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Protein

Lymphocyte antigen 6A-2/6E-1

Gene

Ly6a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

T-cell activation.

Names & Taxonomyi

Protein namesi
Recommended name:
Lymphocyte antigen 6A-2/6E-1
Short name:
Ly-6A.2/Ly-6E.1
Alternative name(s):
Stem cell antigen 1
Short name:
SCA-1
T-cell-activating protein
Short name:
TAP
Gene namesi
Name:Ly6a
Synonyms:Ly6
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:107527. Ly6a.

Subcellular locationi

GO - Cellular componenti

  • anchored component of membrane Source: UniProtKB-KW
  • external side of plasma membrane Source: MGI
  • intracellular Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 26261 PublicationAdd
BLAST
Chaini27 – 11286Lymphocyte antigen 6A-2/6E-1PRO_0000036130Add
BLAST
Propeptidei113 – 13422Removed in mature formSequence analysisPRO_0000036131Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi29 ↔ 53By similarity
Disulfide bondi32 ↔ 41By similarity
Disulfide bondi46 ↔ 74By similarity
Disulfide bondi78 ↔ 98By similarity
Disulfide bondi99 ↔ 104By similarity
Lipidationi112 – 1121GPI-anchor amidated glycineSequence analysis

Post-translational modificationi

O-glycosylated. Not N-glycosylated.1 Publication
Not phosphorylated.

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

EPDiP05533.
MaxQBiP05533.
PaxDbiP05533.
PeptideAtlasiP05533.
PRIDEiP05533.

Expressioni

Tissue specificityi

Widely expressed.2 Publications

Gene expression databases

BgeeiP05533.
CleanExiMM_LY6A.
ExpressionAtlasiP05533. baseline and differential.
GenevisibleiP05533. MM.

Interactioni

Protein-protein interaction databases

DIPiDIP-59628N.
IntActiP05533. 1 interaction.
STRINGi10090.ENSMUSP00000023248.

Structurei

3D structure databases

ProteinModelPortaliP05533.
SMRiP05533. Positions 42-108.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini27 – 11993UPAR/Ly6Add
BLAST

Sequence similaritiesi

Contains 1 UPAR/Ly6 domain.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410JI0Q. Eukaryota.
ENOG41117PH. LUCA.
GeneTreeiENSGT00730000111651.
HOGENOMiHOG000035114.
HOVERGENiHBG001716.
InParanoidiP05533.
KOiK06846.
OMAiNIESMEI.
OrthoDBiEOG7XDBJQ.
PhylomeDBiP05533.
TreeFamiTF337757.

Family and domain databases

InterProiIPR018363. CD59_antigen_CS.
IPR016054. LY6_UPA_recep-like.
[Graphical view]
PfamiPF00021. UPAR_LY6. 1 hit.
[Graphical view]
SMARTiSM00134. LU. 1 hit.
[Graphical view]
PROSITEiPS00983. LY6_UPAR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P05533-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDTSHTTKSC LLILLVALLC AERAQGLECY QCYGVPFETS CPSITCPYPD
60 70 80 90 100
GVCVTQEAAV IVDSQTRKVK NNLCLPICPP NIESMEILGT KVNVKTSCCQ
110 120 130
EDLCNVAVPN GGSTWTMAGV LLFSLSSVLL QTLL
Length:134
Mass (Da):14,377
Last modified:November 1, 1988 - v1
Checksum:i883C771EB3430315
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti32 – 321C → S in BAC36250 (PubMed:16141072).Curated
Sequence conflicti56 – 561Q → H in BAC36250 (PubMed:16141072).Curated
Sequence conflicti59 – 591A → T in BAC36250 (PubMed:16141072).Curated
Sequence conflicti101 – 1011E → Q in BAC36250 (PubMed:16141072).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti63 – 631D → G in Ly-6E.1.
Natural varianti106 – 1061V → A in Ly-6E.1.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M18184 mRNA. Translation: AAA39463.1.
J03636 mRNA. Translation: AAA40163.1.
X04653 mRNA. Translation: CAA28351.1.
M37707 Genomic DNA. Translation: AAA39467.1.
M74013 Genomic DNA. Translation: AAA39464.1.
M73552 Genomic DNA. Translation: AAA39465.1.
M59713 Genomic DNA. Translation: AAA40164.1.
AK076200 mRNA. Translation: BAC36250.1.
BC002070 mRNA. Translation: AAH02070.1.
CCDSiCCDS27540.1.
PIRiA25708.
A35921. A32506.
RefSeqiNP_001258345.1. NM_001271416.1.
NP_001258346.1. NM_001271417.1.
NP_001258347.1. NM_001271418.1.
NP_001258348.1. NM_001271419.1.
NP_001258375.1. NM_001271446.1.
NP_034868.1. NM_010738.3.
UniGeneiMm.425949.

Genome annotation databases

EnsembliENSMUST00000023248; ENSMUSP00000023248; ENSMUSG00000075602.
ENSMUST00000186526; ENSMUSP00000140998; ENSMUSG00000075602.
ENSMUST00000187171; ENSMUSP00000140099; ENSMUSG00000075602.
ENSMUST00000187994; ENSMUSP00000140287; ENSMUSG00000075602.
ENSMUST00000189068; ENSMUSP00000140638; ENSMUSG00000075602.
GeneIDi110454.
KEGGimmu:110454.
UCSCiuc007wgk.2. mouse.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M18184 mRNA. Translation: AAA39463.1.
J03636 mRNA. Translation: AAA40163.1.
X04653 mRNA. Translation: CAA28351.1.
M37707 Genomic DNA. Translation: AAA39467.1.
M74013 Genomic DNA. Translation: AAA39464.1.
M73552 Genomic DNA. Translation: AAA39465.1.
M59713 Genomic DNA. Translation: AAA40164.1.
AK076200 mRNA. Translation: BAC36250.1.
BC002070 mRNA. Translation: AAH02070.1.
CCDSiCCDS27540.1.
PIRiA25708.
A35921. A32506.
RefSeqiNP_001258345.1. NM_001271416.1.
NP_001258346.1. NM_001271417.1.
NP_001258347.1. NM_001271418.1.
NP_001258348.1. NM_001271419.1.
NP_001258375.1. NM_001271446.1.
NP_034868.1. NM_010738.3.
UniGeneiMm.425949.

3D structure databases

ProteinModelPortaliP05533.
SMRiP05533. Positions 42-108.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-59628N.
IntActiP05533. 1 interaction.
STRINGi10090.ENSMUSP00000023248.

Proteomic databases

EPDiP05533.
MaxQBiP05533.
PaxDbiP05533.
PeptideAtlasiP05533.
PRIDEiP05533.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000023248; ENSMUSP00000023248; ENSMUSG00000075602.
ENSMUST00000186526; ENSMUSP00000140998; ENSMUSG00000075602.
ENSMUST00000187171; ENSMUSP00000140099; ENSMUSG00000075602.
ENSMUST00000187994; ENSMUSP00000140287; ENSMUSG00000075602.
ENSMUST00000189068; ENSMUSP00000140638; ENSMUSG00000075602.
GeneIDi110454.
KEGGimmu:110454.
UCSCiuc007wgk.2. mouse.

Organism-specific databases

CTDi110454.
MGIiMGI:107527. Ly6a.

Phylogenomic databases

eggNOGiENOG410JI0Q. Eukaryota.
ENOG41117PH. LUCA.
GeneTreeiENSGT00730000111651.
HOGENOMiHOG000035114.
HOVERGENiHBG001716.
InParanoidiP05533.
KOiK06846.
OMAiNIESMEI.
OrthoDBiEOG7XDBJQ.
PhylomeDBiP05533.
TreeFamiTF337757.

Miscellaneous databases

PROiP05533.
SOURCEiSearch...

Gene expression databases

BgeeiP05533.
CleanExiMM_LY6A.
ExpressionAtlasiP05533. baseline and differential.
GenevisibleiP05533. MM.

Family and domain databases

InterProiIPR018363. CD59_antigen_CS.
IPR016054. LY6_UPA_recep-like.
[Graphical view]
PfamiPF00021. UPAR_LY6. 1 hit.
[Graphical view]
SMARTiSM00134. LU. 1 hit.
[Graphical view]
PROSITEiPS00983. LY6_UPAR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "cDNA characterization of an Ly-6.2 gene expressed in BW5147 tumor cells."
    Palfree R.G.E., Leclair K.P., Bothwell A.L.M., Haemmerling U.
    Immunogenetics 26:389-391(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Cloning and expression of a cDNA for the T-cell-activating protein TAP."
    Reiser H., Coligan J., Benacerraf B., Rock K.L.
    Proc. Natl. Acad. Sci. U.S.A. 85:2255-2259(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Isolation of a murine Ly-6 cDNA reveals a new multigene family."
    Leclair K.P., Palfree R.G.E., Flood P.M., Hammerling U., Bothwell A.L.M.
    EMBO J. 5:3227-3234(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (LY-6E.1).
  4. "Characterization of promoter elements of an interferon-inducible Ly-6E/A differentiation antigen, which is expressed on activated T cells and hematopoietic stem cells."
    Khan K., Dad Lindwall G., Maher S.E., Bothwell A.L.M.
    Mol. Cell. Biol. 10:5150-5159(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: BALB/cJ.
  5. "The isolation and sequence of the chromosomal gene and regulatory regions of Ly-6A.2."
    Stanford W.L., Bruyns E., Snodgrass H.R.
    Immunogenetics 35:408-411(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  6. "Isolation, expression, and sequence of the TAP/Ly-6A.2 chromosomal gene."
    McGrew J.T., Rock K.L.
    J. Immunol. 146:3633-3638(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  7. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Extraembryonic tissue and Placenta.
  8. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Mammary gland.
  9. "Biosynthesis, glycosylation, and partial N-terminal amino acid sequence of the T-cell-activating protein TAP."
    Reiser H., Coligan J., Benacerraf B., Rock K.L.
    Proc. Natl. Acad. Sci. U.S.A. 84:3370-3374(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 27-49, GLYCOSYLATION.
  10. "Mouse hematopoietic stem-cell antigen Sca-1 is a member of the Ly-6 antigen family."
    van de Rijn M., Heimfeld S., Spangrude G.J., Weissman I.L.
    Proc. Natl. Acad. Sci. U.S.A. 86:4634-4638(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  11. Cited for: TISSUE SPECIFICITY.
  12. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brown adipose tissue, Heart, Kidney, Lung and Spleen.

Entry informationi

Entry nameiLY6A_MOUSE
AccessioniPrimary (citable) accession number: P05533
Secondary accession number(s): Q8BPD0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: November 1, 1988
Last modified: July 6, 2016
This is version 139 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.