Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

T-cell surface glycoprotein CD4

Gene

Cd4

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processAdaptive immunity, Immunity

Enzyme and pathway databases

ReactomeiR-RNO-1462054 Alpha-defensins
R-RNO-202424 Downstream TCR signaling
R-RNO-202427 Phosphorylation of CD3 and TCR zeta chains
R-RNO-202430 Translocation of ZAP-70 to Immunological synapse
R-RNO-202433 Generation of second messenger molecules
R-RNO-389948 PD-1 signaling
R-RNO-449836 Other interleukin signaling
R-RNO-8856825 Cargo recognition for clathrin-mediated endocytosis
R-RNO-8856828 Clathrin-mediated endocytosis

Names & Taxonomyi

Protein namesi
Recommended name:
T-cell surface glycoprotein CD4
Alternative name(s):
T-cell surface antigen T4/Leu-3
W3/25 antigen
CD_antigen: CD4
Gene namesi
Name:Cd4
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 4

Organism-specific databases

RGDi2306 Cd4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini28 – 394ExtracellularSequence analysisAdd BLAST367
Transmembranei395 – 417HelicalSequence analysisAdd BLAST23
Topological domaini418 – 457CytoplasmicSequence analysisAdd BLAST40

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi186N → T: No change in secretion; when associated with S-297. 1 Publication1
Mutagenesisi297N → S: No change in secretion; when associated with T-186. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 27Add BLAST27
ChainiPRO_000001462928 – 457T-cell surface glycoprotein CD4Add BLAST430

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi43 ↔ 111PROSITE-ProRule annotation1 Publication
Disulfide bondi158 ↔ 187PROSITE-ProRule annotation1 Publication
Glycosylationi186N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi297N-linked (GlcNAc...) asparagine1 Publication1
Disulfide bondi328 ↔ 370PROSITE-ProRule annotation1 Publication
Glycosylationi392N-linked (GlcNAc...) asparagineSequence analysis1
Lipidationi418S-palmitoyl cysteineBy similarity1
Lipidationi421S-palmitoyl cysteineBy similarity1
Modified residuei432PhosphoserineBy similarity1
Modified residuei439PhosphoserineBy similarity1

Post-translational modificationi

Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.By similarity
Phosphorylated by PKC; phosphorylation plays an important role for CD4 internalization.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiP05540
PRIDEiP05540

PTM databases

GlyConnecti588
iPTMnetiP05540
UniCarbKBiP05540

Expressioni

Gene expression databases

BgeeiENSRNOG00000016294
GenevisibleiP05540 RN

Interactioni

Subunit structurei

Forms disulfide-linked homo-dimers at the cell surface. Interacts with LCK. Interacts with PTK2/FAK1. Binds to P4HB/PDI. Interacts with IL16; this interaction induces a CD4-dependent signaling in lymphocytes.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000021915

Structurei

Secondary structure

1457
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi213 – 217Combined sources5
Beta strandi222 – 225Combined sources4
Beta strandi235 – 245Combined sources11
Beta strandi252 – 258Combined sources7
Beta strandi261 – 266Combined sources6
Beta strandi282 – 285Combined sources4
Helixi290 – 292Combined sources3
Beta strandi294 – 301Combined sources8
Beta strandi303 – 319Combined sources17
Beta strandi321 – 332Combined sources12
Beta strandi336 – 344Combined sources9
Beta strandi349 – 362Combined sources14
Beta strandi365 – 374Combined sources10
Beta strandi377 – 385Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CIDX-ray2.80A210-386[»]
ProteinModelPortaliP05540
SMRiP05540
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP05540

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini28 – 127Ig-like V-typeAdd BLAST100
Domaini128 – 206Ig-like C2-type 1Add BLAST79
Domaini207 – 316Ig-like C2-type 2Add BLAST110
Domaini317 – 374Ig-like C2-type 3Add BLAST58

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IK9F Eukaryota
ENOG410YWI4 LUCA
GeneTreeiENSGT00390000001745
HOGENOMiHOG000008696
HOVERGENiHBG005281
InParanoidiP05540
KOiK06454
OMAiPEAGMWQ
OrthoDBiEOG091G0AZL
PhylomeDBiP05540
TreeFamiTF335974

Family and domain databases

Gene3Di2.60.40.10, 4 hits
InterProiView protein in InterPro
IPR000973 CD4
IPR015274 CD4-extracel
IPR007110 Ig-like_dom
IPR036179 Ig-like_dom_sf
IPR013783 Ig-like_fold
IPR008424 Ig_C2-set
IPR003599 Ig_sub
IPR013106 Ig_V-set
IPR013151 Immunoglobulin
IPR021963 Tcell_CD4_Cterm
PANTHERiPTHR11422:SF0 PTHR11422:SF0, 1 hit
PfamiView protein in Pfam
PF05790 C2-set, 2 hits
PF09191 CD4-extracel, 1 hit
PF00047 ig, 1 hit
PF12104 Tcell_CD4_C, 1 hit
PRINTSiPR00692 CD4TCANTIGEN
SMARTiView protein in SMART
SM00409 IG, 3 hits
SM00406 IGv, 1 hit
SUPFAMiSSF48726 SSF48726, 3 hits
PROSITEiView protein in PROSITE
PS50835 IG_LIKE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P05540-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCRGFSFRHL LPLLLLQLSK LLVVTQGKTV VLGKEGGSAE LPCESTSRRS
60 70 80 90 100
ASFAWKSSDQ KTILGYKNKL LIKGSLELYS RFDSRKNAWE RGSFPLIINK
110 120 130 140 150
LRMEDSQTYV CELENKKEEV ELWVFRVTFN PGTRLLQGQS LTLILDSNPK
160 170 180 190 200
VSDPPIECKH KSSNIVKDSK AFSTHSLRIQ DSGIWNCTVT LNQKKHSFDM
210 220 230 240 250
KLSVLGFAST SITAYKSEGE SAEFSFPLNL GEESLQGELR WKAEKAPSSQ
260 270 280 290 300
SWITFSLKNQ KVSVQKSTSN PKFQLSETLP LTLQIPQVSL QFAGSGNLTL
310 320 330 340 350
TLDRGILYQE VNLVVMKVTQ PDSNTLTCEV MGPTSPKMRL ILKQENQEAR
360 370 380 390 400
VSRQEKVIQV QAPEAGVWQC LLSEGEEVKM DSKIQVLSKG LNQTMFLAVV
410 420 430 440 450
LGSAFSFLVF TGLCILFCVR CRHQQRQAAR MSQIKRLLSE KKTCQCSHRM

QKSHNLI
Length:457
Mass (Da):51,438
Last modified:November 1, 1988 - v1
Checksum:i477BE157D30954C1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15768 mRNA Translation: AAA40901.1
PIRiA27449
RefSeqiNP_036837.1, NM_012705.1
XP_006237393.1, XM_006237331.3
XP_008761502.1, XM_008763280.2
UniGeneiRn.10748

Genome annotation databases

EnsembliENSRNOT00000021915; ENSRNOP00000021915; ENSRNOG00000016294
GeneIDi24932
KEGGirno:24932
UCSCiRGD:2306 rat

Similar proteinsi

Entry informationi

Entry nameiCD4_RAT
AccessioniPrimary (citable) accession number: P05540
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: November 1, 1988
Last modified: May 23, 2018
This is version 164 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health