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Protein

Chromosomal replication initiator protein DnaA

Gene

dnaA

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box): 5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phospholipids. DnaA can inhibit its own gene expression as well as that of other genes.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi151 – 1588ATPSequence analysis

GO - Molecular functioni

GO - Biological processi

  • DNA replication initiation Source: CACAO
  • regulation of DNA replication Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBSUB:BSU00010-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Chromosomal replication initiator protein DnaA
Gene namesi
Name:dnaA
Synonyms:dnaH
Ordered Locus Names:BSU00010
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 446446Chromosomal replication initiator protein DnaAPRO_0000114133Add
BLAST

Proteomic databases

PaxDbiP05648.

Interactioni

Subunit structurei

Some 20 DnaA protein molecules bind their sites in oriC.

Binary interactionsi

WithEntry#Exp.IntActNotes
sojP375222EBI-2014722,EBI-2014674

Protein-protein interaction databases

DIPiDIP-41940N.
IntActiP05648. 7 interactions.
MINTiMINT-188900.
STRINGi224308.Bsubs1_010100000005.

Structurei

Secondary structure

446
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi1 – 1616Combined sources
Helixi21 – 277Combined sources
Turni28 – 303Combined sources
Beta strandi32 – 376Combined sources
Beta strandi40 – 445Combined sources
Helixi48 – 5710Combined sources
Helixi59 – 7012Combined sources
Beta strandi75 – 795Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4TPSX-ray1.65B/D1-82[»]
ProteinModelPortaliP05648.
SMRiP05648. Positions 5-83, 109-446.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DnaA family.Curated

Phylogenomic databases

eggNOGiENOG4105CI4. Bacteria.
COG0593. LUCA.
HOGENOMiHOG000235658.
InParanoidiP05648.
KOiK02313.
OMAiSHACDKI.
OrthoDBiEOG689HR1.
PhylomeDBiP05648.

Family and domain databases

Gene3Di1.10.1750.10. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00377. DnaA_bact.
InterProiIPR003593. AAA+_ATPase.
IPR001957. Chromosome_initiator_DnaA.
IPR020591. Chromosome_initiator_DnaA-like.
IPR018312. Chromosome_initiator_DnaA_CS.
IPR013317. DnaA.
IPR013159. DnaA_C.
IPR024633. DnaA_N_dom.
IPR027417. P-loop_NTPase.
IPR010921. Trp_repressor/repl_initiator.
[Graphical view]
PfamiPF00308. Bac_DnaA. 1 hit.
PF08299. Bac_DnaA_C. 1 hit.
PF11638. DnaA_N. 1 hit.
[Graphical view]
PRINTSiPR00051. DNAA.
SMARTiSM00382. AAA. 1 hit.
SM00760. Bac_DnaA_C. 1 hit.
[Graphical view]
SUPFAMiSSF48295. SSF48295. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00362. DnaA. 1 hit.
PROSITEiPS01008. DNAA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P05648-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MENILDLWNQ ALAQIEKKLS KPSFETWMKS TKAHSLQGDT LTITAPNEFA
60 70 80 90 100
RDWLESRYLH LIADTIYELT GEELSIKFVI PQNQDVEDFM PKPQVKKAVK
110 120 130 140 150
EDTSDFPQNM LNPKYTFDTF VIGSGNRFAH AASLAVAEAP AKAYNPLFIY
160 170 180 190 200
GGVGLGKTHL MHAIGHYVID HNPSAKVVYL SSEKFTNEFI NSIRDNKAVD
210 220 230 240 250
FRNRYRNVDV LLIDDIQFLA GKEQTQEEFF HTFNTLHEES KQIVISSDRP
260 270 280 290 300
PKEIPTLEDR LRSRFEWGLI TDITPPDLET RIAILRKKAK AEGLDIPNEV
310 320 330 340 350
MLYIANQIDS NIRELEGALI RVVAYSSLIN KDINADLAAE ALKDIIPSSK
360 370 380 390 400
PKVITIKEIQ RVVGQQFNIK LEDFKAKKRT KSVAFPRQIA MYLSREMTDS
410 420 430 440
SLPKIGEEFG GRDHTTVIHA HEKISKLLAD DEQLQQHVKE IKEQLK
Length:446
Mass (Da):50,859
Last modified:November 1, 1988 - v1
Checksum:i666799A0A183C992
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02369 Genomic DNA. Translation: CAA26217.1.
D26185 Genomic DNA. Translation: BAA05237.1.
AL009126 Genomic DNA. Translation: CAB11777.1.
X12778 Genomic DNA. Translation: CAA31269.1.
X12779 Genomic DNA. Translation: CAA31270.1.
PIRiA22930. IQBSOC.
RefSeqiNP_387882.1. NC_000964.3.
WP_003242674.1. NZ_JNCM01000034.1.

Genome annotation databases

EnsemblBacteriaiCAB11777; CAB11777; BSU00010.
GeneIDi11241639.
939978.
KEGGibsu:BSU00010.
PATRICi18971449. VBIBacSub10457_0001.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02369 Genomic DNA. Translation: CAA26217.1.
D26185 Genomic DNA. Translation: BAA05237.1.
AL009126 Genomic DNA. Translation: CAB11777.1.
X12778 Genomic DNA. Translation: CAA31269.1.
X12779 Genomic DNA. Translation: CAA31270.1.
PIRiA22930. IQBSOC.
RefSeqiNP_387882.1. NC_000964.3.
WP_003242674.1. NZ_JNCM01000034.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4TPSX-ray1.65B/D1-82[»]
ProteinModelPortaliP05648.
SMRiP05648. Positions 5-83, 109-446.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-41940N.
IntActiP05648. 7 interactions.
MINTiMINT-188900.
STRINGi224308.Bsubs1_010100000005.

Proteomic databases

PaxDbiP05648.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB11777; CAB11777; BSU00010.
GeneIDi11241639.
939978.
KEGGibsu:BSU00010.
PATRICi18971449. VBIBacSub10457_0001.

Phylogenomic databases

eggNOGiENOG4105CI4. Bacteria.
COG0593. LUCA.
HOGENOMiHOG000235658.
InParanoidiP05648.
KOiK02313.
OMAiSHACDKI.
OrthoDBiEOG689HR1.
PhylomeDBiP05648.

Enzyme and pathway databases

BioCyciBSUB:BSU00010-MONOMER.

Family and domain databases

Gene3Di1.10.1750.10. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00377. DnaA_bact.
InterProiIPR003593. AAA+_ATPase.
IPR001957. Chromosome_initiator_DnaA.
IPR020591. Chromosome_initiator_DnaA-like.
IPR018312. Chromosome_initiator_DnaA_CS.
IPR013317. DnaA.
IPR013159. DnaA_C.
IPR024633. DnaA_N_dom.
IPR027417. P-loop_NTPase.
IPR010921. Trp_repressor/repl_initiator.
[Graphical view]
PfamiPF00308. Bac_DnaA. 1 hit.
PF08299. Bac_DnaA_C. 1 hit.
PF11638. DnaA_N. 1 hit.
[Graphical view]
PRINTSiPR00051. DNAA.
SMARTiSM00382. AAA. 1 hit.
SM00760. Bac_DnaA_C. 1 hit.
[Graphical view]
SUPFAMiSSF48295. SSF48295. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00362. DnaA. 1 hit.
PROSITEiPS01008. DNAA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structure and function of the region of the replication origin of the Bacillus subtilis chromosome. III. Nucleotide sequence of some 10,000 base pairs in the origin region."
    Moriya S., Ogasawara N., Yoshikawa H.
    Nucleic Acids Res. 13:2251-2265(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Systematic sequencing of the 180 kilobase region of the Bacillus subtilis chromosome containing the replication origin."
    Ogasawara N., Nakai S., Yoshikawa H.
    DNA Res. 1:1-14(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168.
  3. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.
  4. "Regulation of initiation of the chromosomal replication by DnaA-boxes in the origin region of the Bacillus subtilis chromosome."
    Moriya S., Fukuoka T., Ogasawara N., Yoshikawa H.
    EMBO J. 7:2911-2917(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-6 AND 441-446.
    Strain: 168 / PSL1.

Entry informationi

Entry nameiDNAA_BACSU
AccessioniPrimary (citable) accession number: P05648
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: November 1, 1988
Last modified: February 17, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.