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P05648 (DNAA_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Chromosomal replication initiator protein DnaA
Gene names
Name:dnaA
Synonyms:dnaH
Ordered Locus Names:BSU00010
OrganismBacillus subtilis
Taxonomic identifier1423 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length446 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box): 5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phospholipids. DnaA can inhibit its own gene expression as well as that of other genes. HAMAP MF_00377

Subunit structure

Some 20 DnaA protein molecules bind their sites in oriC.

Subcellular location

Cytoplasm By similarity HAMAP MF_00377.

Sequence similarities

Belongs to the DnaA family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

sojP375222EBI-2014722,EBI-2014674

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 446446Chromosomal replication initiator protein DnaA HAMAP MF_00377
PRO_0000114133

Regions

Nucleotide binding151 – 1588ATP Potential

Sequences

Sequence LengthMass (Da)Tools
P05648 [UniParc].

Last modified November 1, 1988. Version 1.
Checksum: 666799A0A183C992

FASTA44650,859
        10         20         30         40         50         60 
MENILDLWNQ ALAQIEKKLS KPSFETWMKS TKAHSLQGDT LTITAPNEFA RDWLESRYLH 

        70         80         90        100        110        120 
LIADTIYELT GEELSIKFVI PQNQDVEDFM PKPQVKKAVK EDTSDFPQNM LNPKYTFDTF 

       130        140        150        160        170        180 
VIGSGNRFAH AASLAVAEAP AKAYNPLFIY GGVGLGKTHL MHAIGHYVID HNPSAKVVYL 

       190        200        210        220        230        240 
SSEKFTNEFI NSIRDNKAVD FRNRYRNVDV LLIDDIQFLA GKEQTQEEFF HTFNTLHEES 

       250        260        270        280        290        300 
KQIVISSDRP PKEIPTLEDR LRSRFEWGLI TDITPPDLET RIAILRKKAK AEGLDIPNEV 

       310        320        330        340        350        360 
MLYIANQIDS NIRELEGALI RVVAYSSLIN KDINADLAAE ALKDIIPSSK PKVITIKEIQ 

       370        380        390        400        410        420 
RVVGQQFNIK LEDFKAKKRT KSVAFPRQIA MYLSREMTDS SLPKIGEEFG GRDHTTVIHA 

       430        440 
HEKISKLLAD DEQLQQHVKE IKEQLK

« Hide

References

« Hide 'large scale' references
[1]"Structure and function of the region of the replication origin of the Bacillus subtilis chromosome. III. Nucleotide sequence of some 10,000 base pairs in the origin region."
Moriya S., Ogasawara N., Yoshikawa H.
Nucleic Acids Res. 13:2251-2265(1985) [PubMed: 2987847] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Systematic sequencing of the 180 kilobase region of the Bacillus subtilis chromosome containing the replication origin."
Ogasawara N., Nakai S., Yoshikawa H.
DNA Res. 1:1-14(1994) [PubMed: 7584024] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168.
[3]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed: 9384377] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[4]"Regulation of initiation of the chromosomal replication by DnaA-boxes in the origin region of the Bacillus subtilis chromosome."
Moriya S., Fukuoka T., Ogasawara N., Yoshikawa H.
EMBO J. 7:2911-2917(1988) [PubMed: 2846289] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-6 AND 441-446.
Strain: 168 / PSL1.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X02369 Genomic DNA. Translation: CAA26217.1.
D26185 Genomic DNA. Translation: BAA05237.1.
AL009126 Genomic DNA. Translation: CAB11777.1.
X12778 Genomic DNA. Translation: CAA31269.1.
X12779 Genomic DNA. Translation: CAA31270.1.
PIRIQBSOC. A22930.
RefSeqNP_387882.1. NC_000964.3.

3D structure databases

ProteinModelPortalP05648.
SMRP05648. Positions 5-83, 109-446.
ModBaseSearch...

Protein-protein interaction databases

IntActP05648. 8 interactions.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBBACT00000003301; EBBACP00000003301; EBBACG00000003294.
GeneID939978.
GenomeReviewsGene locus BSU00010 in contig AL009126_GR.
KEGGbsu:BSU00010.
NMPDRfig|224308.1.peg.1.
PATRIC18971449. VBIBacSub10457_0001.

Organism-specific databases

GenoListBSU00010. [Micado]

Phylogenomic databases

GeneTreeEBGT00050000000588.
HOGENOMHBG695666.
OMAIQRVVGQ.
PhylomeDBP05648.
ProtClustDBPRK00149.

Enzyme and pathway databases

BioCycBSUB:BSU00010-MONOMER.

Family and domain databases

HAMAPMF_00377. DnaA_bact.
[Tree]
InterProIPR003593. ATPase_AAA+_core.
IPR001957. Chromosome_initiator_DnaA.
IPR020591. Chromosome_initiator_DnaA-like.
IPR018312. Chromosome_initiator_DnaA_CS.
IPR013159. DnaA_C.
IPR013317. DnaA_Hda.
IPR010921. Trp_repressor/repl_initiator.
[Graphical view]
Gene3DG3DSA:1.10.1750.10. DnaA_C. 1 hit.
KOK02313.
PfamPF00308. Bac_DnaA. 1 hit.
PF08299. Bac_DnaA_C. 1 hit.
[Graphical view]
PRINTSPR00051. DNAA.
SMARTSM00382. AAA. 1 hit.
SM00760. Bac_DnaA_C. 1 hit.
[Graphical view]
SUPFAMSSF48295. Trp_repress_rep. 1 hit.
TIGRFAMsTIGR00362. DnaA. 1 hit.
PROSITEPS01008. DNAA. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDNAA_BACSU
AccessionPrimary (citable) accession number: P05648
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: November 1, 1988
Last modified: January 25, 2012
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents