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Protein

DNA polymerase III subunit beta

Gene

dnaN

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped onto DNA, it slides freely (bidirectional and ATP-independent) along duplex DNA.

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

DNA-directed DNA polymerase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA replication

Enzyme and pathway databases

BioCyciBSUB:BSU00020-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase III subunit beta (EC:2.7.7.7)
Gene namesi
Name:dnaN
Synonyms:dnaG
Ordered Locus Names:BSU00020
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001054241 – 378DNA polymerase III subunit betaAdd BLAST378

Proteomic databases

PaxDbiP05649.
PRIDEiP05649.

Interactioni

Subunit structurei

Contains a core (composed of alpha, epsilon, and theta chains) that can repair short gaps created by nuclease in duplex DNA. For efficient replication of the long, single-stranded templates, pol III requires the auxiliary chains beta, gamma, and delta.

Protein-protein interaction databases

DIPiDIP-61105N.
IntActiP05649. 6 interactors.
MINTiMINT-8366930.
STRINGi224308.Bsubs1_010100000010.

Structurei

Secondary structure

1378
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 6Combined sources5
Helixi7 – 18Combined sources12
Helixi28 – 31Combined sources4
Beta strandi32 – 37Combined sources6
Beta strandi39 – 47Combined sources9
Beta strandi49 – 61Combined sources13
Beta strandi67 – 71Combined sources5
Beta strandi73 – 78Combined sources6
Helixi79 – 87Combined sources9
Beta strandi90 – 98Combined sources9
Helixi100 – 102Combined sources3
Beta strandi103 – 108Combined sources6
Beta strandi111 – 116Combined sources6
Helixi120 – 122Combined sources3
Beta strandi135 – 138Combined sources4
Helixi139 – 147Combined sources9
Helixi150 – 152Combined sources3
Helixi160 – 163Combined sources4
Beta strandi164 – 170Combined sources7
Beta strandi173 – 179Combined sources7
Beta strandi181 – 190Combined sources10
Beta strandi199 – 204Combined sources6
Helixi205 – 214Combined sources10
Beta strandi221 – 226Combined sources6
Beta strandi228 – 235Combined sources8
Beta strandi238 – 243Combined sources6
Turni253 – 255Combined sources3
Beta strandi261 – 267Combined sources7
Helixi268 – 278Combined sources11
Turni279 – 281Combined sources3
Beta strandi290 – 295Combined sources6
Turni296 – 298Combined sources3
Beta strandi299 – 306Combined sources8
Turni307 – 309Combined sources3
Beta strandi310 – 317Combined sources8
Beta strandi319 – 324Combined sources6
Beta strandi326 – 330Combined sources5
Helixi332 – 341Combined sources10
Beta strandi344 – 352Combined sources9
Turni353 – 355Combined sources3
Beta strandi358 – 361Combined sources4
Beta strandi367 – 372Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4TR6X-ray1.50A/B1-378[»]
ProteinModelPortaliP05649.
SMRiP05649.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

eggNOGiENOG4105CZ8. Bacteria.
COG0592. LUCA.
HOGENOMiHOG000071792.
InParanoidiP05649.
KOiK02338.
OMAiTHQIRLK.
PhylomeDBiP05649.

Family and domain databases

CDDicd00140. beta_clamp. 1 hit.
InterProiIPR001001. DNA_polIII_beta.
IPR022635. DNA_polIII_beta_C.
IPR022637. DNA_polIII_beta_cen.
IPR022634. DNA_polIII_beta_N.
[Graphical view]
PfamiPF00712. DNA_pol3_beta. 1 hit.
PF02767. DNA_pol3_beta_2. 1 hit.
PF02768. DNA_pol3_beta_3. 1 hit.
[Graphical view]
PIRSFiPIRSF000804. DNA_pol_III_b. 1 hit.
SMARTiSM00480. POL3Bc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00663. dnan. 1 hit.

Sequencei

Sequence statusi: Complete.

P05649-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFTIQKDRL VESVQDVLKA VSSRTTIPIL TGIKIVASDD GVSFTGSDSD
60 70 80 90 100
ISIESFIPKE EGDKEIVTIE QPGSIVLQAR FFSEIVKKLP MATVEIEVQN
110 120 130 140 150
QYLTIIRSGK AEFNLNGLDA DEYPHLPQIE EHHAIQIPTD LLKNLIRQTV
160 170 180 190 200
FAVSTSETRP ILTGVNWKVE QSELLCTATD SHRLALRKAK LDIPEDRSYN
210 220 230 240 250
VVIPGKSLTE LSKILDDNQE LVDIVITETQ VLFKAKNVLF FSRLLDGNYP
260 270 280 290 300
DTTSLIPQDS KTEIIVNTKE FLQAIDRASL LAREGRNNVV KLSAKPAESI
310 320 330 340 350
EISSNSPEIG KVVEAIVADQ IEGEELNISF SPKYMLDALK VLEGAEIRVS
360 370
FTGAMRPFLI RTPNDETIVQ LILPVRTY
Length:378
Mass (Da):42,103
Last modified:November 1, 1988 - v1
Checksum:iC1F040B5D53026C8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02369 Genomic DNA. Translation: CAA26218.1.
D26185 Genomic DNA. Translation: BAA05238.1.
AL009126 Genomic DNA. Translation: CAB11778.1.
X12779 Genomic DNA. Translation: CAA31271.1.
PIRiB22930.
RefSeqiNP_387883.1. NC_000964.3.
WP_003242509.1. NZ_JNCM01000034.1.

Genome annotation databases

EnsemblBacteriaiCAB11778; CAB11778; BSU00020.
GeneIDi939970.
KEGGibsu:BSU00020.
PATRICi18971451. VBIBacSub10457_0002.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02369 Genomic DNA. Translation: CAA26218.1.
D26185 Genomic DNA. Translation: BAA05238.1.
AL009126 Genomic DNA. Translation: CAB11778.1.
X12779 Genomic DNA. Translation: CAA31271.1.
PIRiB22930.
RefSeqiNP_387883.1. NC_000964.3.
WP_003242509.1. NZ_JNCM01000034.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4TR6X-ray1.50A/B1-378[»]
ProteinModelPortaliP05649.
SMRiP05649.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-61105N.
IntActiP05649. 6 interactors.
MINTiMINT-8366930.
STRINGi224308.Bsubs1_010100000010.

Proteomic databases

PaxDbiP05649.
PRIDEiP05649.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB11778; CAB11778; BSU00020.
GeneIDi939970.
KEGGibsu:BSU00020.
PATRICi18971451. VBIBacSub10457_0002.

Phylogenomic databases

eggNOGiENOG4105CZ8. Bacteria.
COG0592. LUCA.
HOGENOMiHOG000071792.
InParanoidiP05649.
KOiK02338.
OMAiTHQIRLK.
PhylomeDBiP05649.

Enzyme and pathway databases

BioCyciBSUB:BSU00020-MONOMER.

Family and domain databases

CDDicd00140. beta_clamp. 1 hit.
InterProiIPR001001. DNA_polIII_beta.
IPR022635. DNA_polIII_beta_C.
IPR022637. DNA_polIII_beta_cen.
IPR022634. DNA_polIII_beta_N.
[Graphical view]
PfamiPF00712. DNA_pol3_beta. 1 hit.
PF02767. DNA_pol3_beta_2. 1 hit.
PF02768. DNA_pol3_beta_3. 1 hit.
[Graphical view]
PIRSFiPIRSF000804. DNA_pol_III_b. 1 hit.
SMARTiSM00480. POL3Bc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00663. dnan. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiDPO3B_BACSU
AccessioniPrimary (citable) accession number: P05649
Secondary accession number(s): P11571
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: November 1, 1988
Last modified: November 2, 2016
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.