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P05532

- KIT_MOUSE

UniProt

P05532 - KIT_MOUSE

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Protein

Mast/stem cell growth factor receptor Kit

Gene
Kit, Sl
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1.7 Publications

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.1 Publication

Enzyme regulationi

Present in an inactive conformation in the absence of bound ligand. KITLG/SCF binding leads to dimerization and activation by autophosphorylation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi571 – 5711Magnesium By similarity
Binding sitei626 – 6261ATP By similarity
Active sitei794 – 7941Proton acceptor By similarity
Binding sitei798 – 7981ATP By similarity
Metal bindingi799 – 7991Magnesium By similarity
Metal bindingi812 – 8121Magnesium By similarity
Sitei938 – 9381Important for interaction with phosphotyrosine-binding proteins By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi599 – 6068ATP By similarity
Nucleotide bindingi674 – 6807ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. cytokine binding Source: UniProtKB
  3. metal ion binding Source: UniProtKB-KW
  4. protease binding Source: BHF-UCL
  5. protein binding Source: MGI
  6. receptor signaling protein tyrosine kinase activity Source: InterPro
  7. stem cell factor receptor activity Source: MGI
  8. transmembrane receptor protein tyrosine kinase activity Source: UniProtKB

GO - Biological processi

  1. actin cytoskeleton reorganization Source: UniProtKB
  2. activation of MAPK activity Source: Ensembl
  3. cell chemotaxis Source: UniProtKB
  4. cell differentiation Source: MGI
  5. cellular response to thyroid hormone stimulus Source: UniProtKB
  6. chemotaxis Source: MGI
  7. cytokine-mediated signaling pathway Source: MGI
  8. dendritic cell cytokine production Source: UniProtKB
  9. detection of mechanical stimulus involved in sensory perception of sound Source: UniProtKB
  10. developmental pigmentation Source: MGI
  11. digestive tract development Source: UniProtKB
  12. ectopic germ cell programmed cell death Source: MGI
  13. embryonic hemopoiesis Source: UniProtKB
  14. epithelial cell proliferation Source: Ensembl
  15. erythrocyte differentiation Source: UniProtKB
  16. erythropoietin-mediated signaling pathway Source: UniProtKB
  17. Fc receptor signaling pathway Source: UniProtKB
  18. germ cell development Source: MGI
  19. germ cell migration Source: Ensembl
  20. glycosphingolipid metabolic process Source: MGI
  21. hemopoiesis Source: MGI
  22. immature B cell differentiation Source: UniProtKB
  23. inflammatory response Source: UniProtKB
  24. intracellular signal transduction Source: MGI
  25. lamellipodium assembly Source: UniProtKB
  26. lymphoid progenitor cell differentiation Source: MGI
  27. male gonad development Source: Ensembl
  28. mast cell chemotaxis Source: Ensembl
  29. mast cell cytokine production Source: UniProtKB
  30. mast cell degranulation Source: UniProtKB
  31. mast cell differentiation Source: UniProtKB
  32. megakaryocyte development Source: UniProtKB
  33. melanocyte adhesion Source: UniProtKB
  34. melanocyte differentiation Source: UniProtKB
  35. melanocyte migration Source: UniProtKB
  36. myeloid leukocyte differentiation Source: MGI
  37. myeloid progenitor cell differentiation Source: MGI
  38. negative regulation of programmed cell death Source: MGI
  39. ovarian follicle development Source: UniProtKB
  40. peptidyl-tyrosine phosphorylation Source: MGI
  41. pigmentation Source: UniProtKB
  42. positive regulation of cell migration Source: Ensembl
  43. positive regulation of cell proliferation Source: MGI
  44. positive regulation of gene expression Source: MGI
  45. positive regulation of long-term neuronal synaptic plasticity Source: Ensembl
  46. positive regulation of MAP kinase activity Source: MGI
  47. positive regulation of Notch signaling pathway Source: Ensembl
  48. positive regulation of pseudopodium assembly Source: Ensembl
  49. positive regulation of sequence-specific DNA binding transcription factor activity Source: Ensembl
  50. positive regulation of tyrosine phosphorylation of Stat1 protein Source: Ensembl
  51. positive regulation of tyrosine phosphorylation of Stat3 protein Source: Ensembl
  52. positive regulation of tyrosine phosphorylation of Stat5 protein Source: Ensembl
  53. protein autophosphorylation Source: MGI
  54. protein phosphorylation Source: MGI
  55. regulation of cell shape Source: UniProtKB
  56. regulation of developmental pigmentation Source: MGI
  57. response to radiation Source: MGI
  58. somatic stem cell division Source: Ensembl
  59. somatic stem cell maintenance Source: Ensembl
  60. spermatid development Source: MGI
  61. spermatogenesis Source: UniProtKB
  62. stem cell differentiation Source: UniProtKB
  63. T cell differentiation Source: UniProtKB
  64. visual learning Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Receptor, Transferase, Tyrosine-protein kinase

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.10.1. 3474.
ReactomeiREACT_188578. Signaling by SCF-KIT.
REACT_196588. Constitutive PI3K/AKT Signaling in Cancer.
REACT_226341. PIP3 activates AKT signaling.
REACT_227425. Regulation of KIT signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
Mast/stem cell growth factor receptor Kit (EC:2.7.10.1)
Short name:
SCFR
Alternative name(s):
Proto-oncogene c-Kit
Tyrosine-protein kinase Kit
CD_antigen: CD117
Gene namesi
Name:Kit
Synonyms:Sl
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 5

Organism-specific databases

MGIiMGI:96677. Kit.

Subcellular locationi

Isoform 1 : Cell membrane; Single-pass type I membrane protein 1 Publication
Isoform 2 : Cell membrane; Single-pass type I membrane protein 1 Publication
Isoform 3 : Cytoplasm
Note: Detected in the cytoplasm of spermatozoa, especially in the equatorial and subacrosomal region of the sperm head By similarity.1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini25 – 527503Extracellular Reviewed predictionAdd
BLAST
Transmembranei528 – 54821Helical; Reviewed predictionAdd
BLAST
Topological domaini549 – 979431Cytoplasmic Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. acrosomal vesicle Source: Ensembl
  2. cell-cell junction Source: MGI
  3. cell surface Source: MGI
  4. cytoplasmic side of plasma membrane Source: Ensembl
  5. external side of plasma membrane Source: MGI
  6. extracellular space Source: Ensembl
  7. integral component of membrane Source: UniProtKB-KW
  8. mast cell granule Source: GOC
  9. nucleus Source: Ensembl
  10. plasma membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Involvement in diseasei

Defects in Kit are the cause of the white-spotting phenotype (W). White-spotting variants induces severe effects on pigmentation, gametogenesis and hematopoiesis. Mice homozygous for W42 die perinatally of macrocytic anemia.8 Publications

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi571 – 5711Y → F: Abolishes interaction with PTPN11/SHP-2. 1 Publication
Mutagenesisi573 – 5731Y → F: Abolishes interaction with PTPN6/SHP-1. 1 Publication
Mutagenesisi573 – 5731Missing: Abolishes interaction with PTPN6/SHP-1. 1 Publication
Mutagenesisi723 – 7231Y → F: Abolishes interaction with PIK3R1. 1 Publication
Mutagenesisi860 – 8601F → S: Mice display white fur, hearing loss, anemia and mast cell deficiency, plus sterility in both males and females. 1 Publication

Keywords - Diseasei

Disease mutation, Proto-oncogene

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424 Reviewed predictionAdd
BLAST
Chaini25 – 979955Mast/stem cell growth factor receptor KitPRO_0000016755Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi58 ↔ 981 Publication
Disulfide bondi137 ↔ 1871 Publication
Glycosylationi146 – 1461N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi152 ↔ 1841 Publication
Disulfide bondi234 ↔ 2931 Publication
Glycosylationi296 – 2961N-linked (GlcNAc...)1 Publication
Glycosylationi303 – 3031N-linked (GlcNAc...)1 Publication
Glycosylationi323 – 3231N-linked (GlcNAc...) Reviewed prediction
Glycosylationi355 – 3551N-linked (GlcNAc...) Reviewed prediction
Glycosylationi370 – 3701N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi431 ↔ 494 By similarity
Glycosylationi466 – 4661N-linked (GlcNAc...) Reviewed prediction
Glycosylationi489 – 4891N-linked (GlcNAc...) Reviewed prediction
Modified residuei550 – 5501Phosphotyrosine; by autocatalysis By similarity
Modified residuei556 – 5561Phosphotyrosine; by autocatalysis By similarity
Modified residuei571 – 5711Phosphotyrosine; alternate1 Publication
Modified residuei571 – 5711Phosphotyrosine; by autocatalysis; alternate By similarity
Modified residuei573 – 5731Phosphotyrosine; alternate1 Publication
Modified residuei573 – 5731Phosphotyrosine; by autocatalysis; alternate By similarity
Modified residuei706 – 7061Phosphotyrosine; alternate1 Publication
Modified residuei706 – 7061Phosphotyrosine; by autocatalysis; alternate By similarity
Modified residuei723 – 7231Phosphotyrosine; by autocatalysis By similarity
Modified residuei732 – 7321Phosphotyrosine; by autocatalysis By similarity
Modified residuei743 – 7431Phosphoserine; by PKC/PRKCA By similarity
Modified residuei748 – 7481Phosphoserine; by PKC/PRKCA By similarity
Modified residuei823 – 8231Phosphoserine By similarity
Modified residuei825 – 8251Phosphotyrosine; by autocatalysis1 Publication
Modified residuei893 – 8931Phosphoserine By similarity
Modified residuei902 – 9021Phosphotyrosine; by autocatalysis By similarity
Modified residuei938 – 9381Phosphotyrosine; alternate1 Publication
Modified residuei938 – 9381Phosphotyrosine; by autocatalysis; alternate By similarity
Modified residuei962 – 9621Phosphoserine By similarity

Post-translational modificationi

Ubiquitinated by SOCS6. KIT is rapidly ubiquitinated after autophosphorylation induced by KITLG/SCF binding, leading to internalization and degradation By similarity.1 Publication
Autophosphorylated on tyrosine residues. KITLG/SCF binding promotes autophosphorylation of isoform 1 and isoform 2. Isoform 1 shows low levels of tyrosine phosphorylation in the absence of added KITLG/SCF, while isoform 2 requires stimulation by KITLG/SCF for phosphorylation (in vitro). Phosphorylation of Tyr-573 is required for interaction with PTPN6/SHP-1. Phosphorylation of Tyr-571 is required for interaction with PTPN11/SHP-2. Phosphorylated tyrosine residues are important for interaction with specific binding partners.2 Publications

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP05532.
PaxDbiP05532.
PRIDEiP05532.

PTM databases

PhosphoSiteiP05532.

Expressioni

Tissue specificityi

Isoform 1 and isoform 2 are detected in bone marrow cells, spermatogonia and spermatocytes, but not in round spermatids, elongating spermatids and spermatozoa. Isoform 3 is detected in round spermatids, elongating spermatids and spermatozoa, but not in spermatogonia and spermatocytes (at protein level). Isoform 1 is widely expressed and detected in fetal liver and bone marrow. Isoform 3 is detected in bone marrow cells enriched in hematopoietic stem cells.3 Publications

Gene expression databases

ArrayExpressiP05532.
BgeeiP05532.
CleanExiMM_KIT.
GenevestigatoriP05532.

Interactioni

Subunit structurei

Monomer in the absence of bound KITLG/SCF. Homodimer in the presence of bound KITLG/SCF, forming a heterotetramer with two KITLG/SCF molecules. Interacts (via phosphorylated tyrosine residues) with the adapter proteins GRB2 and GRB7 (via SH2 domain), and SH2B2/APS. Interacts (via C-terminus) with MPDZ (via the tenth PDZ domain). Interacts (via phosphorylated tyrosine residues) with the protein phosphatases PTPN6/SHP-1 (via SH2 domain), PTPN11/SHP-2 (via SH2 domain) and PTPRU. Interacts with DOK1 and TEC By similarity. Interacts with the protein kinase FES/FPS. Interacts with PLCG1. Interacts (via phosphorylated tyrosine residues) with PIK3R1 and PIK3 catalytic subunit.6 Publications

Protein-protein interaction databases

BioGridi200957. 18 interactions.
DIPiDIP-59622N.
IntActiP05532. 3 interactions.

Structurei

Secondary structure

1
979
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi38 – 414
Beta strandi43 – 497
Beta strandi54 – 596
Beta strandi63 – 697
Beta strandi71 – 733
Beta strandi75 – 784
Beta strandi80 – 856
Helixi90 – 923
Beta strandi94 – 10310
Beta strandi105 – 1139
Beta strandi125 – 1284
Beta strandi133 – 1353
Beta strandi145 – 1517
Beta strandi162 – 1665
Turni167 – 1693
Beta strandi170 – 1756
Helixi178 – 1803
Beta strandi184 – 1918
Beta strandi194 – 1974
Beta strandi201 – 2066
Beta strandi214 – 2163
Beta strandi230 – 24011
Beta strandi244 – 25613
Beta strandi264 – 2685
Beta strandi271 – 28212
Beta strandi285 – 2873
Beta strandi289 – 2968
Beta strandi301 – 3088

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2O26X-ray2.50U/W/X/Y25-314[»]
ProteinModelPortaliP05532.
SMRiP05532. Positions 33-511, 550-933.

Miscellaneous databases

EvolutionaryTraceiP05532.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini31 – 11787Ig-like C2-type 1Add
BLAST
Domaini126 – 21085Ig-like C2-type 2Add
BLAST
Domaini217 – 31599Ig-like C2-type 3Add
BLAST
Domaini324 – 41794Ig-like C2-type 4Add
BLAST
Domaini420 – 51495Ig-like C2-type 5Add
BLAST
Domaini592 – 939348Protein kinaseAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni571 – 5733Important for interaction with phosphotyrosine-binding proteins By similarity

Sequence similaritiesi

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00720000108490.
HOGENOMiHOG000112008.
HOVERGENiHBG004335.
InParanoidiQ6QJB7.
KOiK05091.
TreeFamiTF325768.

Family and domain databases

Gene3Di2.60.40.10. 5 hits.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR027263. SCGF_receptor.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
IPR016243. Tyr_kinase_CSF1/PDGF_rcpt.
IPR001824. Tyr_kinase_rcpt_3_CS.
[Graphical view]
PfamiPF07679. I-set. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
PIRSFiPIRSF500951. SCGF_recepter. 1 hit.
PIRSF000615. TyrPK_CSF1-R. 1 hit.
SMARTiSM00409. IG. 2 hits.
SM00408. IGc2. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 2 hits.
PROSITEiPS50835. IG_LIKE. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS00240. RECEPTOR_TYR_KIN_III. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P05532-1) [UniParc]FASTAAdd to Basket

Also known as: GNNK(+), Kit(+)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MRGARGAWDL LCVLLVLLRG QTATSQPSAS PGEPSPPSIH PAQSELIVEA    50
GDTLSLTCID PDFVRWTFKT YFNEMVENKK NEWIQEKAEA TRTGTYTCSN 100
SNGLTSSIYV FVRDPAKLFL VGLPLFGKED SDALVRCPLT DPQVSNYSLI 150
ECDGKSLPTD LTFVPNPKAG ITIKNVKRAY HRLCVRCAAQ RDGTWLHSDK 200
FTLKVRAAIK AIPVVSVPET SHLLKKGDTF TVVCTIKDVS TSVNSMWLKM 250
NPQPQHIAQV KHNSWHRGDF NYERQETLTI SSARVDDSGV FMCYANNTFG 300
SANVTTTLKV VEKGFINISP VKNTTVFVTD GENVDLVVEY EAYPKPEHQQ 350
WIYMNRTSAN KGKDYVKSDN KSNIRYVNQL RLTRLKGTEG GTYTFLVSNS 400
DASASVTFNV YVNTKPEILT YDRLINGMLQ CVAEGFPEPT IDWYFCTGAE 450
QRCTTPVSPV DVQVQNVSVS PFGKLVVQSS IDSSVFRHNG TVECKASNDV 500
GKSSAFFNFA FKGNNKEQIQ AHTLFTPLLI GFVVAAGAMG IIVMVLTYKY 550
LQKPMYEVQW KVVEEINGNN YVYIDPTQLP YDHKWEFPRN RLSFGKTLGA 600
GAFGKVVEAT AYGLIKSDAA MTVAVKMLKP SAHLTEREAL MSELKVLSYL 650
GNHMNIVNLL GACTVGGPTL VITEYCCYGD LLNFLRRKRD SFIFSKQEEQ 700
AEAALYKNLL HSTEPSCDSS NEYMDMKPGV SYVVPTKTDK RRSARIDSYI 750
ERDVTPAIME DDELALDLDD LLSFSYQVAK GMAFLASKNC IHRDLAARNI 800
LLTHGRITKI CDFGLARDIR NDSNYVVKGN ARLPVKWMAP ESIFSCVYTF 850
ESDVWSYGIF LWELFSLGSS PYPGMPVDSK FYKMIKEGFR MVSPEHAPAE 900
MYDVMKTCWD ADPLKRPTFK QVVQLIEKQI SDSTKHIYSN LANCNPNPEN 950
PVVVDHSVRV NSVGSSASST QPLLVHEDA 979
Length:979
Mass (Da):109,343
Last modified:October 19, 2011 - v3
Checksum:i03FB4D672248585E
GO
Isoform 2 (identifier: P05532-2) [UniParc]FASTAAdd to Basket

Also known as: GNNK(-), KitA(+)

The sequence of this isoform differs from the canonical sequence as follows:
     512-515: Missing.

Show »
Length:975
Mass (Da):108,929
Checksum:i57BFE10AE456C145
GO
Isoform 3 (identifier: P05532-3) [UniParc]FASTAAdd to Basket

Also known as: Tr-kit, Truncated

The sequence of this isoform differs from the canonical sequence as follows:
     1-12: MRGARGAWDLLC → MAVAVFPFLPQQ
     13-789: Missing.

Show »
Length:202
Mass (Da):22,814
Checksum:i38323E3690025842
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti207 – 2071A → E Loss-of-function mutation abolishing ligand binding. 2 Publications
Natural varianti586 – 5861E → K in W37 spotting; impaired protein stability and loss of kinase activity. 2 Publications
Natural varianti664 – 6641T → M in Wv spotting. 1 Publication
Natural varianti794 – 7941D → N in W42 spotting; loss of kinase activity and impaired internalization after exposure to KITLG/SCF. 2 Publications
Natural varianti835 – 8351V → M in W41 spotting; decreased kinase activity. 2 Publications

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 1212MRGAR…WDLLC → MAVAVFPFLPQQ in isoform 3. VSP_041868Add
BLAST
Alternative sequencei13 – 789777Missing in isoform 3. VSP_041869Add
BLAST
Alternative sequencei512 – 5154Missing in isoform 2. VSP_041870

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti551 – 5511L → F in AAH52457. 1 Publication
Sequence conflicti781 – 7811G → A in CAA68772. 1 Publication
Sequence conflicti781 – 7811G → A in CAA46799. 1 Publication
Sequence conflicti860 – 8601F → S in AAS45607. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y00864 mRNA. Translation: CAA68772.1.
AK046795 mRNA. Translation: BAC32872.1.
X65997 mRNA. Translation: CAA46798.1.
X65998 Genomic DNA. Translation: CAA46799.1. Sequence problems.
X65998 Genomic DNA. Translation: CAA46800.1.
AY536430 mRNA. Translation: AAS45606.1.
AY536431 mRNA. Translation: AAS45607.1.
AC013622 Genomic DNA. No translation available.
AC115853 Genomic DNA. No translation available.
BC052457 mRNA. Translation: AAH52457.1.
BC075716 mRNA. Translation: AAH75716.1.
L11358 Genomic DNA. Translation: AAA37420.1.
CCDSiCCDS51525.1. [P05532-1]
PIRiA44876.
S00474. TVMSKT.
S24667.
S34435.
RefSeqiNP_001116205.1. NM_001122733.1. [P05532-1]
NP_066922.2. NM_021099.3. [P05532-2]
UniGeneiMm.247073.

Genome annotation databases

EnsembliENSMUST00000005815; ENSMUSP00000005815; ENSMUSG00000005672. [P05532-1]
GeneIDi16590.
KEGGimmu:16590.
UCSCiuc008xug.2. mouse. [P05532-1]
uc012dxj.1. mouse. [P05532-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y00864 mRNA. Translation: CAA68772.1 .
AK046795 mRNA. Translation: BAC32872.1 .
X65997 mRNA. Translation: CAA46798.1 .
X65998 Genomic DNA. Translation: CAA46799.1 . Sequence problems.
X65998 Genomic DNA. Translation: CAA46800.1 .
AY536430 mRNA. Translation: AAS45606.1 .
AY536431 mRNA. Translation: AAS45607.1 .
AC013622 Genomic DNA. No translation available.
AC115853 Genomic DNA. No translation available.
BC052457 mRNA. Translation: AAH52457.1 .
BC075716 mRNA. Translation: AAH75716.1 .
L11358 Genomic DNA. Translation: AAA37420.1 .
CCDSi CCDS51525.1. [P05532-1 ]
PIRi A44876.
S00474. TVMSKT.
S24667.
S34435.
RefSeqi NP_001116205.1. NM_001122733.1. [P05532-1 ]
NP_066922.2. NM_021099.3. [P05532-2 ]
UniGenei Mm.247073.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2O26 X-ray 2.50 U/W/X/Y 25-314 [» ]
ProteinModelPortali P05532.
SMRi P05532. Positions 33-511, 550-933.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 200957. 18 interactions.
DIPi DIP-59622N.
IntActi P05532. 3 interactions.

Chemistry

ChEMBLi CHEMBL2034798.

PTM databases

PhosphoSitei P05532.

Proteomic databases

MaxQBi P05532.
PaxDbi P05532.
PRIDEi P05532.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000005815 ; ENSMUSP00000005815 ; ENSMUSG00000005672 . [P05532-1 ]
GeneIDi 16590.
KEGGi mmu:16590.
UCSCi uc008xug.2. mouse. [P05532-1 ]
uc012dxj.1. mouse. [P05532-2 ]

Organism-specific databases

CTDi 3815.
MGIi MGI:96677. Kit.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00720000108490.
HOGENOMi HOG000112008.
HOVERGENi HBG004335.
InParanoidi Q6QJB7.
KOi K05091.
TreeFami TF325768.

Enzyme and pathway databases

BRENDAi 2.7.10.1. 3474.
Reactomei REACT_188578. Signaling by SCF-KIT.
REACT_196588. Constitutive PI3K/AKT Signaling in Cancer.
REACT_226341. PIP3 activates AKT signaling.
REACT_227425. Regulation of KIT signaling.

Miscellaneous databases

ChiTaRSi KIT. mouse.
EvolutionaryTracei P05532.
NextBioi 290137.
PROi P05532.
SOURCEi Search...

Gene expression databases

ArrayExpressi P05532.
Bgeei P05532.
CleanExi MM_KIT.
Genevestigatori P05532.

Family and domain databases

Gene3Di 2.60.40.10. 5 hits.
InterProi IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR027263. SCGF_receptor.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
IPR016243. Tyr_kinase_CSF1/PDGF_rcpt.
IPR001824. Tyr_kinase_rcpt_3_CS.
[Graphical view ]
Pfami PF07679. I-set. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
[Graphical view ]
PIRSFi PIRSF500951. SCGF_recepter. 1 hit.
PIRSF000615. TyrPK_CSF1-R. 1 hit.
SMARTi SM00409. IG. 2 hits.
SM00408. IGc2. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 2 hits.
PROSITEi PS50835. IG_LIKE. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS00240. RECEPTOR_TYR_KIN_III. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Primary structure of c-kit: relationship with the CSF-1/PDGF receptor kinase family -- oncogenic activation of v-kit involves deletion of extracellular domain and C-terminus."
    Qiu F., Ray P., Brown K., Barker P.E., Jhanwar S., Ruddle F.H., Besmer P.
    EMBO J. 7:1003-1011(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), VARIANT GLU-207.
    Strain: BALB/c.
    Tissue: Brain.
  2. "Exon skipping by mutation of an authentic splice site of c-kit gene in W/W mouse."
    Hayashi S., Kunisada T., Ogawa M., Yamaguchi K., Nishikawa S.
    Nucleic Acids Res. 19:1267-1271(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Medulla oblongata.
  3. "A novel c-kit transcript, potentially encoding a truncated receptor, originates within a kit gene intron in mouse spermatids."
    Rossi P., Marziali G., Albanesi C., Charlesworth A., Geremia R., Sorrentino V.
    Dev. Biol. 152:203-207(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 3), TISSUE SPECIFICITY.
    Strain: ICR.
  4. "Identification of a novel point mutation of mouse proto-oncogene c-kit through N-ethyl-N-nitrosourea mutagenesis."
    Ruan H.B., Zhang N., Gao X.
    Genetics 169:819-831(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), MUTAGENESIS OF PHE-860.
    Strain: C57BL/6J.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6 and FVB/N.
    Tissue: Brain and Mammary tumor.
  7. "Cloning and functional analysis of the mouse c-kit promoter."
    Yasuda H., Galli S.J., Geissler E.N.
    Biochem. Biophys. Res. Commun. 191:893-901(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-22.
    Strain: BALB/c.
  8. "Candidate ligand for the c-kit transmembrane kinase receptor: KL, a fibroblast derived growth factor stimulates mast cells and erythroid progenitors."
    Tan J.C., Buck J., Levi E., Besmer P.
    EMBO J. 9:3287-3294(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT.
  9. "Signal transduction by normal isoforms and W mutant variants of the Kit receptor tyrosine kinase."
    Reith A.D., Ellis C., Lyman S.D., Anderson D.M., Williams D.E., Bernstein A., Pawson T.
    EMBO J. 10:2451-2459(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN ACTIVATION OF PLCG1, INTERACTION WITH PLCG1, ALTERNATIVE SPLICING, PHOSPHORYLATION, GLYCOSYLATION, CHARACTERIZATION OF VARIANTS W37 LYS-586 AND W41 MET-835.
  10. "Tyrosine residue 719 of the c-kit receptor is essential for binding of the P85 subunit of phosphatidylinositol (PI) 3-kinase and for c-kit-associated PI 3-kinase activity in COS-1 cells."
    Serve H., Hsu Y.C., Besmer P.
    J. Biol. Chem. 269:6026-6030(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PIK3R1, FUNCTION IN PHOSPHORYLATION OF PIK3R1.
  11. "Mechanism of down-regulation of c-kit receptor. Roles of receptor tyrosine kinase, phosphatidylinositol 3'-kinase, and protein kinase C."
    Yee N.S., Hsiau C.W., Serve H., Vosseller K., Besmer P.
    J. Biol. Chem. 269:31991-31998(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: UBIQUITINATION, SUBCELLULAR LOCATION, CATALYTIC ACTIVITY, PHOSPHORYLATION AT TYR-825, INTERACTION WITH PIK3R1, CHARACTERIZATION OF VARIANT W42 ASN-794, MUTAGENESIS OF TYR-723, TISSUE SPECIFICITY.
  12. "Involvement of phospholipase Cgamma1 in mouse egg activation induced by a truncated form of the C-kit tyrosine kinase present in spermatozoa."
    Sette C., Bevilacqua A., Geremia R., Rossi P.
    J. Cell Biol. 142:1063-1074(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: ALTERNATIVE SPLICING, FUNCTION IN ACTIVATION OF PLCG1.
  13. "SHP-1 binds and negatively modulates the c-Kit receptor by interaction with tyrosine 569 in the c-Kit juxtamembrane domain."
    Kozlowski M., Larose L., Lee F., Le D.M., Rottapel R., Siminovitch K.A.
    Mol. Cell. Biol. 18:2089-2099(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PTPN6/SHP-1 AND PTPN11/SHP-2, FUNCTION IN PHOSPHORYLATION OF PTPN6/SHP-1, MUTAGENESIS OF TYR-571 AND TYR-573.
  14. "Molecular mechanisms utilized by alternative c-kit gene products in the control of spermatogonial proliferation and sperm-mediated egg activation."
    Rossi P., Dolci S., Sette C., Geremia R.
    Andrologia 35:71-78(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW ON ROLE IN SPERMATOGENESIS AND FERTILITY.
  15. "The tyrosine kinase FES is an essential effector of KITD816V proliferation signal."
    Voisset E., Lopez S., Dubreuil P., De Sepulveda P.
    Blood 110:2593-2599(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH FES/FPS.
  16. "Quantitative time-resolved phosphoproteomic analysis of mast cell signaling."
    Cao L., Yu K., Banh C., Nguyen V., Ritz A., Raphael B.J., Kawakami Y., Kawakami T., Salomon A.R.
    J. Immunol. 179:5864-5876(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-571; TYR-573; TYR-706 AND TYR-938, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Mast cell.
  17. "Protein-tyrosine phosphatase alpha regulates stem cell factor-dependent c-Kit activation and migration of mast cells."
    Samayawardhena L.A., Pallen C.J.
    J. Biol. Chem. 283:29175-29185(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN MAST CELL MIGRATION, IN SIGNALING VIA FYN.
  18. "Murine hematopoietic stem cells and multipotent progenitors express truncated intracellular form of c-kit receptor."
    Zayas J., Spassov D.S., Nachtman R.G., Jurecic R.
    Stem Cells Dev. 17:343-353(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: ALTERNATIVE SPLICING, TISSUE SPECIFICITY.
  19. "Nf1-/- Schwann cell-conditioned medium modulates mast cell degranulation by c-Kit-mediated hyperactivation of phosphatidylinositol 3-kinase."
    Chen S., Burgin S., McDaniel A., Li X., Yuan J., Chen M., Khalaf W., Clapp D.W., Yang F.C.
    Am. J. Pathol. 177:3125-3132(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN MAST CELL DEGRANULATION.
  20. Jawad-Alam J.
    Unpublished observations (APR-2010)
    Cited for: VARIANT GLU-207.
  21. "Structural basis for stem cell factor-KIT signaling and activation of class III receptor tyrosine kinases."
    Liu H., Chen X., Focia P.J., He X.
    EMBO J. 26:891-901(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 25-314 IN COMPLEX WITH KITLG/SCF, DISULFIDE BONDS, GLYCOSYLATION AT ASN-296 AND ASN-303.
  22. "The dominant W42 spotting phenotype results from a missense mutation in the c-kit receptor kinase."
    Tan J.C., Nocka K., Ray P., Traktman P., Besmer P.
    Science 247:209-212(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: VARIANT W42 ASN-794.
  23. "Molecular bases of dominant negative and loss of function mutations at the murine c-kit/white spotting locus: W37, Wv, W41 and W."
    Nocka K., Tan J.C., Chiu E., Chu T.Y., Ray P., Traktman P., Besmer P.
    EMBO J. 9:1805-1813(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: VARIANTS W37 LYS-586; WV MET-664 AND W41 MET-835.

Entry informationi

Entry nameiKIT_MOUSE
AccessioniPrimary (citable) accession number: P05532
Secondary accession number(s): Q61415
, Q61416, Q61417, Q6LEE9, Q6QJB7, Q6QJB8, Q7TS86, Q8C8K9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: October 19, 2011
Last modified: September 3, 2014
This is version 165 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Numerous proteins are phosphorylated in response to KIT signaling, but it is not evident to determine which are directly phosphorylated by KIT under in vivo conditions.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi