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P05627

- JUN_MOUSE

UniProt

P05627 - JUN_MOUSE

Protein

Transcription factor AP-1

Gene

Jun

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 149 (01 Oct 2014)
      Sequence version 3 (01 Oct 1989)
      Previous versions | rss
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    Functioni

    Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation.2 Publications

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei275 – 2751Necessary for syngernistic transcriptional activity with SMAD3By similarity

    GO - Molecular functioni

    1. cAMP response element binding Source: Ensembl
    2. DNA binding Source: MGI
    3. double-stranded DNA binding Source: Ensembl
    4. protein binding Source: IntAct
    5. Rho GTPase activator activity Source: Ensembl
    6. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: Ensembl
    7. RNA polymerase II distal enhancer sequence-specific DNA binding Source: Ensembl
    8. transcription coactivator activity Source: Ensembl
    9. transcription regulatory region DNA binding Source: MGI

    GO - Biological processi

    1. aging Source: Ensembl
    2. angiogenesis Source: MGI
    3. axon regeneration Source: MGI
    4. cellular process Source: MGI
    5. cellular response to calcium ion Source: MGI
    6. cellular response to potassium ion starvation Source: Ensembl
    7. circadian rhythm Source: Ensembl
    8. leading edge cell differentiation Source: MGI
    9. learning Source: Ensembl
    10. liver development Source: MGI
    11. membrane depolarization Source: Ensembl
    12. microglial cell activation Source: MGI
    13. monocyte differentiation Source: MGI
    14. negative regulation by host of viral transcription Source: Ensembl
    15. negative regulation of apoptotic process Source: MGI
    16. negative regulation of cell proliferation Source: MGI
    17. negative regulation of DNA binding Source: Ensembl
    18. negative regulation of neuron apoptotic process Source: MGI
    19. negative regulation of protein autophosphorylation Source: MGI
    20. negative regulation of transcription, DNA-templated Source: Ensembl
    21. outflow tract morphogenesis Source: MGI
    22. positive regulation by host of viral transcription Source: Ensembl
    23. positive regulation of DNA replication Source: Ensembl
    24. positive regulation of endothelial cell proliferation Source: MGI
    25. positive regulation of fibroblast proliferation Source: MGI
    26. positive regulation of monocyte differentiation Source: Ensembl
    27. positive regulation of neuron apoptotic process Source: Ensembl
    28. positive regulation of smooth muscle cell proliferation Source: Ensembl
    29. positive regulation of transcription from RNA polymerase II promoter Source: MGI
    30. regulation of cell cycle Source: MGI
    31. regulation of transcription, DNA-templated Source: MGI
    32. release of cytochrome c from mitochondria Source: Ensembl
    33. response to cAMP Source: Ensembl
    34. response to cytokine Source: Ensembl
    35. response to drug Source: MGI
    36. response to hydrogen peroxide Source: Ensembl
    37. response to lipopolysaccharide Source: Ensembl
    38. response to mechanical stimulus Source: Ensembl
    39. response to radiation Source: Ensembl
    40. SMAD protein import into nucleus Source: Ensembl
    41. SMAD protein signal transduction Source: Ensembl
    42. transforming growth factor beta receptor signaling pathway Source: Ensembl

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    ReactomeiREACT_188530. FCERI mediated MAPK activation.
    REACT_188970. Oxidative Stress Induced Senescence.
    REACT_204811. Activation of the AP-1 family of transcription factors.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transcription factor AP-1
    Alternative name(s):
    AH119
    Activator protein 1
    Short name:
    AP1
    Proto-oncogene c-Jun
    V-jun avian sarcoma virus 17 oncogene homolog
    Short name:
    Jun A
    Gene namesi
    Name:Jun
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 4

    Organism-specific databases

    MGIiMGI:96646. Jun.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: Ensembl
    2. nuclear euchromatin Source: Ensembl
    3. nucleus Source: MGI
    4. transcriptional repressor complex Source: MGI
    5. transcription factor complex Source: MGI

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Keywords - Diseasei

    Proto-oncogene

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 334334Transcription factor AP-1PRO_0000076430Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21Phosphothreonine; by PAK2By similarity
    Modified residuei8 – 81Phosphothreonine; by PAK2By similarity
    Modified residuei56 – 561N6-acetyllysine1 Publication
    Modified residuei58 – 581PhosphoserineBy similarity
    Modified residuei63 – 631Phosphoserine; alternate2 Publications
    Modified residuei63 – 631Phosphoserine; by MAPK8 and PLK3; alternateBy similarity
    Modified residuei73 – 731Phosphoserine; by MAPK8 and PLK31 Publication
    Modified residuei89 – 891Phosphothreonine; by PAK2By similarity
    Modified residuei93 – 931Phosphothreonine; by PAK2By similarity
    Modified residuei242 – 2421Phosphothreonine; by GSK3-betaBy similarity
    Modified residuei246 – 2461Phosphoserine; by DYRK2 and GSK3-betaBy similarity
    Modified residuei252 – 2521Phosphoserine; by GSK3-betaBy similarity
    Modified residuei274 – 2741N6-acetyllysineBy similarity
    Modified residuei289 – 2891Phosphothreonine; by PAK2By similarity

    Post-translational modificationi

    Phosphorylated by CaMK4 and PRKDC; phosphorylation enhances the transcriptional activity. Phosphorylated by HIPK3. Phosphorylated by DYRK2 at Ser-246; this primes the protein for subsequent phosphorylation by GSK3B at Thr-242. Phosphorylated at Thr-242, Ser-246 and Ser-252 by GSK3B; phosphorylation reduces its ability to bind DNA. Phosphorylated by PAK2 at Thr-2, Thr-8, Thr-89, Thr-93 and Thr-289 thereby promoting JUN-mediated cell proliferation and transformation. Phosphorylated by PLK3 following hypoxia or UV irradiation, leading to increase DNA-binding activity By similarity.By similarity
    Acetylated at Lys-271 by EP300.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PaxDbiP05627.
    PRIDEiP05627.

    PTM databases

    PhosphoSiteiP05627.

    Expressioni

    Gene expression databases

    ArrayExpressiP05627.
    BgeeiP05627.
    CleanExiMM_JUN.
    GenevestigatoriP05627.

    Interactioni

    Subunit structurei

    Heterodimer with either FOS or BATF3 or ATF7 By similarity. The ATF7/JUN heterodimer is essential for ATF7 transactivation activity. Interacts with SP1, SPIB and TCF20. Interacts with COPS5; the interaction leads indirectly to its phosphorylation. Component of the SMAD3/SMAD4/JUN/FOS/complex which forms at the AP1 promoter site. The SMAD3/SMAD4 heterodimer acts syngernistically with the JUN/FOS heterodimer to activate transcription in response to TGF-beta By similarity. Interacts (via its basic DNA binding and leucine zipper domains) with SMAD3 (via an N-terminal domain); the interaction is required for TGF-beta-mediated transactivation of the SMAD3/SMAD4/JUN/FOS/complex By similarity. Interacts with DSIPI; the interaction inhibits the binding of active AP1 to its target DNA. Interacts with HIVEP3 and MYBBP1A. Interacts with methylated RNF187. Binds to HIPK3.By similarity4 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Cdk6Q642612EBI-764369,EBI-847380
    ESR1P033726EBI-764369,EBI-78473From a different organism.
    Mapk8Q91Y862EBI-764369,EBI-298784

    Protein-protein interaction databases

    BioGridi200871. 40 interactions.
    DIPiDIP-1068N.
    IntActiP05627. 5 interactions.
    MINTiMINT-209953.

    Structurei

    3D structure databases

    ProteinModelPortaliP05627.
    SMRiP05627. Positions 260-311.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini255 – 31864bZIPPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni255 – 28228Basic motifPROSITE-ProRule annotationAdd
    BLAST
    Regioni283 – 31129Leucine-zipperPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the bZIP family. Jun subfamily.Curated
    Contains 1 bZIP (basic-leucine zipper) domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG283376.
    HOGENOMiHOG000006648.
    HOVERGENiHBG001722.
    InParanoidiP05627.
    KOiK04448.
    OMAiAELHNQN.
    OrthoDBiEOG75MVXV.
    PhylomeDBiP05627.
    TreeFamiTF323952.

    Family and domain databases

    Gene3Di1.10.880.10. 1 hit.
    InterProiIPR004827. bZIP.
    IPR005643. JNK.
    IPR002112. Leuzip_Jun.
    IPR008917. TF_DNA-bd.
    [Graphical view]
    PfamiPF00170. bZIP_1. 1 hit.
    PF03957. Jun. 1 hit.
    [Graphical view]
    PRINTSiPR00043. LEUZIPPRJUN.
    SMARTiSM00338. BRLZ. 1 hit.
    [Graphical view]
    SUPFAMiSSF47454. SSF47454. 1 hit.
    PROSITEiPS50217. BZIP. 1 hit.
    PS00036. BZIP_BASIC. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P05627-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTAKMETTFY DDALNASFLQ SESGAYGYSN PKILKQSMTL NLADPVGSLK    50
    PHLRAKNSDL LTSPDVGLLK LASPELERLI IQSSNGHITT TPTPTQFLCP 100
    KNVTDEQEGF AEGFVRALAE LHSQNTLPSV TSAAQPVSGA GMVAPAVASV 150
    AGAGGGGGYS ASLHSEPPVY ANLSNFNPGA LSSGGGAPSY GAAGLAFPSQ 200
    PQQQQQPPQP PHHLPQQIPV QHPRLQALKE EPQTVPEMPG ETPPLSPIDM 250
    ESQERIKAER KRMRNRIAAS KCRKRKLERI ARLEEKVKTL KAQNSELAST 300
    ANMLREQVAQ LKQKVMNHVN SGCQLMLTQQ LQTF 334
    Length:334
    Mass (Da):35,944
    Last modified:October 1, 1989 - v3
    Checksum:i2BE0E4025B8B1CA3
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti183 – 1831S → C in CAA31252. (PubMed:3136397)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X12761 mRNA. Translation: CAA31252.1.
    X12740 mRNA. Translation: CAA31236.1.
    J04115 mRNA. Translation: AAA37419.1.
    BC002081 mRNA. Translation: AAH02081.1.
    BC021888 mRNA. Translation: AAH21888.1.
    CCDSiCCDS18364.1.
    PIRiA31345. TVMSJA.
    RefSeqiNP_034721.1. NM_010591.2.
    UniGeneiMm.275071.

    Genome annotation databases

    EnsembliENSMUST00000058555; ENSMUSP00000054270; ENSMUSG00000052684.
    ENSMUST00000107094; ENSMUSP00000102711; ENSMUSG00000052684.
    GeneIDi16476.
    KEGGimmu:16476.
    UCSCiuc008tsq.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X12761 mRNA. Translation: CAA31252.1 .
    X12740 mRNA. Translation: CAA31236.1 .
    J04115 mRNA. Translation: AAA37419.1 .
    BC002081 mRNA. Translation: AAH02081.1 .
    BC021888 mRNA. Translation: AAH21888.1 .
    CCDSi CCDS18364.1.
    PIRi A31345. TVMSJA.
    RefSeqi NP_034721.1. NM_010591.2.
    UniGenei Mm.275071.

    3D structure databases

    ProteinModelPortali P05627.
    SMRi P05627. Positions 260-311.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 200871. 40 interactions.
    DIPi DIP-1068N.
    IntActi P05627. 5 interactions.
    MINTi MINT-209953.

    Chemistry

    ChEMBLi CHEMBL5369.

    PTM databases

    PhosphoSitei P05627.

    Proteomic databases

    PaxDbi P05627.
    PRIDEi P05627.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000058555 ; ENSMUSP00000054270 ; ENSMUSG00000052684 .
    ENSMUST00000107094 ; ENSMUSP00000102711 ; ENSMUSG00000052684 .
    GeneIDi 16476.
    KEGGi mmu:16476.
    UCSCi uc008tsq.2. mouse.

    Organism-specific databases

    CTDi 3725.
    MGIi MGI:96646. Jun.

    Phylogenomic databases

    eggNOGi NOG283376.
    HOGENOMi HOG000006648.
    HOVERGENi HBG001722.
    InParanoidi P05627.
    KOi K04448.
    OMAi AELHNQN.
    OrthoDBi EOG75MVXV.
    PhylomeDBi P05627.
    TreeFami TF323952.

    Enzyme and pathway databases

    Reactomei REACT_188530. FCERI mediated MAPK activation.
    REACT_188970. Oxidative Stress Induced Senescence.
    REACT_204811. Activation of the AP-1 family of transcription factors.

    Miscellaneous databases

    NextBioi 289763.
    PROi P05627.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P05627.
    Bgeei P05627.
    CleanExi MM_JUN.
    Genevestigatori P05627.

    Family and domain databases

    Gene3Di 1.10.880.10. 1 hit.
    InterProi IPR004827. bZIP.
    IPR005643. JNK.
    IPR002112. Leuzip_Jun.
    IPR008917. TF_DNA-bd.
    [Graphical view ]
    Pfami PF00170. bZIP_1. 1 hit.
    PF03957. Jun. 1 hit.
    [Graphical view ]
    PRINTSi PR00043. LEUZIPPRJUN.
    SMARTi SM00338. BRLZ. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47454. SSF47454. 1 hit.
    PROSITEi PS50217. BZIP. 1 hit.
    PS00036. BZIP_BASIC. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Transcriptional activation of c-jun during the G0/G1 transition in mouse fibroblasts."
      Ryseck R.-P., Hirai S., Yaniv M., Bravo R.
      Nature 334:535-537(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Induction of proto-oncogene JUN/AP-1 by serum and TPA."
      Lamph W.W., Wamsley P., Sassone-Corsi P., Verma I.M.
      Nature 334:629-631(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "Induction of protooncogene c-jun by serum growth factors."
      Ryder K., Nathans D.
      Proc. Natl. Acad. Sci. U.S.A. 85:8464-8467(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Mammary tumor.
    5. "Molecular cloning reveals that the p160 Myb-binding protein is a novel, predominantly nucleolar protein which may play a role in transactivation by Myb."
      Tavner F.J., Simpson R., Tashiro S., Favier D., Jenkins N.A., Gilbert D.J., Copeland N.G., Macmillan E.M., Lutwyche J., Keough R.A., Ishii S., Gonda T.J.
      Mol. Cell. Biol. 18:989-1002(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH MYBBP1A.
    6. "Inhibition of AP-1 by the glucocorticoid-inducible protein GILZ."
      Mittelstadt P.R., Ashwell J.D.
      J. Biol. Chem. 276:29603-29610(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH DSIPI.
    7. "Schnurri-3 (KRC) interacts with c-Jun to regulate the IL-2 gene in T cells."
      Oukka M., Wein M.N., Glimcher L.H.
      J. Exp. Med. 199:15-24(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH HIVEP3, FUNCTION.
    8. "Cyclic AMP stimulates SF-1-dependent CYP11A1 expression through homeodomain-interacting protein kinase 3-mediated Jun N-terminal kinase and c-Jun phosphorylation."
      Lan H.-C., Li H.-J., Lin G., Lai P.-Y., Chung B.-C.
      Mol. Cell. Biol. 27:2027-2036(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, PHOSPHORYLATION BY HIPK3.
    9. "The phagosomal proteome in interferon-gamma-activated macrophages."
      Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
      Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-63, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. "Identification of a co-activator that links growth factor signalling to c-Jun/AP-1 activation."
      Davies C.C., Chakraborty A., Cipriani F., Haigh K., Haigh J.J., Behrens A.
      Nat. Cell Biol. 12:963-972(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH RNF187.
    11. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
      Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
      Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-56, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic fibroblast.

    Entry informationi

    Entry nameiJUN_MOUSE
    AccessioniPrimary (citable) accession number: P05627
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1988
    Last sequence update: October 1, 1989
    Last modified: October 1, 2014
    This is version 149 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3