P05534 (1A24_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
HLA class I histocompatibility antigen, A-24 alpha chain

Alternative name(s):
Aw-24
HLA class I histocompatibility antigen, A-9 alpha chain
MHC class I antigen A*24

Gene names

Name:	HLA-A
Synonyms:	HLAA

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	365 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the presentation of foreign antigens to the immune system.
Subunit structure	Heterodimer of an alpha chain and a beta chain (beta-2-microglobulin). Interacts with human herpesvirus 8 MIR1 protein By similarity.
Subcellular location	Membrane; Single-pass type I membrane protein.
Post-translational modification	Polyubiquitinated in a post ER compartment by interaction with human herpesvirus 8 MIR1 protein. This targets the protein for rapid degradation via the ubiquitin system By similarity.
Polymorphism	The following alleles of A-24 are known: A*2401, A*24:02, A*24:03, A*24:06, A*24:08 (A9HH), A*24:10 (A*24JV), A*24:13 (A*24YM), A*24:14 (A*24SA) and A*24:29. Allele A*24:02 is represented in all major racial groups. Allele A*24:06 and allele A*24:13 are found in the Australian Aboriginal population. Allele A*24:14 is found in individuals of South American descent. The sequence shown is that of A*24:02.
Sequence similarities	Belongs to the MHC class I family. Contains 1 Ig-like C1-type (immunoglobulin-like) domain.

Ontologies

Keywords
Biological process	Host-virus interaction Immunity
Cellular component	MHC I Membrane
Coding sequence diversity	Polymorphism
Domain	Signal Transmembrane Transmembrane helix
PTM	Disulfide bond Glycoprotein Phosphoprotein Ubl conjugation
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent Traceable author statement. Source: Reactome antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent Traceable author statement. Source: Reactome interferon-gamma-mediated signaling pathway Traceable author statement. Source: Reactome modulation by virus of host morphology or physiology Inferred from electronic annotation. Source: UniProtKB-KW positive regulation of T cell mediated cytotoxicity Inferred from electronic annotation. Source: InterPro regulation of immune response Traceable author statement. Source: Reactome type I interferon-mediated signaling pathway Traceable author statement. Source: Reactome
Cellular_component	ER to Golgi transport vesicle membrane Traceable author statement. Source: Reactome Golgi membrane Traceable author statement. Source: Reactome MHC class I protein complex Inferred from electronic annotation. Source: UniProtKB-KW early endosome membrane Traceable author statement. Source: Reactome integral to lumenal side of endoplasmic reticulum membrane Traceable author statement. Source: Reactome phagocytic vesicle membrane Traceable author statement. Source: Reactome plasma membrane Traceable author statement. Source: Reactome
Molecular_function	peptide antigen binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 24

24

Chain

25 – 365

341

HLA class I histocompatibility antigen, A-24 alpha chain

PRO_0000018818

Regions

Topological domain

25 – 308

284

Extracellular Potential

Transmembrane

309 – 332

24

Helical; Potential

Topological domain

333 – 365

33

Cytoplasmic Potential

Domain

209 – 295

87

Ig-like C1-type

Region

25 – 114

90

Alpha-1

Region

115 – 206

92

Alpha-2

Region

207 – 298

92

Alpha-3

Region

299 – 308

10

Connecting peptide

Amino acid modifications

Modified residue

356

1

Phosphoserine Ref.10

Glycosylation

110

1

N-linked (GlcNAc...) Ref.12

Disulfide bond

125 ↔ 188

Ref.13

Disulfide bond

227 ↔ 283

Ref.13

Natural variations

Natural variant

5

1

A → G in allele A*2401.

VAR_004354

Natural variant

27

1

H → Q in allele A*24:08.
Corresponds to variant rs41541319 [ dbSNP | Ensembl ].

VAR_004355

Natural variant

86

1

E → G in allele A*24:08.

VAR_004356

Natural variant

89

1

G → R in allele A*24:08 and allele A*24:29.

VAR_004357

Natural variant

119

1

L → V in allele A*24:14.

VAR_015765

Natural variant

121

1

M → R in allele A*24:14.

VAR_015766

Natural variant

123

1

F → Y in allele A*24:14.

VAR_015767

Natural variant

131

1

G → W in allele A*24:14.

VAR_015768

Natural variant

180

1

Q → L in allele A*24:13.

VAR_015769

Natural variant

180

1

Q → W in allele A*24:06; requires 2 nucleotide substitutions. Ref.14

VAR_004358

Natural variant

187

1

T → R in allele A*24:10.

VAR_015770

Natural variant

190 – 191

2

DG → EW in allele A*24:03 and allele A*24:10.

VAR_004359

Natural variant

205

1

R → H.
Corresponds to variant rs17185861 [ dbSNP | Ensembl ].

VAR_056263

Natural variant

206

1

T → A in allele A*2401.

VAR_004360

Secondary structure

1

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365

Helix Strand Turn

Details...

Beta strand

27 – 36

10

Turn

39 – 41

3

Beta strand

45 – 52

8

Beta strand

55 – 61

7

Beta strand

64 – 66

3

Beta strand

70 – 73

4

Helix

74 – 78

5

Helix

81 – 109

29

Beta strand

118 – 127

10

Beta strand

133 – 142

10

Beta strand

145 – 150

6

Beta strand

157 – 159

3

Helix

162 – 173

12

Helix

176 – 185

10

Helix

187 – 198

12

Helix

200 – 203

4

Beta strand

210 – 217

8

Beta strand

219 – 235

17

Beta strand

238 – 246

9

Helix

249 – 251

3

Beta strand

252 – 254

3

Beta strand

261 – 263

3

Beta strand

265 – 274

10

Beta strand

280 – 286

7

Beta strand

290 – 292

3

Beta strand

294 – 296

3

Beta strand

298 – 300

3

Sequences

	Sequence		Length	Mass (Da)
	P05534 [UniParc]. Last modified June 27, 2003. Version 2. Checksum: D33684D126F98EC3	FASTA	365	40,689
	10 20 30 40 50 60 MAVMAPRTLV LLLSGALALT QTWAGSHSMR YFSTSVSRPG RGEPRFIAVG YVDDTQFVRF 70 80 90 100 110 120 DSDAASQRME PRAPWIEQEG PEYWDEETGK VKAHSQTDRE NLRIALRYYN QSEAGSHTLQ 130 140 150 160 170 180 MMFGCDVGSD GRFLRGYHQY AYDGKDYIAL KEDLRSWTAA DMAAQITKRK WEAAHVAEQQ 190 200 210 220 230 240 RAYLEGTCVD GLRRYLENGK ETLQRTDPPK THMTHHPISD HEATLRCWAL GFYPAEITLT 250 260 270 280 290 300 WQRDGEDQTQ DTELVETRPA GDGTFQKWAA VVVPSGEEQR YTCHVQHEGL PKPLTLRWEP 310 320 330 340 350 360 SSQPTVPIVG IIAGLVLLGA VITGAVVAAV MWRRNSSDRK GGSYSQAASS DSAQGSDVSL TACKV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The HLA-AW24 gene: sequence, surroundings and comparison with the HLA-A2 and HLA-A3 genes." N'Guyen C., Sodoyer R., Trucy J., Strachan T., Jordan B.R. Immunogenetics 21:479-489(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ALLELE A*2401).
[2]	Jordan B.R. Submitted (JUL-1988) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[3]	"Molecular definition of an elusive third HLA-A9 molecule: HLA-A9.3." Little A.-M., Madrigal J.A., Parham P. Immunogenetics 35:41-45(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ALLELES A*24:02 AND A*24:03).
[4]	"Unusual HLA-B alleles in two tribes of Brazilian Indians." Belich M.P., Madrigal J.A., Hildebrand W.H., Zemmour J., Williams R.C., Luz R., Petzl-Erler M.L., Parham P. Nature 357:326-329(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE (ALLELE A*24:02).
[5]	"An intronic mutation responsible for a low level of expression of an HLA-A*24 allele." Laforet M., Froelich N., Parissiadis A., Bausinger H., Pfeiffer B., Tongio M.M. Tissue Antigens 50:340-346(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ALLELE A*24:02).
[6]	"Sequence analysis of a serological subtype, HLA-A9HH, observed in Japanese and the confirmatory sequence of A*2408." Kashiwase K., Tokunaga K., Ishikawa Y., Qiu L., Furuya M., Sawanaka K., Akaza T., Tadokoro K., Juji T. MHC 3:9-14(1996) Cited for: NUCLEOTIDE SEQUENCE (ALLELE A*24:08). Tissue: Blood.
[7]	"Confirmation of the sequence of HLA-A*2429 allele found in a potential bone marrow donor." Dunn P. Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 26-337 (ALLELE A*24:29).
[8]	"A novel HLA-A*24 (A*2410) identified in a Javanese population." Gao X., Matheson B. Tissue Antigens 48:711-713(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 26-206 (ALLELE A*24:10). Tissue: Blood.
[9]	"Three newly identified A*24 alleles: A*2406, A*2413 and A*2414." Gao X., Lester S., Matheson B., Boettcher B., McCluskey J. Tissue Antigens 50:192-196(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 26-206 (ALLELES A*24:06; A*24:13 AND A*24:14). Tissue: Blood.
[10]	"Phosphoproteome of resting human platelets." Zahedi R.P., Lewandrowski U., Wiesner J., Wortelkamp S., Moebius J., Schuetz C., Walter U., Gambaryan S., Sickmann A. J. Proteome Res. 7:526-534(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-356, MASS SPECTROMETRY. Tissue: Platelet.
[11]	"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[12]	"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry." Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H. J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-110, MASS SPECTROMETRY. Tissue: Liver.
[13]	"Plasticity of human CD8alphaalpha binding to peptide-HLA-A*2402." Shi Y., Qi J., Iwamoto A., Gao G.F. Mol. Immunol. 48:2198-2202(2011) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.39 ANGSTROMS) OF 25-298 (ALLELE A*24:02) IN COMPLEX WITH B2M AND CD8, DISULFIDE BONDS.
[14]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] TRP-180, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M15497 Genomic DNA. Translation: AAA59611.1.
M64740 mRNA. Translation: AAA59600.1.
M64741 Genomic DNA. Translation: AAA59601.1.
Z72422 Genomic DNA. Translation: CAA96532.1.
D83516 mRNA. Translation: BAA11936.1.
U37111, U37110 Genomic DNA. Translation: AAA83264.1.
U37113, U37112 Genomic DNA. Translation: AAA83265.1.
AH004915 Genomic DNA. Translation: AAB40048.1.
AH005378 Genomic DNA. Translation: AAB60651.1.

IPI

IPI00742968.

PIR

I54416.

UniGene

Hs.181244.
Hs.713441.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2BCK	X-ray	2.80	A/D	25-300	[»]
3I6L	X-ray	2.40	D	25-298	[»]
3NFN	X-ray	2.39	A	25-298	[»]
3QZW	X-ray	2.80	A/D	25-298	[»]
4F7M	X-ray	2.40	A/D	25-298	[»]
4F7P	X-ray	1.90	A	25-298	[»]
4F7T	X-ray	1.70	A/D	25-298	[»]

ProteinModelPortal

P05534.

SMR

P05534. Positions 25-298.

ModBase

Search...

Polymorphism databases

DMDM

32363482.

Proteomic databases

PaxDb

P05534.

PRIDE

P05534.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000431930; ENSP00000406366; ENSG00000229215.
ENST00000443552; ENSP00000404678; ENSG00000224320.

Organism-specific databases

GeneCards

GC06P029933.

H-InvDB

HIX0165910.
HIX0165954.
HIX0166079.
HIX0166152.
HIX0166432.
HIX0166685.
HIX0166954.

HGNC

HGNC:4931. HLA-A.

MIM

142800. gene.

neXtProt

NX_P05534.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG42056.

HOVERGEN

HBG016709.

Enzyme and pathway databases

Reactome

REACT_6900. Immune System.

Gene expression databases

CleanEx

HS_HLA-A.

Genevestigator

P05534.

Family and domain databases

Gene3D

2.60.40.10. 1 hit.
3.30.500.10. 1 hit.

InterPro

IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003006. Ig/MHC_CS.
IPR003597. Ig_C1-set.
IPR011161. MHC_I-like_Ag-recog.
IPR011162. MHC_I/II-like_Ag-recog.
IPR027648. MHC_I_a.
IPR001039. MHC_I_a_a1/a2.
IPR010579. MHC_I_a_C.
[Graphical view]

Pfam

PF07654. C1-set. 1 hit.
PF00129. MHC_I. 1 hit.
PF06623. MHC_I_C. 1 hit.
[Graphical view]

PRINTS

PR01638. MHCCLASSI.

SMART

SM00407. IGc1. 1 hit.
[Graphical view]

SUPFAM

SSF54452. SSF54452. 1 hit.

PROSITE

PS50835. IG_LIKE. 1 hit.
PS00290. IG_MHC. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

HLA-A. human.

EvolutionaryTrace

P05534.

PRO

P05534.

SOURCE

Search...

Entry information

Entry name

1A24_HUMAN

Accession

Primary (citable) accession number: P05534
Secondary accession number(s): P30448 , P30449, Q29908, Q29909, Q29910, Q95355

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1988
Last sequence update:	June 27, 2003
Last modified:	November 13, 2013
This is version 141 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.