPQVR EINISQEMRT SFLDYAMSVI VSRALPDVRD GLKPVHRRIL    50
YAMNDLGMTS DKPYKKSARI VGEVIGKYHP HGDSAVYESM VRMAQDFNYR 100
YMLVDGHGNF GSVDGDSAAA MRYTEARMSK ISMEILRDIT KDTIDYQDNY 150
DGSEREPVVM PSRFPNLLVN GAAGIAVGMA TNIPPHQLGE IIDGVLAVSE 200
NPDITIPELM EVIPGPDFPT AGQILGRSGI RKAYESGRGS ITIRAKAEIE 250
QTSSGKERII VTELPYQVNK AKLIEKIADL VRDKKIEGIT DLRDESDRTG 300
MRIVIEIRRD ANANVILNNL YKQTALQTSF GINLLALVDG QPKVLTLKQC 350
LEHYLDHQKV VIRRRTAYEL RKAEARAHIL EGLRVALDHL DAVISLIRNS 400
QTAEIARTGL IEQFSLTEKQ AQAILDMRLQ RLTGLEREKI EEEYQSLVKL 450
IAELKDILAN EYKVLEIIRE ELTEIKERFN DERRTEIVTS GLETIEDEDL 500
IERENIVVTL THNGYVKRLP ASTYRSQKRG GKGVQGMGTN EDDFVEHLIS 550
TSTHDTILFF SNKGKVYRAK GYEIPEYGRT AKGIPIINLL EVEKGEWINA 600
IIPVTEFNAE LYLFFTTKHG VSKRTSLSQF ANIRNNGLIA LSLREDDELM 650
GVRLTDGTKQ IIIGTKNGLL IRFPETDVRE MGRTAAGVKG ITLTDDDVVV 700
GMEILEEESH VLIVTEKGYG KRTPAEEYRT QSRGGKGLKT AKITENNGQL 750
VAVKATKGEE DLMIITASGV LIRMDINDIS ITGRVTQGVR LIRMAEEEHV 800
ATVALVEKNE EDENEEEQEE V 821
Length:821
Mass (Da):92,099
Last modified:November 1, 1988 - v1
Checksum:i717847A5F35FB857
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X02369 Genomic DNA. Translation: CAA26222.1.
D26185 Genomic DNA. Translation: BAA05243.1.
AL009126 Genomic DNA. Translation: CAB11783.1.
PIRiF22930.
RefSeqiNP_387888.1. NC_000964.3.

Genome annotation databases

EnsemblBacteriaiCAB11783; CAB11783; BSU00070.
GeneIDi940002.
KEGGibsu:BSU00070.
PATRICi18971463. VBIBacSub10457_0008.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X02369 Genomic DNA. Translation: CAA26222.1 .
D26185 Genomic DNA. Translation: BAA05243.1 .
AL009126 Genomic DNA. Translation: CAB11783.1 .
PIRi F22930.
RefSeqi NP_387888.1. NC_000964.3.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4DDQ X-ray 3.30 A/B/C/D/E/F 1-502 [» ]
ProteinModelPortali P05653.
SMRi P05653. Positions 11-809.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P05653. 5 interactions.
MINTi MINT-8366974.
STRINGi 224308.BSU00070.

Chemistry

BindingDBi P05653.

Proteomic databases

PaxDbi P05653.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAB11783 ; CAB11783 ; BSU00070 .
GeneIDi 940002.
KEGGi bsu:BSU00070.
PATRICi 18971463. VBIBacSub10457_0008.

Organism-specific databases

GenoListi BSU00070. [Micado ]

Phylogenomic databases

eggNOGi COG0188.
HOGENOMi HOG000076278.
KOi K02469.
OMAi QTVFGIN.
OrthoDBi EOG661H5V.
PhylomeDBi P05653.

Enzyme and pathway databases

BioCyci BSUB:BSU00070-MONOMER.

Miscellaneous databases

PROi P05653.

Family and domain databases

Gene3Di 3.30.1360.40. 1 hit.
3.90.199.10. 2 hits.
HAMAPi MF_01897. GyrA.
InterProi IPR024946. Arg_repress_C-like.
IPR005743. GyrA.
IPR006691. GyrA/parC_pinwhl.
IPR002205. Topo_IIA_A/C.
IPR013758. Topo_IIA_A/C_ab.
IPR013760. Topo_IIA_like_dom.
[Graphical view ]
Pfami PF03989. DNA_gyraseA_C. 6 hits.
PF00521. DNA_topoisoIV. 1 hit.
[Graphical view ]
SMARTi SM00434. TOP4c. 1 hit.
[Graphical view ]
SUPFAMi SSF56719. SSF56719. 1 hit.
TIGRFAMsi TIGR01063. gyrA. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Structure and function of the region of the replication origin of the Bacillus subtilis chromosome. III. Nucleotide sequence of some 10,000 base pairs in the origin region."
    Moriya S., Ogasawara N., Yoshikawa H.
    Nucleic Acids Res. 13:2251-2265(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Systematic sequencing of the 180 kilobase region of the Bacillus subtilis chromosome containing the replication origin."
    Ogasawara N., Nakai S., Yoshikawa H.
    DNA Res. 1:1-14(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168.
  3. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.

Entry informationi

Entry nameiGYRA_BACSU
AccessioniPrimary (citable) accession number: P05653
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: November 1, 1988
Last modified: September 3, 2014
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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