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P05625

- RAF1_CHICK

UniProt

P05625 - RAF1_CHICK

Protein

RAF proto-oncogene serine/threonine-protein kinase

Gene

RAF1

Organism
Gallus gallus (Chicken)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 132 (01 Oct 2014)
      Sequence version 1 (01 Nov 1988)
      Previous versions | rss
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    Functioni

    Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2) By similarity.By similarity

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei375 – 3751ATPPROSITE-ProRule annotation
    Active sitei468 – 4681Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri138 – 18447Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd
    BLAST
    Nucleotide bindingi355 – 3639ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. protein serine/threonine kinase activity Source: UniProtKB-KW
    4. receptor signaling protein activity Source: InterPro

    GO - Biological processi

    1. death-inducing signaling complex assembly Source: Ensembl
    2. intermediate filament cytoskeleton organization Source: Ensembl
    3. negative regulation of apoptotic process Source: Ensembl
    4. negative regulation of cell proliferation Source: Ensembl
    5. negative regulation of extrinsic apoptotic signaling pathway via death domain receptors Source: Ensembl
    6. negative regulation of protein complex assembly Source: Ensembl
    7. neurotrophin TRK receptor signaling pathway Source: Ensembl
    8. positive regulation of peptidyl-serine phosphorylation Source: Ensembl

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BRENDAi2.7.11.1. 1306.
    ReactomeiREACT_197481. CREB phosphorylation through the activation of Ras.
    REACT_197905. Stimuli-sensing channels.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    RAF proto-oncogene serine/threonine-protein kinase (EC:2.7.11.1)
    Alternative name(s):
    C-MIL
    C-RAF
    MIL proto-oncogene serine/threonine-protein kinase
    RAF-1
    Gene namesi
    Name:RAF1
    Synonyms:MIL
    OrganismiGallus gallus (Chicken)
    Taxonomic identifieri9031 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus
    ProteomesiUP000000539: Chromosome 12

    Subcellular locationi

    Cytoplasm By similarity. Cell membrane By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. Golgi apparatus Source: Ensembl
    3. plasma membrane Source: UniProtKB
    4. pseudopodium Source: Ensembl

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Membrane

    Pathology & Biotechi

    Keywords - Diseasei

    Proto-oncogene

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 647647RAF proto-oncogene serine/threonine-protein kinasePRO_0000086599Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei43 – 431PhosphoserineBy similarity
    Modified residuei259 – 2591PhosphoserineBy similarity
    Modified residuei268 – 2681Phosphothreonine; by autocatalysisBy similarity
    Modified residuei338 – 3381PhosphoserineBy similarity
    Modified residuei499 – 4991PhosphoserineBy similarity
    Modified residuei621 – 6211PhosphoserineBy similarity

    Post-translational modificationi

    Phosphorylation at Ser-259 inactivates kinase activity. Dephosphorylation of Ser-259 by a complex containing protein phosphatase 1 relieves inactivation, leading to stimulate RAF1 activity By similarity.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiP05625.
    PRIDEiP05625.

    Expressioni

    Tissue specificityi

    Isoform 1 was present in all tissues tested: skeletal muscle, intestine, brain, gizzard, heart, lung, kidney, bone marrow, spleen and bursa of Fabricius. Isoform 2 was only detected in brain, heart and skeletal muscle. In brain and heart isoform 1 is more abundant than isoform 2. In skeletal muscle isoform 2 is more abundant than isoform 1.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliP05625.
    SMRiP05625. Positions 55-132, 136-187, 342-615.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini56 – 13176RBDPROSITE-ProRule annotationAdd
    BLAST
    Domaini349 – 609261Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 phorbol-ester/DAG-type zinc finger.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation
    Contains 1 RBD (Ras-binding) domain.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri138 – 18447Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00750000117324.
    HOGENOMiHOG000252972.
    HOVERGENiHBG001886.
    InParanoidiP05625.
    KOiK04366.
    OMAiDGPSCIS.
    OrthoDBiEOG7F5128.
    PhylomeDBiP05625.
    TreeFamiTF317006.

    Family and domain databases

    InterProiIPR020454. DAG/PE-bd.
    IPR011009. Kinase-like_dom.
    IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR003116. Raf-like_ras-bd.
    IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
    IPR008271. Ser/Thr_kinase_AS.
    IPR029071. Ubiquitin-rel_dom.
    [Graphical view]
    PfamiPF00130. C1_1. 1 hit.
    PF07714. Pkinase_Tyr. 1 hit.
    PF02196. RBD. 1 hit.
    [Graphical view]
    PRINTSiPR00008. DAGPEDOMAIN.
    SMARTiSM00109. C1. 1 hit.
    SM00455. RBD. 1 hit.
    [Graphical view]
    SUPFAMiSSF54236. SSF54236. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    PS50898. RBD. 1 hit.
    PS00479. ZF_DAG_PE_1. 1 hit.
    PS50081. ZF_DAG_PE_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P05625-1) [UniParc]FASTAAdd to Basket

    Also known as: 6C

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MEHIQGAWKT ISNGFGLKDS VFDGPNCISP TIVQQFGYQR RASDDGKISD    50
    TSKTSNTIRV FLPNKQRTVV NVRNGMTLHD CLMKALKVRG LQPECCAVFR 100
    LVTEPKGKKV RLDWNTDAAS LIGEELQVDF LDHVPLTTHN FARKTFLKLA 150
    FCDICQKFLL NGFRCQTCGY KFHEHCSTKV PTMCVDWSNI RQLLLFPNSN 200
    ISDSGVPALP PLTMRRMRES VSRIPVSSQH RYSTPHVFTF NTSNPSSEGT 250
    LSQRQRSTST PNVHMVSTTM PVDSRIIEDA IRNHSESASP SALSGSPNNM 300
    SPTGWSQPKT PVPAQRERAP GTNTQEKNKI RPRGQRDSSY YWEIEASEVM 350
    LSTRIGSGSF GTVYKGKWHG DVAVKILKVV DPTPEQFQAF RNEVAVLRKT 400
    RHVNILLFMG YMTKDNLAIV TQWCEGSSLY KHLHVQETKF QMFQLIDIAR 450
    QTAQGMDYLH AKNIIHRDMK SNNIFLHEGL TVKIGDFGLA TVKSRWSGSQ 500
    QVEQPTGSIL WMAPEVIRMQ DSNPFSFQSD VYSYGIVLYE LMTGELPYSH 550
    INNRDQIIFM VGRGYASPDL SKLYKNCPKA MKRLVADCLK KVREERPLFP 600
    QILSSIELLQ HSLPKINRSA SEPSLHRASH TEDINSCTLT STRLPVF 647
    Length:647
    Mass (Da):73,125
    Last modified:November 1, 1988 - v1
    Checksum:i8E1443667312DFC0
    GO
    Isoform 2 (identifier: P05625-2) [UniParc]FASTAAdd to Basket

    Also known as: 1A

    The sequence of this isoform differs from the canonical sequence as follows:
         278-278: E → ENNSLNASPSSWCRRFCLRGR

    Show »
    Length:667
    Mass (Da):75,431
    Checksum:iFB1437BCD25B18EB
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti549 – 5491S → F in AAA48952. (PubMed:2998016)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei278 – 2781E → ENNSLNASPSSWCRRFCLRG R in isoform 2. 1 PublicationVSP_034628

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X07017 mRNA. Translation: CAA30069.1.
    K03269
    , K03259, K03260, K03261, K03262, K03263, K03264, K03265, K03266, K03267, K03268 Genomic DNA. Translation: AAA48952.1.
    K03048, K03047 Genomic DNA. Translation: AAA48951.1.
    M19461 mRNA. Translation: AAA48953.1.
    X55430 Genomic DNA. No translation available.
    PIRiI50382.
    S00644.
    RefSeqiNP_990638.1. NM_205307.2. [P05625-1]
    UniGeneiGga.42842.

    Genome annotation databases

    EnsembliENSGALT00000033956; ENSGALP00000033314; ENSGALG00000004998. [P05625-1]
    GeneIDi396245.
    KEGGigga:396245.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X07017 mRNA. Translation: CAA30069.1 .
    K03269
    , K03259 , K03260 , K03261 , K03262 , K03263 , K03264 , K03265 , K03266 , K03267 , K03268 Genomic DNA. Translation: AAA48952.1 .
    K03048 , K03047 Genomic DNA. Translation: AAA48951.1 .
    M19461 mRNA. Translation: AAA48953.1 .
    X55430 Genomic DNA. No translation available.
    PIRi I50382.
    S00644.
    RefSeqi NP_990638.1. NM_205307.2. [P05625-1 ]
    UniGenei Gga.42842.

    3D structure databases

    ProteinModelPortali P05625.
    SMRi P05625. Positions 55-132, 136-187, 342-615.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PaxDbi P05625.
    PRIDEi P05625.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSGALT00000033956 ; ENSGALP00000033314 ; ENSGALG00000004998 . [P05625-1 ]
    GeneIDi 396245.
    KEGGi gga:396245.

    Organism-specific databases

    CTDi 5894.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00750000117324.
    HOGENOMi HOG000252972.
    HOVERGENi HBG001886.
    InParanoidi P05625.
    KOi K04366.
    OMAi DGPSCIS.
    OrthoDBi EOG7F5128.
    PhylomeDBi P05625.
    TreeFami TF317006.

    Enzyme and pathway databases

    BRENDAi 2.7.11.1. 1306.
    Reactomei REACT_197481. CREB phosphorylation through the activation of Ras.
    REACT_197905. Stimuli-sensing channels.

    Miscellaneous databases

    NextBioi 20816297.
    PROi P05625.

    Family and domain databases

    InterProi IPR020454. DAG/PE-bd.
    IPR011009. Kinase-like_dom.
    IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR003116. Raf-like_ras-bd.
    IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
    IPR008271. Ser/Thr_kinase_AS.
    IPR029071. Ubiquitin-rel_dom.
    [Graphical view ]
    Pfami PF00130. C1_1. 1 hit.
    PF07714. Pkinase_Tyr. 1 hit.
    PF02196. RBD. 1 hit.
    [Graphical view ]
    PRINTSi PR00008. DAGPEDOMAIN.
    SMARTi SM00109. C1. 1 hit.
    SM00455. RBD. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54236. SSF54236. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    PS50898. RBD. 1 hit.
    PS00479. ZF_DAG_PE_1. 1 hit.
    PS50081. ZF_DAG_PE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Primary structure of the chicken c-mil protein: identification of domains shared with or absent from the retroviral v-mil protein."
      Koenen M., Sippel A.E., Trachmann C., Bister K.
      Oncogene 2:179-185(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Tissue: Erythroblast.
    2. "Nucleotide sequence analysis of the chicken gene c-mil, the progenitor of the retroviral oncogene v-mil."
      Jansen H.W., Bister K.
      Virology 143:359-367(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 228-647.
    3. "Analysis of the cellular proto-oncogene mht/raf: relationship to the 5' sequences of v-mht in avian carcinoma virus MH2 and v-raf in murine sarcoma virus 3611."
      Flordellis C.S., Kan N.C., Lautenberger J.A., Samuel K.P., Garon C.F., Papas T.S.
      Virology 141:267-274(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 230-330.
    4. "Alternative splicing of RNAs transcribed from the chicken c-mil gene."
      Dozier C., Denhez F., Henry C., Coll J., Begue A., Quatannens B., Saule S., Stehelin D.
      Mol. Cell. Biol. 8:1835-1838(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 275-283 (ISOFORM 2), ALTERNATIVE SPLICING.
      Tissue: Embryo.
    5. "An alternatively spliced c-mil/raf mRNA is predominantly expressed in chicken muscular tissues and conserved among vertebrate species."
      Dozier C., Ansieau S., Ferreira E., Coll J., Stehelin D.
      Oncogene 6:1307-1311(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: PARTIAL NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiRAF1_CHICK
    AccessioniPrimary (citable) accession number: P05625
    Secondary accession number(s): Q90893, Q90894
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1988
    Last sequence update: November 1, 1988
    Last modified: October 1, 2014
    This is version 132 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3