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Protein

Bis(5'-nucleosyl)-tetraphosphatase [symmetrical]

Gene

apaH

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes diadenosine 5',5'''-P1,P4-tetraphosphate to yield ADP.1 Publication

Catalytic activityi

P1,P(4)-bis(5'-adenosyl) tetraphosphate + H2O = 2 ADP.1 Publication

Enzyme regulationi

Co2+ is a strong stimulator (100-fold increase in rate of hydrolysis). Mn2+, Cd2+, Ni2+, Mg2+ and Ca2+ are weak stimulators; the two latter act synergistically with Co2+.1 Publication

GO - Molecular functioni

  • bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity Source: EcoliWiki
  • bis(5'-nucleosyl)-tetraphosphatase activity Source: EcoliWiki

GO - Biological processi

  • nucleobase-containing small molecule interconversion Source: EcoliWiki
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

BioCyciEcoCyc:EG10048-MONOMER.
ECOL316407:JW0048-MONOMER.
MetaCyc:EG10048-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Bis(5'-nucleosyl)-tetraphosphatase [symmetrical] (EC:3.6.1.41)
Alternative name(s):
Ap4A hydrolase
Diadenosine 5',5'''-P1,P4-tetraphosphate pyrophosphohydrolase
Diadenosine tetraphosphatase
Gene namesi
Name:apaH
Ordered Locus Names:b0049, JW0048
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10048. apaH.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001979881 – 280Bis(5'-nucleosyl)-tetraphosphatase [symmetrical]Add BLAST280

Proteomic databases

PaxDbiP05637.
PRIDEiP05637.

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

BioGridi4262205. 12 interactors.
DIPiDIP-9113N.
IntActiP05637. 5 interactors.
STRINGi511145.b0049.

Structurei

3D structure databases

ProteinModelPortaliP05637.
SMRiP05637.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Ap4A hydrolase family.Curated

Phylogenomic databases

eggNOGiENOG4105DDT. Bacteria.
COG0639. LUCA.
HOGENOMiHOG000251871.
InParanoidiP05637.
KOiK01525.
OMAiINAFTRM.
PhylomeDBiP05637.

Family and domain databases

CDDicd07422. MPP_ApaH. 1 hit.
Gene3Di3.60.21.10. 2 hits.
HAMAPiMF_00199. ApaH. 1 hit.
InterProiIPR004617. ApaH.
IPR004843. Calcineurin-like_PHP_ApaH.
IPR029052. Metallo-depent_PP-like.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PIRSFiPIRSF000903. B5n-ttraPtase_sm. 1 hit.
SUPFAMiSSF56300. SSF56300. 1 hit.
TIGRFAMsiTIGR00668. apaH. 1 hit.

Sequencei

Sequence statusi: Complete.

P05637-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATYLIGDVH GCYDELIALL HKVEFTPGKD TLWLTGDLVA RGPGSLDVLR
60 70 80 90 100
YVKSLGDSVR LVLGNHDLHL LAVFAGISRN KPKDRLTPLL EAPDADELLN
110 120 130 140 150
WLRRQPLLQI DEEKKLVMAH AGITPQWDLQ TAKECARDVE AVLSSDSYPF
160 170 180 190 200
FLDAMYGDMP NNWSPELRGL GRLRFITNAF TRMRFCFPNG QLDMYSKESP
210 220 230 240 250
EEAPAPLKPW FAIPGPVAEE YSIAFGHWAS LEGKGTPEGI YALDTGCCWG
260 270 280
GTLTCLRWED KQYFVQPSNR HKDLGEAAAS
Length:280
Mass (Da):31,297
Last modified:November 1, 1997 - v2
Checksum:iBD6560C73446C1BB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti83K → L in CAA28416 (PubMed:3031429).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04711 Genomic DNA. Translation: CAA28416.1.
U00096 Genomic DNA. Translation: AAC73160.1.
AP009048 Genomic DNA. Translation: BAB96617.2.
PIRiA64726.
RefSeqiNP_414591.1. NC_000913.3.
WP_000257192.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73160; AAC73160; b0049.
BAB96617; BAB96617; BAB96617.
GeneIDi944770.
KEGGiecj:JW0048.
eco:b0049.
PATRICi32115197. VBIEscCol129921_0050.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04711 Genomic DNA. Translation: CAA28416.1.
U00096 Genomic DNA. Translation: AAC73160.1.
AP009048 Genomic DNA. Translation: BAB96617.2.
PIRiA64726.
RefSeqiNP_414591.1. NC_000913.3.
WP_000257192.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP05637.
SMRiP05637.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262205. 12 interactors.
DIPiDIP-9113N.
IntActiP05637. 5 interactors.
STRINGi511145.b0049.

Proteomic databases

PaxDbiP05637.
PRIDEiP05637.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73160; AAC73160; b0049.
BAB96617; BAB96617; BAB96617.
GeneIDi944770.
KEGGiecj:JW0048.
eco:b0049.
PATRICi32115197. VBIEscCol129921_0050.

Organism-specific databases

EchoBASEiEB0046.
EcoGeneiEG10048. apaH.

Phylogenomic databases

eggNOGiENOG4105DDT. Bacteria.
COG0639. LUCA.
HOGENOMiHOG000251871.
InParanoidiP05637.
KOiK01525.
OMAiINAFTRM.
PhylomeDBiP05637.

Enzyme and pathway databases

BioCyciEcoCyc:EG10048-MONOMER.
ECOL316407:JW0048-MONOMER.
MetaCyc:EG10048-MONOMER.

Miscellaneous databases

PROiP05637.

Family and domain databases

CDDicd07422. MPP_ApaH. 1 hit.
Gene3Di3.60.21.10. 2 hits.
HAMAPiMF_00199. ApaH. 1 hit.
InterProiIPR004617. ApaH.
IPR004843. Calcineurin-like_PHP_ApaH.
IPR029052. Metallo-depent_PP-like.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PIRSFiPIRSF000903. B5n-ttraPtase_sm. 1 hit.
SUPFAMiSSF56300. SSF56300. 1 hit.
TIGRFAMsiTIGR00668. apaH. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiAPAH_ECOLI
AccessioniPrimary (citable) accession number: P05637
Secondary accession number(s): P78039
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: November 1, 1997
Last modified: November 30, 2016
This is version 139 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.