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Protein

Bis(5'-nucleosyl)-tetraphosphatase [symmetrical]

Gene

apaH

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes diadenosine 5',5'''-P1,P4-tetraphosphate to yield ADP.1 Publication

Catalytic activityi

P1,P4-bis(5'-adenosyl) tetraphosphate + H2O = 2 ADP.1 Publication

Enzyme regulationi

Co2+ is a strong stimulator (100-fold increase in rate of hydrolysis). Mn2+, Cd2+, Ni2+, Mg2+ and Ca2+ are weak stimulators; the two latter act synergistically with Co2+.1 Publication

GO - Molecular functioni

  • bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity Source: EcoliWiki
  • bis(5'-nucleosyl)-tetraphosphatase activity Source: EcoliWiki
  • protein tyrosine/serine/threonine phosphatase activity Source: GO_Central

GO - Biological processi

  • nucleobase-containing small molecule interconversion Source: EcoliWiki
  • protein dephosphorylation Source: GO_Central
  • response to X-ray Source: EcoCyc

Keywordsi

Molecular functionHydrolase

Enzyme and pathway databases

BioCyciEcoCyc:EG10048-MONOMER.
MetaCyc:EG10048-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Bis(5'-nucleosyl)-tetraphosphatase [symmetrical] (EC:3.6.1.41)
Alternative name(s):
Ap4A hydrolase
Diadenosine 5',5'''-P1,P4-tetraphosphate pyrophosphohydrolase
Diadenosine tetraphosphatase
Gene namesi
Name:apaH
Ordered Locus Names:b0049, JW0048
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10048. apaH.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001979881 – 280Bis(5'-nucleosyl)-tetraphosphatase [symmetrical]Add BLAST280

Proteomic databases

PaxDbiP05637.
PRIDEiP05637.

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

BioGridi4262205. 12 interactors.
DIPiDIP-9113N.
IntActiP05637. 5 interactors.
STRINGi316385.ECDH10B_0050.

Structurei

3D structure databases

ProteinModelPortaliP05637.
SMRiP05637.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Ap4A hydrolase family.Curated

Phylogenomic databases

eggNOGiENOG4105DDT. Bacteria.
COG0639. LUCA.
HOGENOMiHOG000251871.
InParanoidiP05637.
KOiK01525.
PhylomeDBiP05637.

Family and domain databases

CDDicd07422. MPP_ApaH. 1 hit.
Gene3Di3.60.21.10. 1 hit.
HAMAPiMF_00199. ApaH. 1 hit.
InterProiView protein in InterPro
IPR004617. ApaH.
IPR004843. Calcineurin-like_PHP_ApaH.
IPR029052. Metallo-depent_PP-like.
PANTHERiPTHR24032:SF49. PTHR24032:SF49. 1 hit.
PfamiView protein in Pfam
PF00149. Metallophos. 1 hit.
PIRSFiPIRSF000903. B5n-ttraPtase_sm. 1 hit.
SUPFAMiSSF56300. SSF56300. 1 hit.
TIGRFAMsiTIGR00668. apaH. 1 hit.

Sequencei

Sequence statusi: Complete.

P05637-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATYLIGDVH GCYDELIALL HKVEFTPGKD TLWLTGDLVA RGPGSLDVLR
60 70 80 90 100
YVKSLGDSVR LVLGNHDLHL LAVFAGISRN KPKDRLTPLL EAPDADELLN
110 120 130 140 150
WLRRQPLLQI DEEKKLVMAH AGITPQWDLQ TAKECARDVE AVLSSDSYPF
160 170 180 190 200
FLDAMYGDMP NNWSPELRGL GRLRFITNAF TRMRFCFPNG QLDMYSKESP
210 220 230 240 250
EEAPAPLKPW FAIPGPVAEE YSIAFGHWAS LEGKGTPEGI YALDTGCCWG
260 270 280
GTLTCLRWED KQYFVQPSNR HKDLGEAAAS
Length:280
Mass (Da):31,297
Last modified:November 1, 1997 - v2
Checksum:iBD6560C73446C1BB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti83K → L in CAA28416 (PubMed:3031429).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04711 Genomic DNA. Translation: CAA28416.1.
U00096 Genomic DNA. Translation: AAC73160.1.
AP009048 Genomic DNA. Translation: BAB96617.2.
PIRiA64726.
RefSeqiNP_414591.1. NC_000913.3.
WP_000257192.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73160; AAC73160; b0049.
BAB96617; BAB96617; BAB96617.
GeneIDi944770.
KEGGiecj:JW0048.
eco:b0049.
PATRICifig|1411691.4.peg.2234.

Similar proteinsi

Entry informationi

Entry nameiAPAH_ECOLI
AccessioniPrimary (citable) accession number: P05637
Secondary accession number(s): P78039
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: November 1, 1997
Last modified: August 30, 2017
This is version 145 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families