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Protein

Acyl transferase

Gene

luxD

Organism
Vibrio harveyi (Beneckea harveyi)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Acyl transferase is part of the fatty acid reductase system required for aldehyde biosynthesis; it produces fatty acids for the luminescent reaction.

Pathwayi: fatty acid reduction for biolumincescence

This protein is involved in the pathway fatty acid reduction for biolumincescence, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway fatty acid reduction for biolumincescence and in Lipid metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei114 – 1141Charge relay system
Active sitei211 – 2111Charge relay system
Active sitei241 – 2411Charge relay system

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Luminescence

Enzyme and pathway databases

UniPathwayiUPA00569.

Protein family/group databases

ESTHERivibha-1luxd. Thioesterase_acyl-transferase.

Names & Taxonomyi

Protein namesi
Recommended name:
Acyl transferase (EC:2.3.1.-)
Short name:
ACT
Alternative name(s):
C14ACP-TE
Myristoyl-ACP-specific thioesterase
Gene namesi
Name:luxD
OrganismiVibrio harveyi (Beneckea harveyi)
Taxonomic identifieri669 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 305305Acyl transferasePRO_0000220195Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi338187.VIBHAR_06243.

Structurei

Secondary structure

1
305
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi8 – 147Combined sources
Turni15 – 173Combined sources
Beta strandi18 – 258Combined sources
Beta strandi37 – 415Combined sources
Helixi46 – 516Combined sources
Helixi52 – 598Combined sources
Turni60 – 623Combined sources
Beta strandi65 – 684Combined sources
Helixi86 – 10217Combined sources
Beta strandi108 – 1136Combined sources
Helixi116 – 1238Combined sources
Turni124 – 1263Combined sources
Beta strandi130 – 1367Combined sources
Helixi141 – 1499Combined sources
Helixi153 – 1553Combined sources
Helixi158 – 1603Combined sources
Beta strandi163 – 1675Combined sources
Beta strandi170 – 1734Combined sources
Helixi174 – 18310Combined sources
Helixi189 – 1968Combined sources
Beta strandi203 – 2086Combined sources
Helixi216 – 2238Combined sources
Beta strandi231 – 2366Combined sources
Helixi247 – 26519Combined sources
Helixi280 – 29617Combined sources
Helixi297 – 2993Combined sources
Beta strandi302 – 3043Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1THTX-ray2.10A/B1-305[»]
ProteinModelPortaliP05521.
SMRiP05521. Positions 4-305.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP05521.

Family & Domainsi

Sequence similaritiesi

Belongs to the LuxD family.Curated

Phylogenomic databases

eggNOGiENOG4108XDE. Bacteria.
ENOG4111MFF. LUCA.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
HAMAPiMF_00774. LuxD.
InterProiIPR029058. AB_hydrolase.
IPR003157. LuxD.
[Graphical view]
PfamiPF02273. Acyl_transf_2. 1 hit.
[Graphical view]
PIRSFiPIRSF009416. LuxD. 1 hit.
SUPFAMiSSF53474. SSF53474. 1 hit.

Sequencei

Sequence statusi: Complete.

P05521-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNNQCKTIAH VLRVNNGQEL HVWETPPKEN VPFKNNTILI ASGFARRMDH
60 70 80 90 100
FAGLAEYLSE NGFHVFRYDS LHHVGLSSGS IDEFTMTTGK NSLCTVYHWL
110 120 130 140 150
QTKGTQNIGL IAASLSARVA YEVISDLELS FLITAVGVVN LRDTLEKALG
160 170 180 190 200
FDYLSLPIDE LPNDLDFEGH KLGSEVFVRD CFEHHWDTLD STLDKVANTS
210 220 230 240 250
VPLIAFTANN DDWVKQEEVY DMLAHIRTGH CKLYSLLGSS HDLGENLVVL
260 270 280 290 300
RNFYQSVTKA AIAMDGGSLE IDVDFIEPDF EQLTIATVNE RRLKAEIESR

TPEMA
Length:305
Mass (Da):34,207
Last modified:August 1, 1990 - v2
Checksum:i316B694A0E864AD9
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti75 – 751G → E in mutant M17.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03950 Genomic DNA. Translation: AAA27535.1.
M10961 Genomic DNA. Translation: AAA88684.1.
PIRiA28947.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03950 Genomic DNA. Translation: AAA27535.1.
M10961 Genomic DNA. Translation: AAA88684.1.
PIRiA28947.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1THTX-ray2.10A/B1-305[»]
ProteinModelPortaliP05521.
SMRiP05521. Positions 4-305.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi338187.VIBHAR_06243.

Protein family/group databases

ESTHERivibha-1luxd. Thioesterase_acyl-transferase.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG4108XDE. Bacteria.
ENOG4111MFF. LUCA.

Enzyme and pathway databases

UniPathwayiUPA00569.

Miscellaneous databases

EvolutionaryTraceiP05521.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
HAMAPiMF_00774. LuxD.
InterProiIPR029058. AB_hydrolase.
IPR003157. LuxD.
[Graphical view]
PfamiPF02273. Acyl_transf_2. 1 hit.
[Graphical view]
PIRSFiPIRSF009416. LuxD. 1 hit.
SUPFAMiSSF53474. SSF53474. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Organization of the lux structural genes of Vibrio harveyi. Expression under the T7 bacteriophage promoter, mRNA analysis, and nucleotide sequence of the luxD gene."
    Miyamoto C., Boylan M., Graham A.F., Meighen E.A.
    J. Biol. Chem. 263:13393-13399(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Nucleotide sequence of the luxA gene of Vibrio harveyi and the complete amino acid sequence of the alpha subunit of bacterial luciferase."
    Cohn D.H., Mileham A.J., Simon M.I., Nealson K.H., Rausch S.K., Bonam D., Baldwin T.O.
    J. Biol. Chem. 260:6139-6146(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 83-305.
  3. "Structure of a myristoyl-ACP-specific thioesterase from Vibrio harveyi."
    Lawson D.M., Derewenda U., Serre L., Ferri S., Szittner R., Wei Y., Meighen E.A., Derewenda Z.S.
    Biochemistry 33:9382-9388(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS).

Entry informationi

Entry nameiLUXD_VIBHA
AccessioniPrimary (citable) accession number: P05521
Secondary accession number(s): P11002
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: August 1, 1990
Last modified: November 11, 2015
This is version 77 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.