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Protein

Integrin alpha-M

Gene

Itgam

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. Alpha-M/beta-2 play a critical role in mast cell development and in immune complex-mediated glomerulonephritis. Mice expressing a null mutation of the alpha-M subunit gene demonstrate increase in neutrophil accumulation, in response to a impaired degranulation and phagocytosis, events that apparently accelerate apoptosis in neutrophils. These mice develop obesity.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi465 – 473Sequence analysis9
Calcium bindingi529 – 537Sequence analysis9
Calcium bindingi592 – 600Sequence analysis9

GO - Molecular functioni

  • glycoprotein binding Source: MGI
  • heparan sulfate proteoglycan binding Source: MGI
  • heparin binding Source: MGI
  • metal ion binding Source: UniProtKB-KW
  • opsonin binding Source: MGI

GO - Biological processi

  • activated T cell proliferation Source: MGI
  • cell adhesion Source: MGI
  • cellular extravasation Source: MGI
  • integrin-mediated signaling pathway Source: UniProtKB-KW
  • leukocyte cell-cell adhesion Source: MGI
  • leukocyte migration involved in inflammatory response Source: MGI
  • microglia development Source: MGI
  • neutrophil chemotaxis Source: MGI
  • single organismal cell-cell adhesion Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Integrin, Receptor

Keywords - Biological processi

Cell adhesion

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Integrin alpha-M
Alternative name(s):
CD11 antigen-like family member B
CR-3 alpha chain
Cell surface glycoprotein MAC-1 subunit alpha
Leukocyte adhesion receptor MO1
CD_antigen: CD11b
Gene namesi
Name:Itgam
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:96607. Itgam.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini17 – 1105ExtracellularSequence analysisAdd BLAST1089
Transmembranei1106 – 1129HelicalSequence analysisAdd BLAST24
Topological domaini1130 – 1153CytoplasmicSequence analysisAdd BLAST24

GO - Cellular componenti

  • cell surface Source: MGI
  • external side of plasma membrane Source: MGI
  • extracellular exosome Source: MGI
  • extracellular space Source: MGI
  • integral component of membrane Source: MGI
  • integrin complex Source: InterPro
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3603.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 161 PublicationAdd BLAST16
ChainiPRO_000001629017 – 1153Integrin alpha-MAdd BLAST1137

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi58N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi66 ↔ 73By similarity
Glycosylationi86N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi105 ↔ 123By similarity
Glycosylationi391N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi654 ↔ 711By similarity
Glycosylationi696N-linked (GlcNAc...)Sequence analysis1
Glycosylationi734N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi770 ↔ 776By similarity
Glycosylationi772N-linked (GlcNAc...)Sequence analysis1
Glycosylationi801N-linked (GlcNAc...)Sequence analysis1
Glycosylationi881N-linked (GlcNAc...)Sequence analysis1
Glycosylationi907N-linked (GlcNAc...)Sequence analysis1
Glycosylationi941N-linked (GlcNAc...)Sequence analysis1
Glycosylationi980N-linked (GlcNAc...)Sequence analysis1
Glycosylationi994N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi999 ↔ 1023By similarity
Glycosylationi1022N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi1028 ↔ 1033By similarity
Glycosylationi1045N-linked (GlcNAc...)Sequence analysis1
Glycosylationi1051N-linked (GlcNAc...)Sequence analysis1
Glycosylationi1076N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiP05555.
PaxDbiP05555.
PeptideAtlasiP05555.
PRIDEiP05555.

PTM databases

PhosphoSitePlusiP05555.

Expressioni

Tissue specificityi

Predominantly expressed in monocytes and granulocytes.

Gene expression databases

BgeeiENSMUSG00000030786.
CleanExiMM_ITGAM.

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta chain. Alpha-M associates with beta-2. Interacts with JAM3. Interacts with THBD.By similarity

GO - Molecular functioni

  • opsonin binding Source: MGI

Protein-protein interaction databases

IntActiP05555. 2 interactors.
MINTiMINT-4098963.
STRINGi10090.ENSMUSP00000101849.

Structurei

3D structure databases

ProteinModelPortaliP05555.
SMRiP05555.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati18 – 75FG-GAP 1PROSITE-ProRule annotationAdd BLAST58
Repeati76 – 135FG-GAP 2PROSITE-ProRule annotationAdd BLAST60
Domaini164 – 338VWFAPROSITE-ProRule annotationAdd BLAST175
Repeati339 – 390FG-GAP 3PROSITE-ProRule annotationAdd BLAST52
Repeati391 – 442FG-GAP 4PROSITE-ProRule annotationAdd BLAST52
Repeati443 – 503FG-GAP 5PROSITE-ProRule annotationAdd BLAST61
Repeati506 – 564FG-GAP 6PROSITE-ProRule annotationAdd BLAST59
Repeati569 – 629FG-GAP 7PROSITE-ProRule annotationAdd BLAST61

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi1132 – 1136GFFKR motif5

Domaini

The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.

Sequence similaritiesi

Belongs to the integrin alpha chain family.Curated
Contains 7 FG-GAP repeats.PROSITE-ProRule annotation
Contains 1 VWFA domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IPBA. Eukaryota.
ENOG410ZFBE. LUCA.
HOGENOMiHOG000113114.
HOVERGENiHBG100530.
InParanoidiP05555.
PhylomeDBiP05555.

Family and domain databases

InterProiIPR013517. FG-GAP.
IPR013519. Int_alpha_beta-p.
IPR000413. Integrin_alpha.
IPR013649. Integrin_alpha-2.
IPR018184. Integrin_alpha_C_CS.
IPR032695. Integrin_dom.
IPR002035. VWF_A.
[Graphical view]
PfamiPF01839. FG-GAP. 1 hit.
PF00357. Integrin_alpha. 1 hit.
PF08441. Integrin_alpha2. 1 hit.
PF00092. VWA. 1 hit.
[Graphical view]
PRINTSiPR01185. INTEGRINA.
SMARTiSM00191. Int_alpha. 5 hits.
SM00327. VWA. 1 hit.
[Graphical view]
SUPFAMiSSF53300. SSF53300. 1 hit.
SSF69179. SSF69179. 3 hits.
PROSITEiPS51470. FG_GAP. 7 hits.
PS00242. INTEGRIN_ALPHA. 1 hit.
PS50234. VWFA. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P05555-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTLKALLVTA LALCHGFNLD TEHPMTFQEN AKGFGQNVVQ LGGTSVVVAA
60 70 80 90 100
PQEAKAVNQT GALYQCDYST SRCHPIPLQV PPEAVNMSLG LSLAVSTVPQ
110 120 130 140 150
QLLACGPTVH QNCKENTYVN GLCYLFGSNL LRPPQQFPEA LRECPQQESD
160 170 180 190 200
IVFLIDGSGS INNIDFQKMK EFVSTVMEQF KKSKTLFSLM QYSDEFRIHF
210 220 230 240 250
TFNDFKRNPS PRSHVSPIKQ LNGRTKTASG IRKVVRELFH KTNGARENAA
260 270 280 290 300
KILVVITDGE KFGDPLDYKD VIPEADRAGV IRYVIGVGNA FNKPQSRREL
310 320 330 340 350
DTIASKPAGE HVFQVDNFEA LNTIQNQLQE KIFAIEGTQT GSTSSFEHEM
360 370 380 390 400
SQEGFSASIT SNGPLLGSVG SFDWAGGAFL YTSKDKVTFI NTTRVDSDMN
410 420 430 440 450
DAYLGYASAV ILRNRVQSLV LGAPRYQHIG LVVMFRENFG TWEPHTSIKG
460 470 480 490 500
SQIGSYFGAS LCSVDMDADG NTNLILIGAP HYYEKTRGGQ VSVCPLPRGR
510 520 530 540 550
ARWQCEALLH GDQGHPWGRF GAALTVLGDV NGDKLTDVAI GAPGEQENQG
560 570 580 590 600
AVYIFYGASI ASLSASHSHR IIGAHFSPGL QYFGQSLSGG KDLTMDGLMD
610 620 630 640 650
LAVGAQGHLL LLRAQPVLRL EATMEFSPKK VARSVFACQE QVLKNKDAGE
660 670 680 690 700
VRVCLRVRKN TKDRLREGDI QSTVTYDLAL DPVRSRIRAF FDETKNNTRR
710 720 730 740 750
RTQVFGLMQK CETLKLILPD CVDDSVSPII LRLNYTLVGE PLRSFGNLRP
760 770 780 790 800
VLAMDAQRFF TAMFPFEKNC GNDSICQDDL SITMSAMGLD TLVVGGPQDF
810 820 830 840 850
NMSVTLRNDG EDSYGTQVTV YYPSGLSYRK DSASQNPLTK KPWFVKPAES
860 870 880 890 900
SSSSEGHGAL KSTTWNINHP IFPANSEVTF NVTFDVDSHA SFGNKLLLKA
910 920 930 940 950
IVASENNMSR THKTKFQLEL PVKYAIYMIV TSDESSIRYL NFTASEMTSK
960 970 980 990 1000
VIQHQYQFNN LGQRSLPVSV VFWIPVQINN VTVWDHPQVI FSQNLSSACH
1010 1020 1030 1040 1050
TEQKSPPHSN FRDQLERTPV LNCSVAVCKR IQCDLPSFNT QEIFNVTLKG
1060 1070 1080 1090 1100
NLSFDWYIKT SHGHLLLVSS TEILFNDSAF ALLPGQESYV RSKTETKVEP
1110 1120 1130 1140 1150
YEVHNPVPLI VGSSIGGLVL LALITAGLYK LGFFKRQYKD MMNEAAPQDA

PPQ
Length:1,153
Mass (Da):127,481
Last modified:February 1, 1991 - v2
Checksum:i178DB988AECB0343
GO
Isoform 2 (identifier: P05555-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     453-569: Missing.

Note: No experimental confirmation available.
Show »
Length:1,036
Mass (Da):115,196
Checksum:i489843932CDBA7D2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti37N → S in BAC30350 (PubMed:16141072).Curated1
Sequence conflicti683V → G in BAC30350 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_010473453 – 569Missing in isoform 2. 1 PublicationAdd BLAST117

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07640 mRNA. Translation: CAA30479.1.
AK039444 mRNA. Translation: BAC30350.1.
M14293 Genomic DNA. Translation: AAA39484.1.
CCDSiCCDS21889.1. [P05555-1]
PIRiS00551.
UniGeneiMm.262106.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07640 mRNA. Translation: CAA30479.1.
AK039444 mRNA. Translation: BAC30350.1.
M14293 Genomic DNA. Translation: AAA39484.1.
CCDSiCCDS21889.1. [P05555-1]
PIRiS00551.
UniGeneiMm.262106.

3D structure databases

ProteinModelPortaliP05555.
SMRiP05555.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP05555. 2 interactors.
MINTiMINT-4098963.
STRINGi10090.ENSMUSP00000101849.

Chemistry databases

ChEMBLiCHEMBL3603.

PTM databases

PhosphoSitePlusiP05555.

Proteomic databases

MaxQBiP05555.
PaxDbiP05555.
PeptideAtlasiP05555.
PRIDEiP05555.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:96607. Itgam.

Phylogenomic databases

eggNOGiENOG410IPBA. Eukaryota.
ENOG410ZFBE. LUCA.
HOGENOMiHOG000113114.
HOVERGENiHBG100530.
InParanoidiP05555.
PhylomeDBiP05555.

Miscellaneous databases

ChiTaRSiItgam. mouse.
PROiP05555.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000030786.
CleanExiMM_ITGAM.

Family and domain databases

InterProiIPR013517. FG-GAP.
IPR013519. Int_alpha_beta-p.
IPR000413. Integrin_alpha.
IPR013649. Integrin_alpha-2.
IPR018184. Integrin_alpha_C_CS.
IPR032695. Integrin_dom.
IPR002035. VWF_A.
[Graphical view]
PfamiPF01839. FG-GAP. 1 hit.
PF00357. Integrin_alpha. 1 hit.
PF08441. Integrin_alpha2. 1 hit.
PF00092. VWA. 1 hit.
[Graphical view]
PRINTSiPR01185. INTEGRINA.
SMARTiSM00191. Int_alpha. 5 hits.
SM00327. VWA. 1 hit.
[Graphical view]
SUPFAMiSSF53300. SSF53300. 1 hit.
SSF69179. SSF69179. 3 hits.
PROSITEiPS51470. FG_GAP. 7 hits.
PS00242. INTEGRIN_ALPHA. 1 hit.
PS50234. VWFA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiITAM_MOUSE
AccessioniPrimary (citable) accession number: P05555
Secondary accession number(s): Q8CA73
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: February 1, 1991
Last modified: November 2, 2016
This is version 155 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.