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Protein

T-cell surface glycoprotein CD8 beta chain

Gene

Cd8b

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. A palmitoylation site in the cytoplasmic tail of CD8B chain contributes to partitioning of CD8 into the plasma membrane lipid rafts where signaling proteins are enriched. Once LCK recruited, it initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of cytotoxic T-lymphocytes (CTLs). Additionally, plays a critical role in thymic selection of CD8+ T-cells.By similarity

GO - Biological processi

Keywordsi

Biological processAdaptive immunity, Immunity

Names & Taxonomyi

Protein namesi
Recommended name:
T-cell surface glycoprotein CD8 beta chain
Alternative name(s):
CD8 antigen 37 kDa chain
OX-8 membrane antigen
CD_antigen: CD8b
Gene namesi
Name:Cd8b
Synonyms:Cd8b1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2317. Cd8b.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini22 – 168ExtracellularSequence analysisAdd BLAST147
Transmembranei169 – 189HelicalSequence analysisAdd BLAST21
Topological domaini190 – 208CytoplasmicSequence analysisAdd BLAST19

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Add BLAST21
ChainiPRO_000001464622 – 208T-cell surface glycoprotein CD8 beta chainAdd BLAST187

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi34N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi41 ↔ 115PROSITE-ProRule annotation
Glycosylationi88N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi94N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

Phosphorylated as a consequence of T-cell activation.By similarity
Palmitoylated at the cytoplasmic tail and thereby targets the heterodimer CD8A/CD8B to lipid rafts unlike CD8A homodimers.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

Proteomic databases

PaxDbiP05541.

Interactioni

Subunit structurei

Forms disulfide-linked heterodimers with CD8A at the cell surface. Interacts with CD3D; this interaction couples TCR-CD3 with CD8. Interacts with LCK.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000009392.

Structurei

3D structure databases

ProteinModelPortaliP05541.
SMRiP05541.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini22 – 131Ig-like V-typeAdd BLAST110

Keywords - Domaini

Immunoglobulin domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IY5X. Eukaryota.
ENOG410ZF37. LUCA.
HOGENOMiHOG000008681.
HOVERGENiHBG105700.
InParanoidiP05541.
KOiK06459.
PhylomeDBiP05541.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
InterProiView protein in InterPro
IPR007110. Ig-like_dom.
IPR036179. Ig-like_dom_sf.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
PfamiView protein in Pfam
PF07686. V-set. 1 hit.
SMARTiView protein in SMART
SM00409. IG. 1 hit.
SM00406. IGv. 1 hit.
SUPFAMiSSF48726. SSF48726. 1 hit.
PROSITEiView protein in PROSITE
PS50835. IG_LIKE. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P05541-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQPWLWLVFS VKLSALWGSS ALLQTPSSLL VQTNQTAKMS CEAKTFPKGT
60 70 80 90 100
TIYWLRELQD SNKNKHFEFL ASRTSTKGIK YGERVKKNMT LSFNSTLPFL
110 120 130 140 150
KIMDVKPEDS GFYFCAMVGS PMVVFGTGTK LTVVDVLPTT APTKKTTLKK
160 170 180 190 200
KQCPTPHPKT QKGLTCGLIT LSLLVACILV LLVSLSVAIH FHCMRRRARI

HFMKQFHK
Length:208
Mass (Da):23,400
Last modified:November 1, 1988 - v1
Checksum:i1A2DA164DFA1B1F2
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti201H → R. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04310 mRNA. Translation: CAA27850.1.
PIRiA24184.
RefSeqiNP_113727.1. NM_031539.1.
UniGeneiRn.10330.

Genome annotation databases

GeneIDi24931.
KEGGirno:24931.
UCSCiRGD:2317. rat.

Similar proteinsi

Entry informationi

Entry nameiCD8B_RAT
AccessioniPrimary (citable) accession number: P05541
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: November 1, 1988
Last modified: November 22, 2017
This is version 130 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome