Reviewed, UniProtKB/Swiss-Prot P05649 (DPO3B_BACSU)

Names and origin

Protein names

Recommended name:
    DNA polymerase III subunit beta
    EC=2.7.7.7

Gene names

Name:	dnaN
Synonyms:	dnaG
Ordered Locus Names:	BSU00020

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	378 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped onto DNA, it slides freely (bidirectional and ATP-independent) along duplex DNA.
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
Subunit structure	Contains a core (composed of alpha, epsilon, and theta chains) that can repair short gaps created by nuclease in duplex DNA. For efficient replication of the long, single-stranded templates, pol III requires the auxiliary chains beta, gamma, and delta.
Subcellular location	Cytoplasm By similarity.

Ontologies

Keywords
Biological process	DNA replication
Cellular component	Cytoplasm
Molecular function	DNA-directed DNA polymerase Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 378	378	DNA polymerase III subunit beta		PRO_0000105424

Sequences

	Sequence		Length	Mass (Da)
	P05649-1 [UniParc]. Last modified November 1, 1988. Version 1. Checksum: C1F040B5D53026C8	FASTA	378	42,103
	10 20 30 40 50 60 MKFTIQKDRL VESVQDVLKA VSSRTTIPIL TGIKIVASDD GVSFTGSDSD ISIESFIPKE 70 80 90 100 110 120 EGDKEIVTIE QPGSIVLQAR FFSEIVKKLP MATVEIEVQN QYLTIIRSGK AEFNLNGLDA 130 140 150 160 170 180 DEYPHLPQIE EHHAIQIPTD LLKNLIRQTV FAVSTSETRP ILTGVNWKVE QSELLCTATD 190 200 210 220 230 240 SHRLALRKAK LDIPEDRSYN VVIPGKSLTE LSKILDDNQE LVDIVITETQ VLFKAKNVLF 250 260 270 280 290 300 FSRLLDGNYP DTTSLIPQDS KTEIIVNTKE FLQAIDRASL LAREGRNNVV KLSAKPAESI 310 320 330 340 350 360 EISSNSPEIG KVVEAIVADQ IEGEELNISF SPKYMLDALK VLEGAEIRVS FTGAMRPFLI 370 RTPNDETIVQ LILPVRTY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure and function of the region of the replication origin of the Bacillus subtilis chromosome. III. Nucleotide sequence of some 10,000 base pairs in the origin region." Moriya S., Ogasawara N., Yoshikawa H. Nucleic Acids Res. 13:2251-2265(1985) [PubMed: 2987847] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Systematic sequencing of the 180 kilobase region of the Bacillus subtilis chromosome containing the replication origin." Ogasawara N., Nakai S., Yoshikawa H. DNA Res. 1:1-14(1994) [PubMed: 7584024] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[3]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[4]	"Regulation of initiation of the chromosomal replication by DnaA-boxes in the origin region of the Bacillus subtilis chromosome." Moriya S., Fukuoka T., Ogasawara N., Yoshikawa H. EMBO J. 7:2911-2917(1988) [PubMed: 2846289] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-29. Strain: 168 / PSL1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X02369 Genomic DNA. Translation: CAA26218.1. D26185 Genomic DNA. Translation: BAA05238.1. AL009126 Genomic DNA. Translation: CAB11778.1. X12779 Genomic DNA. Translation: CAA31271.1.
PIR	B22930.
RefSeq	NP_387883.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	939970.
GenomeReviews	Gene locus BSU00020 in contig AL009126_GR.
KEGG	bsu:BSU00020.
NMPDR	fig|224308.1.peg.2.
Organism-specific databases
SubtiList	BG10066. dnaN. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	HBG586514.
OMA	IGFNARY.
Enzyme and pathway databases
BRENDA	2.7.7.7. 150.
Family and domain databases
InterPro	IPR001001. DNA_polIII_beta. [Graphical view]
Gene3D	G3DSA:3.10.150.10. DNA_polIII_beta. 3 hits.
Pfam	PF00712. DNA_pol3_beta. 1 hit. PF02767. DNA_pol3_beta_2. 1 hit. PF02768. DNA_pol3_beta_3. 1 hit. [Graphical view]
SMART	SM00480. POL3Bc. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DPO3B_BACSU

Accession

Primary (citable) accession number: P05649
Secondary accession number(s): P11571

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1988
Last sequence update:	November 1, 1988
Last modified:	December 15, 2009
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)