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P05555

- ITAM_MOUSE

UniProt

P05555 - ITAM_MOUSE

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Protein

Integrin alpha-M

Gene
Itgam
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. Alpha-M/beta-2 play a critical role in mast cell development and in immune complex-mediated glomerulonephritis. Mice expressing a null mutation of the alpha-M subunit gene demonstrate increase in neutrophil accumulation, in response to a impaired degranulation and phagocytosis, events that apparently accelerate apoptosis in neutrophils. These mice develop obesity.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi465 – 4739 Reviewed prediction
Calcium bindingi529 – 5379 Reviewed prediction
Calcium bindingi592 – 6009 Reviewed prediction

GO - Molecular functioni

  1. heparan sulfate proteoglycan binding Source: MGI
  2. heparin binding Source: MGI
  3. metal ion binding Source: UniProtKB-KW
  4. opsonin binding Source: MGI

GO - Biological processi

  1. activated T cell proliferation Source: MGI
  2. cell adhesion Source: MGI
  3. cellular extravasation Source: MGI
  4. integrin-mediated signaling pathway Source: UniProtKB-KW
  5. leukocyte cell-cell adhesion Source: MGI
  6. leukocyte migration involved in inflammatory response Source: MGI
  7. microglia development Source: MGI
  8. neutrophil chemotaxis Source: MGI
  9. single organismal cell-cell adhesion Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Integrin, Receptor

Keywords - Biological processi

Cell adhesion

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_216309. Integrin cell surface interactions.
REACT_225233. Cell surface interactions at the vascular wall.

Names & Taxonomyi

Protein namesi
Recommended name:
Integrin alpha-M
Alternative name(s):
CD11 antigen-like family member B
CR-3 alpha chain
Cell surface glycoprotein MAC-1 subunit alpha
Leukocyte adhesion receptor MO1
CD_antigen: CD11b
Gene namesi
Name:Itgam
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 7

Organism-specific databases

MGIiMGI:96607. Itgam.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini17 – 11051089Extracellular Reviewed predictionAdd
BLAST
Transmembranei1106 – 112924Helical; Reviewed predictionAdd
BLAST
Topological domaini1130 – 115324Cytoplasmic Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. cell surface Source: MGI
  2. external side of plasma membrane Source: MGI
  3. integral component of membrane Source: MGI
  4. integrin complex Source: InterPro
  5. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 16161 PublicationAdd
BLAST
Chaini17 – 11531137Integrin alpha-MPRO_0000016290Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi58 – 581N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi66 ↔ 73 By similarity
Glycosylationi86 – 861N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi105 ↔ 123 By similarity
Glycosylationi391 – 3911N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi654 ↔ 711 By similarity
Glycosylationi696 – 6961N-linked (GlcNAc...) Reviewed prediction
Glycosylationi734 – 7341N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi770 ↔ 776 By similarity
Glycosylationi772 – 7721N-linked (GlcNAc...) Reviewed prediction
Glycosylationi801 – 8011N-linked (GlcNAc...) Reviewed prediction
Glycosylationi881 – 8811N-linked (GlcNAc...) Reviewed prediction
Glycosylationi907 – 9071N-linked (GlcNAc...) Reviewed prediction
Glycosylationi941 – 9411N-linked (GlcNAc...) Reviewed prediction
Glycosylationi980 – 9801N-linked (GlcNAc...) Reviewed prediction
Glycosylationi994 – 9941N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi999 ↔ 1023 By similarity
Glycosylationi1022 – 10221N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi1028 ↔ 1033 By similarity
Glycosylationi1045 – 10451N-linked (GlcNAc...) Reviewed prediction
Glycosylationi1051 – 10511N-linked (GlcNAc...) Reviewed prediction
Glycosylationi1076 – 10761N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP05555.
PRIDEiP05555.

PTM databases

PhosphoSiteiP05555.

Expressioni

Tissue specificityi

Predominantly expressed in monocytes and granulocytes.

Gene expression databases

ArrayExpressiP05555.
BgeeiP05555.
CleanExiMM_ITGAM.
GenevestigatoriP05555.

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta chain. Alpha-M associates with beta-2. Interacts with JAM3 By similarity.

Protein-protein interaction databases

IntActiP05555. 2 interactions.
MINTiMINT-4098963.

Structurei

3D structure databases

ProteinModelPortaliP05555.
SMRiP05555. Positions 17-146, 149-337, 344-1144.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati18 – 7558FG-GAP 1Add
BLAST
Repeati76 – 13560FG-GAP 2Add
BLAST
Domaini164 – 338175VWFAAdd
BLAST
Repeati339 – 39052FG-GAP 3Add
BLAST
Repeati391 – 44454FG-GAP 4Add
BLAST
Repeati445 – 50359FG-GAP 5Add
BLAST
Repeati506 – 56459FG-GAP 6Add
BLAST
Repeati569 – 62961FG-GAP 7Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi1132 – 11365GFFKR motif

Domaini

The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.

Sequence similaritiesi

Contains 7 FG-GAP repeats.
Contains 1 VWFA domain.

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG301393.
GeneTreeiENSGT00750000117471.
HOGENOMiHOG000113114.
HOVERGENiHBG100530.
PhylomeDBiP05555.

Family and domain databases

InterProiIPR013517. FG-GAP.
IPR013519. Int_alpha_beta-p.
IPR000413. Integrin_alpha.
IPR013649. Integrin_alpha-2.
IPR018184. Integrin_alpha_C_CS.
IPR002035. VWF_A.
[Graphical view]
PfamiPF01839. FG-GAP. 1 hit.
PF00357. Integrin_alpha. 1 hit.
PF08441. Integrin_alpha2. 1 hit.
PF00092. VWA. 1 hit.
[Graphical view]
PRINTSiPR01185. INTEGRINA.
SMARTiSM00191. Int_alpha. 5 hits.
SM00327. VWA. 1 hit.
[Graphical view]
SUPFAMiSSF53300. SSF53300. 1 hit.
PROSITEiPS51470. FG_GAP. 7 hits.
PS00242. INTEGRIN_ALPHA. 1 hit.
PS50234. VWFA. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P05555-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MTLKALLVTA LALCHGFNLD TEHPMTFQEN AKGFGQNVVQ LGGTSVVVAA     50
PQEAKAVNQT GALYQCDYST SRCHPIPLQV PPEAVNMSLG LSLAVSTVPQ 100
QLLACGPTVH QNCKENTYVN GLCYLFGSNL LRPPQQFPEA LRECPQQESD 150
IVFLIDGSGS INNIDFQKMK EFVSTVMEQF KKSKTLFSLM QYSDEFRIHF 200
TFNDFKRNPS PRSHVSPIKQ LNGRTKTASG IRKVVRELFH KTNGARENAA 250
KILVVITDGE KFGDPLDYKD VIPEADRAGV IRYVIGVGNA FNKPQSRREL 300
DTIASKPAGE HVFQVDNFEA LNTIQNQLQE KIFAIEGTQT GSTSSFEHEM 350
SQEGFSASIT SNGPLLGSVG SFDWAGGAFL YTSKDKVTFI NTTRVDSDMN 400
DAYLGYASAV ILRNRVQSLV LGAPRYQHIG LVVMFRENFG TWEPHTSIKG 450
SQIGSYFGAS LCSVDMDADG NTNLILIGAP HYYEKTRGGQ VSVCPLPRGR 500
ARWQCEALLH GDQGHPWGRF GAALTVLGDV NGDKLTDVAI GAPGEQENQG 550
AVYIFYGASI ASLSASHSHR IIGAHFSPGL QYFGQSLSGG KDLTMDGLMD 600
LAVGAQGHLL LLRAQPVLRL EATMEFSPKK VARSVFACQE QVLKNKDAGE 650
VRVCLRVRKN TKDRLREGDI QSTVTYDLAL DPVRSRIRAF FDETKNNTRR 700
RTQVFGLMQK CETLKLILPD CVDDSVSPII LRLNYTLVGE PLRSFGNLRP 750
VLAMDAQRFF TAMFPFEKNC GNDSICQDDL SITMSAMGLD TLVVGGPQDF 800
NMSVTLRNDG EDSYGTQVTV YYPSGLSYRK DSASQNPLTK KPWFVKPAES 850
SSSSEGHGAL KSTTWNINHP IFPANSEVTF NVTFDVDSHA SFGNKLLLKA 900
IVASENNMSR THKTKFQLEL PVKYAIYMIV TSDESSIRYL NFTASEMTSK 950
VIQHQYQFNN LGQRSLPVSV VFWIPVQINN VTVWDHPQVI FSQNLSSACH 1000
TEQKSPPHSN FRDQLERTPV LNCSVAVCKR IQCDLPSFNT QEIFNVTLKG 1050
NLSFDWYIKT SHGHLLLVSS TEILFNDSAF ALLPGQESYV RSKTETKVEP 1100
YEVHNPVPLI VGSSIGGLVL LALITAGLYK LGFFKRQYKD MMNEAAPQDA 1150
PPQ 1153
Length:1,153
Mass (Da):127,481
Last modified:February 1, 1991 - v2
Checksum:i178DB988AECB0343
GO
Isoform 2 (identifier: P05555-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     453-569: Missing.

Note: No experimental confirmation available.

Show »
Length:1,036
Mass (Da):115,196
Checksum:i489843932CDBA7D2
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei453 – 569117Missing in isoform 2. VSP_010473Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti37 – 371N → S in BAC30350. 1 Publication
Sequence conflicti683 – 6831V → G in BAC30350. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X07640 mRNA. Translation: CAA30479.1.
AK039444 mRNA. Translation: BAC30350.1.
M14293 Genomic DNA. Translation: AAA39484.1.
CCDSiCCDS21889.1. [P05555-1]
PIRiS00551.
UniGeneiMm.262106.

Genome annotation databases

EnsembliENSMUST00000106240; ENSMUSP00000101847; ENSMUSG00000030786.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X07640 mRNA. Translation: CAA30479.1 .
AK039444 mRNA. Translation: BAC30350.1 .
M14293 Genomic DNA. Translation: AAA39484.1 .
CCDSi CCDS21889.1. [P05555-1 ]
PIRi S00551.
UniGenei Mm.262106.

3D structure databases

ProteinModelPortali P05555.
SMRi P05555. Positions 17-146, 149-337, 344-1144.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P05555. 2 interactions.
MINTi MINT-4098963.

Chemistry

BindingDBi P05555.
ChEMBLi CHEMBL3603.

PTM databases

PhosphoSitei P05555.

Proteomic databases

PaxDbi P05555.
PRIDEi P05555.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000106240 ; ENSMUSP00000101847 ; ENSMUSG00000030786 .

Organism-specific databases

MGIi MGI:96607. Itgam.

Phylogenomic databases

eggNOGi NOG301393.
GeneTreei ENSGT00750000117471.
HOGENOMi HOG000113114.
HOVERGENi HBG100530.
PhylomeDBi P05555.

Enzyme and pathway databases

Reactomei REACT_216309. Integrin cell surface interactions.
REACT_225233. Cell surface interactions at the vascular wall.

Miscellaneous databases

ChiTaRSi ITGAM. mouse.
PROi P05555.
SOURCEi Search...

Gene expression databases

ArrayExpressi P05555.
Bgeei P05555.
CleanExi MM_ITGAM.
Genevestigatori P05555.

Family and domain databases

InterProi IPR013517. FG-GAP.
IPR013519. Int_alpha_beta-p.
IPR000413. Integrin_alpha.
IPR013649. Integrin_alpha-2.
IPR018184. Integrin_alpha_C_CS.
IPR002035. VWF_A.
[Graphical view ]
Pfami PF01839. FG-GAP. 1 hit.
PF00357. Integrin_alpha. 1 hit.
PF08441. Integrin_alpha2. 1 hit.
PF00092. VWA. 1 hit.
[Graphical view ]
PRINTSi PR01185. INTEGRINA.
SMARTi SM00191. Int_alpha. 5 hits.
SM00327. VWA. 1 hit.
[Graphical view ]
SUPFAMi SSF53300. SSF53300. 1 hit.
PROSITEi PS51470. FG_GAP. 7 hits.
PS00242. INTEGRIN_ALPHA. 1 hit.
PS50234. VWFA. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Amino acid sequence of the murine Mac-1 alpha chain reveals homology with the integrin family and an additional domain related to von Willebrand factor."
    Pytela R.
    EMBO J. 7:1371-1378(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6J.
    Tissue: Spinal cord.
  3. "A partial genomic DNA clone for the alpha subunit of the mouse complement receptor type 3 and cellular adhesion molecule Mac-1."
    Sastre L., Roman J.M., Teplow D.B., Dreyer W.J., Gee C.E., Larson R.S., Roberts T.M., Springer T.A.
    Proc. Natl. Acad. Sci. U.S.A. 83:5644-5648(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 11-45.
    Strain: BALB/c.
    Tissue: Spleen.
  4. "Sequence homology of the LFA-1 and Mac-1 leukocyte adhesion glycoproteins and unexpected relation to leukocyte interferon."
    Springer T.A., Teplow D.B., Dreyer W.J.
    Nature 314:540-542(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 17-28.

Entry informationi

Entry nameiITAM_MOUSE
AccessioniPrimary (citable) accession number: P05555
Secondary accession number(s): Q8CA73
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: February 1, 1991
Last modified: September 3, 2014
This is version 135 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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