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P05522

- GUN1_PERAE

UniProt

P05522 - GUN1_PERAE

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Protein
Endoglucanase 1
Gene
CEL1
Organism
Persea americana (Avocado)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Involved in ripening fruit process.

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei411 – 4111 By similarity
Active sitei462 – 4621 By similarity
Active sitei471 – 4711 By similarity

GO - Molecular functioni

  1. cellulase activity Source: UniProtKB-EC

GO - Biological processi

  1. cellulose catabolic process Source: UniProtKB-KW
  2. fruit ripening Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cellulose degradation, Fruit ripening, Polysaccharide degradation

Protein family/group databases

CAZyiGH9. Glycoside Hydrolase Family 9.

Names & Taxonomyi

Protein namesi
Recommended name:
Endoglucanase 1 (EC:3.2.1.4)
Alternative name(s):
Abscission cellulase 1
Endo-1,4-beta-glucanase 1
Gene namesi
Name:CEL1
OrganismiPersea americana (Avocado)
Taxonomic identifieri3435 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaMagnoliidaeLauralesLauraceaePersea

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525
Add
BLAST
Chaini26 – 494469Endoglucanase 1
PRO_0000007955Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi254 – 2541N-linked (GlcNAc...) Reviewed prediction
Glycosylationi359 – 3591N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein

Expressioni

Developmental stagei

In ripening fruit.

Structurei

3D structure databases

ProteinModelPortaliP05522.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR001701. Glyco_hydro_9.
IPR018221. Glyco_hydro_9_AS.
[Graphical view]
PfamiPF00759. Glyco_hydro_9. 1 hit.
[Graphical view]
SUPFAMiSSF48208. SSF48208. 1 hit.
PROSITEiPS00592. GLYCOSYL_HYDROL_F9_1. 1 hit.
PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P05522-1 [UniParc]FASTAAdd to Basket

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MDCSSPLSLF HLLLVCTVMV KCCSASDLHY SDALEKSILF FEGQRSGKLP    50
TNQRLTWRGD SGLSDGSSYH VDLVGGYYDA GDNLKFGLPM AFTTTMLAWG 100
IIEFGCLMPE QVENARAALR WSTDYLLKAS TATSNSLYVQ VGEPNADHRC 150
WERPEDMDTP RNVYKVSTQN PGSDVAAETA AALAAASIVF GDSDSSYSTK 200
LLHTAVKVFE FADQYRGSYS DSLGSVVCPF YCSYSGYNDE LLWGASWLHR 250
ASQNASYMTY IQSNGHTLGA DDDDYSFSWD DKRVGTKVLL SKGFLQDRIE 300
ELQLYKVHTD NYICSLIPGT SSFQAQYTPG GLLYKGSASN LQYVTSTAFL 350
LLTYANYLNS SGGHASCGTT TVTAKNLISL AKKQVDYILG QNPAKMSYMV 400
GFGERYPQHV HHRGSSLPSV QVHPNSIPCN AGFQYLYSSP PNPNILVGAI 450
LGGPDNRDSF SDDRNNYQQS EPATYINAPL VGALAFFAAN PVTE 494
Length:494
Mass (Da):54,079
Last modified:November 1, 1988 - v1
Checksum:i763009AD15575C31
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M17634 mRNA. Translation: AAA32912.1.
X59944 Genomic DNA. Translation: CAA42569.1.
PIRiS11946.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M17634 mRNA. Translation: AAA32912.1 .
X59944 Genomic DNA. Translation: CAA42569.1 .
PIRi S11946.

3D structure databases

ProteinModelPortali P05522.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH9. Glycoside Hydrolase Family 9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 1.50.10.10. 1 hit.
InterProi IPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR001701. Glyco_hydro_9.
IPR018221. Glyco_hydro_9_AS.
[Graphical view ]
Pfami PF00759. Glyco_hydro_9. 1 hit.
[Graphical view ]
SUPFAMi SSF48208. SSF48208. 1 hit.
PROSITEi PS00592. GLYCOSYL_HYDROL_F9_1. 1 hit.
PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Avocado cellulase: nucleotide sequence of a putative full-length cDNA clone and evidence for a small gene family."
    Tucker M.L., Durbin M.L., Clegg M.T., Lewis L.N.
    Plant Mol. Biol. 9:197-203(1987)
    [AGRICOLA] [Europe PMC]
    Cited for: NUCLEOTIDE SEQUENCE.
  2. "Isolation and characterization of a cellulase gene family member expressed during avocado fruit ripening."
    Cass L.G., Kirven K.A., Christoffersen R.E.
    Mol. Gen. Genet. 223:76-86(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: cv. Hass.

Entry informationi

Entry nameiGUN1_PERAE
AccessioniPrimary (citable) accession number: P05522
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: November 1, 1988
Last modified: November 13, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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