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P05540

- CD4_RAT

UniProt

P05540 - CD4_RAT

Protein

T-cell surface glycoprotein CD4

Gene

Cd4

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 135 (01 Oct 2014)
      Sequence version 1 (01 Nov 1988)
      Previous versions | rss
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    Functioni

    Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation.

    GO - Molecular functioni

    1. protein kinase binding Source: RGD
    2. zinc ion binding Source: Ensembl

    GO - Biological processi

    1. cell adhesion Source: InterPro
    2. cell surface receptor signaling pathway Source: Ensembl
    3. cytokine production Source: Ensembl
    4. defense response to Gram-negative bacterium Source: Ensembl
    5. immune response Source: InterPro
    6. induction by virus of host cell-cell fusion Source: Ensembl
    7. maintenance of protein location in cell Source: Ensembl
    8. positive regulation of calcium-mediated signaling Source: Ensembl
    9. positive regulation of peptidyl-tyrosine phosphorylation Source: Ensembl
    10. positive regulation of protein kinase activity Source: Ensembl
    11. positive regulation of T cell activation Source: Ensembl
    12. protein palmitoleylation Source: Ensembl
    13. T cell activation Source: RGD
    14. T cell differentiation Source: UniProtKB
    15. T cell selection Source: UniProtKB

    Keywords - Biological processi

    Adaptive immunity, Immunity

    Enzyme and pathway databases

    ReactomeiREACT_199179. Alpha-defensins.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    T-cell surface glycoprotein CD4
    Alternative name(s):
    T-cell surface antigen T4/Leu-3
    W3/25 antigen
    CD_antigen: CD4
    Gene namesi
    Name:Cd4
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 4

    Organism-specific databases

    RGDi2306. Cd4.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum lumen Source: Ensembl
    2. endoplasmic reticulum membrane Source: Ensembl
    3. external side of plasma membrane Source: Ensembl
    4. integral component of membrane Source: UniProtKB-KW
    5. membrane raft Source: Ensembl

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi186 – 1861N → T: No change in secretion; when associated with S-297. 1 Publication
    Mutagenesisi297 – 2971N → S: No change in secretion; when associated with T-186. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2727Add
    BLAST
    Chaini28 – 457430T-cell surface glycoprotein CD4PRO_0000014629Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi43 ↔ 1111 PublicationPROSITE-ProRule annotation
    Disulfide bondi158 ↔ 1871 PublicationPROSITE-ProRule annotation
    Glycosylationi186 – 1861N-linked (GlcNAc...)1 Publication
    Glycosylationi297 – 2971N-linked (GlcNAc...)1 Publication
    Disulfide bondi328 ↔ 3701 PublicationPROSITE-ProRule annotation
    Glycosylationi392 – 3921N-linked (GlcNAc...)Sequence Analysis
    Lipidationi418 – 4181S-palmitoyl cysteineBy similarity
    Lipidationi421 – 4211S-palmitoyl cysteineBy similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

    Proteomic databases

    PRIDEiP05540.

    PTM databases

    UniCarbKBiP05540.

    Expressioni

    Gene expression databases

    GenevestigatoriP05540.

    Interactioni

    Subunit structurei

    Associates with p56-LCK. Interacts with SPG21 By similarity.By similarity

    Protein-protein interaction databases

    BioGridi247037. 1 interaction.
    MINTiMINT-4996350.
    STRINGi10116.ENSRNOP00000021915.

    Structurei

    Secondary structure

    1
    457
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi213 – 2175
    Beta strandi222 – 2254
    Beta strandi235 – 24511
    Beta strandi252 – 2587
    Beta strandi261 – 2666
    Beta strandi282 – 2854
    Helixi290 – 2923
    Beta strandi294 – 3018
    Beta strandi303 – 31917
    Beta strandi321 – 33212
    Beta strandi336 – 3449
    Beta strandi349 – 36214
    Beta strandi365 – 37410
    Beta strandi377 – 3859

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1CIDX-ray2.80A210-386[»]
    ProteinModelPortaliP05540.
    SMRiP05540. Positions 210-386, 420-453.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP05540.

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini28 – 394367ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini418 – 45740CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei395 – 41723HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini28 – 127100Ig-like V-typeAdd
    BLAST
    Domaini128 – 20679Ig-like C2-type 1Add
    BLAST
    Domaini207 – 316110Ig-like C2-type 2Add
    BLAST
    Domaini317 – 37458Ig-like C2-type 3Add
    BLAST

    Sequence similaritiesi

    Keywords - Domaini

    Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG47205.
    GeneTreeiENSGT00390000001745.
    HOGENOMiHOG000008696.
    HOVERGENiHBG005281.
    InParanoidiP05540.
    KOiK06454.
    OMAiPEAGMWQ.
    OrthoDBiEOG7HXCQS.
    PhylomeDBiP05540.
    TreeFamiTF335974.

    Family and domain databases

    Gene3Di2.60.40.10. 4 hits.
    InterProiIPR000973. Ag_CD4.
    IPR015274. CD4-extracel.
    IPR007110. Ig-like_dom.
    IPR013783. Ig-like_fold.
    IPR008424. Ig_C2-set.
    IPR003599. Ig_sub.
    IPR013106. Ig_V-set.
    IPR021963. Tcell_CD4_Cterm.
    [Graphical view]
    PfamiPF05790. C2-set. 2 hits.
    PF09191. CD4-extracel. 1 hit.
    PF12104. Tcell_CD4_Cterm. 1 hit.
    PF07686. V-set. 1 hit.
    [Graphical view]
    PRINTSiPR00692. CD4TCANTIGEN.
    SMARTiSM00409. IG. 2 hits.
    [Graphical view]
    PROSITEiPS50835. IG_LIKE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P05540-1 [UniParc]FASTAAdd to Basket

    « Hide

    MCRGFSFRHL LPLLLLQLSK LLVVTQGKTV VLGKEGGSAE LPCESTSRRS    50
    ASFAWKSSDQ KTILGYKNKL LIKGSLELYS RFDSRKNAWE RGSFPLIINK 100
    LRMEDSQTYV CELENKKEEV ELWVFRVTFN PGTRLLQGQS LTLILDSNPK 150
    VSDPPIECKH KSSNIVKDSK AFSTHSLRIQ DSGIWNCTVT LNQKKHSFDM 200
    KLSVLGFAST SITAYKSEGE SAEFSFPLNL GEESLQGELR WKAEKAPSSQ 250
    SWITFSLKNQ KVSVQKSTSN PKFQLSETLP LTLQIPQVSL QFAGSGNLTL 300
    TLDRGILYQE VNLVVMKVTQ PDSNTLTCEV MGPTSPKMRL ILKQENQEAR 350
    VSRQEKVIQV QAPEAGVWQC LLSEGEEVKM DSKIQVLSKG LNQTMFLAVV 400
    LGSAFSFLVF TGLCILFCVR CRHQQRQAAR MSQIKRLLSE KKTCQCSHRM 450
    QKSHNLI 457
    Length:457
    Mass (Da):51,438
    Last modified:November 1, 1988 - v1
    Checksum:i477BE157D30954C1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M15768 mRNA. Translation: AAA40901.1.
    PIRiA27449.
    RefSeqiNP_036837.1. NM_012705.1.
    XP_006237393.1. XM_006237331.1.
    UniGeneiRn.10748.

    Genome annotation databases

    EnsembliENSRNOT00000021915; ENSRNOP00000021915; ENSRNOG00000016294.
    GeneIDi24932.
    KEGGirno:24932.
    UCSCiRGD:2306. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M15768 mRNA. Translation: AAA40901.1 .
    PIRi A27449.
    RefSeqi NP_036837.1. NM_012705.1.
    XP_006237393.1. XM_006237331.1.
    UniGenei Rn.10748.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1CID X-ray 2.80 A 210-386 [» ]
    ProteinModelPortali P05540.
    SMRi P05540. Positions 210-386, 420-453.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 247037. 1 interaction.
    MINTi MINT-4996350.
    STRINGi 10116.ENSRNOP00000021915.

    PTM databases

    UniCarbKBi P05540.

    Proteomic databases

    PRIDEi P05540.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000021915 ; ENSRNOP00000021915 ; ENSRNOG00000016294 .
    GeneIDi 24932.
    KEGGi rno:24932.
    UCSCi RGD:2306. rat.

    Organism-specific databases

    CTDi 920.
    RGDi 2306. Cd4.

    Phylogenomic databases

    eggNOGi NOG47205.
    GeneTreei ENSGT00390000001745.
    HOGENOMi HOG000008696.
    HOVERGENi HBG005281.
    InParanoidi P05540.
    KOi K06454.
    OMAi PEAGMWQ.
    OrthoDBi EOG7HXCQS.
    PhylomeDBi P05540.
    TreeFami TF335974.

    Enzyme and pathway databases

    Reactomei REACT_199179. Alpha-defensins.

    Miscellaneous databases

    EvolutionaryTracei P05540.
    NextBioi 604903.
    PROi P05540.

    Gene expression databases

    Genevestigatori P05540.

    Family and domain databases

    Gene3Di 2.60.40.10. 4 hits.
    InterProi IPR000973. Ag_CD4.
    IPR015274. CD4-extracel.
    IPR007110. Ig-like_dom.
    IPR013783. Ig-like_fold.
    IPR008424. Ig_C2-set.
    IPR003599. Ig_sub.
    IPR013106. Ig_V-set.
    IPR021963. Tcell_CD4_Cterm.
    [Graphical view ]
    Pfami PF05790. C2-set. 2 hits.
    PF09191. CD4-extracel. 1 hit.
    PF12104. Tcell_CD4_Cterm. 1 hit.
    PF07686. V-set. 1 hit.
    [Graphical view ]
    PRINTSi PR00692. CD4TCANTIGEN.
    SMARTi SM00409. IG. 2 hits.
    [Graphical view ]
    PROSITEi PS50835. IG_LIKE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Peptide and nucleotide sequences of rat CD4 (W3/25) antigen: evidence for derivation from a structure with four immunoglobulin-related domains."
      Clark S.J., Jefferies W.A., Barclay A.N., Gagnon J., Williams A.F.
      Proc. Natl. Acad. Sci. U.S.A. 84:1649-1653(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    2. "High level expression in Chinese hamster ovary cells of soluble forms of CD4 T lymphocyte glycoprotein including glycosylation variants."
      Davis S.J., Ward H.A., Puklavec M.J., Willis A.C., Williams A.F., Barclay A.N.
      J. Biol. Chem. 265:10410-10418(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 42-51; 83-88; 95-101; 109-112; 146-164; 178-192; 256-273; 292-300; 327-329 AND 368-371, DISULFIDE BONDS, GLYCOSYLATION AT ASN-186 AND ASN-297, MUTAGENESIS OF ASN-186 AND ASN-297.
    3. "Crystal structure of domains 3 and 4 of rat CD4: relation to the NH2-terminal domains."
      Brady R.L., Dodson E.J., Dodson G.G., Lange G., Davis S.J., Williams A.F., Barclay A.N.
      Science 260:979-983(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 210-393.

    Entry informationi

    Entry nameiCD4_RAT
    AccessioniPrimary (citable) accession number: P05540
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1988
    Last sequence update: November 1, 1988
    Last modified: October 1, 2014
    This is version 135 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3