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Protein

Chromosomal replication initiator protein DnaA

Gene

dnaA

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box): 5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phospholipids. DnaA can inhibit its own gene expression as well as that of other genes.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi151 – 158ATPSequence analysis8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • DNA replication origin binding Source: GO_Central
  • identical protein binding Source: IntAct

GO - Biological processi

  • DNA replication initiation Source: CACAO
  • regulation of DNA replication Source: InterPro

Keywordsi

Molecular functionDNA-binding
Biological processDNA replication
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBSUB:BSU00010-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Chromosomal replication initiator protein DnaA
Gene namesi
Name:dnaA
Synonyms:dnaH
Ordered Locus Names:BSU00010
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001141331 – 446Chromosomal replication initiator protein DnaAAdd BLAST446

Proteomic databases

PaxDbiP05648

Interactioni

Subunit structurei

Some 20 DnaA protein molecules bind their sites in oriC.

Binary interactionsi

Show more details

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

DIPiDIP-41940N
IntActiP05648, 7 interactors
STRINGi224308.Bsubs1_010100000005

Structurei

Secondary structure

1446
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi1 – 16Combined sources16
Helixi21 – 27Combined sources7
Turni28 – 30Combined sources3
Beta strandi32 – 37Combined sources6
Beta strandi40 – 44Combined sources5
Helixi48 – 57Combined sources10
Helixi59 – 70Combined sources12
Beta strandi75 – 79Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4TPSX-ray1.65B/D1-82[»]
ProteinModelPortaliP05648
SMRiP05648
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DnaA family.Curated

Phylogenomic databases

eggNOGiENOG4105CI4 Bacteria
COG0593 LUCA
HOGENOMiHOG000235658
InParanoidiP05648
KOiK02313
OMAiREFNPLF
PhylomeDBiP05648

Family and domain databases

CDDicd06571 Bac_DnaA_C, 1 hit
Gene3Di1.10.1750.10, 1 hit
3.30.300.180, 1 hit
HAMAPiMF_00377 DnaA_bact, 1 hit
InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR001957 Chromosome_initiator_DnaA
IPR020591 Chromosome_initiator_DnaA-like
IPR018312 Chromosome_initiator_DnaA_CS
IPR013317 DnaA
IPR013159 DnaA_C
IPR024633 DnaA_N_dom
IPR038454 DnaA_N_sf
IPR027417 P-loop_NTPase
IPR010921 Trp_repressor/repl_initiator
PANTHERiPTHR30050:SF2 PTHR30050:SF2, 1 hit
PfamiView protein in Pfam
PF00308 Bac_DnaA, 1 hit
PF08299 Bac_DnaA_C, 1 hit
PF11638 DnaA_N, 1 hit
PRINTSiPR00051 DNAA
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SM00760 Bac_DnaA_C, 1 hit
SUPFAMiSSF48295 SSF48295, 1 hit
SSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00362 DnaA, 1 hit
PROSITEiView protein in PROSITE
PS01008 DNAA, 1 hit

Sequencei

Sequence statusi: Complete.

P05648-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MENILDLWNQ ALAQIEKKLS KPSFETWMKS TKAHSLQGDT LTITAPNEFA
60 70 80 90 100
RDWLESRYLH LIADTIYELT GEELSIKFVI PQNQDVEDFM PKPQVKKAVK
110 120 130 140 150
EDTSDFPQNM LNPKYTFDTF VIGSGNRFAH AASLAVAEAP AKAYNPLFIY
160 170 180 190 200
GGVGLGKTHL MHAIGHYVID HNPSAKVVYL SSEKFTNEFI NSIRDNKAVD
210 220 230 240 250
FRNRYRNVDV LLIDDIQFLA GKEQTQEEFF HTFNTLHEES KQIVISSDRP
260 270 280 290 300
PKEIPTLEDR LRSRFEWGLI TDITPPDLET RIAILRKKAK AEGLDIPNEV
310 320 330 340 350
MLYIANQIDS NIRELEGALI RVVAYSSLIN KDINADLAAE ALKDIIPSSK
360 370 380 390 400
PKVITIKEIQ RVVGQQFNIK LEDFKAKKRT KSVAFPRQIA MYLSREMTDS
410 420 430 440
SLPKIGEEFG GRDHTTVIHA HEKISKLLAD DEQLQQHVKE IKEQLK
Length:446
Mass (Da):50,859
Last modified:November 1, 1988 - v1
Checksum:i666799A0A183C992
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02369 Genomic DNA Translation: CAA26217.1
D26185 Genomic DNA Translation: BAA05237.1
AL009126 Genomic DNA Translation: CAB11777.1
X12778 Genomic DNA Translation: CAA31269.1
X12779 Genomic DNA Translation: CAA31270.1
PIRiA22930 IQBSOC
RefSeqiNP_387882.1, NC_000964.3
WP_003242674.1, NZ_JNCM01000034.1

Genome annotation databases

EnsemblBacteriaiCAB11777; CAB11777; BSU00010
GeneIDi939978
KEGGibsu:BSU00010
PATRICifig|224308.179.peg.1

Similar proteinsi

Entry informationi

Entry nameiDNAA_BACSU
AccessioniPrimary (citable) accession number: P05648
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: November 1, 1988
Last modified: March 28, 2018
This is version 141 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health