ID   GUN1_PERAE              Reviewed;         494 AA.
AC   P05522;
DT   01-NOV-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1988, sequence version 1.
DT   22-FEB-2023, entry version 98.
DE   RecName: Full=Endoglucanase 1;
DE            EC=3.2.1.4;
DE   AltName: Full=Abscission cellulase 1;
DE   AltName: Full=Endo-1,4-beta-glucanase 1;
DE   Flags: Precursor;
GN   Name=CEL1;
OS   Persea americana (Avocado).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Magnoliidae; Laurales; Lauraceae; Persea.
OX   NCBI_TaxID=3435;
RN   [1]
RP   NUCLEOTIDE SEQUENCE.
RX   AGRICOLA=IND91054589; DOI=10.1007/BF00166456;
RA   Tucker M.L., Durbin M.L., Clegg M.T., Lewis L.N.;
RT   "Avocado cellulase: nucleotide sequence of a putative full-length cDNA
RT   clone and evidence for a small gene family.";
RL   Plant Mol. Biol. 9:197-203(1987).
RN   [2]
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=cv. Hass;
RX   PubMed=2259345; DOI=10.1007/bf00315799;
RA   Cass L.G., Kirven K.A., Christoffersen R.E.;
RT   "Isolation and characterization of a cellulase gene family member expressed
RT   during avocado fruit ripening.";
RL   Mol. Gen. Genet. 223:76-86(1990).
CC   -!- FUNCTION: Involved in ripening fruit process.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC         cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC   -!- DEVELOPMENTAL STAGE: In ripening fruit.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 9 (cellulase E) family.
CC       {ECO:0000255|PROSITE-ProRule:PRU10140, ECO:0000305}.
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DR   EMBL; M17634; AAA32912.1; -; mRNA.
DR   EMBL; X59944; CAA42569.1; -; Genomic_DNA.
DR   PIR; S11946; S11946.
DR   AlphaFoldDB; P05522; -.
DR   SMR; P05522; -.
DR   CAZy; GH9; Glycoside Hydrolase Family 9.
DR   GlyCosmos; P05522; 2 sites, No reported glycans.
DR   GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR   GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0009835; P:fruit ripening; IEA:UniProtKB-KW.
DR   Gene3D; 1.50.10.10; -; 1.
DR   InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR   InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR   InterPro; IPR001701; Glyco_hydro_9.
DR   InterPro; IPR033126; Glyco_hydro_9_Asp/Glu_AS.
DR   InterPro; IPR018221; Glyco_hydro_9_His_AS.
DR   PANTHER; PTHR22298; ENDO-1,4-BETA-GLUCANASE; 1.
DR   PANTHER; PTHR22298:SF29; ENDOGLUCANASE; 1.
DR   Pfam; PF00759; Glyco_hydro_9; 1.
DR   SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
DR   PROSITE; PS60032; GH9_1; 1.
DR   PROSITE; PS00592; GH9_2; 1.
DR   PROSITE; PS00698; GH9_3; 1.
PE   2: Evidence at transcript level;
KW   Carbohydrate metabolism; Cellulose degradation; Fruit ripening;
KW   Glycoprotein; Glycosidase; Hydrolase; Polysaccharide degradation; Signal.
FT   SIGNAL          1..25
FT   CHAIN           26..494
FT                   /note="Endoglucanase 1"
FT                   /id="PRO_0000007955"
FT   ACT_SITE        82
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10140"
FT   ACT_SITE        411
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10059"
FT   ACT_SITE        462
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10060"
FT   ACT_SITE        471
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10060"
FT   CARBOHYD        254
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        359
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   494 AA;  54079 MW;  763009AD15575C31 CRC64;
     MDCSSPLSLF HLLLVCTVMV KCCSASDLHY SDALEKSILF FEGQRSGKLP TNQRLTWRGD
     SGLSDGSSYH VDLVGGYYDA GDNLKFGLPM AFTTTMLAWG IIEFGCLMPE QVENARAALR
     WSTDYLLKAS TATSNSLYVQ VGEPNADHRC WERPEDMDTP RNVYKVSTQN PGSDVAAETA
     AALAAASIVF GDSDSSYSTK LLHTAVKVFE FADQYRGSYS DSLGSVVCPF YCSYSGYNDE
     LLWGASWLHR ASQNASYMTY IQSNGHTLGA DDDDYSFSWD DKRVGTKVLL SKGFLQDRIE
     ELQLYKVHTD NYICSLIPGT SSFQAQYTPG GLLYKGSASN LQYVTSTAFL LLTYANYLNS
     SGGHASCGTT TVTAKNLISL AKKQVDYILG QNPAKMSYMV GFGERYPQHV HHRGSSLPSV
     QVHPNSIPCN AGFQYLYSSP PNPNILVGAI LGGPDNRDSF SDDRNNYQQS EPATYINAPL
     VGALAFFAAN PVTE
//