P05637 (APAH_ECOLI) Reviewed, UniProtKB/Swiss-Prot
Last modified
January 25, 2012.
Version 106.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Bis(5'-nucleosyl)-tetraphosphatase [symmetrical] EC=3.6.1.41 Alternative name(s): Ap4A hydrolase Diadenosine 5',5'''-P1,P4-tetraphosphate pyrophosphohydrolase Diadenosine tetraphosphatase | ||||
| Gene names |
| ||||
| Organism | Escherichia coli (strain K12) | ||||
| Taxonomic identifier | 83333 [NCBI] | ||||
| Taxonomic lineage | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia |
Protein attributes
| Sequence length | 280 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Hydrolyzes diadenosine 5',5'''-P1,P4-tetraphosphate to yield ADP. HAMAP MF_00199 |
| Catalytic activity | P1,P(4)-bis(5'-adenosyl) tetraphosphate + H2O = 2 ADP. HAMAP MF_00199 |
| Enzyme regulation | Co2+ is a strong stimulator (100-fold increase in rate of hydrolysis). Mn2+, Cd2+, Ni2+, Mg2+ and Ca2+ are weak stimulators; the two latter act synergistically with Co2+. HAMAP MF_00199 |
| Subunit structure | Monomer. |
| Sequence similarities | Belongs to the Ap4A hydrolase family. |
Ontologies
| Keywords | |
|---|---|
| Molecular function | Hydrolase |
| Technical term | Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Molecular function | bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity Inferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 280 | 280 | Bis(5'-nucleosyl)-tetraphosphatase [symmetrical] HAMAP MF_00199 | PRO_0000197988 | |||||
Experimental info | |||||||||
| Sequence conflict | 83 | 1 | K → L in CAA28416. Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "The gene for Escherichia coli diadenosine tetraphosphatase is located immediately clockwise to folA and forms an operon with ksgA." Blanchin-Roland S., Blanquet S., Schmitter J.-M., Fayat G. Mol. Gen. Genet. 205:515-522(1986) [PubMed: 3031429] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [2] | "Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region." Yura T., Mori H., Nagai H., Nagata T., Ishihama A., Fujita N., Isono K., Mizobuchi K., Nakata A. Nucleic Acids Res. 20:3305-3308(1992) [PubMed: 1630901] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12. |
| [3] | "The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076. |
| [4] | "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION TO 83 AND 275. Strain: K12 / W3110 / ATCC 27325 / DSM 5911. |
| [5] | "Catabolism of diadenosine 5',5''-P1,P4-tetraphosphate in procaryotes. Purification and properties of diadenosine 5',5''-P1,P4-tetraphosphate (symmetrical) pyrophosphohydrolase from Escherichia coli K12." Guranowski A., Jakubowski H., Holler E. J. Biol. Chem. 258:14784-14789(1983) [PubMed: 6317672] [Abstract] Cited for: CHARACTERIZATION. Strain: K12. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | X04711 Genomic DNA. Translation: CAA28416.1. U00096 Genomic DNA. Translation: AAC73160.1. AP009048 Genomic DNA. Translation: BAB96617.2. |
| PIR | A64726. |
| RefSeq | NP_414591.1. NC_000913.2. |
3D structure databases | |
| ProteinModelPortal | P05637. |
| SMR | P05637. Positions 2-268. |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP-9113N. |
| IntAct | P05637. 2 interactions. |
2D gel databases | |
| ECO2DBASE | C031.3. 6TH EDITION. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblBacteria | EBESCT00000004303; EBESCP00000004303; EBESCG00000003508. EBESCT00000004304; EBESCP00000004304; EBESCG00000003508. EBESCT00000004305; EBESCP00000004305; EBESCG00000003508. EBESCT00000004306; EBESCP00000004306; EBESCG00000003508. EBESCT00000017189; EBESCP00000016480; EBESCG00000016248. |
| GeneID | 944770. |
| GenomeReviews | Gene locus JW0048 in contig AP009048_GR. Gene locus b0049 in contig U00096_GR. |
| KEGG | ecj:JW0048. eco:b0049. |
| PATRIC | 32115197. VBIEscCol129921_0050. |
Organism-specific databases | |
| EchoBASE | EB0046. |
| EcoGene | EG10048. apaH. |
Phylogenomic databases | |
| eggNOG | COG0639. |
| GeneTree | EBGT00050000010576. |
| HOGENOM | HBG724457. |
| OMA | LVFGHWA. |
| PhylomeDB | P05637. |
| ProtClustDB | PRK00166. |
Enzyme and pathway databases | |
| BioCyc | EcoCyc:EG10048-MONOMER. MetaCyc:EG10048-MONOMER. |
Gene expression databases | |
| Genevestigator | P05637. |
Family and domain databases | |
| HAMAP | MF_00199. ApaH. [Tree] |
| InterPro | IPR004617. ApaH. IPR004843. Metallo_PEstase_dom. [Graphical view] |
| KO | K01525. |
| Pfam | PF00149. Metallophos. 1 hit. [Graphical view] |
| PIRSF | PIRSF000903. B5n-ttraPtase_sm. 1 hit. |
| TIGRFAMs | TIGR00668. ApaH. 1 hit. |
| ProtoNet | Search... |
Entry information
| Entry name | APAH_ECOLI | ||||||||
| Accession | Primary (citable) accession number: P05637 Secondary accession number(s): P78039 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Prokaryotic Protein Annotation Program | ||||||||
Relevant documents
| Escherichia coli Escherichia coli (strain K12): entries and cross-references to EcoGene |
| SIMILARITY comments Index of protein domains and families |

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