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Protein

Beta sliding clamp

Gene

dnaN

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Confers DNA tethering and processivity to DNA polymerases and other proteins. Acts as a clamp, forming a ring around DNA (a reaction catalyzed by the clamp-loading complex) which diffuses in an ATP-independent manner freely and bidirectionally along dsDNA. Initially characterized for its ability to contact the catalytic subunit of DNA polymerase III (Pol III), a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria; Pol III exhibits 3'-5' exonuclease proofreading activity. The beta chain is required for initiation of replication as well as for processivity of DNA replication.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, DNA-directed DNA polymerase, Nucleotidyltransferase, Transferase
Biological processDNA replication

Enzyme and pathway databases

BioCyciBSUB:BSU00020-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta sliding clamp
Short name:
Beta clamp
Short name:
Sliding clamp
Alternative name(s):
Beta-clamp processivity factor
DNA polymerase III beta sliding clamp subunit
DNA polymerase III subunit beta
Gene namesi
Name:dnaN
Synonyms:dnaG
Ordered Locus Names:BSU00020
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001054241 – 378Beta sliding clampAdd BLAST378

Proteomic databases

PaxDbiP05649.
PRIDEiP05649.

Interactioni

Subunit structurei

Forms a ring-shaped head-to-tail homodimer (PubMed:25170813) around DNA which binds and tethers DNA polymerases and other proteins to the DNA. The DNA replisome complex has a single clamp-loading complex (3 tau and 1 each of delta, delta', psi and chi subunits) which binds 3 Pol III cores (1 core on the leading strand and 2 on the lagging strand) each with a beta sliding clamp dimer. Additional proteins in the replisome are other copies of gamma, psi and chi, Ssb, DNA helicase and RNA primase.By similarity1 Publication

Protein-protein interaction databases

DIPiDIP-61105N.
IntActiP05649. 6 interactors.
MINTiMINT-8366930.
STRINGi224308.Bsubs1_010100000010.

Structurei

Secondary structure

1378
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 6Combined sources5
Helixi7 – 18Combined sources12
Helixi28 – 31Combined sources4
Beta strandi32 – 37Combined sources6
Beta strandi39 – 47Combined sources9
Beta strandi49 – 61Combined sources13
Beta strandi67 – 71Combined sources5
Beta strandi73 – 78Combined sources6
Helixi79 – 87Combined sources9
Beta strandi90 – 98Combined sources9
Helixi100 – 102Combined sources3
Beta strandi103 – 108Combined sources6
Beta strandi111 – 116Combined sources6
Helixi120 – 122Combined sources3
Beta strandi135 – 138Combined sources4
Helixi139 – 147Combined sources9
Helixi150 – 152Combined sources3
Helixi160 – 163Combined sources4
Beta strandi164 – 170Combined sources7
Beta strandi173 – 179Combined sources7
Beta strandi181 – 190Combined sources10
Beta strandi199 – 204Combined sources6
Helixi205 – 214Combined sources10
Beta strandi221 – 226Combined sources6
Beta strandi228 – 235Combined sources8
Beta strandi238 – 243Combined sources6
Turni253 – 255Combined sources3
Beta strandi261 – 267Combined sources7
Helixi268 – 278Combined sources11
Turni279 – 281Combined sources3
Beta strandi290 – 295Combined sources6
Turni296 – 298Combined sources3
Beta strandi299 – 306Combined sources8
Turni307 – 309Combined sources3
Beta strandi310 – 317Combined sources8
Beta strandi319 – 324Combined sources6
Beta strandi326 – 330Combined sources5
Helixi332 – 341Combined sources10
Beta strandi344 – 352Combined sources9
Turni353 – 355Combined sources3
Beta strandi358 – 361Combined sources4
Beta strandi367 – 372Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4TR6X-ray1.50A/B1-378[»]
ProteinModelPortaliP05649.
SMRiP05649.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the beta sliding clamp family.Curated

Phylogenomic databases

eggNOGiENOG4105CZ8. Bacteria.
COG0592. LUCA.
HOGENOMiHOG000071792.
InParanoidiP05649.
KOiK02338.
OMAiNYEAVIP.
PhylomeDBiP05649.

Family and domain databases

CDDicd00140. beta_clamp. 1 hit.
InterProiView protein in InterPro
IPR001001. DNA_polIII_beta.
IPR022635. DNA_polIII_beta_C.
IPR022637. DNA_polIII_beta_cen.
IPR022634. DNA_polIII_beta_N.
PANTHERiPTHR30478:SF1. PTHR30478:SF1. 1 hit.
PfamiView protein in Pfam
PF00712. DNA_pol3_beta. 1 hit.
PF02767. DNA_pol3_beta_2. 1 hit.
PF02768. DNA_pol3_beta_3. 1 hit.
PIRSFiPIRSF000804. DNA_pol_III_b. 1 hit.
SMARTiView protein in SMART
SM00480. POL3Bc. 1 hit.
TIGRFAMsiTIGR00663. dnan. 1 hit.

Sequencei

Sequence statusi: Complete.

P05649-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFTIQKDRL VESVQDVLKA VSSRTTIPIL TGIKIVASDD GVSFTGSDSD
60 70 80 90 100
ISIESFIPKE EGDKEIVTIE QPGSIVLQAR FFSEIVKKLP MATVEIEVQN
110 120 130 140 150
QYLTIIRSGK AEFNLNGLDA DEYPHLPQIE EHHAIQIPTD LLKNLIRQTV
160 170 180 190 200
FAVSTSETRP ILTGVNWKVE QSELLCTATD SHRLALRKAK LDIPEDRSYN
210 220 230 240 250
VVIPGKSLTE LSKILDDNQE LVDIVITETQ VLFKAKNVLF FSRLLDGNYP
260 270 280 290 300
DTTSLIPQDS KTEIIVNTKE FLQAIDRASL LAREGRNNVV KLSAKPAESI
310 320 330 340 350
EISSNSPEIG KVVEAIVADQ IEGEELNISF SPKYMLDALK VLEGAEIRVS
360 370
FTGAMRPFLI RTPNDETIVQ LILPVRTY
Length:378
Mass (Da):42,103
Last modified:November 1, 1988 - v1
Checksum:iC1F040B5D53026C8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02369 Genomic DNA. Translation: CAA26218.1.
D26185 Genomic DNA. Translation: BAA05238.1.
AL009126 Genomic DNA. Translation: CAB11778.1.
X12779 Genomic DNA. Translation: CAA31271.1.
PIRiB22930.
RefSeqiNP_387883.1. NC_000964.3.
WP_003242509.1. NZ_JNCM01000034.1.

Genome annotation databases

EnsemblBacteriaiCAB11778; CAB11778; BSU00020.
GeneIDi939970.
KEGGibsu:BSU00020.
PATRICifig|224308.179.peg.2.

Similar proteinsi

Entry informationi

Entry nameiDPO3B_BACSU
AccessioniPrimary (citable) accession number: P05649
Secondary accession number(s): P11571
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: November 1, 1988
Last modified: September 27, 2017
This is version 124 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families