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P05653 (GYRA_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA gyrase subunit A

EC=5.99.1.3
Gene names
Name:gyrA
Synonyms:cafB, nalA
Ordered Locus Names:BSU00070
OrganismBacillus subtilis (strain 168) [Reference proteome] [HAMAP]
Taxonomic identifier224308 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length821 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings By similarity. HAMAP-Rule MF_01897

Catalytic activity

ATP-dependent breakage, passage and rejoining of double-stranded DNA. HAMAP-Rule MF_01897

Subunit structure

Heterotetramer, composed of two GyrA and two GyrB chains. Within the heterotetramer, GyrA contains the active site tyrosine that forms a covalent intermediate with the DNA, while GyrB contributes the cofactor binding sites and catalyzes ATP hydrolysis By similarity.

Subcellular location

Cytoplasm Potential HAMAP-Rule MF_01897.

Sequence similarities

Belongs to the topoisomerase GyrA/ParC subunit family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 821821DNA gyrase subunit A HAMAP-Rule MF_01897
PRO_0000145221

Sites

Active site1231O-(5'-phospho-DNA)-tyrosine intermediate By similarity

Secondary structure

...................................................................... 821
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P05653 [UniParc].

Last modified November 1, 1988. Version 1.
Checksum: 717847A5F35FB857

FASTA82192,099
        10         20         30         40         50         60 
MSEQNTPQVR EINISQEMRT SFLDYAMSVI VSRALPDVRD GLKPVHRRIL YAMNDLGMTS 

        70         80         90        100        110        120 
DKPYKKSARI VGEVIGKYHP HGDSAVYESM VRMAQDFNYR YMLVDGHGNF GSVDGDSAAA 

       130        140        150        160        170        180 
MRYTEARMSK ISMEILRDIT KDTIDYQDNY DGSEREPVVM PSRFPNLLVN GAAGIAVGMA 

       190        200        210        220        230        240 
TNIPPHQLGE IIDGVLAVSE NPDITIPELM EVIPGPDFPT AGQILGRSGI RKAYESGRGS 

       250        260        270        280        290        300 
ITIRAKAEIE QTSSGKERII VTELPYQVNK AKLIEKIADL VRDKKIEGIT DLRDESDRTG 

       310        320        330        340        350        360 
MRIVIEIRRD ANANVILNNL YKQTALQTSF GINLLALVDG QPKVLTLKQC LEHYLDHQKV 

       370        380        390        400        410        420 
VIRRRTAYEL RKAEARAHIL EGLRVALDHL DAVISLIRNS QTAEIARTGL IEQFSLTEKQ 

       430        440        450        460        470        480 
AQAILDMRLQ RLTGLEREKI EEEYQSLVKL IAELKDILAN EYKVLEIIRE ELTEIKERFN 

       490        500        510        520        530        540 
DERRTEIVTS GLETIEDEDL IERENIVVTL THNGYVKRLP ASTYRSQKRG GKGVQGMGTN 

       550        560        570        580        590        600 
EDDFVEHLIS TSTHDTILFF SNKGKVYRAK GYEIPEYGRT AKGIPIINLL EVEKGEWINA 

       610        620        630        640        650        660 
IIPVTEFNAE LYLFFTTKHG VSKRTSLSQF ANIRNNGLIA LSLREDDELM GVRLTDGTKQ 

       670        680        690        700        710        720 
IIIGTKNGLL IRFPETDVRE MGRTAAGVKG ITLTDDDVVV GMEILEEESH VLIVTEKGYG 

       730        740        750        760        770        780 
KRTPAEEYRT QSRGGKGLKT AKITENNGQL VAVKATKGEE DLMIITASGV LIRMDINDIS 

       790        800        810        820 
ITGRVTQGVR LIRMAEEEHV ATVALVEKNE EDENEEEQEE V 

« Hide

References

« Hide 'large scale' references
[1]"Structure and function of the region of the replication origin of the Bacillus subtilis chromosome. III. Nucleotide sequence of some 10,000 base pairs in the origin region."
Moriya S., Ogasawara N., Yoshikawa H.
Nucleic Acids Res. 13:2251-2265(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Systematic sequencing of the 180 kilobase region of the Bacillus subtilis chromosome containing the replication origin."
Ogasawara N., Nakai S., Yoshikawa H.
DNA Res. 1:1-14(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168.
[3]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X02369 Genomic DNA. Translation: CAA26222.1.
D26185 Genomic DNA. Translation: BAA05243.1.
AL009126 Genomic DNA. Translation: CAB11783.1.
PIRF22930.
RefSeqNP_387888.1. NC_000964.3.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4DDQX-ray3.30A/B/C/D/E/F1-502[»]
ProteinModelPortalP05653.
SMRP05653. Positions 11-809.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP05653. 5 interactions.
MINTMINT-8366974.
STRING224308.BSU00070.

Chemistry

BindingDBP05653.

Proteomic databases

PaxDbP05653.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB11783; CAB11783; BSU00070.
GeneID940002.
KEGGbsu:BSU00070.
PATRIC18971463. VBIBacSub10457_0008.

Organism-specific databases

GenoListBSU00070. [Micado]

Phylogenomic databases

eggNOGCOG0188.
HOGENOMHOG000076278.
KOK02469.
OMAETVDWVP.
OrthoDBEOG661H5V.
ProtClustDBPRK05560.

Enzyme and pathway databases

BioCycBSUB:BSU00070-MONOMER.

Family and domain databases

Gene3D3.30.1360.40. 1 hit.
3.90.199.10. 2 hits.
HAMAPMF_01897. GyrA.
InterProIPR024946. Arg_repress_C-like.
IPR005743. GyrA.
IPR006691. GyrA/parC_pinwhl.
IPR002205. Topo_IIA_A/C.
IPR013758. Topo_IIA_A/C_ab.
IPR013760. Topo_IIA_like_dom.
[Graphical view]
PfamPF03989. DNA_gyraseA_C. 6 hits.
PF00521. DNA_topoisoIV. 1 hit.
[Graphical view]
SMARTSM00434. TOP4c. 1 hit.
[Graphical view]
SUPFAMSSF56719. SSF56719. 1 hit.
TIGRFAMsTIGR01063. gyrA. 1 hit.
ProtoNetSearch...

Other

PROP05653.

Entry information

Entry nameGYRA_BACSU
AccessionPrimary (citable) accession number: P05653
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: November 1, 1988
Last modified: April 16, 2014
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList