P05626 (ATPF_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP synthase subunit 4, mitochondrial

Gene names

Name:	ATP4
Ordered Locus Names:	YPL078C
ORF Names:	LPF7C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	244 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core, and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F0 domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha3beta3 subcomplex and subunit a/ATP6 static relative to the rotary elements.
Subunit structure	F-type ATPases have 2 components, CF1 - the catalytic core - and CF0 - the membrane proton channel. In yeast, the dimeric form of ATP synthase consists of 17 polypeptides: alpha, beta, gamma, delta, epsilon, 4 (B), 5 (OSCP), 6 (A), 8, 9 (C), d, E (Tim11), f, g, h, i/j and k.
Subcellular location	Mitochondrion. Mitochondrion inner membrane.
Miscellaneous	Present with 12900 molecules/cell in log phase SD medium. Ref.7
Sequence similarities	Belongs to the eukaryotic ATPase B chain family.

Ontologies

Keywords
Biological process	Hydrogen ion transport Ion transport Transport
Cellular component	CF(0) Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	ATP synthesis coupled proton transport Inferred from direct assay. Source: SGD protein complex oligomerization Inferred from mutant phenotype. Source: SGD
Cellular component	mitochondrial proton-transporting ATP synthase, stator stalk Inferred from direct assay. Source: SGD
Molecular function	hydrogen ion transmembrane transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 35	35	Mitochondrion Ref.6
	Chain	36 – 244	209	ATP synthase subunit 4, mitochondrial		PRO_0000002524
Amino acid modifications
	Modified residue	144	1	Phosphoserine Ref.8
Experimental info
	Sequence conflict	11	1	A → L in AAT92995. Ref.5
	Sequence conflict	172	1	A → R in CAA29909. Ref.1
	Sequence conflict	172	1	A → R in CAA34703. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P05626 [UniParc]. Last modified October 5, 2010. Version 2. Checksum: BDBE0F6AD6E5392C	FASTA	244	26,925
	10 20 30 40 50 60 MSMSMGVRGL ALRSVSKTLF SQGVRCPSMV IGARYMSSTP EKQTDPKAKA NSIINAIPGN 70 80 90 100 110 120 NILTKTGVLG TSAAAVIYAI SNELYVINDE SILLLTFLGF TGLVAKYLAP AYKDFADARM 130 140 150 160 170 180 KKVSDVLNAS RNKHVEAVKD RIDSVSQLQN VAETTKVLFD VSKETVELES EAFELKQKVE 190 200 210 220 230 240 LAHEAKAVLD SWVRYEASLR QLEQRQLAKS VISRVQSELG NPKFQEKVLQ QSISEIEQLL SKLK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"ATP4, the structural gene for yeast F0F1 ATPase subunit 4." Velours J., Durrens P., Aigle M., Guerin B. Eur. J. Biochem. 170:637-642(1988) [PubMed: 2892678] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The yeast ATP synthase subunit 4: structure and function." Velours J., Arselin G., Paul M.-F., Galante M., Durrens P., Aigle M., Guerin B. Biochimie 71:903-915(1989) [PubMed: 2553130] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J. Nature 387:103-105(1997) [PubMed: 9169875] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[6]	"Subunit 4 of ATP synthase (F0F1) from yeast mitochondria. Purification, amino-acid composition and partial N-terminal sequence." Velours J., Arselin de Chateaubodeau G., Galante M., Guerin B. Eur. J. Biochem. 164:579-584(1987) [PubMed: 2883007] [Abstract] Cited for: PROTEIN SEQUENCE OF 36-45.
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"Profiling phosphoproteins of yeast mitochondria reveals a role of phosphorylation in assembly of the ATP synthase." Reinders J., Wagner K., Zahedi R.P., Stojanovski D., Eyrich B., van der Laan M., Rehling P., Sickmann A., Pfanner N., Meisinger C. Mol. Cell. Proteomics 6:1896-1906(2007) [PubMed: 17761666] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-144, MASS SPECTROMETRY. Strain: ATCC 76625 / YPH499.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X06732 Genomic DNA. Translation: CAA29909.1. X16721 Genomic DNA. Translation: CAA34703.1. U41849 Genomic DNA. Translation: AAB68260.1. AY692976 Genomic DNA. Translation: AAT92995.1. BK006949 Genomic DNA. Translation: DAA11354.1.
PIR	PWBYBC. S61109.
RefSeq	NP_015247.1. NM_001183892.1.
3D structure databases
ProteinModelPortal	P05626.
SMR	P05626. Positions 196-242.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-3036N.
IntAct	P05626. 6 interactions.
MINT	MINT-674963.
STRING	P05626.
Protein family/group databases
TCDB	3.A.2.1.3. H+- or Na+-translocating F-type, V-type and A-type ATPase (F-ATPase) superfamily.
Proteomic databases
PeptideAtlas	P05626.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YPL078C; YPL078C; YPL078C.
GeneID	856027.
KEGG	sce:YPL078C.
Organism-specific databases
SGD	S000005999. ATP4.
Phylogenomic databases
eggNOG	fuNOG04322.
HOGENOM	HBG328743.
OMA	DSWVRYE.
OrthoDB	EOG451J1B.
Gene expression databases
ArrayExpress	P05626.
Genevestigator	P05626.
GermOnline	YPL078C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR008688. ATPase_F0-cplx_bsu_mt. IPR013837. ATPase_F0-cplx_bsu_mt_met. [Graphical view]
KO	K02127.
PANTHER	PTHR12733. ATPase_F0_B_mt_met. 1 hit.
Pfam	PF05405. Mt_ATP-synt_B. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ATPF_YEAST

Accession

Primary (citable) accession number: P05626
Secondary accession number(s): D6W3T8, E9P905, Q02830

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1988
Last sequence update:	October 5, 2010
Last modified:	December 14, 2011
This is version 116 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program