Reviewed, UniProtKB/Swiss-Prot P05637 (APAH_ECOLI)

Names and origin

Protein names

Recommended name:
    Bis(5'-nucleosyl)-tetraphosphatase [symmetrical]
    EC=3.6.1.41
Alternative name(s):
    Diadenosine tetraphosphatase
    Ap4A hydrolase
    Diadenosine 5',5'''-P1,P4-tetraphosphate pyrophosphohydrolase

Gene names

Name:	apaH
Ordered Locus Names:	b0049, JW0048

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	280 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Hydrolyzes diadenosine 5',5'''-P1,P4-tetraphosphate to yield ADP. HAMAP MF_00199
Catalytic activity	P1,P(4)-bis(5'-adenosyl) tetraphosphate + H2O = 2 ADP. HAMAP MF_00199
Enzyme regulation	Co2+ is a strong stimulator (100-fold increase in rate of hydrolysis). Mn2+, Cd2+, Ni2+, Mg2+ and Ca2+ are weak stimulators; the two latter act synergistically with Co2+. HAMAP MF_00199
Subunit structure	Monomer. HAMAP MF_00199
Sequence similarities	Belongs to the Ap4A hydrolase family.

Ontologies

Keywords
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 280	280	Bis(5'-nucleosyl)-tetraphosphatase [symmetrical] HAMAP MF_00199		PRO_0000197988
Experimental info
	Sequence conflict	83	1	K → L Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P05637-1 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: BD6560C73446C1BB	FASTA	280	31,297
	10 20 30 40 50 60 MATYLIGDVH GCYDELIALL HKVEFTPGKD TLWLTGDLVA RGPGSLDVLR YVKSLGDSVR 70 80 90 100 110 120 LVLGNHDLHL LAVFAGISRN KPKDRLTPLL EAPDADELLN WLRRQPLLQI DEEKKLVMAH 130 140 150 160 170 180 AGITPQWDLQ TAKECARDVE AVLSSDSYPF FLDAMYGDMP NNWSPELRGL GRLRFITNAF 190 200 210 220 230 240 TRMRFCFPNG QLDMYSKESP EEAPAPLKPW FAIPGPVAEE YSIAFGHWAS LEGKGTPEGI 250 260 270 280 YALDTGCCWG GTLTCLRWED KQYFVQPSNR HKDLGEAAAS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The gene for Escherichia coli diadenosine tetraphosphatase is located immediately clockwise to folA and forms an operon with ksgA." Blanchin-Roland S., Blanquet S., Schmitter J.-M., Fayat G. Mol. Gen. Genet. 205:515-522(1986) [PubMed: 3031429] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region." Yura T., Mori H., Nagai H., Nagata T., Ishihama A., Fujita N., Isono K., Mizobuchi K., Nakata A. Nucleic Acids Res. 20:3305-3308(1992) [PubMed: 1630901] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION TO 83 AND 275. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Catabolism of diadenosine 5',5''-P1,P4-tetraphosphate in procaryotes. Purification and properties of diadenosine 5',5''-P1,P4-tetraphosphate (symmetrical) pyrophosphohydrolase from Escherichia coli K12." Guranowski A., Jakubowski H., Holler E. J. Biol. Chem. 258:14784-14789(1983) [PubMed: 6317672] [Abstract] Cited for: CHARACTERIZATION. Strain: K12.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X04711 Genomic DNA. Translation: CAA28416.1. U00096 Genomic DNA. Translation: AAC73160.1. AP009048 Genomic DNA. Translation: BAB96617.2.
PIR	A64726.
RefSeq	AP_000713.1. NP_414591.1.
3D structure databases
SMR	P05637. Positions 2-268.
ModBase	Search...
2-D gel databases
ECO2DBASE	C031.3. 6TH EDITION.
Genome annotation databases
GeneID	944770.
GenomeReviews	Gene locus JW0048 in contig AP009048_GR. Gene locus b0049 in contig U00096_GR.
KEGG	ecj:JW0048. eco:b0049.
Organism-specific databases
EchoBASE	EB0046.
EcoGene	EG10048. apaH.
CMR	Search...
Phylogenomic databases
HOGENOM	P05637.
OMA	P05637. LVFGHWA.
Enzyme and pathway databases
BioCyc	EcoCyc:EG10048-MON. MetaCyc:EG10048-MON.
Family and domain databases
HAMAP	MF_00199. [Tree]
InterPro	IPR004617. ApaH. IPR004843. M-pesterase. [Graphical view]
Pfam	PF00149. Metallophos. 1 hit. [Graphical view]
PIRSF	PIRSF000903. B5n-ttraPtase_sm. 1 hit.
TIGRFAMs	TIGR00668. apaH. 1 hit.
ProtoNet	Search...

Entry information

Entry name

APAH_ECOLI

Accession

Primary (citable) accession number: P05637
Secondary accession number(s): P78039

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1988
Last sequence update:	November 1, 1997
Last modified:	June 16, 2009
This is version 83 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)