P05527 (HMIN_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Homeobox protein invected

Gene names

Name:	inv
ORF Names:	CG17835

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	576 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Engrailed (en) and invected (inv) are functionally redundant transcription factors in neuronal precursor cell NB5-3 specification. Inv is unable to substitute for en in other regulatory processes such as maintaining gsb expression in the neuroectoderm after stage 10 of embryogenesis. Maintenance of gsb expression in row 5 of the neuroectoderm involves an as yet unidentified short range signaling molecule. Ref.1 Ref.6
Subcellular location	Nucleus Ref.1.
Tissue specificity	Expressed in row 6/7 of the embryonic neuroectoderm. Ref.1 Ref.6
Sequence similarities	Belongs to the engrailed homeobox family. Contains 1 homeobox DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Homeobox
Ligand	DNA-binding
Molecular function	Developmental protein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	compartment pattern specification Traceable author statement. Source: FlyBase neuroblast fate determination Inferred from genetic interaction Ref.6. Source: FlyBase transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW wing disc anterior/posterior pattern formation Traceable author statement. Source: FlyBase
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro transcription regulatory region sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CG17446	Q9W352	1	EBI-100343,EBI-84290
CG3099	Q9W325	1	EBI-100343,EBI-173625
CG9640	Q5U179	1	EBI-100343,EBI-176762
gro	P16371	1	EBI-100343,EBI-153866

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 576	576	Homeobox protein invected		PRO_0000196081
Regions
	DNA binding	471 – 530	60	Homeobox
Experimental info
	Sequence conflict	39	1	G → V in AAL90161. Ref.4
	Sequence conflict	45	1	C → V in AAL90161. Ref.4
	Sequence conflict	81	1	V → A in AAL90161. Ref.4
	Sequence conflict	159	1	L → M in CAA28885. Ref.1
	Sequence conflict	321	1	N → T in CAA28885. Ref.1
	Sequence conflict	321	1	N → T in AAL90161. Ref.4
	Sequence conflict	570	1	A → R in CAA28885. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P05527 [UniParc]. Last modified October 25, 2004. Version 2. Checksum: 67E93DA41A606149	FASTA	576	60,863
	10 20 30 40 50 60 MSTLASTRPP PLKLTIPSLE EAEDHAQERR AGGGGQEVGK MHPDCLPLPL VQPGNSPQVR 70 80 90 100 110 120 EEEEDEQTEC EEQLNIEDEE VEEEHDLDLE DPASCCSENS VLSVGQEQSE AAQAALSAQA 130 140 150 160 170 180 QARQRLLISQ IYRPSAFSST ATTVLPPSEG PPFSPEDLLQ LPPSTGTFQE EFLRKSQLYA 190 200 210 220 230 240 EELMKQQMHL MAAARVNALT AAAAGKQLQM AMAAAAVATV PSGQDALAQL TATALGLGPG 250 260 270 280 290 300 GAVHPHQQLL LQRDQVHHHH HMQNHLNNNE NLHERALKFS IDNILKADFG SRLPKIGALS 310 320 330 340 350 360 GNIGGGSVSG SSTGSSKNSG NTNGNRSPLK APKKSGKPLN LAQSNAAANS SLSFSSSLAN 370 380 390 400 410 420 ICSNSNDSNS TATSSSTTNT SGAPVDLVKS PPPAAGAGAT GASGKSGEDS GTPIVWPAWV 430 440 450 460 470 480 YCTRYSDRPS SGRSPRARKP KKPATSSSAA GGGGGGVEKG EAADGGGVPE DKRPRTAFSG 490 500 510 520 530 540 TQLARLKHEF NENRYLTEKR RQQLSGELGL NEAQIKIWFQ NKRAKLKKSS GTKNPLALQL 550 560 570 MAQGLYNHST IPLTREEEEL QELQEAASAA AAKEPC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The invected gene of Drosophila: sequence analysis and expression studies reveal a close kinship to the engrailed gene." Coleman K.G., Poole S.J., Weir M.P., Soeller W.C., Kornberg T. Genes Dev. 1:19-28(1987) [PubMed: 2892756] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[4]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Testis.
[5]	Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M., Park S., Wan K.H., Yu C., Celniker S.E. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo.
[6]	"Requirement for engrailed and invected genes reveals novel regulatory interactions between engrailed/invected, patched, gooseberry and wingless during Drosophila neurogenesis." Bhat K.M., Schedl P. Development 124:1675-1688(1997) [PubMed: 9165116] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X05273 mRNA. Translation: CAA28885.1. AE013599 Genomic DNA. Translation: AAF58640.3. AE013599 Genomic DNA. Translation: AAM68707.3. AE013599 Genomic DNA. Translation: AAM68708.3. AE013599 Genomic DNA. Translation: AAM68709.2. AY089423 mRNA. Translation: AAL90161.1. BT016097 mRNA. Translation: AAV36982.1.
PIR	A26628.
RefSeq	NP_523699.3. NM_078975.3. NP_725055.2. NM_165837.2. NP_725056.2. NM_165838.2. NP_725057.2. NM_165839.2.
UniGene	Dm.2797.
3D structure databases
ProteinModelPortal	P05527.
SMR	P05527. Positions 487-529.
ModBase	Search...
Protein-protein interaction databases
IntAct	P05527. 5 interactions.
MINT	MINT-314454.
STRING	P05527.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0088067; FBpp0087173; FBgn0001269. FBtr0088068; FBpp0087174; FBgn0001269. FBtr0088069; FBpp0087175; FBgn0001269. FBtr0088070; FBpp0087176; FBgn0001269.
GeneID	36239.
KEGG	dme:Dmel_CG17835.
Organism-specific databases
CTD	36239.
FlyBase	FBgn0001269. inv.
Phylogenomic databases
eggNOG	inNOG04061.
GeneTree	EMGT00070000025675.
InParanoid	P05527.
OMA	KQQMQLM.
OrthoDB	EOG4866W0.
PhylomeDB	P05527.
Gene expression databases
ArrayExpress	P05527.
Bgee	P05527.
GermOnline	CG17835. Drosophila melanogaster.
Family and domain databases
InterPro	IPR001356. Homeobox. IPR000747. Homeobox-engrailed. IPR019549. Homeobox-engrailed_C-terminal. IPR017970. Homeobox_CS. IPR020479. Homeobox_eu. IPR009057. Homeodomain-like. IPR012287. Homeodomain-rel. IPR019737. Homoebox-engrailed_CS. IPR000047. HTH_motif. [Graphical view]
Gene3D	G3DSA:1.10.10.60. Homeodomain-rel. 1 hit.
Pfam	PF10525. Engrail_1_C_sig. 1 hit. PF00046. Homeobox. 1 hit. [Graphical view]
PRINTS	PR00026. ENGRAILED. PR00024. HOMEOBOX. PR00031. HTHREPRESSR.
SMART	SM00389. HOX. 1 hit. [Graphical view]
SUPFAM	SSF46689. Homeodomain_like. 1 hit.
PROSITE	PS00033. ENGRAILED. 1 hit. PS00027. HOMEOBOX_1. 1 hit. PS50071. HOMEOBOX_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	797489.

Entry information

Entry name

HMIN_DROME

Accession

Primary (citable) accession number: P05527
Secondary accession number(s): A4UZD7 , Q0E9C1, Q5U0Z5, Q8T3Z5, Q9V600

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1988
Last sequence update:	October 25, 2004
Last modified:	January 25, 2012
This is version 112 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project