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Protein

DNA replication and repair protein RecF

Gene

recF

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

The RecF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP. Is recruited to repair centers, foci that are the site of double-strand DNA break(s) after RecN and RecO; recruitment may depend on RecO.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi30 – 37ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

  • DNA replication Source: UniProtKB-KW
  • DNA synthesis involved in DNA repair Source: GO_Central
  • double-strand break repair Source: UniProtKB
  • SOS response Source: UniProtKB-KW

Keywordsi

Molecular functionDNA-binding
Biological processDNA damage, DNA repair, DNA replication, SOS response
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBSUB:BSU00040-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
DNA replication and repair protein RecF
Gene namesi
Name:recF
Ordered Locus Names:BSU00040
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

  • nucleoid 1 Publication
  • Note: Cytoplasmically located in untreated cells. Recruited to foci following treatment with DNA damaging agents; these foci are presumably the breaks themselves. They are almost always located within nucleoids.

GO - Cellular componenti

  • bacterial nucleoid Source: UniProtKB
  • cytoplasm Source: UniProtKB-KW

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001963981 – 370DNA replication and repair protein RecFAdd BLAST370

Proteomic databases

PaxDbiP05651
PRIDEiP05651

Interactioni

Subunit structurei

Recruited to foci following DNA damage; probably interacts with RecO.

Protein-protein interaction databases

IntActiP05651, 2 interactors
STRINGi224308.Bsubs1_010100000020

Structurei

3D structure databases

ProteinModelPortaliP05651
SMRiP05651
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RecF family.Curated

Phylogenomic databases

eggNOGiCOG1195 LUCA
HOGENOMiHOG000269559
InParanoidiP05651
KOiK03629
OMAiGQQKSFL
PhylomeDBiP05651

Family and domain databases

HAMAPiMF_00365 RecF, 1 hit
InterProiView protein in InterPro
IPR001238 DNA-binding_RecF
IPR018078 DNA-binding_RecF_CS
IPR027417 P-loop_NTPase
IPR003395 RecF/RecN/SMC_N
PANTHERiPTHR32182:SF0 PTHR32182:SF0, 1 hit
PfamiView protein in Pfam
PF02463 SMC_N, 1 hit
SUPFAMiSSF52540 SSF52540, 2 hits
TIGRFAMsiTIGR00611 recf, 1 hit
PROSITEiView protein in PROSITE
PS00617 RECF_1, 1 hit
PS00618 RECF_2, 1 hit

Sequencei

Sequence statusi: Complete.

P05651-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYIQNLELTS YRNYDHAELQ FENKVNVIIG ENAQGKTNLM EAIYVLSMAK
60 70 80 90 100
SHRTSNDKEL IRWDKDYAKI EGRVMKQNGA IPMQLVISKK GKKGKVNHIE
110 120 130 140 150
QQKLSQYVGA LNTIMFAPED LNLVKGSPQV RRRFLDMEIG QVSPVYLHDL
160 170 180 190 200
SLYQKILSQR NHFLKQLQTR KQTDRTMLDV LTDQLVEVAA KVVVKRLQFT
210 220 230 240 250
AQLEKWAQPI HAGISRGLEE LTLKYHTALD VSDPLDLSKI GDSYQEAFSK
260 270 280 290 300
LREKEIERGV TLSGPHRDDV LFYVNGRDVQ TYGSQGQQRT TALSLKLAEI
310 320 330 340 350
DLIHEEIGEY PILLLDDVLS ELDDYRQSHL LHTIQGRVQT FVTTTSVDGI
360 370
DHETLRQAGM FRVQNGALVK
Length:370
Mass (Da):42,304
Last modified:March 1, 1992 - v2
Checksum:i51ADB3DEA7CDE6E0
GO

Sequence cautioni

The sequence CAA26220 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02369 Genomic DNA Translation: CAA26220.1 Different initiation.
D26185 Genomic DNA Translation: BAA05240.1
AL009126 Genomic DNA Translation: CAB11780.1
PIRiD22930
RefSeqiNP_387885.1, NC_000964.3
WP_003243515.1, NZ_JNCM01000034.1

Genome annotation databases

EnsemblBacteriaiCAB11780; CAB11780; BSU00040
GeneIDi939454
KEGGibsu:BSU00040
PATRICifig|224308.179.peg.4

Similar proteinsi

Entry informationi

Entry nameiRECF_BACSU
AccessioniPrimary (citable) accession number: P05651
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: March 1, 1992
Last modified: May 23, 2018
This is version 130 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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