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P05540

- CD4_RAT

UniProt

P05540 - CD4_RAT

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Protein

T-cell surface glycoprotein CD4

Gene

Cd4

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation.

GO - Molecular functioni

  1. coreceptor activity Source: InterPro
  2. protein kinase binding Source: RGD
  3. zinc ion binding Source: Ensembl

GO - Biological processi

  1. cell adhesion Source: InterPro
  2. cell surface receptor signaling pathway Source: Ensembl
  3. cytokine production Source: Ensembl
  4. defense response to Gram-negative bacterium Source: Ensembl
  5. immune response Source: InterPro
  6. induction by virus of host cell-cell fusion Source: Ensembl
  7. maintenance of protein location in cell Source: Ensembl
  8. positive regulation of calcium-mediated signaling Source: Ensembl
  9. positive regulation of peptidyl-tyrosine phosphorylation Source: Ensembl
  10. positive regulation of protein kinase activity Source: Ensembl
  11. positive regulation of T cell activation Source: Ensembl
  12. protein palmitoleylation Source: Ensembl
  13. T cell activation Source: RGD
  14. T cell differentiation Source: UniProtKB
  15. T cell selection Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Adaptive immunity, Immunity

Enzyme and pathway databases

ReactomeiREACT_199179. Alpha-defensins.

Names & Taxonomyi

Protein namesi
Recommended name:
T-cell surface glycoprotein CD4
Alternative name(s):
T-cell surface antigen T4/Leu-3
W3/25 antigen
CD_antigen: CD4
Gene namesi
Name:Cd4
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 4

Organism-specific databases

RGDi2306. Cd4.

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum lumen Source: Ensembl
  2. endoplasmic reticulum membrane Source: Ensembl
  3. external side of plasma membrane Source: Ensembl
  4. integral component of membrane Source: UniProtKB-KW
  5. membrane raft Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi186 – 1861N → T: No change in secretion; when associated with S-297. 1 Publication
Mutagenesisi297 – 2971N → S: No change in secretion; when associated with T-186. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727Add
BLAST
Chaini28 – 457430T-cell surface glycoprotein CD4PRO_0000014629Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi43 ↔ 1111 PublicationPROSITE-ProRule annotation
Disulfide bondi158 ↔ 1871 PublicationPROSITE-ProRule annotation
Glycosylationi186 – 1861N-linked (GlcNAc...)1 Publication
Glycosylationi297 – 2971N-linked (GlcNAc...)1 Publication
Disulfide bondi328 ↔ 3701 PublicationPROSITE-ProRule annotation
Glycosylationi392 – 3921N-linked (GlcNAc...)Sequence Analysis
Lipidationi418 – 4181S-palmitoyl cysteineBy similarity
Lipidationi421 – 4211S-palmitoyl cysteineBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

Proteomic databases

PRIDEiP05540.

PTM databases

UniCarbKBiP05540.

Expressioni

Gene expression databases

GenevestigatoriP05540.

Interactioni

Subunit structurei

Associates with p56-LCK. Interacts with SPG21 (By similarity).By similarity

Protein-protein interaction databases

BioGridi247037. 1 interaction.
MINTiMINT-4996350.
STRINGi10116.ENSRNOP00000021915.

Structurei

Secondary structure

1
457
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi213 – 2175
Beta strandi222 – 2254
Beta strandi235 – 24511
Beta strandi252 – 2587
Beta strandi261 – 2666
Beta strandi282 – 2854
Helixi290 – 2923
Beta strandi294 – 3018
Beta strandi303 – 31917
Beta strandi321 – 33212
Beta strandi336 – 3449
Beta strandi349 – 36214
Beta strandi365 – 37410
Beta strandi377 – 3859

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1CIDX-ray2.80A210-386[»]
ProteinModelPortaliP05540.
SMRiP05540. Positions 210-386, 420-453.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP05540.

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini28 – 394367ExtracellularSequence AnalysisAdd
BLAST
Topological domaini418 – 45740CytoplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei395 – 41723HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini28 – 127100Ig-like V-typeAdd
BLAST
Domaini128 – 20679Ig-like C2-type 1Add
BLAST
Domaini207 – 316110Ig-like C2-type 2Add
BLAST
Domaini317 – 37458Ig-like C2-type 3Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG47205.
GeneTreeiENSGT00390000001745.
HOGENOMiHOG000008696.
HOVERGENiHBG005281.
InParanoidiP05540.
KOiK06454.
OMAiPEAGMWQ.
OrthoDBiEOG7HXCQS.
PhylomeDBiP05540.
TreeFamiTF335974.

Family and domain databases

Gene3Di2.60.40.10. 4 hits.
InterProiIPR000973. CD4.
IPR015274. CD4-extracel.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR008424. Ig_C2-set.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
IPR021963. Tcell_CD4_Cterm.
[Graphical view]
PfamiPF05790. C2-set. 2 hits.
PF09191. CD4-extracel. 1 hit.
PF12104. Tcell_CD4_Cterm. 1 hit.
PF07686. V-set. 1 hit.
[Graphical view]
PRINTSiPR00692. CD4TCANTIGEN.
SMARTiSM00409. IG. 2 hits.
[Graphical view]
PROSITEiPS50835. IG_LIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P05540-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MCRGFSFRHL LPLLLLQLSK LLVVTQGKTV VLGKEGGSAE LPCESTSRRS
60 70 80 90 100
ASFAWKSSDQ KTILGYKNKL LIKGSLELYS RFDSRKNAWE RGSFPLIINK
110 120 130 140 150
LRMEDSQTYV CELENKKEEV ELWVFRVTFN PGTRLLQGQS LTLILDSNPK
160 170 180 190 200
VSDPPIECKH KSSNIVKDSK AFSTHSLRIQ DSGIWNCTVT LNQKKHSFDM
210 220 230 240 250
KLSVLGFAST SITAYKSEGE SAEFSFPLNL GEESLQGELR WKAEKAPSSQ
260 270 280 290 300
SWITFSLKNQ KVSVQKSTSN PKFQLSETLP LTLQIPQVSL QFAGSGNLTL
310 320 330 340 350
TLDRGILYQE VNLVVMKVTQ PDSNTLTCEV MGPTSPKMRL ILKQENQEAR
360 370 380 390 400
VSRQEKVIQV QAPEAGVWQC LLSEGEEVKM DSKIQVLSKG LNQTMFLAVV
410 420 430 440 450
LGSAFSFLVF TGLCILFCVR CRHQQRQAAR MSQIKRLLSE KKTCQCSHRM

QKSHNLI
Length:457
Mass (Da):51,438
Last modified:November 1, 1988 - v1
Checksum:i477BE157D30954C1
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M15768 mRNA. Translation: AAA40901.1.
PIRiA27449.
RefSeqiNP_036837.1. NM_012705.1.
XP_006237393.1. XM_006237331.2.
UniGeneiRn.10748.

Genome annotation databases

EnsembliENSRNOT00000021915; ENSRNOP00000021915; ENSRNOG00000016294.
GeneIDi24932.
KEGGirno:24932.
UCSCiRGD:2306. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M15768 mRNA. Translation: AAA40901.1 .
PIRi A27449.
RefSeqi NP_036837.1. NM_012705.1.
XP_006237393.1. XM_006237331.2.
UniGenei Rn.10748.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1CID X-ray 2.80 A 210-386 [» ]
ProteinModelPortali P05540.
SMRi P05540. Positions 210-386, 420-453.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 247037. 1 interaction.
MINTi MINT-4996350.
STRINGi 10116.ENSRNOP00000021915.

PTM databases

UniCarbKBi P05540.

Proteomic databases

PRIDEi P05540.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000021915 ; ENSRNOP00000021915 ; ENSRNOG00000016294 .
GeneIDi 24932.
KEGGi rno:24932.
UCSCi RGD:2306. rat.

Organism-specific databases

CTDi 920.
RGDi 2306. Cd4.

Phylogenomic databases

eggNOGi NOG47205.
GeneTreei ENSGT00390000001745.
HOGENOMi HOG000008696.
HOVERGENi HBG005281.
InParanoidi P05540.
KOi K06454.
OMAi PEAGMWQ.
OrthoDBi EOG7HXCQS.
PhylomeDBi P05540.
TreeFami TF335974.

Enzyme and pathway databases

Reactomei REACT_199179. Alpha-defensins.

Miscellaneous databases

EvolutionaryTracei P05540.
NextBioi 604903.
PROi P05540.

Gene expression databases

Genevestigatori P05540.

Family and domain databases

Gene3Di 2.60.40.10. 4 hits.
InterProi IPR000973. CD4.
IPR015274. CD4-extracel.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR008424. Ig_C2-set.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
IPR021963. Tcell_CD4_Cterm.
[Graphical view ]
Pfami PF05790. C2-set. 2 hits.
PF09191. CD4-extracel. 1 hit.
PF12104. Tcell_CD4_Cterm. 1 hit.
PF07686. V-set. 1 hit.
[Graphical view ]
PRINTSi PR00692. CD4TCANTIGEN.
SMARTi SM00409. IG. 2 hits.
[Graphical view ]
PROSITEi PS50835. IG_LIKE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Peptide and nucleotide sequences of rat CD4 (W3/25) antigen: evidence for derivation from a structure with four immunoglobulin-related domains."
    Clark S.J., Jefferies W.A., Barclay A.N., Gagnon J., Williams A.F.
    Proc. Natl. Acad. Sci. U.S.A. 84:1649-1653(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
  2. "High level expression in Chinese hamster ovary cells of soluble forms of CD4 T lymphocyte glycoprotein including glycosylation variants."
    Davis S.J., Ward H.A., Puklavec M.J., Willis A.C., Williams A.F., Barclay A.N.
    J. Biol. Chem. 265:10410-10418(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 42-51; 83-88; 95-101; 109-112; 146-164; 178-192; 256-273; 292-300; 327-329 AND 368-371, DISULFIDE BONDS, GLYCOSYLATION AT ASN-186 AND ASN-297, MUTAGENESIS OF ASN-186 AND ASN-297.
  3. "Crystal structure of domains 3 and 4 of rat CD4: relation to the NH2-terminal domains."
    Brady R.L., Dodson E.J., Dodson G.G., Lange G., Davis S.J., Williams A.F., Barclay A.N.
    Science 260:979-983(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 210-393.

Entry informationi

Entry nameiCD4_RAT
AccessioniPrimary (citable) accession number: P05540
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: November 1, 1988
Last modified: October 29, 2014
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3