DNA polymerase III subunit beta - Bacillus subtilis (strain 168)

Contribute

Send feedback

Read comments (?) or add your own

P05649 (DPO3B_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 97. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
DNA polymerase III subunit beta
EC=2.7.7.7

Gene names

Name:	dnaN
Synonyms:	dnaG
Ordered Locus Names:	BSU00020

Organism

Bacillus subtilis (strain 168) [Reference proteome] [HAMAP]

Taxonomic identifier

224308 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus ›

Protein attributes

Sequence length	378 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped onto DNA, it slides freely (bidirectional and ATP-independent) along duplex DNA.
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
Subunit structure	Contains a core (composed of alpha, epsilon, and theta chains) that can repair short gaps created by nuclease in duplex DNA. For efficient replication of the long, single-stranded templates, pol III requires the auxiliary chains beta, gamma, and delta.
Subcellular location	Cytoplasm By similarity.

Ontologies

Keywords
Biological process	DNA replication
Cellular component	Cytoplasm
Molecular function	DNA-directed DNA polymerase Nucleotidyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	DNA polymerase III complex Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	3'-5' exonuclease activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 378

378

DNA polymerase III subunit beta

PRO_0000105424

Sequences

Sequence

Length

Mass (Da)

Tools

P05649 [UniParc].

Last modified November 1, 1988. Version 1.
Checksum: C1F040B5D53026C8
Show »

FASTA

378

42,103

        10         20         30         40         50         60 
MKFTIQKDRL VESVQDVLKA VSSRTTIPIL TGIKIVASDD GVSFTGSDSD ISIESFIPKE 

        70         80         90        100        110        120 
EGDKEIVTIE QPGSIVLQAR FFSEIVKKLP MATVEIEVQN QYLTIIRSGK AEFNLNGLDA 

       130        140        150        160        170        180 
DEYPHLPQIE EHHAIQIPTD LLKNLIRQTV FAVSTSETRP ILTGVNWKVE QSELLCTATD 

       190        200        210        220        230        240 
SHRLALRKAK LDIPEDRSYN VVIPGKSLTE LSKILDDNQE LVDIVITETQ VLFKAKNVLF 

       250        260        270        280        290        300 
FSRLLDGNYP DTTSLIPQDS KTEIIVNTKE FLQAIDRASL LAREGRNNVV KLSAKPAESI 

       310        320        330        340        350        360 
EISSNSPEIG KVVEAIVADQ IEGEELNISF SPKYMLDALK VLEGAEIRVS FTGAMRPFLI 

       370 
RTPNDETIVQ LILPVRTY

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure and function of the region of the replication origin of the Bacillus subtilis chromosome. III. Nucleotide sequence of some 10,000 base pairs in the origin region." Moriya S., Ogasawara N., Yoshikawa H. Nucleic Acids Res. 13:2251-2265(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Systematic sequencing of the 180 kilobase region of the Bacillus subtilis chromosome containing the replication origin." Ogasawara N., Nakai S., Yoshikawa H. DNA Res. 1:1-14(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[3]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. Danchin A. Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[4]	"Regulation of initiation of the chromosomal replication by DnaA-boxes in the origin region of the Bacillus subtilis chromosome." Moriya S., Fukuoka T., Ogasawara N., Yoshikawa H. EMBO J. 7:2911-2917(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-29. Strain: 168 / PSL1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X02369 Genomic DNA. Translation: CAA26218.1. D26185 Genomic DNA. Translation: BAA05238.1. AL009126 Genomic DNA. Translation: CAB11778.1. X12779 Genomic DNA. Translation: CAA31271.1.
PIR	B22930.
RefSeq	NP_387883.1. NC_000964.3.
3D structure databases
ProteinModelPortal	P05649.
SMR	P05649. Positions 1-377.
ModBase	Search...
Protein-protein interaction databases
IntAct	P05649. 5 interactions.
STRING	224308.BSU00020.
Proteomic databases
PaxDb	P05649.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAB11778; CAB11778; BSU00020.
GeneID	939970.
KEGG	bsu:BSU00020.
PATRIC	18971451. VBIBacSub10457_0002.
Organism-specific databases
GenoList	BSU00020. [Micado]
Phylogenomic databases
eggNOG	COG0592.
HOGENOM	HOG000071792.
KO	K02338.
OMA	DYNRVIP.
ProtClustDB	PRK05643.
Enzyme and pathway databases
BioCyc	BSUB:BSU00020-MONOMER.
Family and domain databases
InterPro	IPR001001. DNA_polIII_beta. IPR022635. DNA_polIII_beta_C. IPR022637. DNA_polIII_beta_cen. IPR022634. DNA_polIII_beta_N. [Graphical view]
Pfam	PF00712. DNA_pol3_beta. 1 hit. PF02767. DNA_pol3_beta_2. 1 hit. PF02768. DNA_pol3_beta_3. 1 hit. [Graphical view]
PIRSF	PIRSF000804. DNA_pol_III_b. 1 hit.
SMART	SM00480. POL3Bc. 1 hit. [Graphical view]
TIGRFAMs	TIGR00663. dnan. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DPO3B_BACSU

Accession

Primary (citable) accession number: P05649
Secondary accession number(s): P11571

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1988
Last sequence update:	November 1, 1988
Last modified:	May 1, 2013
This is version 97 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents