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Protein

Transcription factor AP-1

Gene

Jun

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells. Binds to the USP28 promoter in colorectal cancer (CRC) cells (By similarity).By similarity2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei275Necessary for synergistic transcriptional activity with SMAD3By similarity1

GO - Molecular functioni

GO - Biological processi

  • aging Source: Ensembl
  • angiogenesis Source: MGI
  • axon regeneration Source: MGI
  • cellular process Source: MGI
  • cellular response to calcium ion Source: MGI
  • cellular response to hormone stimulus Source: GO_Central
  • cellular response to potassium ion starvation Source: Ensembl
  • circadian rhythm Source: Ensembl
  • eyelid development in camera-type eye Source: MGI
  • leading edge cell differentiation Source: MGI
  • learning Source: Ensembl
  • liver development Source: MGI
  • membrane depolarization Source: Ensembl
  • microglial cell activation Source: MGI
  • monocyte differentiation Source: MGI
  • negative regulation by host of viral transcription Source: MGI
  • negative regulation of apoptotic process Source: MGI
  • negative regulation of cell proliferation Source: MGI
  • negative regulation of DNA binding Source: MGI
  • negative regulation of neuron apoptotic process Source: MGI
  • negative regulation of protein autophosphorylation Source: MGI
  • negative regulation of transcription, DNA-templated Source: MGI
  • negative regulation of transcription from RNA polymerase II promoter Source: GO_Central
  • negative regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress Source: MGI
  • outflow tract morphogenesis Source: MGI
  • positive regulation by host of viral transcription Source: MGI
  • positive regulation of cell differentiation Source: GO_Central
  • positive regulation of DNA replication Source: Ensembl
  • positive regulation of DNA-templated transcription, initiation Source: MGI
  • positive regulation of endothelial cell proliferation Source: MGI
  • positive regulation of epithelial cell migration Source: MGI
  • positive regulation of ERK1 and ERK2 cascade Source: MGI
  • positive regulation of fibroblast proliferation Source: MGI
  • positive regulation of monocyte differentiation Source: Ensembl
  • positive regulation of neuron apoptotic process Source: Ensembl
  • positive regulation of pri-miRNA transcription from RNA polymerase II promoter Source: MGI
  • positive regulation of smooth muscle cell proliferation Source: Ensembl
  • positive regulation of transcription, DNA-templated Source: MGI
  • positive regulation of transcription from RNA polymerase II promoter Source: MGI
  • Ras protein signal transduction Source: UniProtKB
  • regulation of cell cycle Source: MGI
  • regulation of transcription, DNA-templated Source: MGI
  • release of cytochrome c from mitochondria Source: Ensembl
  • response to cAMP Source: GO_Central
  • response to cytokine Source: GO_Central
  • response to drug Source: MGI
  • response to hydrogen peroxide Source: Ensembl
  • response to lipopolysaccharide Source: GO_Central
  • response to muscle stretch Source: MGI
  • response to radiation Source: GO_Central
  • SMAD protein import into nucleus Source: MGI
  • SMAD protein signal transduction Source: MGI
  • transforming growth factor beta receptor signaling pathway Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiR-MMU-2559580. Oxidative Stress Induced Senescence.
R-MMU-2871796. FCERI mediated MAPK activation.
R-MMU-450341. Activation of the AP-1 family of transcription factors.
R-MMU-5617472. Activation of anterior HOX genes in hindbrain development during early embryogenesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor AP-1
Alternative name(s):
AH119
Activator protein 1
Short name:
AP1
Proto-oncogene c-Jun
V-jun avian sarcoma virus 17 oncogene homolog
Short name:
Jun A
Gene namesi
Name:Jun
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:96646. Jun.

Subcellular locationi

  • Nucleus By similarity

GO - Cellular componenti

  • cytosol Source: Ensembl
  • nuclear chromatin Source: GO_Central
  • nuclear euchromatin Source: MGI
  • nucleoplasm Source: MGI
  • nucleus Source: MGI
  • transcriptional repressor complex Source: MGI
  • transcription factor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Keywords - Diseasei

Proto-oncogene

Chemistry databases

ChEMBLiCHEMBL5369.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000764301 – 334Transcription factor AP-1Add BLAST334

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2Phosphothreonine; by PAK2By similarity1
Modified residuei8Phosphothreonine; by PAK2By similarity1
Modified residuei56N6-acetyllysineCombined sources1
Modified residuei58PhosphoserineCombined sources1
Modified residuei63PhosphoserineCombined sources1
Modified residuei73Phosphoserine; by MAPK8 and PLK31 Publication1
Modified residuei89Phosphothreonine; by PAK2By similarity1
Modified residuei91PhosphothreonineBy similarity1
Modified residuei93Phosphothreonine; by PAK2By similarity1
Modified residuei242PhosphothreonineCombined sources1
Modified residuei246PhosphoserineCombined sources1
Modified residuei252Phosphoserine; by GSK3-betaBy similarity1
Modified residuei274N6-acetyllysineBy similarity1
Modified residuei289Phosphothreonine; by PAK2By similarity1

Post-translational modificationi

Phosphorylated by CaMK4 and PRKDC; phosphorylation enhances the transcriptional activity. Phosphorylated by HIPK3. Phosphorylated by DYRK2 at Ser-246; this primes the protein for subsequent phosphorylation by GSK3B at Thr-242. Phosphorylated at Thr-242, Ser-246 and Ser-252 by GSK3B; phosphorylation reduces its ability to bind DNA. Phosphorylated by PAK2 at Thr-2, Thr-8, Thr-89, Thr-93 and Thr-289 thereby promoting JUN-mediated cell proliferation and transformation. Phosphorylated by PLK3 following hypoxia or UV irradiation, leading to increase DNA-binding activity (By similarity).By similarity
Ubiquitinated by the SCF(FBXW7), leading to its degradation. Ubiquitination takes place following phosphorylation, that promotes interaction with FBXW7.By similarity
Acetylated at Lys-271 by EP300.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP05627.
PaxDbiP05627.
PeptideAtlasiP05627.
PRIDEiP05627.

PTM databases

iPTMnetiP05627.
PhosphoSitePlusiP05627.

Expressioni

Gene expression databases

BgeeiENSMUSG00000052684.
CleanExiMM_JUN.
ExpressionAtlasiP05627. baseline and differential.
GenevisibleiP05627. MM.

Interactioni

Subunit structurei

Heterodimer with either FOS or BATF3 or ATF7 (By similarity). The ATF7/JUN heterodimer is essential for ATF7 transactivation activity. Interacts with SP1, SPIB and TCF20. Interacts with COPS5; the interaction leads indirectly to its phosphorylation. Component of the SMAD3/SMAD4/JUN/FOS/complex which forms at the AP1 promoter site. The SMAD3/SMAD4 heterodimer acts synergistically with the JUN/FOS heterodimer to activate transcription in response to TGF-beta (By similarity). Interacts (via its basic DNA binding and leucine zipper domains) with SMAD3 (via an N-terminal domain); the interaction is required for TGF-beta-mediated transactivation of the SMAD3/SMAD4/JUN/FOS/complex (By similarity). Interacts with DSIPI; the interaction inhibits the binding of active AP1 to its target DNA (PubMed:11397794). Interacts with HIVEP3 and MYBBP1A (PubMed:9447996, PubMed:14707112). Interacts with methylated RNF187 (PubMed:20852630). Binds to HIPK3. Interacts (when phosphorylated) with FBXW7 (By similarity).By similarity4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Cdk6Q642612EBI-764369,EBI-847380
ESR1P033726EBI-764369,EBI-78473From a different organism.
Mapk8Q91Y862EBI-764369,EBI-298784

GO - Molecular functioni

Protein-protein interaction databases

BioGridi200871. 39 interactors.
DIPiDIP-1068N.
IntActiP05627. 5 interactors.
MINTiMINT-209953.
STRINGi10090.ENSMUSP00000054270.

Structurei

3D structure databases

ProteinModelPortaliP05627.
SMRiP05627.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini255 – 318bZIPPROSITE-ProRule annotationAdd BLAST64

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni255 – 282Basic motifPROSITE-ProRule annotationAdd BLAST28
Regioni283 – 311Leucine-zipperPROSITE-ProRule annotationAdd BLAST29

Sequence similaritiesi

Belongs to the bZIP family. Jun subfamily.Curated
Contains 1 bZIP (basic-leucine zipper) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0837. Eukaryota.
ENOG410XRWH. LUCA.
GeneTreeiENSGT00390000009929.
HOGENOMiHOG000006648.
HOVERGENiHBG001722.
InParanoidiP05627.
KOiK04448.
OMAiAELHNQN.
OrthoDBiEOG091G0N0L.
PhylomeDBiP05627.
TreeFamiTF323952.

Family and domain databases

Gene3Di1.10.880.10. 1 hit.
InterProiIPR004827. bZIP.
IPR015558. C_Jun/v-Jun.
IPR005643. JNK.
IPR002112. Leuzip_Jun.
IPR008917. TF_DNA-bd.
[Graphical view]
PANTHERiPTHR11462:SF8. PTHR11462:SF8. 1 hit.
PfamiPF00170. bZIP_1. 1 hit.
PF03957. Jun. 1 hit.
[Graphical view]
PRINTSiPR00043. LEUZIPPRJUN.
SMARTiSM00338. BRLZ. 1 hit.
[Graphical view]
SUPFAMiSSF47454. SSF47454. 1 hit.
PROSITEiPS50217. BZIP. 1 hit.
PS00036. BZIP_BASIC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P05627-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTAKMETTFY DDALNASFLQ SESGAYGYSN PKILKQSMTL NLADPVGSLK
60 70 80 90 100
PHLRAKNSDL LTSPDVGLLK LASPELERLI IQSSNGHITT TPTPTQFLCP
110 120 130 140 150
KNVTDEQEGF AEGFVRALAE LHSQNTLPSV TSAAQPVSGA GMVAPAVASV
160 170 180 190 200
AGAGGGGGYS ASLHSEPPVY ANLSNFNPGA LSSGGGAPSY GAAGLAFPSQ
210 220 230 240 250
PQQQQQPPQP PHHLPQQIPV QHPRLQALKE EPQTVPEMPG ETPPLSPIDM
260 270 280 290 300
ESQERIKAER KRMRNRIAAS KCRKRKLERI ARLEEKVKTL KAQNSELAST
310 320 330
ANMLREQVAQ LKQKVMNHVN SGCQLMLTQQ LQTF
Length:334
Mass (Da):35,944
Last modified:October 1, 1989 - v3
Checksum:i2BE0E4025B8B1CA3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti183S → C in CAA31252 (PubMed:3136397).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X12761 mRNA. Translation: CAA31252.1.
X12740 mRNA. Translation: CAA31236.1.
J04115 mRNA. Translation: AAA37419.1.
BC002081 mRNA. Translation: AAH02081.1.
BC021888 mRNA. Translation: AAH21888.1.
CCDSiCCDS18364.1.
PIRiA31345. TVMSJA.
RefSeqiNP_034721.1. NM_010591.2.
UniGeneiMm.275071.

Genome annotation databases

EnsembliENSMUST00000107094; ENSMUSP00000102711; ENSMUSG00000052684.
GeneIDi16476.
KEGGimmu:16476.
UCSCiuc008tsq.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X12761 mRNA. Translation: CAA31252.1.
X12740 mRNA. Translation: CAA31236.1.
J04115 mRNA. Translation: AAA37419.1.
BC002081 mRNA. Translation: AAH02081.1.
BC021888 mRNA. Translation: AAH21888.1.
CCDSiCCDS18364.1.
PIRiA31345. TVMSJA.
RefSeqiNP_034721.1. NM_010591.2.
UniGeneiMm.275071.

3D structure databases

ProteinModelPortaliP05627.
SMRiP05627.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200871. 39 interactors.
DIPiDIP-1068N.
IntActiP05627. 5 interactors.
MINTiMINT-209953.
STRINGi10090.ENSMUSP00000054270.

Chemistry databases

ChEMBLiCHEMBL5369.

PTM databases

iPTMnetiP05627.
PhosphoSitePlusiP05627.

Proteomic databases

EPDiP05627.
PaxDbiP05627.
PeptideAtlasiP05627.
PRIDEiP05627.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000107094; ENSMUSP00000102711; ENSMUSG00000052684.
GeneIDi16476.
KEGGimmu:16476.
UCSCiuc008tsq.2. mouse.

Organism-specific databases

CTDi3725.
MGIiMGI:96646. Jun.

Phylogenomic databases

eggNOGiKOG0837. Eukaryota.
ENOG410XRWH. LUCA.
GeneTreeiENSGT00390000009929.
HOGENOMiHOG000006648.
HOVERGENiHBG001722.
InParanoidiP05627.
KOiK04448.
OMAiAELHNQN.
OrthoDBiEOG091G0N0L.
PhylomeDBiP05627.
TreeFamiTF323952.

Enzyme and pathway databases

ReactomeiR-MMU-2559580. Oxidative Stress Induced Senescence.
R-MMU-2871796. FCERI mediated MAPK activation.
R-MMU-450341. Activation of the AP-1 family of transcription factors.
R-MMU-5617472. Activation of anterior HOX genes in hindbrain development during early embryogenesis.

Miscellaneous databases

PROiP05627.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000052684.
CleanExiMM_JUN.
ExpressionAtlasiP05627. baseline and differential.
GenevisibleiP05627. MM.

Family and domain databases

Gene3Di1.10.880.10. 1 hit.
InterProiIPR004827. bZIP.
IPR015558. C_Jun/v-Jun.
IPR005643. JNK.
IPR002112. Leuzip_Jun.
IPR008917. TF_DNA-bd.
[Graphical view]
PANTHERiPTHR11462:SF8. PTHR11462:SF8. 1 hit.
PfamiPF00170. bZIP_1. 1 hit.
PF03957. Jun. 1 hit.
[Graphical view]
PRINTSiPR00043. LEUZIPPRJUN.
SMARTiSM00338. BRLZ. 1 hit.
[Graphical view]
SUPFAMiSSF47454. SSF47454. 1 hit.
PROSITEiPS50217. BZIP. 1 hit.
PS00036. BZIP_BASIC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiJUN_MOUSE
AccessioniPrimary (citable) accession number: P05627
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: October 1, 1989
Last modified: November 2, 2016
This is version 172 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.