Reviewed, UniProtKB/Swiss-Prot P05651 (RECF_BACSU)

Names and origin

Protein names

Recommended name:
    DNA replication and repair protein recF

Gene names

Name:	recF
Ordered Locus Names:	BSU00040

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	370 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP. Is recruited to repair centers, foci that are the site of double-strand DNA break(s) after recN and recO; recruitment may depend on recO. HAMAP MF_00365
Subunit structure	Recruited to foci following DNA damage; probably interacts with recO. HAMAP MF_00365
Subcellular location	Cytoplasm › nucleoid. Note: Cytoplasmically located in untreated cells. Recruited to foci following treatment with DNA damaging agents; these foci are presumably the breaks themselves. They are almost always located within nucleoids. Ref.4
Sequence similarities	Belongs to the recF family.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication SOS response
Cellular component	Cytoplasm
Ligand	ATP-binding DNA-binding Nucleotide-binding
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA replication Inferred from electronic annotation. Source: HAMAP SOS response Inferred from electronic annotation. Source: HAMAP double-strand break repair Ref.4 Inferred from mutant phenotype. Source: UniProtKB
Cellular component	bacterial nucleoid Ref.4 Inferred from direct assay. Source: UniProtKB chromosome Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP single-stranded DNA binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 370	370	DNA replication and repair protein recF HAMAP MF_00365		PRO_0000196398
Regions
	Nucleotide binding	30 – 37	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	P05651-1 [UniParc]. Last modified March 1, 1992. Version 2. Checksum: 51ADB3DEA7CDE6E0	FASTA	370	42,304
	10 20 30 40 50 60 MYIQNLELTS YRNYDHAELQ FENKVNVIIG ENAQGKTNLM EAIYVLSMAK SHRTSNDKEL 70 80 90 100 110 120 IRWDKDYAKI EGRVMKQNGA IPMQLVISKK GKKGKVNHIE QQKLSQYVGA LNTIMFAPED 130 140 150 160 170 180 LNLVKGSPQV RRRFLDMEIG QVSPVYLHDL SLYQKILSQR NHFLKQLQTR KQTDRTMLDV 190 200 210 220 230 240 LTDQLVEVAA KVVVKRLQFT AQLEKWAQPI HAGISRGLEE LTLKYHTALD VSDPLDLSKI 250 260 270 280 290 300 GDSYQEAFSK LREKEIERGV TLSGPHRDDV LFYVNGRDVQ TYGSQGQQRT TALSLKLAEI 310 320 330 340 350 360 DLIHEEIGEY PILLLDDVLS ELDDYRQSHL LHTIQGRVQT FVTTTSVDGI DHETLRQAGM 370 FRVQNGALVK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure and function of the region of the replication origin of the Bacillus subtilis chromosome. III. Nucleotide sequence of some 10,000 base pairs in the origin region." Moriya S., Ogasawara N., Yoshikawa H. Nucleic Acids Res. 13:2251-2265(1985) [PubMed: 2987847] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Systematic sequencing of the 180 kilobase region of the Bacillus subtilis chromosome containing the replication origin." Ogasawara N., Nakai S., Yoshikawa H. DNA Res. 1:1-14(1994) [PubMed: 7584024] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[3]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[4]	"Visualization of DNA double-strand break repair in live bacteria reveals dynamic recruitment of Bacillus subtilis RecF, RecO and RecN proteins to distinct sites on the nucleoids." Kidane D., Sanchez H., Alonso J.C., Graumann P.L. Mol. Microbiol. 52:1627-1639(2004) [PubMed: 15186413] [Abstract] Cited for: SUBCELLULAR LOCATION, TEMPORAL ORDER OF DNA DOUBLE-STRAND BREAK RECRUITMENT. Strain: 168 / YB886 / BG214.
[5]	"Dynamic formation of RecA filaments at DNA double strand break repair centers in live cells." Kidane D., Graumann P.L. J. Cell Biol. 170:357-366(2005) [PubMed: 16061691] [Abstract] Cited for: RECRUITMENT OF RECA. Strain: 168 / YB886 / BG214.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X02369 Genomic DNA. Translation: CAA26220.1. Different initiation. D26185 Genomic DNA. Translation: BAA05240.1. AL009126 Genomic DNA. Translation: CAB11780.1.
PIR	D22930.
RefSeq	NP_387885.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	939454.
GenomeReviews	Gene locus BSU00040 in contig AL009126_GR.
KEGG	bsu:BSU00040.
NMPDR	fig|224308.1.peg.4.
Organism-specific databases
SubtiList	BG10068. recF. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	HBG645124.
OMA	NAQGKSN.
Family and domain databases
HAMAP	MF_00365. [Tree]
InterPro	IPR001238. DNA-binding_RecF. IPR018078. DNA-binding_RecF_CS. IPR003395. RecF/RecN/SMC_N. [Graphical view]
Pfam	PF02463. SMC_N. 1 hit. [Graphical view]
PROSITE	PS00617. RECF_1. 1 hit. PS00618. RECF_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RECF_BACSU

Accession

Primary (citable) accession number: P05651

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1988
Last sequence update:	March 1, 1992
Last modified:	December 15, 2009
This is version 75 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)