Reviewed, UniProtKB/Swiss-Prot P05522 (GUN1_PERAE)

Names and origin

Protein names

Recommended name:
    Endoglucanase 1
    EC=3.2.1.4
Alternative name(s):
    Endo-1,4-beta-glucanase 1
    Abscission cellulase 1

Gene names

Name:

CEL1

Organism

Persea americana (Avocado)

Taxonomic identifier

3435 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › magnoliids › Laurales › Lauraceae › Persea

Protein attributes

Sequence length	494 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Involved in ripening fruit process.
Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Developmental stage	In ripening fruit.
Sequence similarities	Belongs to the glycosyl hydrolase 9 (cellulase E) family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cellulose degradation Fruit ripening Polysaccharide degradation
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW ripening Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	cellulase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25
	Chain	26 – 494	469	Endoglucanase 1		PRO_0000007955
Sites
	Active site	411	1	By similarity
	Active site	462	1	By similarity
	Active site	471	1	By similarity
Amino acid modifications
	Glycosylation	254	1	N-linked (GlcNAc...) Potential
	Glycosylation	359	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P05522-1 [UniParc]. Last modified November 1, 1988. Version 1. Checksum: 763009AD15575C31	FASTA	494	54,079
	10 20 30 40 50 60 MDCSSPLSLF HLLLVCTVMV KCCSASDLHY SDALEKSILF FEGQRSGKLP TNQRLTWRGD 70 80 90 100 110 120 SGLSDGSSYH VDLVGGYYDA GDNLKFGLPM AFTTTMLAWG IIEFGCLMPE QVENARAALR 130 140 150 160 170 180 WSTDYLLKAS TATSNSLYVQ VGEPNADHRC WERPEDMDTP RNVYKVSTQN PGSDVAAETA 190 200 210 220 230 240 AALAAASIVF GDSDSSYSTK LLHTAVKVFE FADQYRGSYS DSLGSVVCPF YCSYSGYNDE 250 260 270 280 290 300 LLWGASWLHR ASQNASYMTY IQSNGHTLGA DDDDYSFSWD DKRVGTKVLL SKGFLQDRIE 310 320 330 340 350 360 ELQLYKVHTD NYICSLIPGT SSFQAQYTPG GLLYKGSASN LQYVTSTAFL LLTYANYLNS 370 380 390 400 410 420 SGGHASCGTT TVTAKNLISL AKKQVDYILG QNPAKMSYMV GFGERYPQHV HHRGSSLPSV 430 440 450 460 470 480 QVHPNSIPCN AGFQYLYSSP PNPNILVGAI LGGPDNRDSF SDDRNNYQQS EPATYINAPL 490 VGALAFFAAN PVTE

References

[1]	"Avocado cellulase: nucleotide sequence of a putative full-length cDNA clone and evidence for a small gene family." Tucker M.L., Durbin M.L., Clegg M.T., Lewis L.N. Plant Mol. Biol. 9:197-203(1987) [Agricola: IND91054589] Cited for: NUCLEOTIDE SEQUENCE.
[2]	"Isolation and characterization of a cellulase gene family member expressed during avocado fruit ripening." Cass L.G., Kirven K.A., Christoffersen R.E. Mol. Gen. Genet. 223:76-86(1990) [PubMed: 2259345] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: cv. Hass.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M17634 mRNA. Translation: AAA32912.1. X59944 Genomic DNA. Translation: CAA42569.1.
PIR	S11946.
3D structure databases
HSSP	HSSP built from PDB template 1IA6 based on UniProtKB Q9EYQ2.
ModBase	Search...
Protein family/group databases
CAZy	GH9. Glycoside Hydrolase Family 9.
Enzyme and pathway databases
BRENDA	3.2.1.4. 2559.
Family and domain databases
InterPro	IPR012341. 6hp_glycosidase. IPR001701. Glyco_hydro_9. IPR018221. Glyco_hydro_9_AS. [Graphical view]
Gene3D	G3DSA:1.50.10.10. CelA/Cel48F_cat. 1 hit.
PANTHER	PTHR22298:SF3. Glyco_hydro_9. 1 hit.
Pfam	PF00759. Glyco_hydro_9. 1 hit. [Graphical view]
PROSITE	PS00592. GLYCOSYL_HYDROL_F9_1. 1 hit. PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUN1_PERAE

Accession

Primary (citable) accession number: P05522

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1988
Last sequence update:	November 1, 1988
Last modified:	May 5, 2009
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)