Reviewed, UniProtKB/Swiss-Prot P05540 (CD4_RAT)

Names and origin

Protein names

Recommended name:
    T-cell surface glycoprotein CD4
Alternative name(s):
    T-cell surface antigen T4/Leu-3
    W3/25 antigen
    CD_antigen=CD4

Gene names

Name:

Cd4

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	457 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation.
Subunit structure	Associates with p56-LCK. Interacts with SPG21 By similarity.
Subcellular location	Membrane; Single-pass type I membrane protein.
Sequence similarities	Contains 3 Ig-like C2-type (immunoglobulin-like) domains. Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Ontologies

Keywords
Biological process	Immune response
Cellular component	Membrane
Domain	Immunoglobulin domain Repeat Signal Transmembrane
PTM	Disulfide bond Glycoprotein Lipoprotein Palmitate
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	T cell selection Inferred from sequence or structural similarity. Source: UniProtKB cell adhesion Inferred from electronic annotation. Source: InterPro immune response Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 27

27

Chain

28 – 457

430

T-cell surface glycoprotein CD4

PRO_0000014629

Regions

Topological domain

28 – 394

367

Extracellular Potential

Transmembrane

395 – 417

23

Potential

Topological domain

418 – 457

40

Cytoplasmic Potential

Domain

28 – 127

100

Ig-like V-type

Domain

128 – 206

79

Ig-like C2-type 1

Domain

207 – 316

110

Ig-like C2-type 2

Domain

317 – 374

58

Ig-like C2-type 3

Amino acid modifications

Lipidation

418

1

S-palmitoyl cysteine By similarity

Lipidation

421

1

S-palmitoyl cysteine By similarity

Glycosylation

186

1

N-linked (GlcNAc...) Ref.2

Glycosylation

297

1

N-linked (GlcNAc...) Ref.2

Glycosylation

392

1

N-linked (GlcNAc...) Potential

Disulfide bond

43 ↔ 111

Ref.2

Disulfide bond

158 ↔ 187

Ref.2

Disulfide bond

328 ↔ 370

Ref.2

Experimental info

Mutagenesis

186

1

N → T: No change in secretion; when associated with S-297. Ref.2

Mutagenesis

297

1

N → S: No change in secretion; when associated with T-186. Ref.2

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

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.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

457

Helix Strand Turn

Details...

Beta strand

213 – 217

5

Beta strand

222 – 225

4

Beta strand

235 – 245

11

Beta strand

252 – 258

7

Beta strand

261 – 266

6

Beta strand

282 – 285

4

Helix

290 – 292

3

Beta strand

294 – 301

8

Beta strand

303 – 319

17

Beta strand

321 – 332

12

Beta strand

336 – 344

9

Beta strand

349 – 362

14

Beta strand

365 – 374

10

Beta strand

377 – 385

9

Sequences

	Sequence		Length	Mass (Da)
	P05540-1 [UniParc]. Last modified November 1, 1988. Version 1. Checksum: 477BE157D30954C1	FASTA	457	51,438
	10 20 30 40 50 60 MCRGFSFRHL LPLLLLQLSK LLVVTQGKTV VLGKEGGSAE LPCESTSRRS ASFAWKSSDQ 70 80 90 100 110 120 KTILGYKNKL LIKGSLELYS RFDSRKNAWE RGSFPLIINK LRMEDSQTYV CELENKKEEV 130 140 150 160 170 180 ELWVFRVTFN PGTRLLQGQS LTLILDSNPK VSDPPIECKH KSSNIVKDSK AFSTHSLRIQ 190 200 210 220 230 240 DSGIWNCTVT LNQKKHSFDM KLSVLGFAST SITAYKSEGE SAEFSFPLNL GEESLQGELR 250 260 270 280 290 300 WKAEKAPSSQ SWITFSLKNQ KVSVQKSTSN PKFQLSETLP LTLQIPQVSL QFAGSGNLTL 310 320 330 340 350 360 TLDRGILYQE VNLVVMKVTQ PDSNTLTCEV MGPTSPKMRL ILKQENQEAR VSRQEKVIQV 370 380 390 400 410 420 QAPEAGVWQC LLSEGEEVKM DSKIQVLSKG LNQTMFLAVV LGSAFSFLVF TGLCILFCVR 430 440 450 CRHQQRQAAR MSQIKRLLSE KKTCQCSHRM QKSHNLI

References

[1]	"Peptide and nucleotide sequences of rat CD4 (W3/25) antigen: evidence for derivation from a structure with four immunoglobulin-related domains." Clark S.J., Jefferies W.A., Barclay A.N., Gagnon J., Williams A.F. Proc. Natl. Acad. Sci. U.S.A. 84:1649-1653(1987) [PubMed: 3104900] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
[2]	"High level expression in Chinese hamster ovary cells of soluble forms of CD4 T lymphocyte glycoprotein including glycosylation variants." Davis S.J., Ward H.A., Puklavec M.J., Willis A.C., Williams A.F., Barclay A.N. J. Biol. Chem. 265:10410-10418(1990) [PubMed: 2113054] [Abstract] Cited for: PROTEIN SEQUENCE OF 42-51; 83-88; 95-101; 109-112; 146-164; 178-192; 256-273; 292-300; 327-329 AND 368-371, DISULFIDE BONDS, GLYCOSYLATION AT ASN-186 AND ASN-297, MUTAGENESIS OF ASN-186 AND ASN-297.
[3]	"Crystal structure of domains 3 and 4 of rat CD4: relation to the NH2-terminal domains." Brady R.L., Dodson E.J., Dodson G.G., Lange G., Davis S.J., Williams A.F., Barclay A.N. Science 260:979-983(1993) [PubMed: 8493535] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 210-393.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

M15768 mRNA. Translation: AAA40901.1.

IPI

IPI00200583.

PIR

A27449.

RefSeq

NP_036837.1.

UniGene

Rn.10748

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1CID	X-ray	2.80	A	210-386	[»]

ModBase

Search...

Protein-protein interaction databases

STRING

P05540.

PTM databases

GlycoSuiteDB

P05540.

Proteomic databases

PRIDE

P05540.

Genome annotation databases

Ensembl

ENSRNOT00000021915; ENSRNOP00000021915; ENSRNOG00000016294; Rattus norvegicus. [Genome view]

GeneID

24932.

KEGG

rno:24932.

UCSC

NM_012705. rat.

Organism-specific databases

CTD

24932.

RGD

2306. Cd4.

Phylogenomic databases

HOVERGEN

P05540.

OMA

QAERMSQ.

Gene expression databases

ArrayExpress

P05540.

Genevestigator

P05540.

GermOnline

ENSRNOG00000016294. Rattus norvegicus.

Family and domain databases

InterPro

IPR000973. Ag_CD4.
IPR015274. CD4-extracel.
IPR013151. Ig.
IPR007110. Ig-like.
IPR013783. Ig-like_fold.
IPR008424. Ig_C2-set.
IPR003599. Ig_sub.
[Graphical view]

Gene3D

G3DSA:2.60.40.10. Ig-like_fold. 4 hits.

Pfam

PF05790. C2-set. 2 hits.
PF09191. CD4-extracel. 1 hit.
PF00047. ig. 1 hit.
[Graphical view]

PRINTS

PR00692. CD4TCANTIGEN.

SMART

SM00409. IG. 2 hits.
[Graphical view]

PROSITE

PS50835. IG_LIKE. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

604903.

Entry information

Entry name

CD4_RAT

Accession

Primary (citable) accession number: P05540

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1988
Last sequence update:	November 1, 1988
Last modified:	November 3, 2009
This is version 99 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)