P05623 (A70A_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Accessory gland-specific peptide 70A

Alternative name(s):
Paragonial peptide B
Sex peptide
Short name=SP

Gene names

Name:	Acp70A
Synonyms:	PAPB
ORF Names:	CG17673

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	55 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Represses female sexual receptivity and stimulates oviposition.
Subcellular location	Secreted.
Tissue specificity	Main cells of the accessory glands of males (paragonial gland).

Ontologies

Keywords
Biological process	Behavior
Cellular component	Secreted
Coding sequence diversity	Polymorphism
Domain	Signal
PTM	Disulfide bond Hydroxylation
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	adult feeding behavior Inferred from mutant phenotype. Source: FlyBase juvenile hormone biosynthetic process Non-traceable author statement. Source: FlyBase negative regulation of female receptivity, post-mating Inferred from mutant phenotype. Source: FlyBase oogenesis Traceable author statement. Source: FlyBase oviposition Traceable author statement. Source: FlyBase regulation of juvenile hormone biosynthetic process Traceable author statement. Source: FlyBase regulation of post-mating oviposition Inferred from mutant phenotype. Source: FlyBase
Cellular component	extracellular region Traceable author statement. Source: FlyBase
Molecular function	hormone activity Non-traceable author statement. Source: FlyBase
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 19

19

Ref.1

Chain

20 – 55

36

Accessory gland-specific peptide 70A

PRO_0000020587

Amino acid modifications

Modified residue

28

1

Hydroxyproline Ref.1

Modified residue

32

1

Hydroxyproline

Modified residue

33

1

Isoleucine derivative

Modified residue

34

1

Hydroxyproline Ref.1

Modified residue

38

1

Hydroxyproline

Disulfide bond

43 ↔ 55

Ref.6

Natural variations

Natural variant

19

1

S → A in strain: Berkeley, M2, M26, M36, M40, M55 and SFS 3.4.

Natural variant

46

1

N → I in strain: SFS 3.4.

Secondary structure

1

.

.

.

55

Helix Strand Turn

Details...

Beta strand

32 – 35

4

Sequences

	Sequence		Length	Mass (Da)
	P05623 [UniParc]. Last modified November 1, 1988. Version 1. Checksum: F6827A1F025BF23D	FASTA	55	6,378
	10 20 30 40 50 MKTLALFLVL VCVLGLVQSW EWPWNRKPTK FPIPSPNPRD KWCRLNLGPA WGGRC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A male accessory gland peptide that regulates reproductive behavior of female D. melanogaster." Chen P.S., Stumm-Zollinger E., Aigaki T., Balmer J., Bienz M., Boehlen P. Cell 54:291-298(1988) [PubMed: 3135120] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 20-55. Strain: Oregon-R.
[2]	"Evolutionary history of the sex-peptide (Acp70A) gene region in Drosophila melanogaster." Cirera S., Aguade M.N. Genetics 147:189-197(1997) [PubMed: 9286679] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: M11, M2, M26, M36, M40, M47, M54, M55 and M66.
[3]	"Population genetics of accessory gland proteins and sexual behavior in Drosophila melanogaster populations from Evolution Canyon." Panhuis T.M., Swanson W.J., Nunney L. Evolution 57:2785-2791(2003) [PubMed: 14761057] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: NFS 5.1, NFS 5.2, NFS 5.3, NFS 5.4, NFS 6.1, NFS 6.2, NFS 6.3, NFS 6.4, NFS 7.8, SFS 1.1, SFS 1.2, SFS 1.3, SFS 1.4, SFS 2.2, SFS 2.3, SFS 2.4, SFS 3.1, SFS 3.2, SFS 3.3 and SFS 3.4.
[4]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[5]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[6]	"NMR studies of the solution conformation of the sex peptide from Drosophila melanogaster." Moehle K., Freund A., Kubli E., Robinson J.A. FEBS Lett. 585:1197-1202(2011) [PubMed: 21439282] [Abstract] Cited for: STRUCTURE BY NMR OF 20-55, DISULFIDE BOND.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M21201 mRNA. Translation: AAA28816.1.
X99407 Genomic DNA. Translation: CAA67784.1.
X99408 Genomic DNA. Translation: CAA67785.1.
X99409 Genomic DNA. Translation: CAA67786.1.
X99410 Genomic DNA. Translation: CAA67787.1.
X99411 Genomic DNA. Translation: CAA67788.1.
X99413 Genomic DNA. Translation: CAA67790.1.
X99415 Genomic DNA. Translation: CAA67792.1.
X99416 Genomic DNA. Translation: CAA67793.1.
X99418 Genomic DNA. Translation: CAA67795.1.
AY344285 Genomic DNA. Translation: AAR04600.1.
AY344286 Genomic DNA. Translation: AAR04601.1.
AY344287 Genomic DNA. Translation: AAR04602.1.
AY344288 Genomic DNA. Translation: AAR04603.1.
AY344289 Genomic DNA. Translation: AAR04604.1.
AY344290 Genomic DNA. Translation: AAR04605.1.
AY344291 Genomic DNA. Translation: AAR04606.1.
AY344292 Genomic DNA. Translation: AAR04607.1.
AY344293 Genomic DNA. Translation: AAR04608.1.
AY344294 Genomic DNA. Translation: AAR04609.1.
AY344295 Genomic DNA. Translation: AAR04610.1.
AY344296 Genomic DNA. Translation: AAR04611.1.
AY344297 Genomic DNA. Translation: AAR04612.1.
AY344298 Genomic DNA. Translation: AAR04613.1.
AY344299 Genomic DNA. Translation: AAR04614.1.
AY344300 Genomic DNA. Translation: AAR04615.1.
AY344301 Genomic DNA. Translation: AAR04616.1.
AY344302 Genomic DNA. Translation: AAR04617.1.
AY344303 Genomic DNA. Translation: AAR04618.1.
AY344304 Genomic DNA. Translation: AAR04619.1.
AE014296 Genomic DNA. Translation: AAF49836.1.

PIR

A28911.

RefSeq

NP_524057.2. NM_079333.2.

UniGene

Dm.2898.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2LAQ	NMR	-	A	20-55	[»]

ProteinModelPortal

P05623.

SMR

P05623. Positions 20-55.

ModBase

Search...

Protein-protein interaction databases

IntAct

P05623. 1 interaction.

MINT

MINT-773629.

STRING

P05623.

Proteomic databases

PRIDE

P05623.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

39494.

KEGG

dme:Dmel_CG17673.

Organism-specific databases

CTD

39494.

FlyBase

FBgn0003034. Acp70A.

Phylogenomic databases

OrthoDB

EOG40K6GQ.

Gene expression databases

Bgee

P05623.

GermOnline

CG17673. Drosophila melanogaster.

Family and domain databases

InterPro

IPR012608. Sex_peptide.
[Graphical view]

Pfam

PF08138. Sex_peptide. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

813935.

Entry information

Entry name

A70A_DROME

Accession

Primary (citable) accession number: P05623
Secondary accession number(s): O18659, Q6VBF8, Q9VU54

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1988
Last sequence update:	November 1, 1988
Last modified:	January 25, 2012
This is version 98 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project