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Protein

DNA replication and repair protein RecF

Gene

recF

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

The RecF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP. Is recruited to repair centers, foci that are the site of double-strand DNA break(s) after RecN and RecO; recruitment may depend on RecO.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi30 – 378ATPSequence analysis

GO - Molecular functioni

GO - Biological processi

  • DNA replication Source: UniProtKB-HAMAP
  • DNA synthesis involved in DNA repair Source: GO_Central
  • double-strand break repair Source: UniProtKB
  • SOS response Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA repair, DNA replication, SOS response

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBSUB:BSU00040-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA replication and repair protein RecF
Gene namesi
Name:recF
Ordered Locus Names:BSU00040
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

  • Cytoplasmnucleoid 1 Publication

  • Note: Cytoplasmically located in untreated cells. Recruited to foci following treatment with DNA damaging agents; these foci are presumably the breaks themselves. They are almost always located within nucleoids.

GO - Cellular componenti

  • bacterial nucleoid Source: UniProtKB
  • cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 370370DNA replication and repair protein RecFPRO_0000196398Add
BLAST

Proteomic databases

PaxDbiP05651.

Interactioni

Subunit structurei

Recruited to foci following DNA damage; probably interacts with RecO.

Protein-protein interaction databases

IntActiP05651. 2 interactions.
STRINGi224308.Bsubs1_010100000020.

Structurei

3D structure databases

ProteinModelPortaliP05651.
SMRiP05651. Positions 1-368.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RecF family.Curated

Phylogenomic databases

eggNOGiCOG1195. LUCA.
HOGENOMiHOG000269559.
InParanoidiP05651.
KOiK03629.
OMAiKVQTFIT.
PhylomeDBiP05651.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_00365. RecF. 1 hit.
InterProiIPR001238. DNA-binding_RecF.
IPR018078. DNA-binding_RecF_CS.
IPR027417. P-loop_NTPase.
IPR003395. RecF/RecN/SMC_N.
[Graphical view]
PANTHERiPTHR32182:SF0. PTHR32182:SF0. 1 hit.
PfamiPF02463. SMC_N. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
TIGRFAMsiTIGR00611. recf. 1 hit.
PROSITEiPS00617. RECF_1. 1 hit.
PS00618. RECF_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P05651-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYIQNLELTS YRNYDHAELQ FENKVNVIIG ENAQGKTNLM EAIYVLSMAK
60 70 80 90 100
SHRTSNDKEL IRWDKDYAKI EGRVMKQNGA IPMQLVISKK GKKGKVNHIE
110 120 130 140 150
QQKLSQYVGA LNTIMFAPED LNLVKGSPQV RRRFLDMEIG QVSPVYLHDL
160 170 180 190 200
SLYQKILSQR NHFLKQLQTR KQTDRTMLDV LTDQLVEVAA KVVVKRLQFT
210 220 230 240 250
AQLEKWAQPI HAGISRGLEE LTLKYHTALD VSDPLDLSKI GDSYQEAFSK
260 270 280 290 300
LREKEIERGV TLSGPHRDDV LFYVNGRDVQ TYGSQGQQRT TALSLKLAEI
310 320 330 340 350
DLIHEEIGEY PILLLDDVLS ELDDYRQSHL LHTIQGRVQT FVTTTSVDGI
360 370
DHETLRQAGM FRVQNGALVK
Length:370
Mass (Da):42,304
Last modified:March 1, 1992 - v2
Checksum:i51ADB3DEA7CDE6E0
GO

Sequence cautioni

The sequence CAA26220 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02369 Genomic DNA. Translation: CAA26220.1. Different initiation.
D26185 Genomic DNA. Translation: BAA05240.1.
AL009126 Genomic DNA. Translation: CAB11780.1.
PIRiD22930.
RefSeqiNP_387885.1. NC_000964.3.
WP_003243515.1. NZ_JNCM01000034.1.

Genome annotation databases

EnsemblBacteriaiCAB11780; CAB11780; BSU00040.
GeneIDi939454.
KEGGibsu:BSU00040.
PATRICi18971455. VBIBacSub10457_0004.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02369 Genomic DNA. Translation: CAA26220.1. Different initiation.
D26185 Genomic DNA. Translation: BAA05240.1.
AL009126 Genomic DNA. Translation: CAB11780.1.
PIRiD22930.
RefSeqiNP_387885.1. NC_000964.3.
WP_003243515.1. NZ_JNCM01000034.1.

3D structure databases

ProteinModelPortaliP05651.
SMRiP05651. Positions 1-368.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP05651. 2 interactions.
STRINGi224308.Bsubs1_010100000020.

Proteomic databases

PaxDbiP05651.

Protocols and materials databases

DNASUi939454.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB11780; CAB11780; BSU00040.
GeneIDi939454.
KEGGibsu:BSU00040.
PATRICi18971455. VBIBacSub10457_0004.

Phylogenomic databases

eggNOGiCOG1195. LUCA.
HOGENOMiHOG000269559.
InParanoidiP05651.
KOiK03629.
OMAiKVQTFIT.
PhylomeDBiP05651.

Enzyme and pathway databases

BioCyciBSUB:BSU00040-MONOMER.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_00365. RecF. 1 hit.
InterProiIPR001238. DNA-binding_RecF.
IPR018078. DNA-binding_RecF_CS.
IPR027417. P-loop_NTPase.
IPR003395. RecF/RecN/SMC_N.
[Graphical view]
PANTHERiPTHR32182:SF0. PTHR32182:SF0. 1 hit.
PfamiPF02463. SMC_N. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
TIGRFAMsiTIGR00611. recf. 1 hit.
PROSITEiPS00617. RECF_1. 1 hit.
PS00618. RECF_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRECF_BACSU
AccessioniPrimary (citable) accession number: P05651
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: March 1, 1992
Last modified: September 7, 2016
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.