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P05555 (ITAM_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 134. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Integrin alpha-M
Alternative name(s):
CD11 antigen-like family member B
CR-3 alpha chain
Cell surface glycoprotein MAC-1 subunit alpha
Leukocyte adhesion receptor MO1
CD_antigen=CD11b
Gene names
Name:Itgam
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1153 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. Alpha-M/beta-2 play a critical role in mast cell development and in immune complex-mediated glomerulonephritis. Mice expressing a null mutation of the alpha-M subunit gene demonstrate increase in neutrophil accumulation, in response to a impaired degranulation and phagocytosis, events that apparently accelerate apoptosis in neutrophils. These mice develop obesity.

Subunit structure

Heterodimer of an alpha and a beta chain. Alpha-M associates with beta-2. Interacts with JAM3 By similarity.

Subcellular location

Membrane; Single-pass type I membrane protein.

Tissue specificity

Predominantly expressed in monocytes and granulocytes.

Domain

The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.

Sequence similarities

Belongs to the integrin alpha chain family.

Contains 7 FG-GAP repeats.

Contains 1 VWFA domain.

Ontologies

Keywords
   Biological processCell adhesion
   Cellular componentMembrane
   Coding sequence diversityAlternative splicing
   DomainRepeat
Signal
Transmembrane
Transmembrane helix
   LigandCalcium
Metal-binding
   Molecular functionIntegrin
Receptor
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processactivated T cell proliferation

Inferred from mutant phenotype PubMed 15210787. Source: MGI

cell adhesion

Inferred from mutant phenotype PubMed 10528208. Source: MGI

cellular extravasation

Inferred from mutant phenotype PubMed 10528208. Source: MGI

integrin-mediated signaling pathway

Inferred from electronic annotation. Source: UniProtKB-KW

leukocyte cell-cell adhesion

Inferred from mutant phenotype PubMed 8562500. Source: MGI

leukocyte migration involved in inflammatory response

Inferred from mutant phenotype PubMed 23550035. Source: MGI

microglia development

Inferred from direct assay PubMed 20578039. Source: MGI

neutrophil chemotaxis

Inferred from mutant phenotype PubMed 10528208. Source: MGI

single organismal cell-cell adhesion

Inferred from genetic interaction PubMed 16093349. Source: MGI

   Cellular_componentcell surface

Inferred from direct assay PubMed 9324354. Source: MGI

external side of plasma membrane

Inferred from direct assay PubMed 10848813PubMed 12163569PubMed 14609575PubMed 15795238PubMed 15845452PubMed 16973387PubMed 19015308PubMed 19723499PubMed 20228271PubMed 20660734PubMed 24029230PubMed 8043862PubMed 8552190PubMed 8557754PubMed 8769481PubMed 8788039. Source: MGI

integral component of membrane

Inferred from direct assay PubMed 12529407. Source: MGI

integrin complex

Inferred from electronic annotation. Source: InterPro

nucleus

Inferred from direct assay PubMed 15138196. Source: MGI

   Molecular_functionheparan sulfate proteoglycan binding

Inferred from direct assay PubMed 8043862. Source: MGI

heparin binding

Inferred from direct assay PubMed 8043862. Source: MGI

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

opsonin binding

Inferred from direct assay PubMed 12163569. Source: MGI

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P05555-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P05555-2)

The sequence of this isoform differs from the canonical sequence as follows:
     453-569: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1616 Ref.4
Chain17 – 11531137Integrin alpha-M
PRO_0000016290

Regions

Topological domain17 – 11051089Extracellular Potential
Transmembrane1106 – 112924Helical; Potential
Topological domain1130 – 115324Cytoplasmic Potential
Repeat18 – 7558FG-GAP 1
Repeat76 – 13560FG-GAP 2
Domain164 – 338175VWFA
Repeat339 – 39052FG-GAP 3
Repeat391 – 44454FG-GAP 4
Repeat445 – 50359FG-GAP 5
Repeat506 – 56459FG-GAP 6
Repeat569 – 62961FG-GAP 7
Calcium binding465 – 4739 Potential
Calcium binding529 – 5379 Potential
Calcium binding592 – 6009 Potential
Motif1132 – 11365GFFKR motif

Amino acid modifications

Glycosylation581N-linked (GlcNAc...) Potential
Glycosylation861N-linked (GlcNAc...) Potential
Glycosylation3911N-linked (GlcNAc...) Potential
Glycosylation6961N-linked (GlcNAc...) Potential
Glycosylation7341N-linked (GlcNAc...) Potential
Glycosylation7721N-linked (GlcNAc...) Potential
Glycosylation8011N-linked (GlcNAc...) Potential
Glycosylation8811N-linked (GlcNAc...) Potential
Glycosylation9071N-linked (GlcNAc...) Potential
Glycosylation9411N-linked (GlcNAc...) Potential
Glycosylation9801N-linked (GlcNAc...) Potential
Glycosylation9941N-linked (GlcNAc...) Potential
Glycosylation10221N-linked (GlcNAc...) Potential
Glycosylation10451N-linked (GlcNAc...) Potential
Glycosylation10511N-linked (GlcNAc...) Potential
Glycosylation10761N-linked (GlcNAc...) Potential
Disulfide bond66 ↔ 73 By similarity
Disulfide bond105 ↔ 123 By similarity
Disulfide bond654 ↔ 711 By similarity
Disulfide bond770 ↔ 776 By similarity
Disulfide bond999 ↔ 1023 By similarity
Disulfide bond1028 ↔ 1033 By similarity

Natural variations

Alternative sequence453 – 569117Missing in isoform 2.
VSP_010473

Experimental info

Sequence conflict371N → S in BAC30350. Ref.2
Sequence conflict6831V → G in BAC30350. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified February 1, 1991. Version 2.
Checksum: 178DB988AECB0343

FASTA1,153127,481
        10         20         30         40         50         60 
MTLKALLVTA LALCHGFNLD TEHPMTFQEN AKGFGQNVVQ LGGTSVVVAA PQEAKAVNQT 

        70         80         90        100        110        120 
GALYQCDYST SRCHPIPLQV PPEAVNMSLG LSLAVSTVPQ QLLACGPTVH QNCKENTYVN 

       130        140        150        160        170        180 
GLCYLFGSNL LRPPQQFPEA LRECPQQESD IVFLIDGSGS INNIDFQKMK EFVSTVMEQF 

       190        200        210        220        230        240 
KKSKTLFSLM QYSDEFRIHF TFNDFKRNPS PRSHVSPIKQ LNGRTKTASG IRKVVRELFH 

       250        260        270        280        290        300 
KTNGARENAA KILVVITDGE KFGDPLDYKD VIPEADRAGV IRYVIGVGNA FNKPQSRREL 

       310        320        330        340        350        360 
DTIASKPAGE HVFQVDNFEA LNTIQNQLQE KIFAIEGTQT GSTSSFEHEM SQEGFSASIT 

       370        380        390        400        410        420 
SNGPLLGSVG SFDWAGGAFL YTSKDKVTFI NTTRVDSDMN DAYLGYASAV ILRNRVQSLV 

       430        440        450        460        470        480 
LGAPRYQHIG LVVMFRENFG TWEPHTSIKG SQIGSYFGAS LCSVDMDADG NTNLILIGAP 

       490        500        510        520        530        540 
HYYEKTRGGQ VSVCPLPRGR ARWQCEALLH GDQGHPWGRF GAALTVLGDV NGDKLTDVAI 

       550        560        570        580        590        600 
GAPGEQENQG AVYIFYGASI ASLSASHSHR IIGAHFSPGL QYFGQSLSGG KDLTMDGLMD 

       610        620        630        640        650        660 
LAVGAQGHLL LLRAQPVLRL EATMEFSPKK VARSVFACQE QVLKNKDAGE VRVCLRVRKN 

       670        680        690        700        710        720 
TKDRLREGDI QSTVTYDLAL DPVRSRIRAF FDETKNNTRR RTQVFGLMQK CETLKLILPD 

       730        740        750        760        770        780 
CVDDSVSPII LRLNYTLVGE PLRSFGNLRP VLAMDAQRFF TAMFPFEKNC GNDSICQDDL 

       790        800        810        820        830        840 
SITMSAMGLD TLVVGGPQDF NMSVTLRNDG EDSYGTQVTV YYPSGLSYRK DSASQNPLTK 

       850        860        870        880        890        900 
KPWFVKPAES SSSSEGHGAL KSTTWNINHP IFPANSEVTF NVTFDVDSHA SFGNKLLLKA 

       910        920        930        940        950        960 
IVASENNMSR THKTKFQLEL PVKYAIYMIV TSDESSIRYL NFTASEMTSK VIQHQYQFNN 

       970        980        990       1000       1010       1020 
LGQRSLPVSV VFWIPVQINN VTVWDHPQVI FSQNLSSACH TEQKSPPHSN FRDQLERTPV 

      1030       1040       1050       1060       1070       1080 
LNCSVAVCKR IQCDLPSFNT QEIFNVTLKG NLSFDWYIKT SHGHLLLVSS TEILFNDSAF 

      1090       1100       1110       1120       1130       1140 
ALLPGQESYV RSKTETKVEP YEVHNPVPLI VGSSIGGLVL LALITAGLYK LGFFKRQYKD 

      1150 
MMNEAAPQDA PPQ 

« Hide

Isoform 2 [UniParc].

Checksum: 489843932CDBA7D2
Show »

FASTA1,036115,196

References

« Hide 'large scale' references
[1]"Amino acid sequence of the murine Mac-1 alpha chain reveals homology with the integrin family and an additional domain related to von Willebrand factor."
Pytela R.
EMBO J. 7:1371-1378(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: C57BL/6J.
Tissue: Spinal cord.
[3]"A partial genomic DNA clone for the alpha subunit of the mouse complement receptor type 3 and cellular adhesion molecule Mac-1."
Sastre L., Roman J.M., Teplow D.B., Dreyer W.J., Gee C.E., Larson R.S., Roberts T.M., Springer T.A.
Proc. Natl. Acad. Sci. U.S.A. 83:5644-5648(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 11-45.
Strain: BALB/c.
Tissue: Spleen.
[4]"Sequence homology of the LFA-1 and Mac-1 leukocyte adhesion glycoproteins and unexpected relation to leukocyte interferon."
Springer T.A., Teplow D.B., Dreyer W.J.
Nature 314:540-542(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 17-28.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X07640 mRNA. Translation: CAA30479.1.
AK039444 mRNA. Translation: BAC30350.1.
M14293 Genomic DNA. Translation: AAA39484.1.
CCDSCCDS21889.1. [P05555-1]
PIRS00551.
UniGeneMm.262106.

3D structure databases

ProteinModelPortalP05555.
SMRP05555. Positions 17-146, 149-337, 344-1144.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP05555. 2 interactions.
MINTMINT-4098963.

Chemistry

BindingDBP05555.
ChEMBLCHEMBL3603.

PTM databases

PhosphoSiteP05555.

Proteomic databases

PaxDbP05555.
PRIDEP05555.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000106240; ENSMUSP00000101847; ENSMUSG00000030786.

Organism-specific databases

MGIMGI:96607. Itgam.

Phylogenomic databases

eggNOGNOG301393.
GeneTreeENSGT00750000117471.
HOGENOMHOG000113114.
HOVERGENHBG100530.
PhylomeDBP05555.

Gene expression databases

ArrayExpressP05555.
BgeeP05555.
CleanExMM_ITGAM.
GenevestigatorP05555.

Family and domain databases

InterProIPR013517. FG-GAP.
IPR013519. Int_alpha_beta-p.
IPR000413. Integrin_alpha.
IPR013649. Integrin_alpha-2.
IPR018184. Integrin_alpha_C_CS.
IPR002035. VWF_A.
[Graphical view]
PfamPF01839. FG-GAP. 1 hit.
PF00357. Integrin_alpha. 1 hit.
PF08441. Integrin_alpha2. 1 hit.
PF00092. VWA. 1 hit.
[Graphical view]
PRINTSPR01185. INTEGRINA.
SMARTSM00191. Int_alpha. 5 hits.
SM00327. VWA. 1 hit.
[Graphical view]
SUPFAMSSF53300. SSF53300. 1 hit.
PROSITEPS51470. FG_GAP. 7 hits.
PS00242. INTEGRIN_ALPHA. 1 hit.
PS50234. VWFA. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSITGAM. mouse.
PROP05555.
SOURCESearch...

Entry information

Entry nameITAM_MOUSE
AccessionPrimary (citable) accession number: P05555
Secondary accession number(s): Q8CA73
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: February 1, 1991
Last modified: July 9, 2014
This is version 134 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot